Transcription factor distribution in Escherichia coli: studies with FNR protein
Using chromatin immunoprecipitation (ChIP) and high-density microarrays, we have measured the distribution of the global transcription regulator protein, FNR, across the entire Escherichia coli chromosome in exponentially growing cells. Sixty-three binding targets, each located at the 5′ end of a ge...
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| Veröffentlicht in: | Nucleic acids research 2007-01, Vol.35 (1), p.269-278 |
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| creator | Grainger, David C Aiba, Hirofumi Hurd, Douglas Browning, Douglas F Busby, Stephen J.W |
| description | Using chromatin immunoprecipitation (ChIP) and high-density microarrays, we have measured the distribution of the global transcription regulator protein, FNR, across the entire Escherichia coli chromosome in exponentially growing cells. Sixty-three binding targets, each located at the 5′ end of a gene, were identified. Some targets are adjacent to poorly transcribed genes where FNR has little impact on transcription. In stationary phase, the distribution of FNR was largely unchanged. Control experiments showed that, like FNR, the distribution of the nucleoid-associated protein, IHF, is little altered when cells enter stationary phase, whilst RNA polymerase undergoes a complete redistribution. |
| doi_str_mv | 10.1093/nar/gkl1023 |
| format | Article |
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Control experiments showed that, like FNR, the distribution of the nucleoid-associated protein, IHF, is little altered when cells enter stationary phase, whilst RNA polymerase undergoes a complete redistribution.</description><subject>Binding Sites</subject><subject>Chromatin Immunoprecipitation</subject><subject>Chromosomes, Bacterial</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>DNA, Bacterial - metabolism</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - growth & development</subject><subject>Escherichia coli Proteins - analysis</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Iron-Sulfur Proteins - analysis</subject><subject>Iron-Sulfur Proteins - metabolism</subject><subject>Molecular Biology</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Regulatory Elements, Transcriptional</subject><subject>Sequence Analysis, DNA</subject><subject>Transcription Factors - analysis</subject><subject>Transcription Factors - metabolism</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0Utv1DAUBWALgehQWLGHrNigUF-_4rBAQlU7BVW0QCuhbqw7jjNjmomDnbTw7zHMqIXVrCxdfzp-HEKeA30DtOYHPcaD5XUHlPEHZAZcsVLUij0kM8qpLIEKvUeepPSdUhAgxWOyBxUowXQ1I2cXEftkox9GH_qiRTuGWDQ-jdEvpr8z3xdHya5c9HblsbCh82-LNE6Nd6m49eOqOP70pRhiGJ3vn5JHLXbJPduu--Ty-Oji8KQ8PZt_OHx_WlrJxVhKrtE5DY1AW1FGJQcnUIIELhTqVnHRMNriokEm7AIl2qbSCvME2rau-T55t8kdpsXaNdb1Y8TODNGvMf4yAb35f6f3K7MMNwY0ZVLqHPBqGxDDj8ml0ax9sq7rsHdhSkbpGur8dzshA8Y1KLUTQi0FZTXL8PUG2hhSiq69uzZQ86dSkys120qzfvHvS-_ttsP7c8M07EgqNzC3637eUYzXRlW8kubk25U5vzoXH-f0s5ln_3LjWwwGl9Enc_mVUeCUZs5FxX8DH7LEdQ</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>Grainger, David C</creator><creator>Aiba, Hirofumi</creator><creator>Hurd, Douglas</creator><creator>Browning, Douglas F</creator><creator>Busby, Stephen J.W</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>C1K</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070101</creationdate><title>Transcription factor distribution in Escherichia coli: studies with FNR protein</title><author>Grainger, David C ; 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| subjects | Binding Sites Chromatin Immunoprecipitation Chromosomes, Bacterial DNA, Bacterial - chemistry DNA, Bacterial - isolation & purification DNA, Bacterial - metabolism Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli Proteins - analysis Escherichia coli Proteins - metabolism Iron-Sulfur Proteins - analysis Iron-Sulfur Proteins - metabolism Molecular Biology Oligonucleotide Array Sequence Analysis Regulatory Elements, Transcriptional Sequence Analysis, DNA Transcription Factors - analysis Transcription Factors - metabolism |
| title | Transcription factor distribution in Escherichia coli: studies with FNR protein |
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