DMSO mimics inhibitory effect of thalidomide on choriocapillary endothelial cell proliferation in culture

Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation. Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:British journal of ophthalmology 2002-11, Vol.86 (11), p.1303-1305
Hauptverfasser: Eter, N, Spitznas, M
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Aim: To evaluate the effect of thalidomide on bovine choriocapillary endothelial cell proliferation. Methods: Posterior segments of bovine eyes were incubated with trypsin. After digestion of the tapetum, pieces of Bruch’s membrane with the adherent choriocapillaris were scraped, digested again with a cocktail of enzymes, washed and cultured in MCDB 131 medium. Cells were incubated with 100 μg/ml thalidomide (dissolved in DMSO as recommended). Control cultures were incubated with DMSO alone or without any supplement. Cell proliferation after 24 hours was determined in a counting chamber. Purity of the cultures was controlled by immunohistochemical staining and labelling with low density lipoprotein. Results: Thalidomide dissolved in DMSO inhibited proliferation of choriocapillary endothelial cells compared to control cultures incubated without any supplement. However, DMSO alone suppressed cell proliferation equally well. Conclusion: In cultured bovine choriocapillary cells the inhibiting effect of thalidomide may at least in part be attributed to the solvent DMSO alone.
ISSN:0007-1161
1468-2079
DOI:10.1136/bjo.86.11.1303