Induction of sister chromatid exchanges at common fragile sites
Experiments were performed to gain further insight into chromosome structure and behavior at common fragile sites by testing the hypothesis that gaps at these sites predispose to intrachromosomal recombination as measured by sister chromatid exchanges (SCEs). Human lymphocytes were concurrently trea...
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| Veröffentlicht in: | American journal of human genetics 1987-11, Vol.41 (5), p.882-890 |
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| container_title | American journal of human genetics |
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| creator | GLOVER, T. W STEIN, C. K |
| description | Experiments were performed to gain further insight into chromosome structure and behavior at common fragile sites by testing the hypothesis that gaps at these sites predispose to intrachromosomal recombination as measured by sister chromatid exchanges (SCEs). Human lymphocytes were concurrently treated with aphidicolin, for determination of fragile site expression, and with 5-bromodeoxy-uridine, for SCE analysis. Aphidicolin induced chromosome gaps nonrandomly, with the great majority of gaps occurring at common fragile sites. On average, 66% of gaps were accompanied by an SCE at the site of the lesion. Analysis of two specific common fragile sites at 3p14 and 16q23 showed the same pattern; that is, on average 70% of gaps at these sites were accompanied by an SCE. These results show that common fragile sites are hot spots not only for chromosomal lesions such as gaps but also for SCE formation. |
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W ; STEIN, C. K</creator><creatorcontrib>GLOVER, T. W ; STEIN, C. K</creatorcontrib><description>Experiments were performed to gain further insight into chromosome structure and behavior at common fragile sites by testing the hypothesis that gaps at these sites predispose to intrachromosomal recombination as measured by sister chromatid exchanges (SCEs). Human lymphocytes were concurrently treated with aphidicolin, for determination of fragile site expression, and with 5-bromodeoxy-uridine, for SCE analysis. Aphidicolin induced chromosome gaps nonrandomly, with the great majority of gaps occurring at common fragile sites. On average, 66% of gaps were accompanied by an SCE at the site of the lesion. Analysis of two specific common fragile sites at 3p14 and 16q23 showed the same pattern; that is, on average 70% of gaps at these sites were accompanied by an SCE. These results show that common fragile sites are hot spots not only for chromosomal lesions such as gaps but also for SCE formation.</description><identifier>ISSN: 0002-9297</identifier><identifier>EISSN: 1537-6605</identifier><identifier>PMID: 3674017</identifier><identifier>CODEN: AJHGAG</identifier><language>eng</language><publisher>Chicago, IL: University of Chicago Press</publisher><subject>Applied sciences ; Cells, Cultured ; Chromosome Fragile Sites ; Chromosome Fragility ; Chromosomes, Human, Pair 16 ; Chromosomes, Human, Pair 3 ; Exact sciences and technology ; Humans ; Karyotyping ; Lymphocytes - ultrastructure ; Other techniques and industries ; Sister Chromatid Exchange</subject><ispartof>American journal of human genetics, 1987-11, Vol.41 (5), p.882-890</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1684333/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1684333/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7072490$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3674017$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GLOVER, T. W</creatorcontrib><creatorcontrib>STEIN, C. K</creatorcontrib><title>Induction of sister chromatid exchanges at common fragile sites</title><title>American journal of human genetics</title><addtitle>Am J Hum Genet</addtitle><description>Experiments were performed to gain further insight into chromosome structure and behavior at common fragile sites by testing the hypothesis that gaps at these sites predispose to intrachromosomal recombination as measured by sister chromatid exchanges (SCEs). Human lymphocytes were concurrently treated with aphidicolin, for determination of fragile site expression, and with 5-bromodeoxy-uridine, for SCE analysis. Aphidicolin induced chromosome gaps nonrandomly, with the great majority of gaps occurring at common fragile sites. On average, 66% of gaps were accompanied by an SCE at the site of the lesion. Analysis of two specific common fragile sites at 3p14 and 16q23 showed the same pattern; that is, on average 70% of gaps at these sites were accompanied by an SCE. These results show that common fragile sites are hot spots not only for chromosomal lesions such as gaps but also for SCE formation.