Alterations of mononuclear inflammatory cells, CD4/CD8+ T cells, interleukin 1β, and tumour necrosis factor α in the bronchoalveolar lavage fluid, peripheral blood, and skin of patients with systemic sclerosis
Background: Systemic sclerosis (SSc) is a multisystem disease with underlying immune mechanisms. Aims: To investigate the clinicopathological characteristics of the lesions; immunological alterations in the bronchoalveolar lavage fluid (BALF), peripheral blood, and skin; and correlations between the...
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| description | Background: Systemic sclerosis (SSc) is a multisystem disease with underlying immune mechanisms. Aims: To investigate the clinicopathological characteristics of the lesions; immunological alterations in the bronchoalveolar lavage fluid (BALF), peripheral blood, and skin; and correlations between the clinicopathological characteristics and immunological alterations in SSc. Materials/Methods: Skin biopsies, BALF, and peripheral blood samples were obtained from 19 patients (18 women, one man) with SSc and six age and sex matched healthy controls (HCs). Mononuclear inflammatory cells (MICs), CD4/CD8 cells, tumour necrosis factor α (TNFα), and interleukin 1β (IL1-1β) concentrations were examined in all samples using histological methods, enzyme linked immunosorbent assay, and immunoperoxidase staining. Results: The mean (SD) age of the patients with SSc was 34.8 (2.6) years. Proteinuria, positive rheumatoid factor, and C reactive protein were seen in 15.8%, 26.3%, and 26.3% of patients, respectively. Compared with HCs, there were significantly higher: total MICs (macrophages, lymphocytes), neutrophils, and eosinophils in BALF, blood, and skin (all p |
| doi_str_mv | 10.1136/jcp.2004.019224 |
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| fullrecord | <record><control><sourceid>istex_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1770564</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ark_67375_NVC_FZ97LWZD_P</sourcerecordid><originalsourceid>FETCH-LOGICAL-b314t-d796742cd21ac3207932d44d3877e48e62c4e623b0618c436e6c99a879874b213</originalsourceid><addsrcrecordid>eNqFkU9v0zAYxiMEYmVw5uoLF9a0_lc7uSBNKQOkCjgMkHaxHMdZ3Tl2ZCeFfiz4ILvwhXDJGOLExZb8Pu_z_OQny54juECIsOVO9QsMIV1AVGJMH2QzRDnOKaLsYTaDEKO85JSdZE9i3EGICEfkcXaCVozzFSln2c9zO-ggB-NdBL4FnXfejcpqGYBxrZVdJwcfDkBpa-McVGu6rNbFGbj882JcMrB6vDEOoNsfcyBdA4ax82MATqvgo4mglSq5gNvvSQ6GrQZ18E5tvbR77W3KsnIvrzVo7WiaOeh1MP02cVlQW--byTQeIxJjn3C1GyL4aoYtiIc46M4oEBP177Sn2aNW2qif3d2n2aeL15fV23zz4c276nyT1wTRIW94yTjFqsFIKoIhLwluKG1IwbmmhWZY0XSQGjJUKEqYZqosZcHLgtMaI3KavZp8-7HudKMSUyIWfTCdDAfhpRH_TpzZimu_F4hzuGI0GSwng-MvxaDb-10ExbFfkfoVx37F1G_aeHEXKaOStg3SKRP_rjFaUASLpMsnnUmf8-1-LsONYJzwlXj_uRIXVyXffLlai49J_3LS193uvxC_AMP7xmo</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Alterations of mononuclear inflammatory cells, CD4/CD8+ T cells, interleukin 1β, and tumour necrosis factor α in the bronchoalveolar lavage fluid, peripheral blood, and skin of patients with systemic sclerosis</title><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Hussein, M R ; Hassan, H I ; Hofny, E R M ; Elkholy, M ; Fatehy, N A ; Abd Elmoniem, A E A ; Ezz El-Din, A M ; Afifi, O A ; Rashed, H G</creator><creatorcontrib>Hussein, M R ; Hassan, H I ; Hofny, E R M ; Elkholy, M ; Fatehy, N A ; Abd Elmoniem, A E A ; Ezz El-Din, A M ; Afifi, O A ; Rashed, H G</creatorcontrib><description><![CDATA[Background: Systemic sclerosis (SSc) is a multisystem disease with underlying immune mechanisms. Aims: To investigate the clinicopathological characteristics of the lesions; immunological alterations in the bronchoalveolar lavage fluid (BALF), peripheral blood, and skin; and correlations between the clinicopathological characteristics and immunological alterations in SSc. Materials/Methods: Skin biopsies, BALF, and peripheral blood samples were obtained from 19 patients (18 women, one man) with SSc and six age and sex matched healthy controls (HCs). Mononuclear inflammatory cells (MICs), CD4/CD8 cells, tumour necrosis factor α (TNFα), and interleukin 1β (IL1-1β) concentrations were examined in all samples using histological methods, enzyme linked immunosorbent assay, and immunoperoxidase staining. Results: The mean (SD) age of the patients with SSc was 34.8 (2.6) years. Proteinuria, positive rheumatoid factor, and C reactive protein were