Detection of integrins in human cataract lens epithelial cells and two mammalian lens epithelial cell lines
Aim: To compare the incidence of various integrin subunits in human cataract anterior lens epithelial cells (A-LEC) and in two mammalian LEC lines. Methods: Circular sections of anterior capsules with attached LEC were obtained during cataract surgery. Integrin subunits were immunolocalised in these...
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| Veröffentlicht in: | British journal of ophthalmology 2005-11, Vol.89 (11), p.1506-1509 |
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| creator | McLean, S M Mathew, M R K Kelly, J B Murray, S B Bennett, H G B Webb, L A Esakowitz, L McLean, J S |
| description | Aim: To compare the incidence of various integrin subunits in human cataract anterior lens epithelial cells (A-LEC) and in two mammalian LEC lines. Methods: Circular sections of anterior capsules with attached LEC were obtained during cataract surgery. Integrin subunits were immunolocalised in these anterior LEC and in a human and rabbit LEC line, using four monoclonal antibodies specific for subunits α2, α3, and α5, and β subunit 2. Results: All of these subunits were found in at least a proportion A-LEC samples as follows: α2 71%, α3 92%, α5 62%, and β2 24%. The human LEC line was immunoreactive for α2 and α3 only. The rabbit lens epithelial cell line was immunoreactive for α5 but there was no staining for α2, α3, or β2. Conclusion: The A-LEC and mammalian LEC lines showed a similarity in their pattern of integrin expression. As these integrins are receptors for extracellular matrix (ECM) components, they are likely to be associated with the attachment and migration of LECs that precedes capsular opacification. Therefore these cell lines may be useful in the elucidation of mechanisms involved the pathogenesis of capsule opacification. |
| doi_str_mv | 10.1136/bjo.2005.071886 |
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Methods: Circular sections of anterior capsules with attached LEC were obtained during cataract surgery. Integrin subunits were immunolocalised in these anterior LEC and in a human and rabbit LEC line, using four monoclonal antibodies specific for subunits α2, α3, and α5, and β subunit 2. Results: All of these subunits were found in at least a proportion A-LEC samples as follows: α2 71%, α3 92%, α5 62%, and β2 24%. The human LEC line was immunoreactive for α2 and α3 only. The rabbit lens epithelial cell line was immunoreactive for α5 but there was no staining for α2, α3, or β2. Conclusion: The A-LEC and mammalian LEC lines showed a similarity in their pattern of integrin expression. As these integrins are receptors for extracellular matrix (ECM) components, they are likely to be associated with the attachment and migration of LECs that precedes capsular opacification. Therefore these cell lines may be useful in the elucidation of mechanisms involved the pathogenesis of capsule opacification.</description><identifier>ISSN: 0007-1161</identifier><identifier>EISSN: 1468-2079</identifier><identifier>DOI: 10.1136/bjo.2005.071886</identifier><identifier>PMID: 16234462</identifier><identifier>CODEN: BJOPAL</identifier><language>eng</language><publisher>BMA House, Tavistock Square, London, WC1H 9JR: BMJ Publishing Group Ltd</publisher><subject>A-LEC ; ACO ; Animals ; anterior capsule opacification ; anterior lens epithelial cells ; Antigens ; Biological and medical sciences ; capsule opacification ; Cataract - metabolism ; Cataracts ; CD18 Antigens - analysis ; Cell adhesion & migration ; Cell Line ; Collagen ; DAB ; diaminobenzidine tetrachloride ; ECM ; Epithelial Cells - chemistry ; Extracellular matrix ; extracellular matrix proteins ; Eye surgery ; Humans ; Immunoenzyme Techniques ; Integrin alpha Chains - analysis ; integrins ; Integrins - analysis ; Laboratory Science - Scientific Reports ; LEC ; Lens Capsule, Crystalline - chemistry ; Lens diseases ; lens epithelial cells ; Medical sciences ; Miscellaneous ; Ophthalmology ; Pathogenesis ; PBS ; PCO ; phosphate buffered saline ; posterior capsule opacification ; Rabbits ; Signal transduction ; Species Specificity ; Studies</subject><ispartof>British journal of ophthalmology, 2005-11, Vol.89 (11), p.1506-1509</ispartof><rights>Copyright 2005 British Journal of Ophthalmology</rights><rights>2005 INIST-CNRS</rights><rights>Copyright: 2005 Copyright 2005 British Journal of Ophthalmology</rights><rights>Copyright © Copyright 2005 British Journal of Ophthalmology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b588t-e1355a39edb5037c7809d85e18185a97f5b11c3f0695d34d2473401f49bc45763</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1772959/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1772959/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17242114$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16234462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McLean, S