Diagnosis of primary human herpesvirus 6 and 7 infections in febrile infants by polymerase chain reaction
Primary human herpesvirus 6 (HHV-6) and 7 (HHV-7) infections were identified in febrile children by qualitative and quantitative polymerase chain reaction (PCR) assays. Diagnosis was based on the differential detection of viral DNA in peripheral blood mononuclear cells (PBMC), but not in saliva. Six...
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| Veröffentlicht in: | Archives of disease in childhood 1997-07, Vol.77 (1), p.42-45 |
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| creator | Clark, Duncan A Kidd, I Michael Collingham, Kathryn E Tarlow, Michael Ayeni, Titi Riordan, Andrew Griffiths, Paul D Emery, Vincent C Pillay, Deenan |
| description | Primary human herpesvirus 6 (HHV-6) and 7 (HHV-7) infections were identified in febrile children by qualitative and quantitative polymerase chain reaction (PCR) assays. Diagnosis was based on the differential detection of viral DNA in peripheral blood mononuclear cells (PBMC), but not in saliva. Six of 41 febrile infants, but none of seven non-febrile controls, were identified with primary infections (three HHV-6, three HHV-7). These children had significantly higher viral loads in PBMC (HHV-6, median 24 213 genomes/106PBMC; HHV-7, median 6 040 000 genomes/106 PBMC) than DNA-aemic, saliva PCR positive children (HHV-6, median 1606 genomes/106 PBMC, p |
| doi_str_mv | 10.1136/adc.77.1.42 |
| format | Article |
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Diagnosis was based on the differential detection of viral DNA in peripheral blood mononuclear cells (PBMC), but not in saliva. Six of 41 febrile infants, but none of seven non-febrile controls, were identified with primary infections (three HHV-6, three HHV-7). These children had significantly higher viral loads in PBMC (HHV-6, median 24 213 genomes/106PBMC; HHV-7, median 6 040 000 genomes/106 PBMC) than DNA-aemic, saliva PCR positive children (HHV-6, median 1606 genomes/106 PBMC, p < 0.01; HHV-7, median 7089 genomes/106 PBMC, p < 0.05). Viral DNA was detected in serum by PCR in only 50% of primary infections. All three children with primary HHV-7 infection had febrile convulsions. Thus PCR, including quantitative assays, may identify primary HHV-6 and HHV-7 infections when an appropriate combination of clinical specimens is used.</description><identifier>ISSN: 0003-9888</identifier><identifier>EISSN: 1468-2044</identifier><identifier>DOI: 10.1136/adc.77.1.42</identifier><identifier>PMID: 9279150</identifier><identifier>CODEN: ADCHAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health</publisher><subject>Age ; AIDS/HIV ; Antigens ; Biological and medical sciences ; Deoxyribonucleic acid ; DNA ; DNA, Viral - analysis ; febrile convulsions ; Genomes ; Herpesviridae Infections - diagnosis ; Herpesvirus 6, Human - genetics ; Herpesvirus 7, Human - genetics ; HHV-6 ; HHV-7 ; Human viral diseases ; Humans ; Illnesses ; Immunoglobulins ; Infant ; Infants ; Infections ; Infectious diseases ; Laboratories ; Leukocytes, Mononuclear - virology ; Medical sciences ; Original ; Plasma ; Polymerase Chain Reaction ; Seizures, Febrile - virology ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids ; Viral infections ; Viral Load ; Viruses ; Young Children</subject><ispartof>Archives of disease in childhood, 1997-07, Vol.77 (1), p.42-45</ispartof><rights>Royal College of Paediatrics and Child Health</rights><rights>1997 INIST-CNRS</rights><rights>Copyright: 1997 Royal College of Paediatrics and Child Health</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b502t-e8e27b9187da80fa909a72b9e3387b9b2e460a9bb446ed4f4d0bf01d5baca86f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1717251/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1717251/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2779768$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9279150$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clark, Duncan A</creatorcontrib><creatorcontrib>Kidd, I Michael</creatorcontrib><creatorcontrib>Collingham, Kathryn E</creatorcontrib><creatorcontrib>Tarlow, Michael</creatorcontrib><creatorcontrib>Ayeni, Titi</creatorcontrib><creatorcontrib>Riordan, Andrew</creatorcontrib><creatorcontrib>Griffiths, Paul D</creatorcontrib><creatorcontrib>Emery, Vincent C</creatorcontrib><creatorcontrib>Pillay, Deenan</creatorcontrib><title>Diagnosis of primary human herpesvirus 6 and 7 infections in febrile infants by polymerase chain