Enteropathogenic Escherichia coli O103 from rabbit elicits actin stress fibers and focal adhesions in HeLa epithelial cells, cytopathic effects that are linked to an analog of the locus of enterocyte effacement
Escherichia coli O103, a major agent of weaned-rabbit diarrhea in Western Europe, was previously shown to produce diarrhea and attaching-and-effacing intestinal lesions in experimentally infected rabbits and to possess a homolog of the eaeA gene of enteropathogenic E. coli (EPEC). In the present stu...
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| description | Escherichia coli O103, a major agent of weaned-rabbit diarrhea in Western Europe, was previously shown to produce diarrhea and attaching-and-effacing intestinal lesions in experimentally infected rabbits and to possess a homolog of the eaeA gene of enteropathogenic E. coli (EPEC). In the present study, we have shown that although negative in the fluorescent-actin staining test on HeLa cells, prototype rabbit E. coli O103 strain B10 was able to induce an original cytopathic effect (CPE) in the same interaction model. This CPE was characterized by a generalized reorganization of the actin cytoskeleton and the formation of focal adhesions on the entire surface of the target cells. These effects amplified with time, leading to cell death about 5 days after the interaction. They were produced by all rabbit E. coli O103 strains tested, by rabbit-infecting E. coli RDEC-1, and also by two human EPEC isolates. We localized genes associated with CPE by using TnphoA insertion mutagenesis in strain B10. In all five independent CPE-negative mutants that we were able to generate, the insertion was located in a region of the genome homologous to the 35-kb locus of enterocyte effacement (LEE locus) of EPEC E2348/69. The mutants concurrently lost the ability to secrete four major supernatant proteins of 25, 37, 39, and 40 kDa, which were shown by immunoprecipitation to share antigenic determinants with secreted proteins of human EPEC E2348/69. The virulence of one of these mutants (strain B10/CA1) was compared with that of the parental strain in the weaned-rabbit diarrhea model. The mutant was totally deprived of virulence, although it colonized the intestine as efficiently as the parental strain did. This study points to a new pathogenic trait of EPEC strains, which is associated with the LEE locus and, possibly, with in vivo virulence |
| doi_str_mv | 10.1128/iai.65.7.2555-2563.1997 |
| format | Article |
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(INRA, Toulouse, France.) ; Comtet, E ; Chalareng, C ; Boury, M ; Tasca, C ; Milon, A</creator><creatorcontrib>Rycke, J.D. (INRA, Toulouse, France.) ; Comtet, E ; Chalareng, C ; Boury, M ; Tasca, C ; Milon, A</creatorcontrib><description>Escherichia coli O103, a major agent of weaned-rabbit diarrhea in Western Europe, was previously shown to produce diarrhea and attaching-and-effacing intestinal lesions in experimentally infected rabbits and to possess a homolog of the eaeA gene of enteropathogenic E. coli (EPEC). In the present study, we have shown that although negative in the fluorescent-actin staining test on HeLa cells, prototype rabbit E. coli O103 strain B10 was able to induce an original cytopathic effect (CPE) in the same interaction model. This CPE was characterized by a generalized reorganization of the actin cytoskeleton and the formation of focal adhesions on the entire surface of the target cells. These effects amplified with time, leading to cell death about 5 days after the interaction. They were produced by all rabbit E. coli O103 strains tested, by rabbit-infecting E. coli RDEC-1, and also by two human EPEC isolates. We localized genes associated with CPE by using TnphoA insertion mutagenesis in strain B10. In all five independent CPE-negative mutants that we were able to generate, the insertion was located in a region of the genome homologous to the 35-kb locus of enterocyte effacement (LEE locus) of EPEC E2348/69. The mutants concurrently lost the ability to secrete four major supernatant proteins of 25, 37, 39, and 40 kDa, which were shown by immunoprecipitation to share antigenic determinants with secreted proteins of human EPEC E2348/69. The virulence of one of these mutants (strain B10/CA1) was compared with that of the parental strain in the weaned-rabbit diarrhea model. The mutant was totally deprived of virulence, although it colonized the intestine as efficiently as the parental strain did. This study points to a new pathogenic trait of EPEC strains, which is associated with the LEE locus and, possibly, with in vivo virulence</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/iai.65.7.2555-2563.1997</identifier><identifier>PMID: 9199419</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ACTINA ; ACTINE ; Actins - pharmacology ; Animals ; Bacteriology ; Biological and medical sciences ; Cell Adhesion ; Cell Death ; CONEJO (ORYCTOLAGUS) ; Cytopathogenic Effect, Viral - genetics ; Epithelial Cells ; ESCHERICHIA COLI ; Escherichia coli - genetics ; Escherichia coli - pathogenicity ; Escherichia coli Infections - pathology ; ESTRES ; Fundamental and applied biological sciences. Psychology ; HeLa Cells ; Humans ; Intestines - pathology ; LAPIN (ORYCTOLAGUS) ; Life Sciences ; Microbiology ; Mutagenesis ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Rabbits ; STRESS ; Virulence - genetics</subject><ispartof>Infection and Immunity, 1997-07, Vol.65 (7), p.2555-2563</ispartof><rights>1997 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c604t-869cd01d175fccd6d0cf1ca4da6cbe87047be49be8e63d91dc18f08b344075aa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC175361/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC175361/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2714024$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9199419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02685741$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Rycke, J.D. (INRA, Toulouse, France.)</creatorcontrib><creatorcontrib>Comtet, E</creatorcontrib><creatorcontrib>Chalareng, C</creatorcontrib><creatorcontrib>Boury, M</creatorcontrib><creatorcontrib>Tasca, C</creatorcontrib><creatorcontrib>Milon, A</creatorcontrib><title>Enteropathogenic Escherichia coli O103 from rabbit elicits actin stress fibers and focal adhesions in HeLa epithelial cells, cytopathic effects that are linked to an analog of the locus of enterocyte effacement</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>Escherichia coli O103, a major agent of weaned-rabbit diarrhea in Western Europe, was previously shown to produce diarrhea and attaching-and-effacing intestinal lesions in experimentally infected rabbits and to possess a homolog of the eaeA gene of enteropathogenic E. coli (EPEC). In the present study, we have shown that although negative in the fluorescent-actin staining test on HeLa cells, prototype rabbit E. coli O103 strain B10 was able to induce an original cytopathic effect (CPE) in the same interaction model. This CPE was characterized by a generalized reorganization of the actin cytoskeleton and the formation of focal adhesions on the entire surface of the target cells. These effects amplified with time, leading to cell death about 5 days after the interaction. They were produced by all rabbit E. coli O103 strains tested, by rabbit-infecting E. coli RDEC-1, and also by two human EPEC isolates. We localized genes associated with CPE by using TnphoA insertion mutagenesis in strain B10. In all five independent CPE-negative mutants that we were able to generate, the insertion was located in a region of the genome homologous to the 35-kb locus of enterocyte effacement (LEE locus) of EPEC E2348/69. The mutants concurrently lost the ability to secrete four major supernatant proteins of 25, 37, 39, and 40 kDa, which were shown by immunoprecipitation to share antigenic determinants with secreted proteins of human EPEC E2348/69. The virulence of one of these mutants (strain B10/CA1) was compared with that of the parental strain in the weaned-rabbit diarrhea model. The mutant was totally deprived of virulence, although it colonized the intestine as efficiently as the parental strain did. 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Psychology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Intestines - pathology</subject><subject>LAPIN (ORYCTOLAGUS)</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Mutagenesis</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Rabbits</subject><subject>STRESS</subject><subject>Virulence - genetics</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUt2K1DAULqKs4-oLCGIEEQQ7Jm2athdeLMvoCAN7oXsdTtOTabTTjElmZV_TJ_J0Z1hcoZAm399J-2XZa8GXQhTNRwduqaplvSyqqsqLSpVL0bb1o2wheNvkVVUUj7MF56LN20rVT7NnMf6grZSyOcvOWiJL0S6yP6spYfB7SIPf4uQMW0UzYHBmcMCMHx27ErxkNvgdC9B1LjEcnXEpMjDJTSymgDEy6zoMdDb1zHoDI4N-wOj8FBmR1rgBhnuXBhITaHAc4wdmbtNdNMWitWjINA2QGARko5t-Ys-SJ096YPRb5i3hBHlziPMG74YnF5z1YHBHJ8-zJxbGiC9O63l2_Xn1_XKdb66-fL282ORGcZnyRrWm56IXdWWN6VXPjRUGZA_KdNjUXNYdypZeUZV9K3ojGsubrpSS1xVAeZ59OvruD90Oe0PRAUa9D24H4VZ7cPohMrlBb_2NpsRSCdK_P-qH_1Tri42ez3ihmqqW4mbmvjtlBf_rgDHpnYvzR4QJ_SFqoXhdNqIgYn0kmuBjDGjvnQXXc3M0NUerStd6bo6em6Pn5pDy1b_XudedqkL42xMOkf6vDTAZF-9pRS0kLyTR3pxu5bbDbxdQQ9w9DCXOyyPHgtewDWRz_Y1GUJym-QuMWuXn</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>Rycke, J.D. (INRA, Toulouse, France.)