Interleukin‐18 overexpression as a hallmark of the activity of autoimmune inflammatory myopathies
Summary The objective of this study was to explore the role of interleukin (IL)‐18 in patients with inflammatory myopathies (IM) such as dermatomyositis (DM) and polymyositis (PM) in relation to the possible predominance of a Th1 immune response in their pathogenesis. Serum concentrations of IL‐18,...
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| Veröffentlicht in: | Clinical and experimental immunology 2006-10, Vol.146 (1), p.21-31 |
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| creator | Tucci, M. Quatraro, C. Dammacco, F. Silvestris, F. |
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The objective of this study was to explore the role of interleukin (IL)‐18 in patients with inflammatory myopathies (IM) such as dermatomyositis (DM) and polymyositis (PM) in relation to the possible predominance of a Th1 immune response in their pathogenesis. Serum concentrations of IL‐18, interferon (IFN)‐γ, IL‐4 and IL‐6 were measured in six patients by enzyme‐linked immunosorbent assay (ELISA). IL‐18 expression was evaluated by in situ hybridization (ISH), whereas CD68, CD8 and CD83 were investigated by immunohistochemistry (IHC) to define the main producers of IL‐18. Lastly, the expression of both IL‐18 receptor (IL‐18R) and monocyte chemoattractant protein (MCP)‐1 was also explored by IHC. High serum levels of IL‐18 and IFN‐γ, and conversely low titres of IL‐4 and IL‐6, were demonstrated in both diseases. In addition, IL‐18 was overexpressed in muscle biopsy specimens from patients with IM. Both macrophages and dendritic cells (DC) surrounding either perivascular and perimysium areas in DM or endomysium in PM were the main producers of IL‐18. Endothelial cells (EC), smooth muscle cells (SMC) and CD8+ T cells expressed a high content of IL‐18R. Vessel cells overexpressed MCP‐1 in parallel with IL‐18R. High concentrations of serum IL‐18 as well as muscular up‐regulation of IL‐18 and IL‐18R suggest that deregulation of the IL‐18/IL‐18R pathway is a pathogenetic mechanism in IM. Measurement of IL‐18 may thus predict the severity of both DM and PM. |
| doi_str_mv | 10.1111/j.1365-2249.2006.03180.x |
| format | Article |
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The objective of this study was to explore the role of interleukin (IL)‐18 in patients with inflammatory myopathies (IM) such as dermatomyositis (DM) and polymyositis (PM) in relation to the possible predominance of a Th1 immune response in their pathogenesis. Serum concentrations of IL‐18, interferon (IFN)‐γ, IL‐4 and IL‐6 were measured in six patients by enzyme‐linked immunosorbent assay (ELISA). IL‐18 expression was evaluated by in situ hybridization (ISH), whereas CD68, CD8 and CD83 were investigated by immunohistochemistry (IHC) to define the main producers of IL‐18. Lastly, the expression of both IL‐18 receptor (IL‐18R) and monocyte chemoattractant protein (MCP)‐1 was also explored by IHC. High serum levels of IL‐18 and IFN‐γ, and conversely low titres of IL‐4 and IL‐6, were demonstrated in both diseases. In addition, IL‐18 was overexpressed in muscle biopsy specimens from patients with IM. Both macrophages and dendritic cells (DC) surrounding either perivascular and perimysium areas in DM or endomysium in PM were the main producers of IL‐18. Endothelial cells (EC), smooth muscle cells (SMC) and CD8+ T cells expressed a high content of IL‐18R. Vessel cells overexpressed MCP‐1 in parallel with IL‐18R. High concentrations of serum IL‐18 as well as muscular up‐regulation of IL‐18 and IL‐18R suggest that deregulation of the IL‐18/IL‐18R pathway is a pathogenetic mechanism in IM. Measurement of IL‐18 may thus predict the severity of both DM and PM.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>DOI: 10.1111/j.1365-2249.2006.03180.x</identifier><identifier>PMID: 16968394</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Biological and medical sciences ; CD8-Positive T-Lymphocytes - immunology ; Chemokine CCL2 - metabolism ; Clinical Studies ; dendritic cells ; Dendritic Cells - immunology ; Dermatomyositis - immunology ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunopathology ; inflammation ; Interferon-gamma - blood ; Interleukin-18 - blood ; Interleukin-18 - metabolism ; Interleukin-4 - blood ; Interleukin-6 - blood ; interleukin‐18 ; Macrophages - immunology ; Male ; MCP‐1 ; Medical sciences ; Middle Aged ; Muscle, Skeletal - immunology ; myositis ; Myositis - immunology ; Polymyositis - immunology ; Receptors, Interleukin-18 - metabolism ; Th1 Cells - immunology</subject><ispartof>Clinical and experimental immunology, 2006-10, Vol.146 (1), p.21-31</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Oct 2006</rights><rights>2006 British Society for Immunology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6260-30aabc487d2352f79e91619ae67917d496df195cfbcef819897dcbdae5ed94af3</citedby><cites>FETCH-LOGICAL-c6260-30aabc487d2352f79e91619ae67917d496df195cfbcef819897dcbdae5ed94af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1809738/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1809738/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27913,27914,53780,53782</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18120437$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16968394$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tucci, M.</creatorcontrib><creatorcontrib>Quatraro, C.</creatorcontrib><creatorcontrib>Dammacco, F.</creatorcontrib><creatorcontrib>Silvestris, F.</creatorcontrib><title>Interleukin‐18 overexpression as a hallmark of the activity of autoimmune inflammatory myopathies</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>Summary
The objective of this study was to explore the role of interleukin (IL)‐18 in patients with inflammatory myopathies (IM) such as dermatomyositis (DM) and polymyositis (PM) in relation to the possible predominance of a Th1 immune response in their pathogenesis. Serum concentrations of IL‐18, interferon (IFN)‐γ, IL‐4 and IL‐6 were measured in six patients by enzyme‐linked immunosorbent assay (ELISA). IL‐18 expression was evaluated by in situ hybridization (ISH), whereas CD68, CD8 and CD83 were investigated by immunohistochemistry (IHC) to define the main producers of IL‐18. Lastly, the expression of both IL‐18 receptor (IL‐18R) and monocyte chemoattractant protein (MCP)‐1 was also explored by IHC. High serum levels of IL‐18 and IFN‐γ, and conversely low titres of IL‐4 and IL‐6, were demonstrated in both diseases. In addition, IL‐18 was overexpressed in muscle biopsy specimens from patients with IM. Both macrophages and dendritic cells (DC) surrounding either perivascular and perimysium areas in DM or endomysium in PM were the main producers of IL‐18. Endothelial cells (EC), smooth muscle cells (SMC) and CD8+ T cells expressed a high content of IL‐18R. Vessel cells overexpressed MCP‐1 in parallel with IL‐18R. High concentrations of serum IL‐18 as well as muscular up‐regulation of IL‐18 and IL‐18R suggest that deregulation of the IL‐18/IL‐18R pathway is a pathogenetic mechanism in IM. Measurement of IL‐18 may thus predict the severity of both DM and PM.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Chemokine CCL2 - metabolism</subject><subject>Clinical Studies</subject><subject>dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Dermatomyositis - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunopathology</subject><subject>inflammation</subject><subject>Interferon-gamma - blood</subject><subject>Interleukin-18 - blood</subject><subject>Interleukin-18 - metabolism</subject><subject>Interleukin-4 - blood</subject><subject>Interleukin-6 - blood</subject><subject>interleukin‐18</subject><subject>Macrophages - immunology</subject><subject>Male</subject><subject>MCP‐1</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Muscle, Skeletal - immunology</subject><subject>myositis</subject><subject>Myositis - immunology</subject><subject>Polymyositis - immunology</subject><subject>Receptors, Interleukin-18 - metabolism</subject><subject>Th1 Cells - immunology</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcGO0zAQhi0EYsvCK6AICW4Jdpw49gEkVC1QaSUucLamzoS668TFTkp74xF4Rp4Eh1a7wAV8GY_mm9E_8xOSMVqw9F5uC8ZFnZdlpYqSUlFQziQtDvfI4rZwnywopSpXjFYX5FGM25QKIcqH5IIJJSRX1YKY1TBicDjd2OHHt-9MZn6PAQ-7gDFaP2QQM8g24FwP4SbzXTZuMAMz2r0dj3MO0-ht308DZnboHPQ9jD4cs_7odzBuLMbH5EEHLuKTc7wkn95efVy-z68_vFst31znRpSC5pwCrE0lm7bkddk1ChUTTAGKRrGmrZRoO6Zq060NdpIpqZrWrFvAGltVQccvyevT3N207rE1OIwBnN4Fm7QftQer_6wMdqM_-71Ot1MNl2nAi_OA4L9MGEfd22jQORjQT1ELKeuqLJt_gkkmaypeJ_DZX-DWT2FIV0iMkGkxyRMkT5AJPsaA3a1kRvXst97q2VY926pnv_Uvv_UhtT79feW7xrPBCXh-BiAacF2Awdh4x0lW0orPG706cV-tw-N_C9DLq9X84z8BwHjJ1Q</recordid><startdate>200610</startdate><enddate>200610</enddate><creator>Tucci, M.</creator><creator>Quatraro, C.</creator><creator>Dammacco, F.</creator><creator>Silvestris, F.