Role of interleukin (IL‐10) in probiotic‐mediated immune modulation: an assessment in wild‐type and IL‐10 knock‐out mice

Summary While the impact of Bifidobacterium infantis 35624 and other probiotics on cytokines has been shown in established colitis, the effects of B. infantis consumption in pre‐inflammation of interleukin (IL)‐10 knock‐out (KO) mice and on the wild‐type (WT) C57Bl/6 mice have not been well demonstr...

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Veröffentlicht in:Clinical and experimental immunology 2006-05, Vol.144 (2), p.273-280
Hauptverfasser: Sheil, B., MacSharry, J., O'Callaghan, L., O'Riordan, A., Waters, A., Morgan, J., Collins, J. K., O'Mahony, L., Shanahan, F.
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container_issue 2
container_start_page 273
container_title Clinical and experimental immunology
container_volume 144
creator Sheil, B.
MacSharry, J.
O'Callaghan, L.
O'Riordan, A.
Waters, A.
Morgan, J.
Collins, J. K.
O'Mahony, L.
Shanahan, F.
description Summary While the impact of Bifidobacterium infantis 35624 and other probiotics on cytokines has been shown in established colitis, the effects of B. infantis consumption in pre‐inflammation of interleukin (IL)‐10 knock‐out (KO) mice and on the wild‐type (WT) C57Bl/6 mice have not been well demonstrated. The objective of this study was to examine cytokine responses in mucosal and systemic lymphoid compartments of IL‐10 KO mice early in disease and to compare with control WT mice. Mice were fed B. infantis or placebo for 5 weeks and culled prior to the onset of chronic intestinal inflammation (12–14 weeks). The spleen, Peyer's patches and intestinal mucosa were removed and stimulated with various bacterial stimuli. Cytokine levels were measured by enzyme‐linked immunosorbent assay. While basal intestinal and systemic cytokine profiles of WT and IL‐10 KO mice were similar, transforming growth factor (TGF)‐β was reduced in the spleen of IL‐10 KO mice. Following probiotic consumption, interferon (IFN)‐γ was reduced in the Peyer's patch of both WT and IL‐10 KO mice. Alterations in IFN‐γ in the Peyer's patches of WT mice (enhancement) versus IL‐10 KO (reduction) were observed following in vitro stimulation with salmonella. Differential IL‐12p40, CCL2 and CCL5 responses were also observed in IL‐10 KO mice and WT mice. The cytokine profile of IL‐10 KO mice in early disease was similar to that of WT mice. The most pronounced changes occurred in the Peyer's patch of IL‐10 KO mice, suggesting a probiotic mechanism of action independent of IL‐10. This study provides a rationale for the use of B. infantis 35624 for the treatment of gastrointestinal inflammation.
doi_str_mv 10.1111/j.1365-2249.2006.03051.x
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K. ; O'Mahony, L. ; Shanahan, F.</creator><creatorcontrib>Sheil, B. ; MacSharry, J. ; O'Callaghan, L. ; O'Riordan, A. ; Waters, A. ; Morgan, J. ; Collins, J. K. ; O'Mahony, L. ; Shanahan, F.</creatorcontrib><description>Summary While the impact of Bifidobacterium infantis 35624 and other probiotics on cytokines has been shown in established colitis, the effects of B. infantis consumption in pre‐inflammation of interleukin (IL)‐10 knock‐out (KO) mice and on the wild‐type (WT) C57Bl/6 mice have not been well demonstrated. The objective of this study was to examine cytokine responses in mucosal and systemic lymphoid compartments of IL‐10 KO mice early in disease and to compare with control WT mice. Mice were fed B. infantis or placebo for 5 weeks and culled prior to the onset of chronic intestinal inflammation (12–14 weeks). The spleen, Peyer's patches and intestinal mucosa were removed and stimulated with various bacterial stimuli. Cytokine levels were measured by enzyme‐linked immunosorbent assay. While basal intestinal and systemic cytokine profiles of WT and IL‐10 KO mice were similar, transforming growth factor (TGF)‐β was reduced in the spleen of IL‐10 KO mice. Following probiotic consumption, interferon (IFN)‐γ was reduced in the Peyer's patch of both WT and IL‐10 KO mice. Alterations in IFN‐γ in the Peyer's patches of WT mice (enhancement) versus IL‐10 KO (reduction) were observed following in vitro stimulation with salmonella. Differential IL‐12p40, CCL2 and CCL5 responses were also observed in IL‐10 KO mice and WT mice. The cytokine profile of IL‐10 KO mice in early disease was similar to that of WT mice. The most pronounced changes occurred in the Peyer's patch of IL‐10 KO mice, suggesting a probiotic mechanism of action independent of IL‐10. 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K.</creatorcontrib><creatorcontrib>O'Mahony, L.</creatorcontrib><creatorcontrib>Shanahan, F.</creatorcontrib><title>Role of interleukin (IL‐10) in probiotic‐mediated immune modulation: an assessment in wild‐type and IL‐10 knock‐out mice</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>Summary While the impact of Bifidobacterium infantis 35624 and other probiotics on cytokines has been shown in established colitis, the effects of B. infantis consumption in pre‐inflammation of interleukin (IL)‐10 knock‐out (KO) mice and on the wild‐type (WT) C57Bl/6 mice have not been well demonstrated. The objective of this study was to examine cytokine responses in mucosal and systemic lymphoid compartments of IL‐10 KO mice early in disease and to compare with control WT mice. Mice were fed B. infantis or placebo for 5 weeks and culled prior to the onset of chronic intestinal inflammation (12–14 weeks). 