Antimicrobial activity of UMFix tissue fixative
Aims: The aim of this study was to determine the antimicrobial effects of UMFix, an alcohol based tissue fixative, on various microorganisms. The UMFix solution was compared with 10% neutral buffered formalin. Methods: Standard methods to determine microorganism colony counts were performed after ex...
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| Veröffentlicht in: | Journal of clinical pathology 2005-01, Vol.58 (1), p.22-25 |
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| container_title | Journal of clinical pathology |
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| creator | Cleary, T J Morales, A R Nadji, M Nassiri, M Vincek, V |
| description | Aims: The aim of this study was to determine the antimicrobial effects of UMFix, an alcohol based tissue fixative, on various microorganisms. The UMFix solution was compared with 10% neutral buffered formalin. Methods: Standard methods to determine microorganism colony counts were performed after exposure of the microorganisms to UMFix and 10% neutral buffered formalin. Results: After a short exposure, UMFix rapidly killed vegetative bacteria, yeasts, moulds, and viruses. Bacterial spores were resistant to killing by UMFix. All organisms were killed by the 10% neutral buffered formalin preparation. Conclusions: UMFix was microbicidal for vegetative bacteria, yeasts, and aspergillus species after a short exposure, although it was not active against spore forming bacillus species. The methanol content of the fixative was responsible for the killing effect of this fixative. No killing was seen when polyethylene glycol was used alone. |
| doi_str_mv | 10.1136/jcp.2004.021618 |
| format | Article |
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The UMFix solution was compared with 10% neutral buffered formalin. Methods: Standard methods to determine microorganism colony counts were performed after exposure of the microorganisms to UMFix and 10% neutral buffered formalin. Results: After a short exposure, UMFix rapidly killed vegetative bacteria, yeasts, moulds, and viruses. Bacterial spores were resistant to killing by UMFix. All organisms were killed by the 10% neutral buffered formalin preparation. Conclusions: UMFix was microbicidal for vegetative bacteria, yeasts, and aspergillus species after a short exposure, although it was not active against spore forming bacillus species. The methanol content of the fixative was responsible for the killing effect of this fixative. No killing was seen when polyethylene glycol was used alone.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.2004.021618</identifier><identifier>PMID: 15623477</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>Anti-Infective Agents - pharmacology ; Bacteria - drug effects ; Bacteria - growth & development ; Biological and medical sciences ; Colony Count, Microbial ; Fixatives - pharmacology ; Formaldehyde - pharmacology ; Fungi - drug effects ; Fungi - growth & development ; herpes simplex virus ; HSV ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; methanol ; Original ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; PCR ; polymerase chain reaction ; Simplexvirus - drug effects ; Simplexvirus - growth & development ; threshold cycle ; tissue fixation ; Tissue Fixation - methods ; UMFix</subject><ispartof>Journal of clinical pathology, 2005-01, Vol.58 (1), p.22-25</ispartof><rights>Copyright 2005 Journal of Clinical Pathology</rights><rights>2005 INIST-CNRS</rights><rights>Copyright: 2005 Copyright 2005 Journal of Clinical Pathology</rights><rights>Copyright © Copyright 2005 Journal of Clinical Pathology 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b522t-2c664924136da18ba34506babb7e5128c39ff458278ca1ef19772581e94d8eba3</citedby><cites>FETCH-LOGICAL-b522t-2c664924136da18ba34506babb7e5128c39ff458278ca1ef19772581e94d8eba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1770532/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1770532/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,4022,27922,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16432884$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15623477$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cleary, T J</creatorcontrib><creatorcontrib>Morales, A R</creatorcontrib><creatorcontrib>Nadji, M</creatorcontrib><creatorcontrib>Nassiri, M</creatorcontrib><creatorcontrib>Vincek, V</creatorcontrib><title>Antimicrobial activity of UMFix tissue fixative</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description>Aims: The aim of this study was to determine the antimicrobial effects of UMFix, an alcohol based tissue fixative, on various microorganisms. The UMFix solution was compared with 10% neutral buffered formalin. Methods: Standard methods to determine microorganism colony counts were performed after exposure of the microorganisms to UMFix and 10% neutral buffered formalin. Results: After a short exposure, UMFix rapidly killed vegetative bacteria, yeasts, moulds, and viruses. Bacterial spores were resistant to killing by UMFix. All organisms were killed by the 10% neutral buffered formalin preparation. Conclusions: UMFix was microbicidal for vegetative bacteria, yeasts, and aspergillus species after a short exposure, although it was not active against spore forming bacillus species. The methanol content of the fixative was responsible for the killing effect of this fixative. No killing was seen when polyethylene glycol was used alone.