M</creatorcontrib><creatorcontrib>Mathew, M R K</creatorcontrib><creatorcontrib>Kelly, J B</creatorcontrib><creatorcontrib>Murray, S B</creatorcontrib><creatorcontrib>Bennett, H G B</creatorcontrib><creatorcontrib>Webb, L A</creatorcontrib><creatorcontrib>Esakowitz, L</creatorcontrib><creatorcontrib>McLean, J S</creatorcontrib><title>Detection of integrins in human cataract lens epithelial cells and two mammalian lens epithelial cell lines</title><title>British journal of ophthalmology</title><addtitle>Br J Ophthalmol</addtitle><description>Aim: To compare the incidence of various integrin subunits in human cataract anterior lens epithelial cells (A-LEC) and in two mammalian LEC lines. Methods: Circular sections of anterior capsules with attached LEC were obtained during cataract surgery. Integrin subunits were immunolocalised in these anterior LEC and in a human and rabbit LEC line, using four monoclonal antibodies specific for subunits α2, α3, and α5, and β subunit 2. Results: All of these subunits were found in at least a proportion A-LEC samples as follows: α2 71%, α3 92%, α5 62%, and β2 24%. The human LEC line was immunoreactive for α2 and α3 only. The rabbit lens epithelial cell line was immunoreactive for α5 but there was no staining for α2, α3, or β2. Conclusion: The A-LEC and mammalian LEC lines showed a similarity in their pattern of integrin expression. As these integrins are receptors for extracellular matrix (ECM) components, they are likely to be associated with the attachment and migration of LECs that precedes capsular opacification. Therefore these cell lines may be useful in the elucidation of mechanisms involved the pathogenesis of capsule opacification.</description><subject>A-LEC</subject><subject>ACO</subject><subject>Animals</subject><subject>anterior capsule opacification</subject><subject>anterior lens epithelial cells</subject><subject>Antigens</subject><subject>Biological and medical sciences</subject><subject>capsule opacification</subject><subject>Cataract - metabolism</subject><subject>Cataracts</subject><subject>CD18 Antigens - analysis</subject><subject>Cell adhesion & migration</subject><subject>Cell Line</subject><subject>Collagen</subject><subject>DAB</subject><subject>diaminobenzidine tetrachloride</subject><subject>ECM</subject><subject>Epithelial Cells - chemistry</subject><subject>Extracellular matrix</subject><subject>extracellular matrix proteins</subject><subject>Eye surgery</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Integrin alpha Chains - analysis</subject><subject>integrins</subject><subject>Integrins - analysis</subject><subject>Laboratory Science - Scientific Reports</subject><subject>LEC</subject><subject>Lens Capsule, Crystalline - chemistry</subject><subject>Lens diseases</subject><subject>lens epithelial cells</subject><subject>Medical sciences</subject><subject>Miscellaneous</subject><subject>Ophthalmology</subject><subject>Pathogenesis</subject><subject>PBS</subject><subject>PCO</subject><subject>phosphate buffered saline</subject><subject>posterior capsule opacification</subject><subject>Rabbits</subject><subject>Signal transduction</subject><subject>Species Specificity</subject><subject>Studies</subject><issn>0007-1161</issn><issn>1468-2079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkUuPFCEUhYnROO3o2p0hMbowqR5uUTxqY6LtOxPN-NoSiqKm6amCFmgf_17a6syoMXEF3Ptxcs89CN0FsgSg_KTbhGVNCFsSAVLya2gBDZdVTUR7HS0IIaIC4HCEbqW0Kc-ag7iJjoDXtGl4vUAXz2y2JrvgcRiw89meR-dTueH1btIeG5111Cbj0Zay3bq8tqPTIzZ2HBPWvsf5W8CTniZd6v6fHB6dt-k2ujHoMdk7h_MYfXrx_OPqVXX67uXr1ZPTqmNS5soCZUzT1vYdI1QYIUnbS2ZBgmS6FQPrAAwdCG9ZT5u-bgRtCAxN25mGCU6P0eNZd7vrJtsb63PUo9pGN-n4QwXt1J8d79bqPHxVIETdsrYIPDwIxPBlZ1NWk0t7H9rbsEuKS0E4_wXe_wvchF30xdxeS7Zl9cAKdTJTJoaUoh0uRwGi9jGqEqPax6jmGMuPe787uOIPuRXgwQHQyehxiNobl644UTc1QFO4auZcyvb7ZV_HC8UFFUy9_bxSb87O2Ien8r2ShX808920-e-UPwHRZMJo</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>McLean, S M</creator><creator>Mathew, M R K</creator><creator>Kelly, J B</creator><creator>Murray, S B</creator><creator>Bennett, H G B</creator><creator>Webb, L A</creator><creator>Esakowitz, L</creator><creator>McLean, J S</creator><general>BMJ Publishing Group Ltd</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><general>Copyright 2005 British Journal of Ophthalmology</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20051101</creationdate><title>Detection of integrins in human cataract lens epithelial cells and two mammalian lens epithelial cell lines</title><author>McLean, S M ; Mathew, M R K ; Kelly, J B ; Murray, S B ; Bennett, H G B ; Webb, L A ; Esakowitz, L ; McLean, J