CD4+ CCR5+ and CD4+ CCR3+ lymphocyte subset and monocyte apoptosis in patients with acute visceral leishmaniasis
Summary The potential involvement of apoptosis in the pathogenesis of visceral leishmaniasis (VL) was examined by studying spontaneous and Leishmania antigen (LAg)‐induced apoptosis using cryopreserved peripheral blood mononuclear cells (PBMC) of Sicilian patients with VL. Results indicate that mono...
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| Veröffentlicht in: | Immunology 2004-10, Vol.113 (2), p.260-268 |
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| creator | Potestio, Marcella D'Agostino, Pietro Romano, Giuseppina Colonna Milano, Salvatore Ferlazzo, Viviana Aquino, Alessandra Di Bella, Gloria Caruso, Rosalba Gambino, Giuseppe Vitale, Giustina Mansueto, Serafino Cillari, Enrico |
| description | Summary
The potential involvement of apoptosis in the pathogenesis of visceral leishmaniasis (VL) was examined by studying spontaneous and Leishmania antigen (LAg)‐induced apoptosis using cryopreserved peripheral blood mononuclear cells (PBMC) of Sicilian patients with VL. Results indicate that monocytes and T lymphocytes from acute VL patients show a significantly higher level of apoptosis compared with that observed in healed subjects. The percentage of apoptotic cells was higher in monocytes than in T lymphocytes. T cells involved in programmed cell death (PCD) were mainly of the CD4+ phenotype. In particular, the T helper 1‐type (Th1) subset, as evaluated by chemokine receptor‐5 (CCR5) expression, is involved in this process. Cell death in Th1‐type uses a CD95‐mediated mechanism. Furthermore, Th1‐type CCR5+ cells are prone to cell suicide in an autocrine or paracrine way, as attested by enhanced expression of CD95L in acute VL patients. The reduction in Th1‐type cells by apoptosis was confirmed by the decrease in interferon‐γ secretion. In conclusion, apoptosis of monocytes, CD4+ and CD4+ CCR5+ T cells could be involved in the failure of cell mediated immunity that is responsible for severe immune‐depression in VL. |
| doi_str_mv | 10.1111/j.1365-2567.2004.01948.x |
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The potential involvement of apoptosis in the pathogenesis of visceral leishmaniasis (VL) was examined by studying spontaneous and Leishmania antigen (LAg)‐induced apoptosis using cryopreserved peripheral blood mononuclear cells (PBMC) of Sicilian patients with VL. Results indicate that monocytes and T lymphocytes from acute VL patients show a significantly higher level of apoptosis compared with that observed in healed subjects. The percentage of apoptotic cells was higher in monocytes than in T lymphocytes. T cells involved in programmed cell death (PCD) were mainly of the CD4+ phenotype. In particular, the T helper 1‐type (Th1) subset, as evaluated by chemokine receptor‐5 (CCR5) expression, is involved in this process. Cell death in Th1‐type uses a CD95‐mediated mechanism. Furthermore, Th1‐type CCR5+ cells are prone to cell suicide in an autocrine or paracrine way, as attested by enhanced expression of CD95L in acute VL patients. The reduction in Th1‐type cells by apoptosis was confirmed by the decrease in interferon‐γ secretion. In conclusion, apoptosis of monocytes, CD4+ and CD4+ CCR5+ T cells could be involved in the failure of cell mediated immunity that is responsible for severe immune‐depression in VL.</description><identifier>ISSN: 0019-2805</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1111/j.1365-2567.2004.01948.x</identifier><identifier>PMID: 15379987</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Acute Disease ; Adult ; Antigens, Protozoan - immunology ; apoptosis ; Apoptosis - immunology ; CD4+ CCR3 ; CD4+ CCR5 ; CD4-Positive T-Lymphocytes - immunology ; CD8-Positive T-Lymphocytes - immunology ; Cells, Cultured ; fas Receptor - immunology ; Humans ; leishmaniasis ; Leishmaniasis, Visceral - immunology ; Leukocytes, Mononuclear - immunology ; Lymphocyte Subsets - immunology ; monocytes ; Monocytes - immunology ; Original ; Receptors, CCR3 ; Receptors, CCR5 - immunology ; Receptors, Chemokine - immunology ; Th1 Cells - immunology</subject><ispartof>Immunology, 2004-10, Vol.113 (2), p.260-268</ispartof><rights>Copyright Blackwell Scientific Publications Ltd. Oct 2004</rights><rights>2004 Blackwell Publishing Ltd 