Regionalized GC content of template DNA as a predictor of PCR success

A set of 1438 human exons was subjected to nested PCR. The initial success rate using a standard PCR protocol required for ligation‐independent cloning was 83.4%. Logistic regression analysis was conducted on 27 primer‐ and template‐related characteristics, of which most could be ignored apart from...

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Veröffentlicht in:	Nucleic acids research 2003-08, Vol.31 (16), p.e99-e99
Hauptverfasser:	Benita, Yair, Oosting, Ronald S., Lok, Martin C., Wise, Michael J., Humphery‐Smith, Ian
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of Variance Base Composition DNA - genetics DNA Primers - genetics GC Rich Sequence Humans Logistic Models NAR Methods Online Polymerase Chain Reaction - methods Sensitivity and Specificity Temperature Templates, Genetic
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