A new group-training procedure for habituation demonstrates that presynaptic glutamate release contributes to long-term memory in Caenorhabditis elegans

In the experiments reported here we have developed a new group-training protocol for assessing long-term memory for habituation in Caenorhabditis elegans. We have replicated all of the major findings of the original single-worm protocol using the new protocol: (1) distributed training produced long-...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Learning & memory (Cold Spring Harbor, N.Y.) N.Y.), 2002-05, Vol.9 (3), p.130-137
Hauptverfasser: Rose, Jacqueline K, Kaun, Karla R, Rankin, Catharine H
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 137
container_issue 3
container_start_page 130
container_title Learning & memory (Cold Spring Harbor, N.Y.)
container_volume 9
creator Rose, Jacqueline K
Kaun, Karla R
Rankin, Catharine H
description In the experiments reported here we have developed a new group-training protocol for assessing long-term memory for habituation in Caenorhabditis elegans. We have replicated all of the major findings of the original single-worm protocol using the new protocol: (1) distributed training produced long-term retention of training, massed training did not; (2) distributed training at long interstimulus intervals (ISIs) produced long-term retention, short ISIs did not; and (3) long-term memory for distributed training is protein synthesis-dependent as it could be blocked by heat shock during the inter-block interval. In addition, we have shown that long-term memory for habituation is graded, depending on the number of blocks of stimuli in training. The inter-block interval must be >40 min for long-term retention of training to occur. Finally, we have tested long-term memory for habituation training in a strain of worms with a mutation in a vesicular glutamate transporter in the sensory neurons that transduce tap (eat-4). The results from these eat-4 worms indicate that glutamate release from the sensory neurons has an important role in the formation of long-term memory for habituation.
doi_str_mv 10.1101/lm.46802
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_182588</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>18733582</sourcerecordid><originalsourceid>FETCH-LOGICAL-c398t-affbf08428f38a41340fe0a060a762c7192b9222de86210914301b1e2eb3f4c73</originalsourceid><addsrcrecordid>eNqFkc1u1TAQhSMEon9IPAHyCrFJGdtJ7CxYVFdAkSp1Q9eW44xzjRI72A7VfRMet6a94mfFakaa75w50qmq1xQuKQX6fl4um04Ce1ad0rbp67aR7fOyg2A1tMBOqrOUvgGAEA19WZ1QBqIFoKfVzyvi8Z5MMWxrnaN23vmJrDEYHLeIxIZI9npwedPZBU9GXIJPBcyYSN7rXFhMB6_X7AyZ5i3rpdxIxBl1QmKCz9EN2yMeyBz8VGeMC1mKUTwQ58lOow-xPBlddokU4aR9uqheWD0nfHWc59Xdp49fd9f1ze3nL7urm9rwXuZaWztYkA2TlkvdUN6ARdDQgRYdM4L2bOgZYyPKjlHoacOBDhQZDtw2RvDz6sOT77oNC44GS149qzW6RceDCtqpfy_e7dUUfigqWStl0b896mP4vmHKanHJ4Dxrj2FLSlDJu47Df0EqBeetZAV89wSaGFKKaH-HoaB-1a3mRT3WXdA3f4f_Ax775Q8c_qpn</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18733582</pqid></control><display><type>article</type><title>A new group-training procedure for habituation demonstrates that presynaptic glutamate release contributes to long-term memory in Caenorhabditis elegans</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Rose, Jacqueline K ; Kaun, Karla R ; Rankin, Catharine H</creator><creatorcontrib>Rose, Jacqueline K ; Kaun, Karla R ; Rankin, Catharine H</creatorcontrib><description>In the experiments reported here we have developed a new group-training protocol for assessing long-term memory for habituation in Caenorhabditis elegans. We have replicated all of the major findings of the original single-worm protocol using the new protocol: (1) distributed training produced long-term retention of training, massed training did not; (2) distributed training at long interstimulus intervals (ISIs) produced long-term retention, short ISIs did not; and (3) long-term memory for distributed training is protein synthesis-dependent as it could be blocked by heat shock during the inter-block interval. In addition, we have shown that long-term memory for habituation is graded, depending on the number of blocks of stimuli in training. The inter-block interval must be &gt;40 min for long-term retention of training to occur. Finally, we have tested long-term memory for habituation training in a strain of worms with a mutation in a vesicular glutamate transporter in the sensory neurons that transduce tap (eat-4). The results from these eat-4 worms indicate that glutamate release from the sensory neurons has an important role in the formation of long-term memory for habituation.