Pseudoplatelets: a retrospective study of their incidence and interference with platelet counting
Aims: Spurious platelet counts can be found in acute leukaemias, as a result of the fragmentation of blood cells. Microscopic examination of a blood smear should be performed to detect the presence of these so called pseudoplatelets. When present, the platelet count should be corrected because of th...
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| Veröffentlicht in: | Journal of clinical pathology 2003-10, Vol.56 (10), p.772-774 |
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| creator | van der Meer, W MacKenzie, M A Dinnissen, J W B de Keijzer, M H |
| description | Aims: Spurious platelet counts can be found in acute leukaemias, as a result of the fragmentation of blood cells. Microscopic examination of a blood smear should be performed to detect the presence of these so called pseudoplatelets. When present, the platelet count should be corrected because of the important clinical consequences that a lower platelet count may have in these patients. Methods: K3EDTA anticoagulated blood was measured on an automated blood cell counter, and a blood smear was made and stained according the May Grünwald–Giemsa method for microscopic observation. A 500 cell/particle differentiation was performed and the automated platelet count was corrected. Results: The incidence of pseudoplatelets in 169 patients with acute leukaemia was studied. Pseudoplatelets were detected in 43 patients (25.4%), and seven patients (4.1%) were re-classified as having a major bleeding risk (platelet count, < 15 × 109/litre). Conclusions: Platelets should be determined morphologically in patients with acute leukaemia and a routine screening method for the detection of pseudoplatelets should be developed. |
| doi_str_mv | 10.1136/jcp.56.10.772 |
| format | Article |
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Microscopic examination of a blood smear should be performed to detect the presence of these so called pseudoplatelets. When present, the platelet count should be corrected because of the important clinical consequences that a lower platelet count may have in these patients. Methods: K3EDTA anticoagulated blood was measured on an automated blood cell counter, and a blood smear was made and stained according the May Grünwald–Giemsa method for microscopic observation. A 500 cell/particle differentiation was performed and the automated platelet count was corrected. Results: The incidence of pseudoplatelets in 169 patients with acute leukaemia was studied. Pseudoplatelets were detected in 43 patients (25.4%), and seven patients (4.1%) were re-classified as having a major bleeding risk (platelet count, < 15 × 109/litre). Conclusions: Platelets should be determined morphologically in patients with acute leukaemia and a routine screening method for the detection of pseudoplatelets should be developed.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.56.10.772</identifier><identifier>PMID: 14514782</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>Acute Disease ; acute leukaemia ; acute lymphoblastic leukaemia ; acute myeloid leukaemia ; Adult ; Aged ; Aged, 80 and over ; ALL ; AML ; Biological and medical sciences ; bleeding risk ; Blood cells ; Blood Cells - pathology ; Blood platelets ; Care and treatment ; Causes of ; CBC ; Child ; Clinical pathology ; complete blood cell count ; Diagnosis ; DIC ; disseminated intravascular coagulation ; Female ; Health aspects ; Hematologic and hematopoietic diseases ; Humans ; Immunohistochemistry ; Leukemia ; Leukemia, Myeloid - blood ; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis ; Male ; Medical sciences ; Medical screening ; Methods ; Microscope and microscopy ; Middle Aged ; Original ; Patients ; Physiological aspects ; Platelet Count ; platelet counts ; Precursor Cell Lymphoblastic Leukemia-Lymphoma - blood ; Prevention ; pseudoplatelets ; Retrospective Studies ; Sensitivity and Specificity ; Subcellular Fractions</subject><ispartof>Journal of clinical pathology, 2003-10, Vol.56 (10), p.772-774</ispartof><rights>Copyright 2003 Journal of Clinical Pathology</rights><rights>2004 INIST-CNRS</rights><rights>COPYRIGHT 2003 BMJ Publishing Group Ltd.