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4978-a773d910fdb87656c667a1ebf2cc6467bb3e759ed5fe7f952e17a0324abb2ad23</citedby><cites>FETCH-LOGICAL-c4978-a773d910fdb87656c667a1ebf2cc6467bb3e759ed5fe7f952e17a0324abb2ad23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1782561/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1782561/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15379987$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Potestio, Marcella</creatorcontrib><creatorcontrib>D'Agostino, Pietro</creatorcontrib><creatorcontrib>Romano, Giuseppina Colonna</creatorcontrib><creatorcontrib>Milano, Salvatore</creatorcontrib><creatorcontrib>Ferlazzo, Viviana</creatorcontrib><creatorcontrib>Aquino, Alessandra</creatorcontrib><creatorcontrib>Di Bella, Gloria</creatorcontrib><creatorcontrib>Caruso, Rosalba</creatorcontrib><creatorcontrib>Gambino, Giuseppe</creatorcontrib><creatorcontrib>Vitale, Giustina</creatorcontrib><creatorcontrib>Mansueto, Serafino</creatorcontrib><creatorcontrib>Cillari, Enrico</creatorcontrib><title>CD4+ CCR5+ and CD4+ CCR3+ lymphocyte subset and monocyte apoptosis in patients with acute visceral leishmaniasis</title><title>Immunology</title><addtitle>Immunology</addtitle><description>Summary
The potential involvement of apoptosis in the pathogenesis of visceral leishmaniasis (VL) was examined by studying spontaneous and Leishmania antigen (LAg)‐induced apoptosis using cryopreserved peripheral blood mononuclear cells (PBMC) of Sicilian patients with VL. Results indicate that monocytes and T lymphocytes from acute VL patients show a significantly higher level of apoptosis compared with that observed in healed subjects. The percentage of apoptotic cells was higher in monocytes than in T lymphocytes. T cells involved in programmed cell death (PCD) were mainly of the CD4+ phenotype. In particular, the T helper 1‐type (Th1) subset, as evaluated by chemokine receptor‐5 (CCR5) expression, is involved in this process. Cell death in Th1‐type uses a CD95‐mediated mechanism. Furthermore, Th1‐type CCR5+ cells are prone to cell suicide in an autocrine or paracrine way, as attested by enhanced expression of CD95L in acute VL patients. The reduction in Th1‐type cells by apoptosis was confirmed by the decrease in interferon‐γ secretion. In conclusion, apoptosis of monocytes, CD4+ and CD4+ CCR5+ T cells could be involved in the failure of cell mediated immunity that is responsible for severe immune‐depression in VL.</description><subject>Acute Disease</subject><subject>Adult</subject><subject>Antigens, Protozoan - immunology</subject><subject>apoptosis</subject><subject>Apoptosis - immunology</subject><subject>CD4+ CCR3</subject><subject>CD4+ CCR5</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD8-Positive T-Lymphocytes - immunology</subject><subject>Cells, Cultured</subject><subject>fas Receptor - immunology</subject><subject>Humans</subject><subject>leishmaniasis</subject><subject>Leishmaniasis, Visceral - immunology</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Lymphocyte Subsets - immunology</subject><subject>monocytes</subject><subject>Monocytes - immunology</subject><subject>Original</subject><subject>Receptors, CCR3</subject><subject>Receptors, CCR5 - immunology</subject><subject>Receptors, Chemokine - immunology</subject><subject>Th1 Cells - immunology</subject><issn>0019-2805</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu3CAUhlHUKJmmfYUIZdHNyA4XA2aRSpF7i5QoUtSuEcY4w8g2rrGTzK5S37RPUpwZ5dJV2QDnfOfnHH4AIEYpjut0nWLKWUIYFylBKEsRllmePuyBxVPiDVigGE5IjtgheBvCOl4pYuwAHGJGhZS5WICx-JQt__z6XRQ3bAl1V8HnAF3CZtP2K282o4VhKoMdH5HWd9uY7n0_-uACdB3s9ehsNwZ478YV1GaKwJ0Lxg66gY11YdXqzulIvwP7tW6Cfb_bj8CPL5-_F9-Sy-uvF8X5ZWIyKfJEC0EriVFdlbngjBvOhca2rIkxPOOiLKkVTNqK1VbUkhGLhUaUZLosia4IPQIft7r9VLa2MrG72IvqB9fqYaO8dup1pnMrdevvFBZ5_EIcBT7sBAb_c7JhVO08UNPozvopKM5zSTGZXzr5B1z7aejicApLmRFOOItQvoXM4EMYbP3UCUZq9lWt1Wyfmu1Ts6_q0Vf1EEuPX07yXLgzMgJnW-DeNXbz38Lq4upqPtG_QB-0GA</recordid><startdate>200410</startdate><enddate>200410</enddate><creator>Potestio, Marcella</creator><creator>D'Agostino, Pietro</creator><creator>Romano, Giuseppina Colonna</creator><creator>Milano, Salvatore</creator><creator>Ferlazzo, Viviana</creator><creator>Aquino, Alessandra</creator><creator>Di Bella, Gloria</creator><creator>Caruso, Rosalba</creator><creator>Gambino, Giuseppe</creator><creator>Vitale, Giustina</creator><creator>Mansueto, Serafino</creator><creator>Cillari, Enrico</creator><general>Blackwell Science Ltd</general><general>Wiley Subscription Services, Inc</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200410</creationdate><title>CD4+ CCR5+ and CD4+ CCR3+ lymphocyte subset and monocyte apoptosis in patients with acute visceral leishmaniasis</title><author>Potestio, Marcella ; D'Agostino, Pietro ; Romano, Giuseppina Colonna ; Milano, Salvatore ; Ferlazzo, Viviana ; Aquino, Alessandra ; Di Bella, Gloria ; Caruso, Rosalba ; Gambino, Giuseppe ; Vitale, Giustina ; Mansueto, Serafino ; Cillari, Enrico</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4978-a773d910fdb87656c667a1ebf2cc6467bb3e759ed5fe7f952e17a0324abb2ad23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Acute Disease</topic><topic>Adult</topic><topic>Antigens, Protozoan - immunology</topic><topic>apoptosis</topic><topic>Apoptosis - immunology</topic><topic>CD4+ CCR3</topic><topic>CD4+ CCR5</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD8-Positive T-Lymphocytes - immunology</topic><topic>Cells, Cultured</topic><topic>fas Receptor - immunology</topic><topic>Humans</topic><topic>leishmaniasis</topic><topic>Leishmaniasis, Visceral - immunology</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Lymphocyte Subsets - immunology</topic><topic>monocytes</topic><topic>Monocytes - immunology</topic><topic>Original</topic><topic>Receptors, CCR3</topic><topic>Receptors, CCR5 - immunology</topic><topic>Receptors, Chemokine - immunology</topic><topic>Th1 Cells - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Potestio, Marcella</creatorcontrib><creatorcontrib>D'Agostino, Pietro</creatorcontrib><creatorcontrib>Romano, Giuseppina Colonna</creatorcontrib><creatorcontrib>Milano, Salvatore</creatorcontrib><creatorcontrib>Ferlazzo, Viviana</creatorcontrib><creatorcontrib>Aquino, Alessandra</creatorcontrib><creatorcontrib>Di Bella, Gloria</creatorcontrib><creatorcontrib>Caruso, Rosalba</creatorcontrib><creatorcontrib>Gambino, Giuseppe</creatorcontrib><creatorcontrib>Vitale, Giustina</creatorcontrib><creatorcontrib>Mansueto, Serafino</creatorcontrib><creatorcontrib>Cillari, Enrico</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Potestio, Marcella</au><au>D'Agostino, Pietro</au><au>Romano, Giuseppina Colonna</au><au>Milano, Salvatore</au><au>Ferlazzo, Viviana</au><au>Aquino, Alessandra</au><au>Di Bella, Gloria</au><au>Caruso, Rosalba</au><au>Gambino, Giuseppe</au><au>Vitale, Giustina</au><au>Mansueto, Serafino</au><au>Cillari, Enrico</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CD4+ CCR5+ and CD4+ CCR3+ lymphocyte subset and monocyte apoptosis in patients with acute visceral leishmaniasis</atitle><jtitle>Immunology</jtitle><addtitle>Immunology</addtitle><date>2004-10</date><risdate>2004</risdate><volume>113</volume><issue>2</issue><spage>260</spage><epage>268</epage><pages>260-268</pages><issn>0019-2805</issn><eissn>1365-2567</eissn><abstract>Summary
The potential involvement of apoptosis in the pathogenesis of visceral leishmaniasis (VL) was examined by studying spontaneous and Leishmania antigen (LAg)‐induced apoptosis using cryopreserved peripheral blood mononuclear cells (PBMC) of Sicilian patients with VL. Results indicate that monocytes and T lymphocytes from acute VL patients show a significantly higher level of apoptosis compared with that observed in healed subjects. The percentage of apoptotic cells was higher in monocytes than in T lymphocytes. T cells involved in programmed cell death (PCD) were mainly of the CD4+ phenotype. In particular, the T helper 1‐type (Th1) subset, as evaluated by chemokine receptor‐5 (CCR5) expression, is involved in this process. Cell death in Th1‐type uses a CD95‐mediated mechanism. Furthermore, Th1‐type CCR5+ cells are prone to cell suicide in an autocrine or paracrine way, as attested by enhanced expression of CD95L in acute VL patients. The reduction in Th1‐type cells by apoptosis was confirmed by the decrease in interferon‐γ secretion. In conclusion, apoptosis of monocytes, CD4+ and CD4+ CCR5+ T cells could be involved in the failure of cell mediated immunity that is responsible for severe immune‐depression in VL.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15379987</pmid><doi>10.1111/j.1365-2567.2004.01948.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acute Disease Adult Antigens, Protozoan - immunology apoptosis Apoptosis - immunology CD4+ CCR3 CD4+ CCR5 CD4-Positive T-Lymphocytes - immunology CD8-Positive T-Lymphocytes - immunology Cells, Cultured fas Receptor - immunology Humans leishmaniasis Leishmaniasis, Visceral - immunology Leukocytes, Mononuclear - immunology Lymphocyte Subsets - immunology monocytes Monocytes - immunology Original Receptors, CCR3 Receptors, CCR5 - immunology Receptors, Chemokine - immunology Th1 Cells - immunology |
| title | CD4+ CCR5+ and CD4+ CCR3+ lymphocyte subset and monocyte apoptosis in patients with acute visceral leishmaniasis |
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