Alternative splicing generates two isoforms of the β3‐adrenoceptor which are differentially expressed in mouse tissues
The β3‐adrenoceptor (AR) differs from the β1‐AR and β2‐ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse β3‐AR which differ within the coding region. Reverse transcription/polymerase chain reaction with intron‐spanning primers w...
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| Veröffentlicht in: | British journal of pharmacology 1999-07, Vol.127 (6), p.1525-1531 |
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| container_title | British journal of pharmacology |
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| creator | Evans, B A Papaioannou, M Hamilton, S Summers, R J |
| description | The β3‐adrenoceptor (AR) differs from the β1‐AR and β2‐ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse β3‐AR which differ within the coding region.
Reverse transcription/polymerase chain reaction with intron‐spanning primers was used to demonstrate the splice variant of the mouse β3‐adrenoceptor. The novel β3b‐AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known β3a‐AR (RFDGYEGARPFPT).
β3b‐AR mRNA is differentially expressed in mouse tissues, with levels relative to β3a‐AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.
British Journal of Pharmacology (1999) 127, 1525–1531; doi:10.1038/sj.bjp.0702688 |
| doi_str_mv | 10.1038/sj.bjp.0702688 |
| format | Article |
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Reverse transcription/polymerase chain reaction with intron‐spanning primers was used to demonstrate the splice variant of the mouse β3‐adrenoceptor. The novel β3b‐AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known β3a‐AR (RFDGYEGARPFPT).
β3b‐AR mRNA is differentially expressed in mouse tissues, with levels relative to β3a‐AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.
British Journal of Pharmacology (1999) 127, 1525–1531; doi:10.1038/sj.bjp.0702688</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1038/sj.bjp.0702688</identifier><identifier>PMID: 10455305</identifier><identifier>CODEN: BJPCBM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>adipose tissue ; Biological and medical sciences ; brain ; Catecholaminergic system ; Fundamental and applied biological sciences. Psychology ; ileum ; Medical sciences ; Molecular and cellular biology ; Molecular genetics ; mouse ; Neuropharmacology ; Neurotransmitters. Neurotransmission. Receptors ; Pharmacology. Drug treatments ; splice variants ; Transcription. Transcription factor. Splicing. Rna processing ; β3‐adrenoceptor</subject><ispartof>British journal of pharmacology, 1999-07, Vol.127 (6), p.1525-1531</ispartof><rights>1999 Nature Publishing Group</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1999, Nature Publishing Group 1999 Nature Publishing Group</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1760668/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1760668/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,1412,1428,27905,27906,45555,45556,46390,46814,53772,53774</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1929105$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Evans, B A</creatorcontrib><creatorcontrib>Papaioannou, M</creatorcontrib><creatorcontrib>Hamilton, S</creatorcontrib><creatorcontrib>Summers, R J</creatorcontrib><title>Alternative splicing generates two isoforms of the β3‐adrenoceptor which are differentially expressed in mouse tissues</title><title>British journal of pharmacology</title><description>The β3‐adrenoceptor (AR) differs from the β1‐AR and β2‐ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse β3‐AR which differ within the coding region.
Reverse transcription/polymerase chain reaction with intron‐spanning primers was used to demonstrate the splice variant of the mouse β3‐adrenoceptor. The novel β3b‐AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known β3a‐AR (RFDGYEGARPFPT).
β3b‐AR mRNA is differentially expressed in mouse tissues, with levels relative to β3a‐AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.
