Intestinal epithelial exosomes carry MHC class II/peptides able to inform the immune system in mice

Background: Intestinal epithelial cells secrete exosome-like vesicles. The aim of this study was to characterise murine intestinal epithelial exosomes and to analyse their capacity to inform the immune system in vivo in mice. Methods: Epithelial exosomes were obtained from the murine epithelial cell...

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Veröffentlicht in:	Gut 2003-12, Vol.52 (12), p.1690-1697
Hauptverfasser:	Van Niel, G, Mallegol, J, Bevilacqua, C, Candalh, C, Brugière, S, Tomaskovic-Crook, E, Heath, J K, Cerf-Bensussan, N, Heyman, M
Format:	Artikel
Sprache:	eng
Schlagworte:	A33 antigen Animals antigen presenting cell antigen transport Antigens APC Biological and medical sciences Blotting, Western Causes of Cell Line CFA Colorectal diseases complete Freund’s adjuvant Cytoplasmic Vesicles - immunology delayed type hypersensitivity Dendritic cells DMEM DTH Dulbecco’s modified Eagle’s medium Enzymes Epithelial Cells - immunology Epithelium exosomes Female food antigen Fundamental and applied biological sciences. Psychology Gastrointestinal diseases Genetic aspects Health aspects Histocompatibility Antigens Class II - immunology hOVA Hypotheses IEC IFN-γ Immune system Immune System - physiology Immunodeficiency Immunoglobulin E - immunology Immunoglobulin G - immunology Interferon gamma interferon γ Interleukin-1 - pharmacology intestinal epithelial cells intestinal epithelium Intestinal Mucosa - immunology Intestine. Mesentery Lymph Nodes Lymphatic system major histocompatibility complex MALDI-TOF-MS matrix assisted laser desorption ionisation-time of flight-mass spectrometry mesenteric lymph nodes Mesentery - immunology MHC Mice Mice, Inbred C3H MLN Molecular weight mucosal immunity Mucosal Immunology OVA ovalbumin ovalbumin hydrolysate PBS Pepsin Peptides phosphate buffered saline Physiological aspects Proteins SDS-PAGE Secretion Small intestine sodium dodecyl sulphate-polyacrylamide gel electrophoresis Trypsin Vertebrates: digestive system
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