reaction</title><title>Archives of disease in childhood</title><addtitle>Arch Dis Child</addtitle><description>Primary human herpesvirus 6 (HHV-6) and 7 (HHV-7) infections were identified in febrile children by qualitative and quantitative polymerase chain reaction (PCR) assays. Diagnosis was based on the differential detection of viral DNA in peripheral blood mononuclear cells (PBMC), but not in saliva. Six of 41 febrile infants, but none of seven non-febrile controls, were identified with primary infections (three HHV-6, three HHV-7). These children had significantly higher viral loads in PBMC (HHV-6, median 24 213 genomes/106PBMC; HHV-7, median 6 040 000 genomes/106 PBMC) than DNA-aemic, saliva PCR positive children (HHV-6, median 1606 genomes/106 PBMC, p < 0.01; HHV-7, median 7089 genomes/106 PBMC, p < 0.05). Viral DNA was detected in serum by PCR in only 50% of primary infections. All three children with primary HHV-7 infection had febrile convulsions. Thus PCR, including quantitative assays, may identify primary HHV-6 and HHV-7 infections when an appropriate combination of clinical specimens is used.</description><subject>Age</subject><subject>AIDS/HIV</subject><subject>Antigens</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Viral - analysis</subject><subject>febrile convulsions</subject><subject>Genomes</subject><subject>Herpesviridae Infections - diagnosis</subject><subject>Herpesvirus 6, Human - genetics</subject><subject>Herpesvirus 7, Human - genetics</subject><subject>HHV-6</subject><subject>HHV-7</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Illnesses</subject><subject>Immunoglobulins</subject><subject>Infant</subject><subject>Infants</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Laboratories</subject><subject>Leukocytes, Mononuclear - virology</subject><subject>Medical sciences</subject><subject>Original</subject><subject>Plasma</subject><subject>Polymerase Chain Reaction</subject><subject>Seizures, Febrile - virology</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><subject>Viral infections</subject><subject>Viral Load</subject><subject>Viruses</subject><subject>Young Children</subject><issn>0003-9888</issn><issn>1468-2044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kc2P0zAQxSMEWsrCiTOSJRAXlGI7TmxfkFBhWdAKLrAHLtY4GW9dEqfYyYr-97i0Kh8HTrbm_fw8T68oHjO6ZKxqXkLXLqVcsqXgd4oFE40qORXibrGglFalVkrdLx6ktKGUcaWqs-JMc6lZTReFf-PhJozJJzI6so1-gLgj63mAQNYYt5hufZwTaQiEjkjig8N28mNI-Uoc2uh73E8hTInYHdmO_W7ACAlJu4bMRIRfDx4W9xz0CR8dz_Piy8Xbz6vL8urTu_er11elrSmfSlTIpdVMyQ4UdaCpBsmtxqpSeW45ioaCtlaIBjvhREeto6yrLbSgGledF68OvtvZDti1GKYIvTlGMyN487cS_NrcjLeGSSZ5zbLB86NBHL_PmCYz-NRi30PAcU5Gai4axlQGn_4DbsY5hhwue0lFFWfVnnpxoNo4phTRnVZh1Oz7M7k_I6VhRvBMP_lz-xN7LCzrz446pBZ6FyG0Pp0wLqWWzf7T8oD5NOGPkwzxm2lkJWvz8XplWP2h-np5fWHU78x22Px3v5-FEMBU</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>Clark, Duncan A</creator><creator>Kidd, I Michael</creator><creator>Collingham, Kathryn E</creator><creator>Tarlow, Michael</creator><creator>Ayeni, Titi</creator><creator>Riordan, Andrew</creator><creator>Griffiths, Paul D</creator><creator>Emery, Vincent C</creator><creator>Pillay, Deenan</creator><general>BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health</general><general>BMJ</general><general>BMJ Publishing Group LTD</general><general>BMJ Group</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>0-V</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88B</scope><scope>88E</scope><scope>88I</scope><scope>8A4</scope><scope>8AF</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ALSLI</scope><scope>AN0</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>CJNVE</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>LK8</scope><scope>M0P</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEDU</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970701</creationdate><title>Diagnosis of primary human herpesvirus 6 and 7 infections in febrile infants by polymerase chain reaction</title><author>Clark, Duncan A ; Kidd, I Michael ; Collingham, Kathryn E ; Tarlow, Michael ; Ayeni, Titi ; Riordan, Andrew ; Griffiths, Paul D ; Emery, Vincent C ; Pillay, Deenan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b502t-e8e27b9187da80fa909a72b9e3387b9b2e460a9bb446ed4f4d0bf01d5baca86f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Age</topic><topic>AIDS/HIV</topic><topic>Antigens</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Viral - analysis</topic><topic>febrile convulsions</topic><topic>Genomes</topic><topic>Herpesviridae