Development of a quantitative assay for SARS coronavirus and correlation of GAPDH mRNA with SARS coronavirus in clinical specimens

Aims: To develop a quantitative reverse transcriptase polymerase chain reaction (Q-RT-PCR) for severe acute respiratory syndrome coronavirus (SARS-CoV) detection and explore the potential of using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA as an internal control to exclude false negative...

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Veröffentlicht in:	Journal of clinical pathology 2005-03, Vol.58 (3), p.276-280
Hauptverfasser:	Wong, S C C, Chan, J K C, Lee, K C, Lo, E S F, Tsang, D N C
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - biosynthesis Actins - genetics Adult Aged Biological and medical sciences Biomarkers - metabolism cDNA complementary deoxyribonucleic acid coronavirus CoV False Negative Reactions Female Follow-Up Studies GAPDH Genes glyceraldehyde-3-phosphate dehydrogenase Glyceraldehyde-3-Phosphate Dehydrogenases - biosynthesis Glyceraldehyde-3-Phosphate Dehydrogenases - genetics Hospitals Humans Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Middle Aged Mortality Original Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Q-RT-PCR Quality Control quantitative reverse transcriptase polymerase chain reaction reverse transcriptase polymerase chain reaction Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - genetics RNA, Viral - genetics RT-PCR Sampling techniques SARS SARS Virus - isolation & purification Sensitivity and Specificity severe acute respiratory syndrome Severe Acute Respiratory Syndrome - diagnosis Urine WHO World Health Organisation
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