The presence of antibodies to oxidative modified proteins in serum from polycystic ovary syndrome patients

Summary Polycystic ovary syndrome (PCOS) affects 5–10% of women of reproductive age. Free radicals, as a product of oxidative stress, impair cells and tissue properties related to human fertility. These free radicals, together with the oxidized molecules, may have a cytotoxic or deleterious effects...

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Veröffentlicht in:Clinical and experimental immunology 2006-05, Vol.144 (2), p.217-222
Hauptverfasser: Palacio, J. R., Iborra, A., Ulcova‐Gallova, Z., Badia, R., Martínez, P.
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container_start_page 217
container_title Clinical and experimental immunology
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creator Palacio, J. R.
Iborra, A.
Ulcova‐Gallova, Z.
Badia, R.
Martínez, P.
description Summary Polycystic ovary syndrome (PCOS) affects 5–10% of women of reproductive age. Free radicals, as a product of oxidative stress, impair cells and tissue properties related to human fertility. These free radicals, together with the oxidized molecules, may have a cytotoxic or deleterious effects on sperm and oocytes, on early embryo development or on the endometrium. Aldehyde‐modified proteins are highly immunogenic and circulating autoantibodies to new epitopes, such as malondialdehyde (MDA), may affect the reproductive system. Autoantibodies or elevated reactive oxygen species (ROS) in serum are often associated with inflammatory response. The purpose of this work is to investigate whether PCOS women show increased levels of oxidized proteins (protein–MDA) and anti‐endometrial antibodies (AEA) in their sera, compared with control patients, and to determine whether AEA specificity is related to oxidized protein derivatives. Sera from 31 women [10 patients with PCOS (PCOS group) and 21 women with male factor of infertility (control group)] were chosen from patients attending for infertility. Anti‐endometrial antibodies were determined by enzyme‐linked immunosorbent assay (ELISA) with an endometrial cell line (RL‐95). Antibodies against MDA modified human serum albumin (HSA–MDA) were also determined by ELISA. Oxidized proteins (protein–MDA) in serum were determined by a colorimetric assay. Patients with PCOS have significantly higher levels of AEA and anti‐HSA–MDA, as well as oxidized proteins (protein–MDA) in serum than control patients. For the first time, we describe an autoimmune response in PCOS patients, in terms of AEA. The evidence of protein–MDA in the serum of these patients, together with the increased antibody reactivity to MDA‐modified proteins (HSA–MDA) in vitro, supports the conclusion that oxidative stress may be one of the important causes for abnormal endometrial environment with poor embryo receptivity in PCOS patients.
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The purpose of this work is to investigate whether PCOS women show increased levels of oxidized proteins (protein–MDA) and anti‐endometrial antibodies (AEA) in their sera, compared with control patients, and to determine whether AEA specificity is related to oxidized protein derivatives. Sera from 31 women [10 patients with PCOS (PCOS group) and 21 women with male factor of infertility (control group)] were chosen from patients attending for infertility. Anti‐endometrial antibodies were determined by enzyme‐linked immunosorbent assay (ELISA) with an endometrial cell line (RL‐95). Antibodies against MDA modified human serum albumin (HSA–MDA) were also determined by ELISA. Oxidized proteins (protein–MDA) in serum were determined by a colorimetric assay. Patients with PCOS have significantly higher levels of AEA and anti‐HSA–MDA, as well as oxidized proteins (protein–MDA) in serum than control patients. For the first time, we describe an autoimmune response in PCOS patients, in terms of AEA. The evidence of protein–MDA in the serum of these patients, together with the increased antibody reactivity to MDA‐modified proteins (HSA–MDA) in vitro, supports the conclusion that oxidative stress may be one of the important causes for abnormal endometrial environment with poor embryo receptivity in PCOS patients.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>DOI: 10.1111/j.1365-2249.2006.03061.x</identifier><identifier>PMID: 16634794</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Antibody Specificity - immunology ; anti‐endometrial antibodies (AEA) ; Autoantibodies - blood ; autoimmunity ; Biological and medical sciences ; Blood Proteins - analysis ; Cell Line ; Clinical Studies ; Endometrium - immunology ; Epithelium - immunology ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Immunopathology ; malondialdehyde (MDA) ; Malondialdehyde - blood ; Malondialdehyde - immunology ; Medical sciences ; Middle Aged ; Oxidation-Reduction ; oxidative stress ; Oxidative Stress - immunology ; polycystic ovary syndrome (PCOS) ; Polycystic Ovary Syndrome - blood ; Polycystic Ovary Syndrome - immunology ; Serum Albumin - immunology</subject><ispartof>Clinical and experimental immunology, 2006-05, Vol.144 (2), p.217-222</ispartof><rights>2006 INIST-CNRS</rights><rights>Copyright Blackwell Publishing May 2006</rights><rights>2006 British Society for Immunology 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6261-b64bef772f676987878a3640b70473665804086b5194d3fae8f67f9cffde0e093</citedby><cites>FETCH-LOGICAL-c6261-b64bef772f676987878a3640b70473665804086b5194d3fae8f67f9cffde0e093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1809652/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1809652/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=17675626$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16634794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palacio, J. R.</creatorcontrib><creatorcontrib>Iborra, A.</creatorcontrib><creatorcontrib>Ulcova‐Gallova, Z.</creatorcontrib><creatorcontrib>Badia, R.