Hemolytic activity of the Pasteurella haemolytica leukotoxin

A Pasteurella haemolytica mutant incapable of producing leukotoxin was created by allelic replacement. Concentrated culture supernatants from wild-type P. haemolytica, but not from the mutant, contained the 102-kDa leukotoxin protein and lysed bovine lymphoma cells and sheep erythrocytes. Wild-type...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Infection and Immunity 1995-08, Vol.63 (8), p.3209-3212
Hauptverfasser:	Murphy, G.L. (Oklahoma State University, Stillwater, OK.), Whitworth, L.C, Clinkenbeard, K.D, Clinkenbeard, P.A
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Toxins - toxicity Bacteriology Biological and medical sciences CONEJO (ORYCTOLAGUS) DNA, Bacterial - genetics ERITROCITOS ERYTHROCYTE Exotoxins - toxicity Fundamental and applied biological sciences. Psychology Genes, Bacterial HEMOLISIS HEMOLYSE Hemolysin Proteins In Vitro Techniques LAPIN (ORYCTOLAGUS) Mannheimia haemolytica - pathogenicity Microbiology Mutagenesis MUTANT MUTANTES OVIN OVINOS PASTEURELLA HAEMOLYTICA Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Rabbits Sheep TOXINAS BACTERIANAS TOXINE BACTERIENNE
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A Pasteurella haemolytica mutant incapable of producing leukotoxin was created by allelic replacement. Concentrated culture supernatants from wild-type P. haemolytica, but not from the mutant, contained the 102-kDa leukotoxin protein and lysed bovine lymphoma cells and sheep erythrocytes. Wild-type P. haemolytica demonstrated the typical beta-hemolytic phenotype on sheep and rabbit blood agar, whereas the mutant did not
ISSN:	0019-9567 1098-5522
DOI:	10.1128/iai.63.8.3209-3212.1995