</rights><rights>Copyright: 2003 Copyright 2003 Journal of Clinical Pathology</rights><rights>Copyright © Copyright 2003 Journal of Clinical Pathology 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b589t-d8e0f27c965f83a1b4c3634c4d6ba4da9da12411bdaf34dd5dfbb7395c344c73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1770090/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1770090/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15180569$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14514782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Meer, W</creatorcontrib><creatorcontrib>MacKenzie, M A</creatorcontrib><creatorcontrib>Dinnissen, J W B</creatorcontrib><creatorcontrib>de Keijzer, M H</creatorcontrib><title>Pseudoplatelets: a retrospective study of their incidence and interference with platelet counting</title><title>Journal of clinical pathology</title><addtitle>J Clin Pathol</addtitle><description>Aims: Spurious platelet counts can be found in acute leukaemias, as a result of the fragmentation of blood cells. Microscopic examination of a blood smear should be performed to detect the presence of these so called pseudoplatelets. When present, the platelet count should be corrected because of the important clinical consequences that a lower platelet count may have in these patients. Methods: K3EDTA anticoagulated blood was measured on an automated blood cell counter, and a blood smear was made and stained according the May Grünwald–Giemsa method for microscopic observation. A 500 cell/particle differentiation was performed and the automated platelet count was corrected. Results: The incidence of pseudoplatelets in 169 patients with acute leukaemia was studied. Pseudoplatelets were detected in 43 patients (25.4%), and seven patients (4.1%) were re-classified as having a major bleeding risk (platelet count, < 15 × 109/litre). Conclusions: Platelets should be determined morphologically in patients with acute leukaemia and a routine screening method for the detection of pseudoplatelets should be developed.</description><subject>Acute Disease</subject><subject>acute leukaemia</subject><subject>acute lymphoblastic leukaemia</subject><subject>acute myeloid leukaemia</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>ALL</subject><subject>AML</subject><subject>Biological and medical sciences</subject><subject>bleeding risk</subject><subject>Blood cells</subject><subject>Blood Cells - pathology</subject><subject>Blood platelets</subject><subject>Care and treatment</subject><subject>Causes of</subject><subject>CBC</subject><subject>Child</subject><subject>Clinical pathology</subject><subject>complete blood cell count</subject><subject>Diagnosis</subject><subject>DIC</subject><subject>disseminated intravascular coagulation</subject><subject>Female</subject><subject>Health aspects</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Leukemia</subject><subject>Leukemia, Myeloid - blood</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Medical screening</subject><subject>Methods</subject><subject>Microscope and microscopy</subject><subject>Middle Aged</subject><subject>Original</subject><subject>Patients</subject><subject>Physiological aspects</subject><subject>Platelet Count</subject><subject>platelet counts</subject><subject>Precursor Cell Lymphoblastic Leukemia-Lymphoma - blood</subject><subject>Prevention</subject><subject>pseudoplatelets</subject><subject>Retrospective Studies</subject><subject>Sensitivity and Specificity</subject><subject>Subcellular Fractions</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkt9rFDEQxxex2LP66KssiOLLnskm2ez6IJRDz0L9AZbSt5BNJnc595IzyVb735vzzp5KQfIQZuaT78xkpiieYDTFmDSvVmozZc00m5zX94oJpryuKKbN_WKCUI2rjtPmuHgY4wohTDgmD4pjTFnm2npSyM8RRu03g0wwQIqvS1kGSMHHDahkr6GMadQ3pTdlWoINpXXKanAKSul0thIEA-GX47tNy_K3Uqn86JJ1i0fFkZFDhMf7-6S4ePf2Yva-Ov80P5udnlc9a7tU6RaQqbnqGmZaInFPFWkIVVQ3vaRadlrimmLca2kI1Zpp0_ecdEwRShUnJ8Wbnexm7NegFbgU5CA2wa5luBFeWvF3xNmlWPhrgTlHqENZ4MVeIPhvI8Qk1jYqGAbpwI9RcMbrmjCWwWf_gCs_Bpd7y1otxjUi3baeakct5ADCOuNzVrUABzm5d2Bsdp9ijFpcM04zP72Dz0fD2qo7H-wTqDysGMDc9oqR2K6GyKshWLM182pk_umfH3Sg97uQged7QEYlBxNkHnU8cAy3iDXdIbGNCX7cxmX4KhpOOBMfL2fiAyFXV5df5mKe-Zc7vl-v_lPjTzdO36U</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>van der Meer, W</creator><creator>MacKenzie, M A</creator><creator>Dinnissen, J W B</creator><creator>de Keijzer, M H</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ</general><general>BMJ Publishing Group Ltd</general><general>BMJ Publishing Group LTD</general><general>Copyright 2003 Journal of Clinical Pathology</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20031001</creationdate><title>Pseudoplatelets: a retrospective study of their incidence and interference with platelet counting</title><author>van der Meer, W ; MacKenzie, M A ; Dinnissen, J