</description><subject>Applied sciences</subject><subject>Cells, Cultured</subject><subject>Chromosome Fragile Sites</subject><subject>Chromosome Fragility</subject><subject>Chromosomes, Human, Pair 16</subject><subject>Chromosomes, Human, Pair 3</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Karyotyping</subject><subject>Lymphocytes - ultrastructure</subject><subject>Other techniques and industries</subject><subject>Sister Chromatid Exchange</subject><issn>0002-9297</issn><issn>1537-6605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFKxDAQhoMo67r6CEIP4q0wadomuSiyuLqw4EXPZZpOdyNtszap6NtbsSx6ci4z8H38DP8Rm_NMyDjPITtmcwBIYp1oecrOvH8F4FyBmLGZyGUKXM7Z7bqrBhOs6yJXR976QH1kdr1rMdgqog-zw25LPsIQGde2o1f3uLUNjXIgf85Oamw8XUx7wV5W98_Lx3jz9LBe3m3ivUh4iPMkU6LEqgTCioNUtc6kqFOttRRUCsyzLNVGplUGqIQcL9I1oUj0N1BiwW5-cvdD2VJlqAs9NsW-ty32n4VDW_wlnd0VW_de8FylYpwFu54Cevc2kA9Fa72hpsGO3OALxUFLDvCvyFOlpJD5KF7-funwy9TtyK8mjt5gM9bWGesPmgSZpBrEF3_Eg1I</recordid><startdate>19871101</startdate><enddate>19871101</enddate><creator>GLOVER, T. W</creator><creator>STEIN, C. K</creator><general>University of Chicago Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19871101</creationdate><title>Induction of sister chromatid exchanges at common fragile sites</title><author>GLOVER, T. W ; STEIN, C. K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p321t-62583badb0ead1078f9573f499973eb3a65549c74d50a837c74e9fea329554983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Applied sciences</topic><topic>Cells, Cultured</topic><topic>Chromosome Fragile Sites</topic><topic>Chromosome Fragility</topic><topic>Chromosomes, Human, Pair 16</topic><topic>Chromosomes, Human, Pair 3</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Karyotyping</topic><topic>Lymphocytes - ultrastructure</topic><topic>Other techniques and industries</topic><topic>Sister Chromatid Exchange</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GLOVER, T. W</creatorcontrib><creatorcontrib>STEIN, C. K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>American journal of human genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GLOVER, T. W</au><au>STEIN, C. K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of sister chromatid exchanges at common fragile sites</atitle><jtitle>American journal of human genetics</jtitle><addtitle>Am J Hum Genet</addtitle><date>1987-11-01</date><risdate>1987</risdate><volume>41</volume><issue>5</issue><spage>882</spage><epage>890</epage><pages>882-890</pages><issn>0002-9297</issn><eissn>1537-6605</eissn><coden>AJHGAG</coden><abstract>Experiments were performed to gain further insight into chromosome structure and behavior at common fragile sites by testing the hypothesis that gaps at these sites predispose to intrachromosomal recombination as measured by sister chromatid exchanges (SCEs). Human lymphocytes were concurrently treated with aphidicolin, for determination of fragile site expression, and with 5-bromodeoxy-uridine, for SCE analysis. Aphidicolin induced chromosome gaps nonrandomly, with the great majority of gaps occurring at common fragile sites. On average, 66% of gaps were accompanied by an SCE at the site of the lesion. Analysis of two specific common fragile sites at 3p14 and 16q23 showed the same pattern; that is, on average 70% of gaps at these sites were accompanied by an SCE. These results show that common fragile sites are hot spots not only for chromosomal lesions such as gaps but also for SCE formation.</abstract><cop>Chicago, IL</cop><pub>University of Chicago Press</pub><pmid>3674017</pmid><tpages>9</tpages></addata></record> |
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| ispartof | American journal of human genetics, 1987-11, Vol.41 (5), p.882-890 |
| issn | 0002-9297 1537-6605 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Applied sciences Cells, Cultured Chromosome Fragile Sites Chromosome Fragility Chromosomes, Human, Pair 16 Chromosomes, Human, Pair 3 Exact sciences and technology Humans Karyotyping Lymphocytes - ultrastructure Other techniques and industries Sister Chromatid Exchange |
| title | Induction of sister chromatid exchanges at common fragile sites |
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