</creatorcontrib><creatorcontrib>Martínez, P.</creatorcontrib><title>The presence of antibodies to oxidative modified proteins in serum from polycystic ovary syndrome patients</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>Summary Polycystic ovary syndrome (PCOS) affects 5–10% of women of reproductive age. Free radicals, as a product of oxidative stress, impair cells and tissue properties related to human fertility. These free radicals, together with the oxidized molecules, may have a cytotoxic or deleterious effects on sperm and oocytes, on early embryo development or on the endometrium. Aldehyde‐modified proteins are highly immunogenic and circulating autoantibodies to new epitopes, such as malondialdehyde (MDA), may affect the reproductive system. Autoantibodies or elevated reactive oxygen species (ROS) in serum are often associated with inflammatory response. The purpose of this work is to investigate whether PCOS women show increased levels of oxidized proteins (protein–MDA) and anti‐endometrial antibodies (AEA) in their sera, compared with control patients, and to determine whether AEA specificity is related to oxidized protein derivatives. Sera from 31 women [10 patients with PCOS (PCOS group) and 21 women with male factor of infertility (control group)] were chosen from patients attending for infertility. Anti‐endometrial antibodies were determined by enzyme‐linked immunosorbent assay (ELISA) with an endometrial cell line (RL‐95). Antibodies against MDA modified human serum albumin (HSA–MDA) were also determined by ELISA. Oxidized proteins (protein–MDA) in serum were determined by a colorimetric assay. 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The evidence of protein–MDA in the serum of these patients, together with the increased antibody reactivity to MDA‐modified proteins (HSA–MDA) in vitro, supports the conclusion that oxidative stress may be one of the important causes for abnormal endometrial environment with poor embryo receptivity in PCOS patients.</description><subject>Adult</subject><subject>Antibody Specificity - immunology</subject><subject>anti‐endometrial antibodies (AEA)</subject><subject>Autoantibodies - blood</subject><subject>autoimmunity</subject><subject>Biological and medical sciences</subject><subject>Blood Proteins - analysis</subject><subject>Cell Line</subject><subject>Clinical Studies</subject><subject>Endometrium - immunology</subject><subject>Epithelium - immunology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. 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R.</au><au>Iborra, A.</au><au>Ulcova‐Gallova, Z.</au><au>Badia, R.</au><au>Martínez, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The presence of antibodies to oxidative modified proteins in serum from polycystic ovary syndrome patients</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>2006-05</date><risdate>2006</risdate><volume>144</volume><issue>2</issue><spage>217</spage><epage>222</epage><pages>217-222</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>Summary Polycystic ovary syndrome (PCOS) affects 5–10% of women of reproductive age. Free radicals, as a product of oxidative stress, impair cells and tissue properties related to human fertility. These free radicals, together with the oxidized molecules, may have a cytotoxic or deleterious effects on sperm and oocytes, on early embryo development or on the endometrium. Aldehyde‐modified proteins are highly immunogenic and circulating autoantibodies to new epitopes, such as malondialdehyde (MDA), may affect the reproductive system. Autoantibodies or elevated reactive oxygen species (ROS) in serum are often associated with inflammatory response. The purpose of this work is to investigate whether PCOS women show increased levels of oxidized proteins (protein–MDA) and anti‐endometrial antibodies (AEA) in their sera, compared with control patients, and to determine whether AEA specificity is related to oxidized protein derivatives. Sera from 31 women [10 patients with PCOS (PCOS group) and 21 women with male factor of infertility (control group)] were chosen from patients attending for infertility. Anti‐endometrial antibodies were determined by enzyme‐linked immunosorbent assay (ELISA) with an endometrial cell line (RL‐95). Antibodies against MDA modified human serum albumin (HSA–MDA) were also determined by ELISA. Oxidized proteins (protein–MDA) in serum were determined by a colorimetric assay. Patients with PCOS have significantly higher levels of AEA and anti‐HSA–MDA, as well as oxidized proteins (protein–MDA) in serum than control patients. For the first time, we describe an autoimmune response in PCOS patients, in terms of AEA. The evidence of protein–MDA in the serum of these patients, together with the increased antibody reactivity to MDA‐modified proteins (HSA–MDA) in vitro, supports the conclusion that oxidative stress may be one of the important causes for abnormal endometrial environment with poor embryo receptivity in PCOS patients.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>16634794</pmid><doi>10.1111/j.1365-2249.2006.03061.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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subjects Adult
Antibody Specificity - immunology
anti‐endometrial antibodies (AEA)
Autoantibodies - blood
autoimmunity
Biological and medical sciences
Blood Proteins - analysis
Cell Line
Clinical Studies
Endometrium - immunology
Epithelium - immunology
Female
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunopathology
malondialdehyde (MDA)
Malondialdehyde - blood
Malondialdehyde - immunology
Medical sciences
Middle Aged
Oxidation-Reduction
oxidative stress
Oxidative Stress - immunology
polycystic ovary syndrome (PCOS)
Polycystic Ovary Syndrome - blood
Polycystic Ovary Syndrome - immunology
Serum Albumin - immunology
title The presence of antibodies to oxidative modified proteins in serum from polycystic ovary syndrome patients
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