</description><subject>Anti-Infective Agents - pharmacology</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - growth & development</subject><subject>Biological and medical sciences</subject><subject>Colony Count, Microbial</subject><subject>Fixatives - pharmacology</subject><subject>Formaldehyde - pharmacology</subject><subject>Fungi - drug effects</subject><subject>Fungi - growth & development</subject><subject>herpes simplex virus</subject><subject>HSV</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>methanol</subject><subject>Original</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>PCR</subject><subject>polymerase chain reaction</subject><subject>Simplexvirus - drug effects</subject><subject>Simplexvirus - growth & development</subject><subject>threshold cycle</subject><subject>tissue fixation</subject><subject>Tissue Fixation - methods</subject><subject>UMFix</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUtPGzEUhS1UBCGw7q4aqYJFpUl8_Z5NJRRBAdGyaGHRjeVxPK3TeaT2DAr_vo4mAtpNV5Z8vnt07j0IvQU8A6BivrLrGcGYzTABAWoPTYBJkjNg4g2a4PSbF5KJQ3QU4wpjoBLoAToELghlUk7Q_LztfeNt6Epv6szY3j_6_inrquz-86XfZL2PcXBZ5TcmSe4Y7Vemju5k907R_eXFt8VVfnv36XpxfpuXnJA-J1YIVhCWMi4NqNJQxrEoTVlKx4EoS4uqYlwRqawBV0EhJeEKXMGWyiV8ij6OvuuhbNzSurYPptbr4BsTnnRnvP5baf1P_aN71CAl5pQkg7OdQeh-Dy72uvHRuro2reuGqIWkFBeCJ_D9P-CqG0KblkteCjBhRTr1FM1HKl0qxuCq5yiA9bYKnarQ2yr0WEWaePd6gxd-d_sEnO4AE62pq2Ba6-MLJxglSrHE5SPnY-82z7oJv7ZLSK6_PCy0_H4jv3JM9VXiP4x82az-m_IPAgqsYg</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>Cleary, T J</creator><creator>Morales, A R</creator><creator>Nadji, M</creator><creator>Nassiri, M</creator><creator>Vincek, V</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><general>Copyright 2005 Journal of Clinical Pathology</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200501</creationdate><title>Antimicrobial activity of UMFix tissue fixative</title><author>Cleary, T J ; Morales, A R ; Nadji, M ; Nassiri, M ; Vincek, V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b522t-2c664924136da18ba34506babb7e5128c39ff458278ca1ef19772581e94d8eba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Anti-Infective Agents - pharmacology</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - growth & development</topic><topic>Biological and medical sciences</topic><topic>Colony Count, Microbial</topic><topic>Fixatives - pharmacology</topic><topic>Formaldehyde - pharmacology</topic><topic>Fungi - drug effects</topic><topic>Fungi - growth & development</topic><topic>herpes simplex virus</topic><topic>HSV</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>methanol</topic><topic>Original</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>PCR</topic><topic>polymerase chain reaction</topic><topic>Simplexvirus - drug effects</topic><topic>Simplexvirus - growth & development</topic><topic>threshold cycle</topic><topic>tissue fixation</topic><topic>Tissue Fixation - methods</topic><topic>UMFix</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cleary, T J</creatorcontrib><creatorcontrib>Morales, A R</creatorcontrib><creatorcontrib>Nadji, M</creatorcontrib><creatorcontrib>Nassiri, M</creatorcontrib><creatorcontrib>Vincek, V</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cleary, T J</au><au>Morales, A R</au><au>Nadji, M</au><au>Nassiri, M</au><au>Vincek, V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antimicrobial activity of UMFix tissue fixative</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>2005-01</date><risdate>2005</risdate><volume>58</volume><issue>1</issue><spage>22</spage><epage>25</epage><pages>22-25</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract>Aims: The aim of this study was to determine the antimicrobial effects of UMFix, an alcohol based tissue fixative, on various microorganisms. The UMFix solution was compared with 10% neutral buffered formalin. Methods: Standard methods to determine microorganism colony counts were performed after exposure of the microorganisms to UMFix and 10% neutral buffered formalin. Results: After a short exposure, UMFix rapidly killed vegetative bacteria, yeasts, moulds, and viruses. Bacterial spores were resistant to killing by UMFix. All organisms were killed by the 10% neutral buffered formalin preparation. Conclusions: UMFix was microbicidal for vegetative bacteria, yeasts, and aspergillus species after a short exposure, although it was not active against spore forming bacillus species. The methanol content of the fixative was responsible for the killing effect of this fixative. No killing was seen when polyethylene glycol was used alone.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and Association of Clinical Pathologists</pub><pmid>15623477</pmid><doi>10.1136/jcp.2004.021618</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Infective Agents - pharmacology Bacteria - drug effects Bacteria - growth & development Biological and medical sciences Colony Count, Microbial Fixatives - pharmacology Formaldehyde - pharmacology Fungi - drug effects Fungi - growth & development herpes simplex virus HSV Investigative techniques, diagnostic techniques (general aspects) Medical sciences methanol Original Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques PCR polymerase chain reaction Simplexvirus - drug effects Simplexvirus - growth & development threshold cycle tissue fixation Tissue Fixation - methods UMFix |
| title | Antimicrobial activity of UMFix tissue fixative |
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