DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture
Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation. Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with...
Gespeichert in:
| Veröffentlicht in: | British journal of ophthalmology 2002-11, Vol.86 (11), p.1303-1305 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1305 |
|---|---|
| container_issue | 11 |
| container_start_page | 1303 |
| container_title | British journal of ophthalmology |
| container_volume | 86 |
| creator | Eter, N Spitznas, M |
| description | Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation. Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with a cocktail of enzymes, washed and cultured in MCDB 131 medium. Cells were incubated with 100 μg/ml thalidomide (dissolved in DMSO as recommended). Control cultures were incubated with DMSO alone or without any supplement. Cell proliferation after 24 hours was determined in a counting chamber. Purity of the cultures was controlled by immunohistochemical staining and labelling with low density lipoprotein. Results: Thalidomide dissolved in DMSO inhibited proliferation of choriocapillary endothelial cells compared to control cultures incubated without any supplement. However, DMSO alone suppressed cell proliferation equally well. Conclusion: In cultured bovine choriocapillary cells the inhibiting effect of thalidomide may at least in part be attributed to the solvent DMSO alone. |
| doi_str_mv | 10.1136/bjo.86.11.1303 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1771360</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A94158595</galeid><sourcerecordid>A94158595</sourcerecordid><originalsourceid>FETCH-LOGICAL-b591t-19b0e82171d8e98a6d811fdaa9a48b68d928bcc22b1182c76b68070c13e1fcd03</originalsourceid><addsrcrecordid>eNqFkt-L1DAQx4so3nr66qMUREGwayZt8-NFONYfp56ehz_fQpqm26xpsiat6H9vyi63KgeShyQzn_lmZjJZdhfQEqAkT5qNXzKSzksoUXktW0BFWIER5dezBUKIFgAEjrJbMW7SFROgN7MjwCUjiFeLzDx7--E8H8xgVMyN601jRh9-5brrtBpz3-VjL61p_WBanXuXq94H45XcGmvlDLrWj722RtpcaWvzbfDWdDrI0STcpIjJjlPQt7MbnbRR39nvx9mnF88_rk6Ls_OXr1YnZ0VTcxgL4A3SDAOFlmnOJGkZQNdKyWXFGsJajlmjFMYNAMOKkmRDFCkoNXSqReVx9nSnu52aQbdKuzFIK7bBDClf4aURf3uc6cXa_xBAaeroLPBwLxD890nHUQwmzqVJp_0UBcXAywrTBN7_B9z4KbhU3KzFESCMykQ93lFrabUwrvPpVbXWLrXIeqc7k8wnvIKa1bxOeHEFnlar0x9dxS93vAo-xqC7y0oBiXlERBoRwUg6i3lEUsC9P_tzwPczkYAHe0BGJW0XpFMmHriS06qm5JCpiaP-eemX4ZsgtKS1ePd5Jejpl4v3X9-8FheJf7Tjm2HzvyR_A1Nw4hY</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1779010203</pqid></control><display><type>article</type><title>DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Eter, N ; Spitznas, M</creator><creatorcontrib>Eter, N ; Spitznas, M</creatorcontrib><description>Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation. Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with a cocktail of enzymes, washed and cultured in MCDB 131 medium. Cells were incubated with 100 μg/ml thalidomide (dissolved in DMSO as recommended). Control cultures were incubated with DMSO alone or without any supplement. Cell proliferation after 24 hours was determined in a counting chamber. Purity of the cultures was controlled by immunohistochemical staining and labelling with low density lipoprotein. Results: Thalidomide dissolved in DMSO inhibited proliferation of choriocapillary endothelial cells compared to control cultures incubated without any supplement. However, DMSO alone suppressed cell proliferation equally well. Conclusion: In cultured bovine choriocapillary cells the inhibiting effect of thalidomide may at least in part be attributed to the solvent DMSO alone.</description><identifier>ISSN: 0007-1161</identifier><identifier>EISSN: 1468-2079</identifier><identifier>DOI: 10.1136/bjo.86.11.1303</identifier><identifier>PMID: 12386094</identifier><identifier>CODEN: BJOPAL</identifier><language>eng</language><publisher>BMA House, Tavistock Square, London, WC1H 9JR: BMJ Publishing Group Ltd</publisher><subject>Analysis ; Angiogenesis Inhibitors - pharmacology ; Animals ; Biological and medical sciences ; Blood lipoproteins ; Capillaries - cytology ; Capillaries - drug effects ; Cattle ; Cell culture ; Cell Division - drug effects ; Cell growth ; Cell proliferation ; Cell research ; Cells, Cultured ; choriocapillary endothelial cells ; Choroid - blood supply ; Choroid - cytology ; Choroid - drug effects ; Dimethyl Sulfoxide - pharmacology ; DMSO ; Endothelium ; Endothelium - cytology ; Endothelium - drug effects ; Experiments ; Eye ; Immunoenzyme Techniques ; Laboratory Science ; Lipoproteins ; Macular degeneration ; Medical sciences ; Methods ; Microscopy, Fluorescence ; Ophthalmology ; Pharmacology. Drug treatments ; Physiological aspects ; Proteolipids ; Solution (Chemistry) ; Solvents ; Statistical analysis ; Thalidomide ; Thalidomide - pharmacology ; Vascular endothelial growth factor</subject><ispartof>British journal of ophthalmology, 2002-11, Vol.86 (11), p.1303-1305</ispartof><rights>Copyright 2002 British Journal of Ophthalmology</rights><rights>2002 INIST-CNRS</rights><rights>COPYRIGHT 2002 BMJ Publishing Group Ltd.