Infections - diagnosis</topic><topic>Herpesvirus 6, Human - genetics</topic><topic>Herpesvirus 7, Human - genetics</topic><topic>HHV-6</topic><topic>HHV-7</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Illnesses</topic><topic>Immunoglobulins</topic><topic>Infant</topic><topic>Infants</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Laboratories</topic><topic>Leukocytes, Mononuclear - virology</topic><topic>Medical sciences</topic><topic>Original</topic><topic>Plasma</topic><topic>Polymerase Chain Reaction</topic><topic>Seizures, Febrile - virology</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><topic>Viral infections</topic><topic>Viral Load</topic><topic>Viruses</topic><topic>Young Children</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clark, Duncan A</creatorcontrib><creatorcontrib>Kidd, I Michael</creatorcontrib><creatorcontrib>Collingham, Kathryn E</creatorcontrib><creatorcontrib>Tarlow, Michael</creatorcontrib><creatorcontrib>Ayeni, Titi</creatorcontrib><creatorcontrib>Riordan, Andrew</creatorcontrib><creatorcontrib>Griffiths, Paul D</creatorcontrib><creatorcontrib>Emery, Vincent C</creatorcontrib><creatorcontrib>Pillay, Deenan</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Social Sciences Premium Collection</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Education Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Education Periodicals</collection><collection>STEM Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Social Science Premium Collection</collection><collection>British Nursing Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Education Collection</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Education Database</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Education</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Archives of disease in childhood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clark, Duncan A</au><au>Kidd, I Michael</au><au>Collingham, Kathryn E</au><au>Tarlow, Michael</au><au>Ayeni, Titi</au><au>Riordan, Andrew</au><au>Griffiths, Paul D</au><au>Emery, Vincent C</au><au>Pillay, Deenan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnosis of primary human herpesvirus 6 and 7 infections in febrile infants by polymerase chain reaction</atitle><jtitle>Archives of disease in childhood</jtitle><addtitle>Arch Dis Child</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>77</volume><issue>1</issue><spage>42</spage><epage>45</epage><pages>42-45</pages><issn>0003-9888</issn><eissn>1468-2044</eissn><coden>ADCHAK</coden><abstract>Primary human herpesvirus 6 (HHV-6) and 7 (HHV-7) infections were identified in febrile children by qualitative and quantitative polymerase chain reaction (PCR) assays. Diagnosis was based on the differential detection of viral DNA in peripheral blood mononuclear cells (PBMC), but not in saliva. Six of 41 febrile infants, but none of seven non-febrile controls, were identified with primary infections (three HHV-6, three HHV-7). These children had significantly higher viral loads in PBMC (HHV-6, median 24 213 genomes/106PBMC; HHV-7, median 6 040 000 genomes/106 PBMC) than DNA-aemic, saliva PCR positive children (HHV-6, median 1606 genomes/106 PBMC, p < 0.01; HHV-7, median 7089 genomes/106 PBMC, p < 0.05). Viral DNA was detected in serum by PCR in only 50% of primary infections. All three children with primary HHV-7 infection had febrile convulsions. Thus PCR, including quantitative assays, may identify primary HHV-6 and HHV-7 infections when an appropriate combination of clinical specimens is used.</abstract><cop>London</cop><pub>BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health</pub><pmid>9279150</pmid><doi>10.1136/adc.77.1.42</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Age AIDS/HIV Antigens Biological and medical sciences Deoxyribonucleic acid DNA DNA, Viral - analysis febrile convulsions Genomes Herpesviridae Infections - diagnosis Herpesvirus 6, Human - genetics Herpesvirus 7, Human - genetics HHV-6 HHV-7 Human viral diseases Humans Illnesses Immunoglobulins Infant Infants Infections Infectious diseases Laboratories Leukocytes, Mononuclear - virology Medical sciences Original Plasma Polymerase Chain Reaction Seizures, Febrile - virology Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Viral infections Viral Load Viruses Young Children |
| title | Diagnosis of primary human herpesvirus 6 and 7 infections in febrile infants by polymerase chain reaction |
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