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Psychology</subject><subject>Fundamental immunology</subject><subject>Immunopathology</subject><subject>Interferon-gamma - immunology</subject><subject>Interleukin-10 - immunology</subject><subject>Intestinal Mucosa - immunology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Knockout</subject><subject>Peyer's Patches - immunology</subject><subject>Probiotics</subject><subject>Salmonella</subject><subject>Spleen - immunology</subject><subject>Transforming Growth Factor beta - immunology</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks2KFDEQx4Mo7rj6ChIERQ_dJp2PTgQXZFh1YEAQPYd0Oq2Z6U5mk2535yY-gc_ok5h2hl31ormkUvX7F1WpAgBiVOJ8nm9KTDgrqorKskKIl4gghsurW2BxHbgNFgghWUiM6Am4l9ImPznn1V1wgjknVCC8AN_eh97C0EHnRxt7O22dh09X6x9fv2P0LHvhLobGhdGZ7Bps6_RoW-iGYfIWDqGdej264F9A7aFOyaY0WD_OwkvXt1kz7nc2B1t4TAq3PphtNsM0wsEZex_c6XSf7IPjfQo-vj7_sHxbrN-9WS1frQvDK46Lumt4Q4mk1DJTSdqw7CDYti1tSK0l72orukpjIkxDc1hiYQwijMiG4kqQU3B2yLubmtyIyWVG3atddIOOexW0U39GvPusPoUvCgskOa9zgifHBDFcTDaNanDJ2L7X3oYpKV4LSRmW_wSxFFQyOoOP_gI3YYo-_0JmuGCCIpYhcYBMDClF212XjJGa10Ft1Dx1NU9dzeugfq2DusrSh7-3fCM8zj8Dj4-ATkb3XdTeuHTD1bxmnJDMvTxweah2_98FqOX5arbIT2Wo1Xc</recordid><startdate>200605</startdate><enddate>200605</enddate><creator>Sheil, B.</creator><creator>MacSharry, J.</creator><creator>O'Callaghan, L.</creator><creator>O'Riordan, A.</creator><creator>Waters, A.</creator><creator>Morgan, J.</creator><creator>Collins, J. K.</creator><creator>O'Mahony, L.</creator><creator>Shanahan, F.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><general>Blackwell Science Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>M7N</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200605</creationdate><title>Role of interleukin (IL‐10) in probiotic‐mediated immune modulation: an assessment in wild‐type and IL‐10 knock‐out mice</title><author>Sheil, B. ; MacSharry, J. ; O'Callaghan, L. ; O'Riordan, A. ; Waters, A. ; Morgan, J. ; Collins, J. 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K.</au><au>O'Mahony, L.</au><au>Shanahan, F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Role of interleukin (IL‐10) in probiotic‐mediated immune modulation: an assessment in wild‐type and IL‐10 knock‐out mice</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>2006-05</date><risdate>2006</risdate><volume>144</volume><issue>2</issue><spage>273</spage><epage>280</epage><pages>273-280</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>Summary While the impact of Bifidobacterium infantis 35624 and other probiotics on cytokines has been shown in established colitis, the effects of B. infantis consumption in pre‐inflammation of interleukin (IL)‐10 knock‐out (KO) mice and on the wild‐type (WT) C57Bl/6 mice have not been well demonstrated. The objective of this study was to examine cytokine responses in mucosal and systemic lymphoid compartments of IL‐10 KO mice early in disease and to compare with control WT mice. Mice were fed B. infantis or placebo for 5 weeks and culled prior to the onset of chronic intestinal inflammation (12–14 weeks). The spleen, Peyer's patches and intestinal mucosa were removed and stimulated with various bacterial stimuli. Cytokine levels were measured by enzyme‐linked immunosorbent assay. While basal intestinal and systemic cytokine profiles of WT and IL‐10 KO mice were similar, transforming growth factor (TGF)‐β was reduced in the spleen of IL‐10 KO mice. Following probiotic consumption, interferon (IFN)‐γ was reduced in the Peyer's patch of both WT and IL‐10 KO mice. Alterations in IFN‐γ in the Peyer's patches of WT mice (enhancement) versus IL‐10 KO (reduction) were observed following in vitro stimulation with salmonella. Differential IL‐12p40, CCL2 and CCL5 responses were also observed in IL‐10 KO mice and WT mice. The cytokine profile of IL‐10 KO mice in early disease was similar to that of WT mice. The most pronounced changes occurred in the Peyer's patch of IL‐10 KO mice, suggesting a probiotic mechanism of action independent of IL‐10. This study provides a rationale for the use of B. infantis 35624 for the treatment of gastrointestinal inflammation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16634801</pmid><doi>10.1111/j.1365-2249.2006.03051.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record>
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source Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection
subjects Animal Studies
Animals
Bifidobacterium - immunology
Bifidobacterium infantis
Biological and medical sciences
colitis
Colitis - immunology
cytokines
Cytokines - immunology
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Immunopathology
Interferon-gamma - immunology
Interleukin-10 - immunology
Intestinal Mucosa - immunology
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Knockout
Peyer's Patches - immunology
Probiotics
Salmonella
Spleen - immunology
Transforming Growth Factor beta - immunology
title Role of interleukin (IL‐10) in probiotic‐mediated immune modulation: an assessment in wild‐type and IL‐10 knock‐out mice
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