seen in 15.8%, 26.3%, and 26.3% of patients, respectively. Compared with HCs, there were significantly higher: total MICs (macrophages, lymphocytes), neutrophils, and eosinophils in BALF, blood, and skin (all p<0.05); cytokine concentrations in BALF (TNFα, p<0.001; IL-1, p<0.01) and peripheral blood (p<0.01 and p<0.05); and CD8/CD4+ T cells in peripheral blood (p<0.05). Compared with HCs, lesional skin had significantly higher histiocyte cell counts (p<0.05), lower lymphocyte counts (p<0.05), and higher CD4/CD8 ratios (p<0.001). There were significant correlations between cytokine concentrations and CD8+ T cells and forced vital capacity (p<0.001 and p<0.01, respectively). Conclusions: MICs, CD4/CD8+ cells, and cytokines are altered in SSc. These alterations correlated with the underlying disease process and therefore may have pathogenic, modulatory, and potential prognostic roles in SSc.]]></description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.2004.019224</identifier><identifier>PMID: 15677539</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>BAL ; BALF ; Biological and medical sciences ; bronchoalveolar lavage ; bronchoalveolar lavage fluid ; C reactive protein ; CRP ; diffuse systemic sclerosis ; dSSc ; ECG ; electrocardiogram ; forced vital capacity HC ; FVC ; healthy control ; high resolution computerised tomography ; HRCT ; interleukin ; Investigative techniques, diagnostic techniques (general aspects) ; limited systemic sclerosis ; lSSc ; Medical sciences ; MIC ; mononuclear inflammatory cell ; Original ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; PBS ; phosphate buffer saline ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis ; SSc ; systemic sclerosis ; TGFβ ; TNFα ; transforming growth factor β ; tumour necrosis factor α</subject><ispartof>Journal of clinical pathology, 2005-02, Vol.58 (2), p.178-184</ispartof><rights>Copyright 2005 Journal of Clinical Pathology</rights><rights>2005 INIST-CNRS</rights><rights>Copyright © Copyright 2005 Journal of Clinical Pathology 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b314t-d796742cd21ac3207932d44d3877e48e62c4e623b0618c436e6c99a879874b213</citedby><cites>FETCH-LOGICAL-b314t-d796742cd21ac3207932d44d3877e48e62c4e623b0618c436e6c99a879874b213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1770564/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1770564/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16484108$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Hussein, M R</creatorcontrib><creatorcontrib>Hassan, H I</creatorcontrib><creatorcontrib>Hofny, E R M</creatorcontrib><creatorcontrib>Elkholy, M</creatorcontrib><creatorcontrib>Fatehy, N A</creatorcontrib><creatorcontrib>Abd Elmoniem, A E A</creatorcontrib><creatorcontrib>Ezz El-Din, A M</creatorcontrib><creatorcontrib>Afifi, O A</creatorcontrib><creatorcontrib>Rashed, H G</creatorcontrib><title>Alterations of mononuclear inflammatory cells, CD4/CD8+ T cells, interleukin 1β, and tumour necrosis factor α in the bronchoalveolar lavage fluid, peripheral blood, and skin of patients with systemic sclerosis</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description><![CDATA[Background: Systemic sclerosis (SSc) is a multisystem disease with underlying immune mechanisms. Aims: To investigate the clinicopathological characteristics of the lesions; immunological alterations in the bronchoalveolar lavage fluid (BALF), peripheral blood, and skin; and correlations between the clinicopathological characteristics and immunological alterations in SSc. Materials/Methods: Skin biopsies, BALF, and peripheral blood samples were obtained from 19 patients (18 women, one man) with SSc and six age and sex matched healthy controls (HCs). Mononuclear inflammatory cells (MICs), CD4/CD8 cells, tumour necrosis factor α (TNFα), and interleukin 1β (IL1-1β) concentrations were examined in all samples using histological methods, enzyme linked immunosorbent assay, and immunoperoxidase staining. Results: The mean (SD) age of the patients with SSc was 34.8 (2.6) years. Proteinuria, positive rheumatoid factor, and C reactive protein were seen in 15.8%, 26.3%, and 26.3% of patients, respectively. Compared with HCs, there were significantly higher: total MICs (macrophages, lymphocytes), neutrophils, and eosinophils in BALF, blood, and skin (all p<0.05); cytokine concentrations in BALF (TNFα, p<0.001; IL-1, p<0.01) and peripheral blood (p<0.01 and p<0.05); and CD8/CD4+ T cells in peripheral blood (p<0.05). Compared with HCs, lesional skin had significantly higher histiocyte cell counts (p<0.05), lower lymphocyte counts (p<0.05), and higher CD4/CD8 ratios (p<0.001). There were significant correlations between cytokine concentrations and CD8+ T cells and forced vital capacity (p<0.001 and p<0.01, respectively). Conclusions: MICs, CD4/CD8+ cells, and cytokines are altered in SSc. These alterations correlated with the underlying disease process and therefore may have pathogenic, modulatory, and potential prognostic roles in SSc.]]