British Journal of Pharmacology (1999) 127, 1525–1531; doi:10.1038/sj.bjp.0702688</description><subject>adipose tissue</subject><subject>Biological and medical sciences</subject><subject>brain</subject><subject>Catecholaminergic system</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>ileum</subject><subject>Medical sciences</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>mouse</subject><subject>Neuropharmacology</subject><subject>Neurotransmitters. Neurotransmission. Receptors</subject><subject>Pharmacology. Drug treatments</subject><subject>splice variants</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>β3‐adrenoceptor</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNpVkcFu1DAQhi1U1C6lV84-9JplHCexfUFqq0KRKsEBztasM951lI0jO9vt3ngEnoUH4SF4ElJ1RcVpDt-vTyN9jL0TsBQg9fvcLVfduAQFZaP1K7YQlWqKWmpxwhYAoAohtD5jb3LuAGao6lN2JqCqawn1gh2u-onSgFN4IJ7HPrgwrPmaBko4UebTPvKQo49pm3n0fNoQ__1L_vnxE9tEQ3Q0TjHx_Sa4DcdEvA3e00ymgH1_4PQ4JsqZWh4Gvo27THwKOe8ov2WvPfaZLo73nH3_ePvt5q64__Lp883VfdFJKFVBXtSIJRntWiMNQmUMiEYpampwFYiVkyVKbTQ0RishqhLQayWdVr4ttTxnH5694261pdbNryXs7ZjCFtPBRgz2fzKEjV3HBytUA03zJLg8CjA77H3CwYX8TyBMaQTU80w-z_ahp8MLBvsUyubOzqHsMZS9_nonJCj5F1rbjGk</recordid><startdate>199907</startdate><enddate>199907</enddate><creator>Evans, B A</creator><creator>Papaioannou, M</creator><creator>Hamilton, S</creator><creator>Summers, R J</creator><general>Blackwell Publishing Ltd</general><general>Nature Publishing</general><scope>IQODW</scope><scope>5PM</scope></search><sort><creationdate>199907</creationdate><title>Alternative splicing generates two isoforms of the β3‐adrenoceptor which are differentially expressed in mouse tissues</title><author>Evans, B A ; Papaioannou, M ; Hamilton, S ; Summers, R J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j3027-ef15aa2e98cd939a049901677e650c401bc32a38980698711420af873c87fd283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>adipose tissue</topic><topic>Biological and medical sciences</topic><topic>brain</topic><topic>Catecholaminergic system</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>ileum</topic><topic>Medical sciences</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>mouse</topic><topic>Neuropharmacology</topic><topic>Neurotransmitters. Neurotransmission. Receptors</topic><topic>Pharmacology. Drug treatments</topic><topic>splice variants</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>β3‐adrenoceptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Evans, B A</creatorcontrib><creatorcontrib>Papaioannou, M</creatorcontrib><creatorcontrib>Hamilton, S</creatorcontrib><creatorcontrib>Summers, R J</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Evans, B A</au><au>Papaioannou, M</au><au>Hamilton, S</au><au>Summers, R J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Alternative splicing generates two isoforms of the β3‐adrenoceptor which are differentially expressed in mouse tissues</atitle><jtitle>British journal of pharmacology</jtitle><date>1999-07</date><risdate>1999</risdate><volume>127</volume><issue>6</issue><spage>1525</spage><epage>1531</epage><pages>1525-1531</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><coden>BJPCBM</coden><abstract>The β3‐adrenoceptor (AR) differs from the β1‐AR and β2‐ARs in having introns within and downstream of the coding block. This study demonstrates two splice variants of the mouse β3‐AR which differ within the coding region.
Reverse transcription/polymerase chain reaction with intron‐spanning primers was used to demonstrate the splice variant of the mouse β3‐adrenoceptor. The novel β3b‐AR has 17 amino acids encoded by exon 2 (SSLLREPRHLYTCLGYP) which differ from the 13 in the known β3a‐AR (RFDGYEGARPFPT).
β3b‐AR mRNA is differentially expressed in mouse tissues, with levels relative to β3a‐AR mRNA highest in hypothalamus, cortex and white adipose tissue, and lower in ileum smooth muscle and brown adipose tissue.
British Journal of Pharmacology (1999) 127, 1525–1531; doi:10.1038/sj.bjp.0702688</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10455305</pmid><doi>10.1038/sj.bjp.0702688</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | British journal of pharmacology, 1999-07, Vol.127 (6), p.1525-1531 |
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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; PubMed Central; Alma/SFX Local Collection |
| subjects | adipose tissue Biological and medical sciences brain Catecholaminergic system Fundamental and applied biological sciences. Psychology ileum Medical sciences Molecular and cellular biology Molecular genetics mouse Neuropharmacology Neurotransmitters. Neurotransmission. Receptors Pharmacology. Drug treatments splice variants Transcription. Transcription factor. Splicing. Rna processing β3‐adrenoceptor |
| title | Alternative splicing generates two isoforms of the β3‐adrenoceptor which are differentially expressed in mouse tissues |
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