</rights><rights>Copyright: 2002 Copyright 2002 British Journal of Ophthalmology</rights><rights>Copyright © Copyright 2002 British Journal of Ophthalmology 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b591t-19b0e82171d8e98a6d811fdaa9a48b68d928bcc22b1182c76b68070c13e1fcd03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1771360/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1771360/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13974576$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12386094$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eter, N</creatorcontrib><creatorcontrib>Spitznas, M</creatorcontrib><title>DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture</title><title>British journal of ophthalmology</title><addtitle>Br J Ophthalmol</addtitle><description>Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation. Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with a cocktail of enzymes, washed and cultured in MCDB 131 medium. Cells were incubated with 100 μg/ml thalidomide (dissolved in DMSO as recommended). Control cultures were incubated with DMSO alone or without any supplement. Cell proliferation after 24 hours was determined in a counting chamber. Purity of the cultures was controlled by immunohistochemical staining and labelling with low density lipoprotein. Results: Thalidomide dissolved in DMSO inhibited proliferation of choriocapillary endothelial cells compared to control cultures incubated without any supplement. However, DMSO alone suppressed cell proliferation equally well. Conclusion: In cultured bovine choriocapillary cells the inhibiting effect of thalidomide may at least in part be attributed to the solvent DMSO alone.</description><subject>Analysis</subject><subject>Angiogenesis Inhibitors - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood lipoproteins</subject><subject>Capillaries - cytology</subject><subject>Capillaries - drug effects</subject><subject>Cattle</subject><subject>Cell culture</subject><subject>Cell Division - drug effects</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Cell research</subject><subject>Cells, Cultured</subject><subject>choriocapillary endothelial cells</subject><subject>Choroid - blood supply</subject><subject>Choroid - cytology</subject><subject>Choroid - drug effects</subject><subject>Dimethyl Sulfoxide - pharmacology</subject><subject>DMSO</subject><subject>Endothelium</subject><subject>Endothelium - cytology</subject><subject>Endothelium - drug effects</subject><subject>Experiments</subject><subject>Eye</subject><subject>Immunoenzyme Techniques</subject><subject>Laboratory Science</subject><subject>Lipoproteins</subject><subject>Macular degeneration</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Microscopy, Fluorescence</subject><subject>Ophthalmology</subject><subject>Pharmacology. Drug treatments</subject><subject>Physiological aspects</subject><subject>Proteolipids</subject><subject>Solution (Chemistry)</subject><subject>Solvents</subject><subject>Statistical analysis</subject><subject>Thalidomide</subject><subject>Thalidomide - pharmacology</subject><subject>Vascular endothelial growth factor</subject><issn>0007-1161</issn><issn>1468-2079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqFkt-L1DAQx4so3nr66qMUREGwayZt8-NFONYfp56ehz_fQpqm26xpsiat6H9vyi63KgeShyQzn_lmZjJZdhfQEqAkT5qNXzKSzksoUXktW0BFWIER5dezBUKIFgAEjrJbMW7SFROgN7MjwCUjiFeLzDx7--E8H8xgVMyN601jRh9-5brrtBpz3-VjL61p_WBanXuXq94H45XcGmvlDLrWj722RtpcaWvzbfDWdDrI0STcpIjJjlPQt7MbnbRR39nvx9mnF88_rk6Ls_OXr1YnZ0VTcxgL4A3SDAOFlmnOJGkZQNdKyWXFGsJajlmjFMYNAMOKkmRDFCkoNXSqReVx9nSnu52aQbdKuzFIK7bBDClf4aURf3uc6cXa_xBAaeroLPBwLxD890nHUQwmzqVJp_0UBcXAywrTBN7_B9z4KbhU3KzFESCMykQ93lFrabUwrvPpVbXWLrXIeqc7k8wnvIKa1bxOeHEFnlar0x9dxS93vAo-xqC7y0oBiXlERBoRwUg6i3lEUsC9P_tzwPczkYAHe0BGJW0XpFMmHriS06qm5JCpiaP-eemX4ZsgtKS1ePd5Jejpl4v3X9-8FheJf7Tjm2HzvyR_A1Nw4hY</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Eter, N</creator><creator>Spitznas, M</creator><general>BMJ Publishing Group Ltd</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><general>Copyright 2002 British Journal of Ophthalmology</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20021101</creationdate><title>DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture</title><author>Eter, N ; Spitznas, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b591t-19b0e82171d8e98a6d811fdaa9a48b68d928bcc22b1182c76b68070c13e1fcd03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analysis</topic><topic>Angiogenesis Inhibitors - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood lipoproteins</topic><topic>Capillaries - cytology</topic><topic>Capillaries - drug effects</topic><topic>Cattle</topic><topic>Cell culture</topic><topic>Cell Division - drug effects</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Cell research</topic><topic>Cells, Cultured</topic><topic>choriocapillary endothelial cells</topic><topic>Choroid - blood supply</topic><topic>Choroid - cytology</topic><topic>Choroid - drug effects</topic><topic>Dimethyl Sulfoxide - pharmacology</topic><topic>DMSO</topic><topic>Endothelium</topic><topic>Endothelium - cytology</topic><topic>Endothelium - drug effects</topic><topic>Experiments</topic><topic>Eye</topic><topic>Immunoenzyme Techniques</topic><topic>Laboratory Science</topic><topic>Lipoproteins</topic><topic>Macular degeneration</topic><topic>Medical sciences</topic><topic>Methods</topic><topic>Microscopy, Fluorescence</topic><topic>Ophthalmology</topic><topic>Pharmacology. Drug treatments</topic><topic>Physiological aspects</topic><topic>Proteolipids</topic><topic>Solution (Chemistry)</topic><topic>Solvents</topic><topic>Statistical analysis</topic><topic>Thalidomide</topic><topic>Thalidomide - pharmacology</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eter, N</creatorcontrib><creatorcontrib>Spitznas, M</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eter, N</au><au>Spitznas, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture</atitle><jtitle>British journal of ophthalmology</jtitle><addtitle>Br J Ophthalmol</addtitle><date>2002-11-01</date><risdate>2002</risdate><volume>86</volume><issue>11</issue><spage>1303</spage><epage>1305</epage><pages>1303-1305</pages><issn>0007-1161</issn><eissn>1468-2079</eissn><coden>BJOPAL</coden><abstract>Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation. Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with a cocktail of enzymes, washed and cultured in MCDB 131 medium. Cells were incubated with 100 μg/ml thalidomide (dissolved in DMSO as recommended). Control cultures were incubated with DMSO alone or without any supplement. Cell proliferation after 24 hours was determined in a counting chamber. Purity of the cultures was controlled by immunohistochemical staining and labelling with low density lipoprotein. Results: Thalidomide dissolved in DMSO inhibited proliferation of choriocapillary endothelial cells compared to control cultures incubated without any supplement. However, DMSO alone suppressed cell proliferation equally well. Conclusion: In cultured bovine choriocapillary cells the inhibiting effect of thalidomide may at least in part be attributed to the solvent DMSO alone.</abstract><cop>BMA House, Tavistock Square, London, WC1H 9JR</cop><pub>BMJ Publishing Group Ltd</pub><pmid>12386094</pmid><doi>10.1136/bjo.86.11.1303</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-1161 |
| ispartof | British journal of ophthalmology, 2002-11, Vol.86 (11), p.1303-1305 |
| issn | 0007-1161 1468-2079 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1771360 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Analysis Angiogenesis Inhibitors - pharmacology Animals Biological and medical sciences Blood lipoproteins Capillaries - cytology Capillaries - drug effects Cattle Cell culture Cell Division - drug effects Cell growth Cell proliferation Cell research Cells, Cultured choriocapillary endothelial cells Choroid - blood supply Choroid - cytology Choroid - drug effects Dimethyl Sulfoxide - pharmacology DMSO Endothelium Endothelium - cytology Endothelium - drug effects Experiments Eye Immunoenzyme Techniques Laboratory Science Lipoproteins Macular degeneration Medical sciences Methods Microscopy, Fluorescence Ophthalmology Pharmacology. Drug treatments Physiological aspects Proteolipids Solution (Chemistry) Solvents Statistical analysis Thalidomide Thalidomide - pharmacology Vascular endothelial growth factor |
| title | DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T20%3A22%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DMSO%20mimics%20inhibitory%20effect%20of%20thalidomide%20on%20choriocapillary%20endothelial%20cell%20proliferation%20in%20culture&rft.jtitle=British%20journal%20of%20ophthalmology&rft.au=Eter,%20N&rft.date=2002-11-01&rft.volume=86&rft.issue=11&rft.spage=1303&rft.epage=1305&rft.pages=1303-1305&rft.issn=0007-1161&rft.eissn=1468-2079&rft.coden=BJOPAL&rft_id=info:doi/10.1136/bjo.86.11.1303&rft_dat=%3Cgale_pubme%3EA94158595%3C/gale_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1779010203&rft_id=info:pmid/12386094&rft_galeid=A94158595&rfr_iscdi=true |