></description><subject>BAL</subject><subject>BALF</subject><subject>Biological and medical sciences</subject><subject>bronchoalveolar lavage</subject><subject>bronchoalveolar lavage fluid</subject><subject>C reactive protein</subject><subject>CRP</subject><subject>diffuse systemic sclerosis</subject><subject>dSSc</subject><subject>ECG</subject><subject>electrocardiogram</subject><subject>forced vital capacity HC</subject><subject>FVC</subject><subject>healthy control</subject><subject>high resolution computerised tomography</subject><subject>HRCT</subject><subject>interleukin</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>limited systemic sclerosis</subject><subject>lSSc</subject><subject>Medical sciences</subject><subject>MIC</subject><subject>mononuclear inflammatory cell</subject><subject>Original</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>PBS</subject><subject>phosphate buffer saline</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><subject>SSc</subject><subject>systemic sclerosis</subject><subject>TGFβ</subject><subject>TNFα</subject><subject>transforming growth factor β</subject><subject>tumour necrosis factor α</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v0zAYxiMEYmVw5uoLF9a0_lc7uSBNKQOkCjgMkHaxHMdZ3Tl2ZCeFfiz4ILvwhXDJGOLExZb8Pu_z_OQny54juECIsOVO9QsMIV1AVGJMH2QzRDnOKaLsYTaDEKO85JSdZE9i3EGICEfkcXaCVozzFSln2c9zO-ggB-NdBL4FnXfejcpqGYBxrZVdJwcfDkBpa-McVGu6rNbFGbj882JcMrB6vDEOoNsfcyBdA4ax82MATqvgo4mglSq5gNvvSQ6GrQZ18E5tvbR77W3KsnIvrzVo7WiaOeh1MP02cVlQW--byTQeIxJjn3C1GyL4aoYtiIc46M4oEBP177Sn2aNW2qif3d2n2aeL15fV23zz4c276nyT1wTRIW94yTjFqsFIKoIhLwluKG1IwbmmhWZY0XSQGjJUKEqYZqosZcHLgtMaI3KavZp8-7HudKMSUyIWfTCdDAfhpRH_TpzZimu_F4hzuGI0GSwng-MvxaDb-10ExbFfkfoVx37F1G_aeHEXKaOStg3SKRP_rjFaUASLpMsnnUmf8-1-LsONYJzwlXj_uRIXVyXffLlai49J_3LS193uvxC_AMP7xmo</recordid><startdate>200502</startdate><enddate>200502</enddate><creator>Hussein, M R</creator><creator>Hassan, H I</creator><creator>Hofny, E R M</creator><creator>Elkholy, M</creator><creator>Fatehy, N A</creator><creator>Abd Elmoniem, A E A</creator><creator>Ezz El-Din, A M</creator><creator>Afifi, O A</creator><creator>Rashed, H G</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ</general><general>Copyright 2005 Journal of Clinical Pathology</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>200502</creationdate><title>Alterations of mononuclear inflammatory cells, CD4/CD8+ T cells, interleukin 1β, and tumour necrosis factor α in the bronchoalveolar lavage fluid, peripheral blood, and skin of patients with systemic sclerosis</title><author>Hussein, M R ; Hassan, H I ; Hofny, E R M ; Elkholy, M ; Fatehy, N A ; Abd Elmoniem, A E A ; Ezz El-Din, A M ; Afifi, O A ; Rashed, H G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b314t-d796742cd21ac3207932d44d3877e48e62c4e623b0618c436e6c99a879874b213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>BAL</topic><topic>BALF</topic><topic>Biological and medical sciences</topic><topic>bronchoalveolar lavage</topic><topic>bronchoalveolar lavage fluid</topic><topic>C reactive protein</topic><topic>CRP</topic><topic>diffuse systemic sclerosis</topic><topic>dSSc</topic><topic>ECG</topic><topic>electrocardiogram</topic><topic>forced vital capacity HC</topic><topic>FVC</topic><topic>healthy control</topic><topic>high resolution computerised tomography</topic><topic>HRCT</topic><topic>interleukin</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>limited systemic sclerosis</topic><topic>lSSc</topic><topic>Medical sciences</topic><topic>MIC</topic><topic>mononuclear inflammatory cell</topic><topic>Original</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>PBS</topic><topic>phosphate buffer saline</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><topic>SSc</topic><topic>systemic