Apoptosis and apoptosis related gene expression in normal conjunctiva and pterygium
BACKGROUND Pterygium is a relatively common eye disease in the tropics whose aetiology and pathogenesis remain uncertain. As such, interest has focused on understanding the underlying mechanism of pterygia development. METHODS 15 specimens of pterygia from 15 eyes were examined, together with normal...
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| Veröffentlicht in: | British journal of ophthalmology 2000-02, Vol.84 (2), p.212-216 |
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| description | BACKGROUND Pterygium is a relatively common eye disease in the tropics whose aetiology and pathogenesis remain uncertain. As such, interest has focused on understanding the underlying mechanism of pterygia development. METHODS 15 specimens of pterygia from 15 eyes were examined, together with normal conjunctival tissue from the same eyes for the pattern of gene expression of genes associated with the induction or repression of apoptosis (p53, bcl-2, and bax). In addition, the samples directly for apoptotic cells were examined by the terminal deoxynucleotide transferase (TdT) mediated nick end labelling (TUNEL) methodology. RESULTS In pterygia specimens apoptotic cells were found mainly confined to the basal layer of cells of the epithelial layer, situated immediately adjacent to the fibrovascular support layer. These cells were shown to express significant levels of p53 and bax, as well as the apoptosis inhibiting protein bcl-2. In contrast, normal conjunctival specimens displayed no bcl-2 expression and apoptotic cells were seen throughout the entire width of the epithelial layer, coupled with high levels of bax expression. CONCLUSION These results support a model whereby pterygia development is a result of disruption of the normal process of apoptosis occurring in the conjunctiva. |
| doi_str_mv | 10.1136/bjo.84.2.212 |
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As such, interest has focused on understanding the underlying mechanism of pterygia development. METHODS 15 specimens of pterygia from 15 eyes were examined, together with normal conjunctival tissue from the same eyes for the pattern of gene expression of genes associated with the induction or repression of apoptosis (p53, bcl-2, and bax). In addition, the samples directly for apoptotic cells were examined by the terminal deoxynucleotide transferase (TdT) mediated nick end labelling (TUNEL) methodology. RESULTS In pterygia specimens apoptotic cells were found mainly confined to the basal layer of cells of the epithelial layer, situated immediately adjacent to the fibrovascular support layer. These cells were shown to express significant levels of p53 and bax, as well as the apoptosis inhibiting protein bcl-2. In contrast, normal conjunctival specimens displayed no bcl-2 expression and apoptotic cells were seen throughout the entire width of the epithelial layer, coupled with high levels of bax expression. CONCLUSION These results support a model whereby pterygia development is a result of disruption of the normal process of apoptosis occurring in the conjunctiva.</description><identifier>ISSN: 0007-1161</identifier><identifier>EISSN: 1468-2079</identifier><identifier>DOI: 10.1136/bjo.84.2.212</identifier><identifier>PMID: 10655200</identifier><identifier>CODEN: BJOPAL</identifier><language>eng</language><publisher>BMA House, Tavistock Square, London, WC1H 9JR: BMJ Publishing Group Ltd</publisher><subject>Apoptosis ; Apoptosis - genetics ; Apoptosis - physiology ; bcl-2-Associated X Protein ; Biological and medical sciences ; conjunctiva ; Conjunctiva - physiology ; Diseases of eyelid, conjunctiva and lacrimal tracts ; Gene Expression ; Humans ; Immunohistochemistry ; In Situ Nick-End Labeling ; Medical sciences ; Methods ; Ophthalmology ; Original articles - Laboratory science ; Polyclonal antibodies ; Proteins ; Proto-Oncogene Proteins - analysis ; Proto-Oncogene Proteins c-bcl-2 - analysis ; pterygium ; Pterygium - genetics ; Pterygium - physiopathology ; Tumor Suppressor Protein p53 - analysis</subject><ispartof>British journal of ophthalmology, 2000-02, Vol.84 (2), p.212-216</ispartof><rights>British Journal of Ophthalmology</rights><rights>2000 INIST-CNRS</rights><rights>Copyright: 2000 British Journal of Ophthalmology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b573t-32e7d23e6aeab8f84ff0a1c99b32789bfdd2ee8244016f6f44a467e8a4a2f82b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1723383/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1723383/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1267836$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10655200$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tan, Donald T H</creatorcontrib><creatorcontrib>Tang, Wen Ying</creatorcontrib><creatorcontrib>Liu, Yan Ping</creatorcontrib><creatorcontrib>Goh, Hak-Su</creatorcontrib><creatorcontrib>Smith, Duncan R</creatorcontrib><title>Apoptosis and apoptosis related gene expression in normal conjunctiva and pterygium</title><title>British journal of ophthalmology</title><addtitle>Br J Ophthalmol</addtitle><description>BACKGROUND Pterygium is a relatively common eye disease in the tropics whose aetiology and pathogenesis remain uncertain. As such, interest has focused on understanding the underlying mechanism of pterygia development. METHODS 15 specimens of pterygia from 15 eyes were examined, together with normal conjunctival tissue from the same eyes for the pattern of gene expression of genes associated with the induction or repression of apoptosis (p53, bcl-2, and bax). In addition, the samples directly for apoptotic cells were examined by the terminal deoxynucleotide transferase (TdT) mediated nick end labelling (TUNEL) methodology. RESULTS In pterygia specimens apoptotic cells were found mainly confined to the basal layer of cells of the epithelial layer, situated immediately adjacent to the fibrovascular support layer. These cells were shown to express significant levels of p53 and bax, as well as the apoptosis inhibiting protein bcl-2. In contrast, normal conjunctival specimens displayed no bcl-2 expression and apoptotic cells were seen throughout the entire width of the epithelial layer, coupled with high levels of bax expression. CONCLUSION These results support a model whereby pterygia development is a result of disruption of the normal process of apoptosis occurring in the conjunctiva.</description><subject>Apoptosis</subject><subject>Apoptosis - genetics</subject><subject>Apoptosis - physiology</subject><subject>bcl-2-Associated X Protein</subject><subject>Biological and medical sciences</subject><subject>conjunctiva</subject><subject>Conjunctiva - physiology</subject><subject>Diseases of eyelid, conjunctiva and lacrimal tracts</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Nick-End Labeling</subject><subject>Medical sciences</subject><subject>Methods</subject><subject>Ophthalmology</subject><subject>Original articles - Laboratory science</subject><subject>Polyclonal antibodies</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins - analysis</subject><subject>Proto-Oncogene Proteins c-bcl-2 - analysis</subject><subject>pterygium</subject><subject>Pterygium - genetics</subject><subject>Pterygium - physiopathology</subject><subject>Tumor Suppressor Protein p53 - analysis</subject><issn>0007-1161</issn><issn>1468-2079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNp90Utv1DAUBWALgei0sGONIoFgQwa_YjsbpGpaKKICidfWcpLrwUNiBzup2n-PIaOhsGBlXfnz1bEOQo8IXhPCxMtmF9aKr-maEnoHrQgXqqRY1nfRCmMsS0IEOULHKe3ySAWR99ERwaKqKMYr9Ol0DOMUkkuF8V1hDlOE3kzQFVvwUMD1GCElF3zhfOFDHExftMHvZt9O7sr8fjtOEG-2bh4eoHvW9Ake7s8T9OX1-efNRXn54c3bzell2VSSTSWjIDvKQBgwjbKKW4sNaeu6YVSqurFdRwEU5RwTYYXl3HAhQRluqFW0YSfo1bJ3nJsBuhb8FE2vx-gGE290ME7_fePdN70NV5pIyphiecGz_YIYfsyQJj241ELfGw9hTlpiJWuheIZP_oG7MEefP5d35ayCV3WV1YtFtTGkFMEeohCsf3Wlc1dacU117irzx7fj38JLORk83QOTWtPbaHzr0h9HhVRMZFYuzKUJrg_XJn7XQjJZ6fdfN1qKj_js7ALrd9k_X3wz7P6f8Cel-7nv</recordid><startdate>20000201</startdate><enddate>20000201</enddate><creator>Tan, Donald T H</creator><creator>Tang, Wen Ying</creator><creator>Liu, Yan Ping</creator><creator>Goh, Hak-Su</creator><creator>Smith, Duncan R</creator><general>BMJ Publishing Group Ltd</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20000201</creationdate><title>Apoptosis and apoptosis