Anti-kinetochore antibodies: use as probes for inactive centromeres

Application of a modified immunofluorescence technique using an anti-kinetochore serum enables cytogeneticists to obtain quality metaphase spreads and to localize kinetochores. In a patient with a 45, XX, -9, -11, tdic (9p;11p) constitution, we found that the dicentric marker chromosome has an inten...

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Veröffentlicht in:	American journal of human genetics 1985-03, Vol.37 (2), p.425-430
Hauptverfasser:	MERRY, D. E, PATHAK, S, HSU, T. C, BRINKLEY, B. R
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies Biological and medical sciences Cell Line Centromere - immunology Centromere - ultrastructure Chromosomes - ultrastructure Cytogenetics Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Human Humans Immune Sera Karyotyping Metaphase
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creator	MERRY, D. E PATHAK, S HSU, T. C BRINKLEY, B. R
description	Application of a modified immunofluorescence technique using an anti-kinetochore serum enables cytogeneticists to obtain quality metaphase spreads and to localize kinetochores. In a patient with a 45, XX, -9, -11, tdic (9p;11p) constitution, we found that the dicentric marker chromosome has an intensely fluorescent kinetochore (no. 11), the functional centromere, and a less intensely fluorescent kinetochore (no. 9), the inactive centromere. The data suggest that in the process of tandem fusion (telomere-telomere between 11p and 9p), the centromere of chromosome 9 was not deleted, but, rather, inactivated.
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The data suggest that in the process of tandem fusion (telomere-telomere between 11p and 9p), the centromere of chromosome 9 was not deleted, but, rather, inactivated.</description><identifier>ISSN: 0002-9297</identifier><identifier>EISSN: 1537-6605</identifier><identifier>PMID: 3885726</identifier><identifier>CODEN: AJHGAG</identifier><language>eng</language><publisher>Chicago, IL: University of Chicago Press</publisher><subject>Antibodies ; Biological and medical sciences ; Cell Line ; Centromere - immunology ; Centromere - ultrastructure ; Chromosomes - ultrastructure ; Cytogenetics ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Genetics of eukaryotes. 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E</creatorcontrib><creatorcontrib>PATHAK, S</creatorcontrib><creatorcontrib>HSU, T. C</creatorcontrib><creatorcontrib>BRINKLEY, B. R</creatorcontrib><title>Anti-kinetochore antibodies: use as probes for inactive centromeres</title><title>American journal of human genetics</title><addtitle>Am J Hum Genet</addtitle><description>Application of a modified immunofluorescence technique using an anti-kinetochore serum enables cytogeneticists to obtain quality metaphase spreads and to localize kinetochores. In a patient with a 45, XX, -9, -11, tdic (9p;11p) constitution, we found that the dicentric marker chromosome has an intensely fluorescent kinetochore (no. 11), the functional centromere, and a less intensely fluorescent kinetochore (no. 9), the inactive centromere. The data suggest that in the process of tandem fusion (telomere-telomere between 11p and 9p), the centromere of chromosome 9 was not deleted, but, rather, inactivated.</description><subject>Antibodies</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Centromere - immunology</subject><subject>Centromere - ultrastructure</subject><subject>Chromosomes - ultrastructure</subject><subject>Cytogenetics</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics of eukaryotes. 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R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p321t-669302439128181fd03c41cb596745c4f31b9922dcbb6c0e03a7d696a1f6e9de3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Antibodies</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Centromere - immunology</topic><topic>Centromere - ultrastructure</topic><topic>Chromosomes - ultrastructure</topic><topic>Cytogenetics</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Human</topic><topic>Humans</topic><topic>Immune Sera</topic><topic>Karyotyping</topic><topic>Metaphase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MERRY, D. E</creatorcontrib><creatorcontrib>PATHAK, S</creatorcontrib><creatorcontrib>HSU, T. C</creatorcontrib><creatorcontrib>BRINKLEY, B. R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>American journal of human genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MERRY, D. E</au><au>PATHAK, S</au><au>HSU, T. C</au><au>BRINKLEY, B. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-kinetochore antibodies: use as probes for inactive centromeres</atitle><jtitle>American journal of human genetics</jtitle><addtitle>Am J Hum Genet</addtitle><date>1985-03-01</date><risdate>1985</risdate><volume>37</volume><issue>2</issue><spage>425</spage><epage>430</epage><pages>425-430</pages><issn>0002-9297</issn><eissn>1537-6605</eissn><coden>AJHGAG</coden><abstract>Application of a modified immunofluorescence technique using an anti-kinetochore serum enables cytogeneticists to obtain quality metaphase spreads and to localize kinetochores. In a patient with a 45, XX, -9, -11, tdic (9p;11p) constitution, we found that the dicentric marker chromosome has an intensely fluorescent kinetochore (no. 11), the functional centromere, and a less intensely fluorescent kinetochore (no. 9), the inactive centromere. The data suggest that in the process of tandem fusion (telomere-telomere between 11p and 9p), the centromere of chromosome 9 was not deleted, but, rather, inactivated.</abstract><cop>Chicago, IL</cop><pub>University of Chicago Press</pub><pmid>3885726</pmid><tpages>6</tpages></addata></record>
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subjects	Antibodies Biological and medical sciences Cell Line Centromere - immunology Centromere - ultrastructure Chromosomes - ultrastructure Cytogenetics Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Human Humans Immune Sera Karyotyping Metaphase
title	Anti-kinetochore antibodies: use as probes for inactive centromeres
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