</description><identifier>ISSN: 1072-0502</identifier><identifier>EISSN: 1549-5485</identifier><identifier>DOI: 10.1101/lm.46802</identifier><identifier>PMID: 12075001</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>Animals ; Caenorhabditis elegans - physiology ; Conditioning (Psychology) - physiology ; Glutamic Acid - metabolism ; Habituation, Psychophysiologic - physiology ; Memory - physiology ; Presynaptic Terminals - metabolism ; Research Paper ; Synaptic Transmission - physiology</subject><ispartof>Learning &amp; memory (Cold Spring Harbor, N.Y.), 2002-05, Vol.9 (3), p.130-137</ispartof><rights>Copyright © 2002, Cold Spring Harbor Laboratory Press 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c398t-affbf08428f38a41340fe0a060a762c7192b9222de86210914301b1e2eb3f4c73</citedby><cites>FETCH-LOGICAL-c398t-affbf08428f38a41340fe0a060a762c7192b9222de86210914301b1e2eb3f4c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC182588/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC182588/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12075001$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rose, Jacqueline K</creatorcontrib><creatorcontrib>Kaun, Karla R</creatorcontrib><creatorcontrib>Rankin, Catharine H</creatorcontrib><title>A new group-training procedure for habituation demonstrates that presynaptic glutamate release contributes to long-term memory in Caenorhabditis elegans</title><title>Learning &amp; memory (Cold Spring Harbor, N.Y.)</title><addtitle>Learn Mem</addtitle><description>In the experiments reported here we have developed a new group-training protocol for assessing long-term memory for habituation in Caenorhabditis elegans. We have replicated all of the major findings of the original single-worm protocol using the new protocol: (1) distributed training produced long-term retention of training, massed training did not; (2) distributed training at long interstimulus intervals (ISIs) produced long-term retention, short ISIs did not; and (3) long-term memory for distributed training is protein synthesis-dependent as it could be blocked by heat shock during the inter-block interval. In addition, we have shown that long-term memory for habituation is graded, depending on the number of blocks of stimuli in training. The inter-block interval must be &gt;40 min for long-term retention of training to occur. Finally, we have tested long-term memory for habituation training in a strain of worms with a mutation in a vesicular glutamate transporter in the sensory neurons that transduce tap (eat-4). The results from these eat-4 worms indicate that glutamate release from the sensory neurons has an important role in the formation of long-term memory for habituation.</description><subject>Animals</subject><subject>Caenorhabditis elegans - physiology</subject><subject>Conditioning (Psychology) - physiology</subject><subject>Glutamic Acid - metabolism</subject><subject>Habituation, Psychophysiologic - physiology</subject><subject>Memory - physiology</subject><subject>Presynaptic Terminals - metabolism</subject><subject>Research Paper</subject><subject>Synaptic Transmission - physiology</subject><issn>1072-0502</issn><issn>1549-5485</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAQhSMEon9IPAHyCrFJGdtJ7CxYVFdAkSp1Q9eW44xzjRI72A7VfRMet6a94mfFakaa75w50qmq1xQuKQX6fl4um04Ce1ad0rbp67aR7fOyg2A1tMBOqrOUvgGAEA19WZ1QBqIFoKfVzyvi8Z5MMWxrnaN23vmJrDEYHLeIxIZI9npwedPZBU9GXIJPBcyYSN7rXFhMB6_X7AyZ5i3rpdxIxBl1QmKCz9EN2yMeyBz8VGeMC1mKUTwQ58lOow-xPBlddokU4aR9uqheWD0nfHWc59Xdp49fd9f1ze3nL7urm9rwXuZaWztYkA2TlkvdUN6ARdDQgRYdM4L2bOgZYyPKjlHoacOBDhQZDtw2RvDz6sOT77oNC44GS149qzW6RceDCtqpfy_e7dUUfigqWStl0b896mP4vmHKanHJ4Dxrj2FLSlDJu47Df0EqBeetZAV89wSaGFKKaH-HoaB-1a3mRT3WXdA3f4f_Ax775Q8c_qpn</recordid><startdate>20020501</startdate><enddate>20020501</enddate><creator>Rose, Jacqueline K</creator><creator>Kaun, Karla R</creator><creator>Rankin, Catharine H</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7TK</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20020501</creationdate><title>A