sclerosis</topic><topic>TGFβ</topic><topic>TNFα</topic><topic>transforming growth factor β</topic><topic>tumour necrosis factor α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hussein, M R</creatorcontrib><creatorcontrib>Hassan, H I</creatorcontrib><creatorcontrib>Hofny, E R M</creatorcontrib><creatorcontrib>Elkholy, M</creatorcontrib><creatorcontrib>Fatehy, N A</creatorcontrib><creatorcontrib>Abd Elmoniem, A E A</creatorcontrib><creatorcontrib>Ezz El-Din, A M</creatorcontrib><creatorcontrib>Afifi, O A</creatorcontrib><creatorcontrib>Rashed, H G</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hussein, M R</au><au>Hassan, H I</au><au>Hofny, E R M</au><au>Elkholy, M</au><au>Fatehy, N A</au><au>Abd Elmoniem, A E A</au><au>Ezz El-Din, A M</au><au>Afifi, O A</au><au>Rashed, H G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alterations of mononuclear inflammatory cells, CD4/CD8+ T cells, interleukin 1β, and tumour necrosis factor α in the bronchoalveolar lavage fluid, peripheral blood, and skin of patients with systemic sclerosis</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>2005-02</date><risdate>2005</risdate><volume>58</volume><issue>2</issue><spage>178</spage><epage>184</epage><pages>178-184</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract><![CDATA[Background: Systemic sclerosis (SSc) is a multisystem disease with underlying immune mechanisms. Aims: To investigate the clinicopathological characteristics of the lesions; immunological alterations in the bronchoalveolar lavage fluid (BALF), peripheral blood, and skin; and correlations between the clinicopathological characteristics and immunological alterations in SSc. Materials/Methods: Skin biopsies, BALF, and peripheral blood samples were obtained from 19 patients (18 women, one man) with SSc and six age and sex matched healthy controls (HCs). Mononuclear inflammatory cells (MICs), CD4/CD8 cells, tumour necrosis factor α (TNFα), and interleukin 1β (IL1-1β) concentrations were examined in all samples using histological methods, enzyme linked immunosorbent assay, and immunoperoxidase staining. Results: The mean (SD) age of the patients with SSc was 34.8 (2.6) years. Proteinuria, positive rheumatoid factor, and C reactive protein were seen in 15.8%, 26.3%, and 26.3% of patients, respectively. Compared with HCs, there were significantly higher: total MICs (macrophages, lymphocytes), neutrophils, and eosinophils in BALF, blood, and skin (all p<0.05); cytokine concentrations in BALF (TNFα, p<0.001; IL-1, p<0.01) and peripheral blood (p<0.01 and p<0.05); and CD8/CD4+ T cells in peripheral blood (p<0.05). Compared with HCs, lesional skin had significantly higher histiocyte cell counts (p<0.05), lower lymphocyte counts (p<0.05), and higher CD4/CD8 ratios (p<0.001). There were significant correlations between cytokine concentrations and CD8+ T cells and forced vital capacity (p<0.001 and p<0.01, respectively). Conclusions: MICs, CD4/CD8+ cells, and cytokines are altered in SSc. These alterations correlated with the underlying disease process and therefore may have pathogenic, modulatory, and potential prognostic roles in SSc.]]></abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and Association of Clinical Pathologists</pub><pmid>15677539</pmid><doi>10.1136/jcp.2004.019224</doi><tpages>7</tpages></addata></record> |
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| subjects | BAL BALF Biological and medical sciences bronchoalveolar lavage bronchoalveolar lavage fluid C reactive protein CRP diffuse systemic sclerosis dSSc ECG electrocardiogram forced vital capacity HC FVC healthy control high resolution computerised tomography HRCT interleukin Investigative techniques, diagnostic techniques (general aspects) limited systemic sclerosis lSSc Medical sciences MIC mononuclear inflammatory cell Original Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques PBS phosphate buffer saline Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis SSc systemic sclerosis TGFβ TNFα transforming growth factor β tumour necrosis factor α |
| title | Alterations of mononuclear inflammatory cells, CD4/CD8+ T cells, interleukin 1β, and tumour necrosis factor α in the bronchoalveolar lavage fluid, peripheral blood, and skin of patients with systemic sclerosis |
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