related gene expression in normal conjunctiva and pterygium</title><author>Tan, Donald T H ; Tang, Wen Ying ; Liu, Yan Ping ; Goh, Hak-Su ; Smith, Duncan R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b573t-32e7d23e6aeab8f84ff0a1c99b32789bfdd2ee8244016f6f44a467e8a4a2f82b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Apoptosis</topic><topic>Apoptosis - genetics</topic><topic>Apoptosis - physiology</topic><topic>bcl-2-Associated X Protein</topic><topic>Biological and medical sciences</topic><topic>conjunctiva</topic><topic>Conjunctiva - physiology</topic><topic>Diseases of eyelid, conjunctiva and lacrimal tracts</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Nick-End Labeling</topic><topic>Medical sciences</topic><topic>Methods</topic><topic>Ophthalmology</topic><topic>Original articles - Laboratory science</topic><topic>Polyclonal antibodies</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins - analysis</topic><topic>Proto-Oncogene Proteins c-bcl-2 - analysis</topic><topic>pterygium</topic><topic>Pterygium - genetics</topic><topic>Pterygium - physiopathology</topic><topic>Tumor Suppressor Protein p53 - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tan, Donald T H</creatorcontrib><creatorcontrib>Tang, Wen Ying</creatorcontrib><creatorcontrib>Liu, Yan Ping</creatorcontrib><creatorcontrib>Goh, Hak-Su</creatorcontrib><creatorcontrib>Smith, Duncan R</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tan, Donald T H</au><au>Tang, Wen Ying</au><au>Liu, Yan Ping</au><au>Goh, Hak-Su</au><au>Smith, Duncan R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Apoptosis and apoptosis related gene expression in normal conjunctiva and pterygium</atitle><jtitle>British journal of ophthalmology</jtitle><addtitle>Br J Ophthalmol</addtitle><date>2000-02-01</date><risdate>2000</risdate><volume>84</volume><issue>2</issue><spage>212</spage><epage>216</epage><pages>212-216</pages><issn>0007-1161</issn><eissn>1468-2079</eissn><coden>BJOPAL</coden><abstract>BACKGROUND Pterygium is a relatively common eye disease in the tropics whose aetiology and pathogenesis remain uncertain. As such, interest has focused on understanding the underlying mechanism of pterygia development. METHODS 15 specimens of pterygia from 15 eyes were examined, together with normal conjunctival tissue from the same eyes for the pattern of gene expression of genes associated with the induction or repression of apoptosis (p53, bcl-2, and bax). In addition, the samples directly for apoptotic cells were examined by the terminal deoxynucleotide transferase (TdT) mediated nick end labelling (TUNEL) methodology. RESULTS In pterygia specimens apoptotic cells were found mainly confined to the basal layer of cells of the epithelial layer, situated immediately adjacent to the fibrovascular support layer. These cells were shown to express significant levels of p53 and bax, as well as the apoptosis inhibiting protein bcl-2. In contrast, normal conjunctival specimens displayed no bcl-2 expression and apoptotic cells were seen throughout the entire width of the epithelial layer, coupled with high levels of bax expression. CONCLUSION These results support a model whereby pterygia development is a result of disruption of the normal process of apoptosis occurring in the conjunctiva.</abstract><cop>BMA House, Tavistock Square, London, WC1H 9JR</cop><pub>BMJ Publishing Group Ltd</pub><pmid>10655200</pmid><doi>10.1136/bjo.84.2.212</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis Apoptosis - genetics Apoptosis - physiology bcl-2-Associated X Protein Biological and medical sciences conjunctiva Conjunctiva - physiology Diseases of eyelid, conjunctiva and lacrimal tracts Gene Expression Humans Immunohistochemistry In Situ Nick-End Labeling Medical sciences Methods Ophthalmology Original articles - Laboratory science Polyclonal antibodies Proteins Proto-Oncogene Proteins - analysis Proto-Oncogene Proteins c-bcl-2 - analysis pterygium Pterygium - genetics Pterygium - physiopathology Tumor Suppressor Protein p53 - analysis |
| title | Apoptosis and apoptosis related gene expression in normal conjunctiva and pterygium |
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