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><general>Blackwell Science Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200610</creationdate><title>Interleukin‐18 overexpression as a hallmark of the activity of autoimmune inflammatory myopathies</title><author>Tucci, M. ; Quatraro, C. ; Dammacco, F. ; Silvestris, F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6260-30aabc487d2352f79e91619ae67917d496df195cfbcef819897dcbdae5ed94af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Chemokine CCL2 - metabolism</topic><topic>Clinical Studies</topic><topic>dendritic cells</topic><topic>Dendritic Cells - immunology</topic><topic>Dermatomyositis - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunopathology</topic><topic>inflammation</topic><topic>Interferon-gamma - blood</topic><topic>Interleukin-18 - blood</topic><topic>Interleukin-18 - metabolism</topic><topic>Interleukin-4 - blood</topic><topic>Interleukin-6 - blood</topic><topic>interleukin‐18</topic><topic>Macrophages - immunology</topic><topic>Male</topic><topic>MCP‐1</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Muscle, Skeletal - immunology</topic><topic>myositis</topic><topic>Myositis - immunology</topic><topic>Polymyositis - immunology</topic><topic>Receptors, Interleukin-18 - metabolism</topic><topic>Th1 Cells - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tucci, M.</creatorcontrib><creatorcontrib>Quatraro, C.</creatorcontrib><creatorcontrib>Dammacco, F.</creatorcontrib><creatorcontrib>Silvestris, F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tucci, M.</au><au>Quatraro, C.</au><au>Dammacco, F.</au><au>Silvestris, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interleukin‐18 overexpression as a hallmark of the activity of autoimmune inflammatory myopathies</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>2006-10</date><risdate>2006</risdate><volume>146</volume><issue>1</issue><spage>21</spage><epage>31</epage><pages>21-31</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>Summary
The objective of this study was to explore the role of interleukin (IL)‐18 in patients with inflammatory myopathies (IM) such as dermatomyositis (DM) and polymyositis (PM) in relation to the possible predominance of a Th1 immune response in their pathogenesis. Serum concentrations of IL‐18, interferon (IFN)‐γ, IL‐4 and IL‐6 were measured in six patients by enzyme‐linked immunosorbent assay (ELISA). IL‐18 expression was evaluated by in situ hybridization (ISH), whereas CD68, CD8 and CD83 were investigated by immunohistochemistry (IHC) to define the main producers of IL‐18. Lastly, the expression of both IL‐18 receptor (IL‐18R) and monocyte chemoattractant protein (MCP)‐1 was also explored by IHC. High serum levels of IL‐18 and IFN‐γ, and conversely low titres of IL‐4 and IL‐6, were demonstrated in both diseases. In addition, IL‐18 was overexpressed in muscle biopsy specimens from patients with IM. Both macrophages and dendritic cells (DC) surrounding either perivascular and perimysium areas in DM or endomysium in PM were the main producers of IL‐18. Endothelial cells (EC), smooth muscle cells (SMC) and CD8+ T cells expressed a high content of IL‐18R. Vessel cells overexpressed MCP‐1 in parallel with IL‐18R. High concentrations of serum IL‐18 as well as muscular up‐regulation of IL‐18 and IL‐18R suggest that deregulation of the IL‐18/IL‐18R pathway is a pathogenetic mechanism in IM. Measurement of IL‐18 may thus predict the severity of both DM and PM.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16968394</pmid><doi>10.1111/j.1365-2249.2006.03180.x</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adult Biological and medical sciences CD8-Positive T-Lymphocytes - immunology Chemokine CCL2 - metabolism Clinical Studies dendritic cells Dendritic Cells - immunology Dermatomyositis - immunology Enzyme-Linked Immunosorbent Assay - methods Female Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunopathology inflammation Interferon-gamma - blood Interleukin-18 - blood Interleukin-18 - metabolism Interleukin-4 - blood Interleukin-6 - blood interleukin‐18 Macrophages - immunology Male MCP‐1 Medical sciences Middle Aged Muscle, Skeletal - immunology myositis Myositis - immunology Polymyositis - immunology Receptors, Interleukin-18 - metabolism Th1 Cells - immunology |
| title | Interleukin‐18 overexpression as a hallmark of the activity of autoimmune inflammatory myopathies |
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