</creator><creator>Comtet, E</creator><creator>Chalareng, C</creator><creator>Boury, M</creator><creator>Tasca, C</creator><creator>Milon, A</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>1XC</scope><scope>5PM</scope></search><sort><creationdate>19970701</creationdate><title>Enteropathogenic Escherichia coli O103 from rabbit elicits actin stress fibers and focal adhesions in HeLa epithelial cells, cytopathic effects that are linked to an analog of the locus of enterocyte effacement</title><author>Rycke, J.D. 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Psychology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Intestines - pathology</topic><topic>LAPIN (ORYCTOLAGUS)</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Mutagenesis</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Rabbits</topic><topic>STRESS</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rycke, J.D. (INRA, Toulouse, France.)</creatorcontrib><creatorcontrib>Comtet, E</creatorcontrib><creatorcontrib>Chalareng, C</creatorcontrib><creatorcontrib>Boury, M</creatorcontrib><creatorcontrib>Tasca, C</creatorcontrib><creatorcontrib>Milon, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Infection and Immunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rycke, J.D. (INRA, Toulouse, France.)</au><au>Comtet, E</au><au>Chalareng, C</au><au>Boury, M</au><au>Tasca, C</au><au>Milon, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enteropathogenic Escherichia coli O103 from rabbit elicits actin stress fibers and focal adhesions in HeLa epithelial cells, cytopathic effects that are linked to an analog of the locus of enterocyte effacement</atitle><jtitle>Infection and Immunity</jtitle><addtitle>Infect Immun</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>65</volume><issue>7</issue><spage>2555</spage><epage>2563</epage><pages>2555-2563</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>Escherichia coli O103, a major agent of weaned-rabbit diarrhea in Western Europe, was previously shown to produce diarrhea and attaching-and-effacing intestinal lesions in experimentally infected rabbits and to possess a homolog of the eaeA gene of enteropathogenic E. coli (EPEC). In the present study, we have shown that although negative in the fluorescent-actin staining test on HeLa cells, prototype rabbit E. coli O103 strain B10 was able to induce an original cytopathic effect (CPE) in the same interaction model. This CPE was characterized by a generalized reorganization of the actin cytoskeleton and the formation of focal adhesions on the entire surface of the target cells. These effects amplified with time, leading to cell death about 5 days after the interaction. They were produced by all rabbit E. coli O103 strains tested, by rabbit-infecting E. coli RDEC-1, and also by two human EPEC isolates. We localized genes associated with CPE by using TnphoA insertion mutagenesis in strain B10. In all five independent CPE-negative mutants that we were able to generate, the insertion was located in a region of the genome homologous to the 35-kb locus of enterocyte effacement (LEE locus) of EPEC E2348/69. The mutants concurrently lost the ability to secrete four major supernatant proteins of 25, 37, 39, and 40 kDa, which were shown by immunoprecipitation to share antigenic determinants with secreted proteins of human EPEC E2348/69. The virulence of one of these mutants (strain B10/CA1) was compared with that of the parental strain in the weaned-rabbit diarrhea model. The mutant was totally deprived of virulence, although it colonized the intestine as efficiently as the parental strain did. This study points to a new pathogenic trait of EPEC strains, which is associated with the LEE locus and, possibly, with in vivo virulence</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>9199419</pmid><doi>10.1128/iai.65.7.2555-2563.1997</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Infection and Immunity, 1997-07, Vol.65 (7), p.2555-2563 |
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| source | American Society for Microbiology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | ACTINA ACTINE Actins - pharmacology Animals Bacteriology Biological and medical sciences Cell Adhesion Cell Death CONEJO (ORYCTOLAGUS) Cytopathogenic Effect, Viral - genetics Epithelial Cells ESCHERICHIA COLI Escherichia coli - genetics Escherichia coli - pathogenicity Escherichia coli Infections - pathology ESTRES Fundamental and applied biological sciences. Psychology HeLa Cells Humans Intestines - pathology LAPIN (ORYCTOLAGUS) Life Sciences Microbiology Mutagenesis Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Rabbits STRESS Virulence - genetics |
| title | Enteropathogenic Escherichia coli O103 from rabbit elicits actin stress fibers and focal adhesions in HeLa epithelial cells, cytopathic effects that are linked to an analog of the locus of enterocyte effacement |
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