S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b588t-e1355a39edb5037c7809d85e18185a97f5b11c3f0695d34d2473401f49bc45763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>A-LEC</topic><topic>ACO</topic><topic>Animals</topic><topic>anterior capsule opacification</topic><topic>anterior lens epithelial cells</topic><topic>Antigens</topic><topic>Biological and medical sciences</topic><topic>capsule opacification</topic><topic>Cataract - metabolism</topic><topic>Cataracts</topic><topic>CD18 Antigens - analysis</topic><topic>Cell adhesion & migration</topic><topic>Cell Line</topic><topic>Collagen</topic><topic>DAB</topic><topic>diaminobenzidine tetrachloride</topic><topic>ECM</topic><topic>Epithelial Cells - chemistry</topic><topic>Extracellular matrix</topic><topic>extracellular matrix proteins</topic><topic>Eye surgery</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Integrin alpha Chains - analysis</topic><topic>integrins</topic><topic>Integrins - analysis</topic><topic>Laboratory Science - Scientific Reports</topic><topic>LEC</topic><topic>Lens Capsule, Crystalline - chemistry</topic><topic>Lens diseases</topic><topic>lens epithelial cells</topic><topic>Medical sciences</topic><topic>Miscellaneous</topic><topic>Ophthalmology</topic><topic>Pathogenesis</topic><topic>PBS</topic><topic>PCO</topic><topic>phosphate buffered saline</topic><topic>posterior capsule opacification</topic><topic>Rabbits</topic><topic>Signal transduction</topic><topic>Species Specificity</topic><topic>Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McLean, S M</creatorcontrib><creatorcontrib>Mathew, M R K</creatorcontrib><creatorcontrib>Kelly, J B</creatorcontrib><creatorcontrib>Murray, S B</creatorcontrib><creatorcontrib>Bennett, H G B</creatorcontrib><creatorcontrib>Webb, L A</creatorcontrib><creatorcontrib>Esakowitz, L</creatorcontrib><creatorcontrib>McLean, J S</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McLean, S M</au><au>Mathew, M R K</au><au>Kelly, J B</au><au>Murray, S B</au><au>Bennett, H G B</au><au>Webb, L A</au><au>Esakowitz, L</au><au>McLean, J S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of integrins in human cataract lens epithelial cells and two mammalian lens epithelial cell lines</atitle><jtitle>British journal of ophthalmology</jtitle><addtitle>Br J Ophthalmol</addtitle><date>2005-11-01</date><risdate>2005</risdate><volume>89</volume><issue>11</issue><spage>1506</spage><epage>1509</epage><pages>1506-1509</pages><issn>0007-1161</issn><eissn>1468-2079</eissn><coden>BJOPAL</coden><abstract>Aim: To compare the incidence of various integrin subunits in human cataract anterior lens epithelial cells (A-LEC) and in two mammalian LEC lines. Methods: Circular sections of anterior capsules with attached LEC were obtained during cataract surgery. Integrin subunits were immunolocalised in these anterior LEC and in a human and rabbit LEC line, using four monoclonal antibodies specific for subunits α2, α3, and α5, and β subunit 2. Results: All of these subunits were found in at least a proportion A-LEC samples as follows: α2 71%, α3 92%, α5 62%, and β2 24%. The human LEC line was immunoreactive for α2 and α3 only. The rabbit lens epithelial cell line was immunoreactive for α5 but there was no staining for α2, α3, or β2. Conclusion: The A-LEC and mammalian LEC lines showed a similarity in their pattern of integrin expression. As these integrins are receptors for extracellular matrix (ECM) components, they are likely to be associated with the attachment and migration of LECs that precedes capsular opacification. Therefore these cell lines may be useful in the elucidation of mechanisms involved the pathogenesis of capsule opacification.</abstract><cop>BMA House, Tavistock Square, London, WC1H 9JR</cop><pub>BMJ Publishing Group Ltd</pub><pmid>16234462</pmid><doi>10.1136/bjo.2005.071886</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | A-LEC ACO Animals anterior capsule opacification anterior lens epithelial cells Antigens Biological and medical sciences capsule opacification Cataract - metabolism Cataracts CD18 Antigens - analysis Cell adhesion & migration Cell Line Collagen DAB diaminobenzidine tetrachloride ECM Epithelial Cells - chemistry Extracellular matrix extracellular matrix proteins Eye surgery Humans Immunoenzyme Techniques Integrin alpha Chains - analysis integrins Integrins - analysis Laboratory Science - Scientific Reports LEC Lens Capsule, Crystalline - chemistry Lens diseases lens epithelial cells Medical sciences Miscellaneous Ophthalmology Pathogenesis PBS PCO phosphate buffered saline posterior capsule opacification Rabbits Signal transduction Species Specificity Studies |
| title | Detection of integrins in human cataract lens epithelial cells and two mammalian lens epithelial cell lines |
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