new group-training procedure for habituation demonstrates that presynaptic glutamate release contributes to long-term memory in Caenorhabditis elegans</title><author>Rose, Jacqueline K ; Kaun, Karla R ; Rankin, Catharine H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c398t-affbf08428f38a41340fe0a060a762c7192b9222de86210914301b1e2eb3f4c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Caenorhabditis elegans - physiology</topic><topic>Conditioning (Psychology) - physiology</topic><topic>Glutamic Acid - metabolism</topic><topic>Habituation, Psychophysiologic - physiology</topic><topic>Memory - physiology</topic><topic>Presynaptic Terminals - metabolism</topic><topic>Research Paper</topic><topic>Synaptic Transmission - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rose, Jacqueline K</creatorcontrib><creatorcontrib>Kaun, Karla R</creatorcontrib><creatorcontrib>Rankin, Catharine H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Learning &amp; memory (Cold Spring Harbor, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rose, Jacqueline K</au><au>Kaun, Karla R</au><au>Rankin, Catharine H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new group-training procedure for habituation demonstrates that presynaptic glutamate release contributes to long-term memory in Caenorhabditis elegans</atitle><jtitle>Learning &amp; memory (Cold Spring Harbor, N.Y.)</jtitle><addtitle>Learn Mem</addtitle><date>2002-05-01</date><risdate>2002</risdate><volume>9</volume><issue>3</issue><spage>130</spage><epage>137</epage><pages>130-137</pages><issn>1072-0502</issn><eissn>1549-5485</eissn><abstract>In the experiments reported here we have developed a new group-training protocol for assessing long-term memory for habituation in Caenorhabditis elegans. We have replicated all of the major findings of the original single-worm protocol using the new protocol: (1) distributed training produced long-term retention of training, massed training did not; (2) distributed training at long interstimulus intervals (ISIs) produced long-term retention, short ISIs did not; and (3) long-term memory for distributed training is protein synthesis-dependent as it could be blocked by heat shock during the inter-block interval. In addition, we have shown that long-term memory for habituation is graded, depending on the number of blocks of stimuli in training. The inter-block interval must be &gt;40 min for long-term retention of training to occur. Finally, we have tested long-term memory for habituation training in a strain of worms with a mutation in a vesicular glutamate transporter in the sensory neurons that transduce tap (eat-4). The results from these eat-4 worms indicate that glutamate release from the sensory neurons has an important role in the formation of long-term memory for habituation.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>12075001</pmid><doi>10.1101/lm.46802</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1072-0502
ispartof Learning & memory (Cold Spring Harbor, N.Y.), 2002-05, Vol.9 (3), p.130-137
issn 1072-0502
1549-5485
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_182588
source MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central
subjects Animals
Caenorhabditis elegans - physiology
Conditioning (Psychology) - physiology
Glutamic Acid - metabolism
Habituation, Psychophysiologic - physiology
Memory - physiology
Presynaptic Terminals - metabolism
Research Paper
Synaptic Transmission - physiology
title A new group-training procedure for habituation demonstrates that presynaptic glutamate release contributes to long-term memory in Caenorhabditis elegans
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T03%3A57%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20new%20group-training%20procedure%20for%20habituation%20demonstrates%20that%20presynaptic%20glutamate%20release%20contributes%20to%20long-term%20memory%20in%20Caenorhabditis%20elegans&rft.jtitle=Learning%20&%20memory%20(Cold%20Spring%20Harbor,%20N.Y.)&rft.au=Rose,%20Jacqueline%20K&rft.date=2002-05-01&rft.volume=9&rft.issue=3&rft.spage=130&rft.epage=137&rft.pages=130-137&rft.issn=1072-0502&rft.eissn=1549-5485&rft_id=info:doi/10.1101/lm.46802&rft_dat=%3Cproquest_pubme%3E18733582%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18733582&rft_id=info:pmid/12075001&rfr_iscdi=true