W B ; de Keijzer, M H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b589t-d8e0f27c965f83a1b4c3634c4d6ba4da9da12411bdaf34dd5dfbb7395c344c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Acute Disease</topic><topic>acute leukaemia</topic><topic>acute lymphoblastic leukaemia</topic><topic>acute myeloid leukaemia</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>ALL</topic><topic>AML</topic><topic>Biological and medical sciences</topic><topic>bleeding risk</topic><topic>Blood cells</topic><topic>Blood Cells - pathology</topic><topic>Blood platelets</topic><topic>Care and treatment</topic><topic>Causes of</topic><topic>CBC</topic><topic>Child</topic><topic>Clinical pathology</topic><topic>complete blood cell count</topic><topic>Diagnosis</topic><topic>DIC</topic><topic>disseminated intravascular coagulation</topic><topic>Female</topic><topic>Health aspects</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Leukemia</topic><topic>Leukemia, Myeloid - blood</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Medical screening</topic><topic>Methods</topic><topic>Microscope and microscopy</topic><topic>Middle Aged</topic><topic>Original</topic><topic>Patients</topic><topic>Physiological aspects</topic><topic>Platelet Count</topic><topic>platelet counts</topic><topic>Precursor Cell Lymphoblastic Leukemia-Lymphoma - blood</topic><topic>Prevention</topic><topic>pseudoplatelets</topic><topic>Retrospective Studies</topic><topic>Sensitivity and Specificity</topic><topic>Subcellular Fractions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>van der Meer, W</creatorcontrib><creatorcontrib>MacKenzie, M A</creatorcontrib><creatorcontrib>Dinnissen, J W B</creatorcontrib><creatorcontrib>de Keijzer, M H</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>van der Meer, W</au><au>MacKenzie, M A</au><au>Dinnissen, J W B</au><au>de Keijzer, M H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pseudoplatelets: a retrospective study of their incidence and interference with platelet counting</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>56</volume><issue>10</issue><spage>772</spage><epage>774</epage><pages>772-774</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract>Aims: Spurious platelet counts can be found in acute leukaemias, as a result of the fragmentation of blood cells. Microscopic examination of a blood smear should be performed to detect the presence of these so called pseudoplatelets. When present, the platelet count should be corrected because of the important clinical consequences that a lower platelet count may have in these patients. Methods: K3EDTA anticoagulated blood was measured on an automated blood cell counter, and a blood smear was made and stained according the May Grünwald–Giemsa method for microscopic observation. A 500 cell/particle differentiation was performed and the automated platelet count was corrected. Results: The incidence of pseudoplatelets in 169 patients with acute leukaemia was studied. Pseudoplatelets were detected in 43 patients (25.4%), and seven patients (4.1%) were re-classified as having a major bleeding risk (platelet count, < 15 × 109/litre). Conclusions: Platelets should be determined morphologically in patients with acute leukaemia and a routine screening method for the detection of pseudoplatelets should be developed.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and Association of Clinical Pathologists</pub><pmid>14514782</pmid><doi>10.1136/jcp.56.10.772</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acute Disease acute leukaemia acute lymphoblastic leukaemia acute myeloid leukaemia Adult Aged Aged, 80 and over ALL AML Biological and medical sciences bleeding risk Blood cells Blood Cells - pathology Blood platelets Care and treatment Causes of CBC Child Clinical pathology complete blood cell count Diagnosis DIC disseminated intravascular coagulation Female Health aspects Hematologic and hematopoietic diseases Humans Immunohistochemistry Leukemia Leukemia, Myeloid - blood Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Male Medical sciences Medical screening Methods Microscope and microscopy Middle Aged Original Patients Physiological aspects Platelet Count platelet counts Precursor Cell Lymphoblastic Leukemia-Lymphoma - blood Prevention pseudoplatelets Retrospective Studies Sensitivity and Specificity Subcellular Fractions |
| title | Pseudoplatelets: a retrospective study of their incidence and interference with platelet counting |
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