Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP)
We have used a recombinant DNA library constructed from flow-sorted human chromosome 8 as a source of single-copy human probes. These probes have been screened for restriction fragment length polymorphism (RFLP) by hybridization to Southern transfers of genomic DNA from five unrelated individuals. W...
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| Veröffentlicht in: | American journal of human genetics 1986-12, Vol.39 (6), p.744-750 |
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| container_title | American journal of human genetics |
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| creator | WOOD, S STARR, T. V SHUKIN, R. J |
| description | We have used a recombinant DNA library constructed from flow-sorted human chromosome 8 as a source of single-copy human probes. These probes have been screened for restriction fragment length polymorphism (RFLP) by hybridization to Southern transfers of genomic DNA from five unrelated individuals. We have detected six RFLPs distributed among four probes after screening 741 base pairs for restriction site variation. These RFLPs all behave as codominant Mendelian alleles. Two of the probes detect rare variants, while the other two detect RFLPs with PIC values of .36 and .16. Informative probes will be useful for the construction of a linkage map for chromosome 8 and for the localization of mutant alleles to this chromosome. |
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V ; SHUKIN, R. J</creator><creatorcontrib>WOOD, S ; STARR, T. V ; SHUKIN, R. J</creatorcontrib><description>We have used a recombinant DNA library constructed from flow-sorted human chromosome 8 as a source of single-copy human probes. These probes have been screened for restriction fragment length polymorphism (RFLP) by hybridization to Southern transfers of genomic DNA from five unrelated individuals. We have detected six RFLPs distributed among four probes after screening 741 base pairs for restriction site variation. These RFLPs all behave as codominant Mendelian alleles. Two of the probes detect rare variants, while the other two detect RFLPs with PIC values of .36 and .16. Informative probes will be useful for the construction of a linkage map for chromosome 8 and for the localization of mutant alleles to this chromosome.</description><identifier>ISSN: 0002-9297</identifier><identifier>EISSN: 1537-6605</identifier><identifier>PMID: 2879441</identifier><identifier>CODEN: AJHGAG</identifier><language>eng</language><publisher>Chicago, IL: University of Chicago Press</publisher><subject>Biological and medical sciences ; Chromatin. Chromosome ; Chromosomes, Human, Pair 8 ; DNA, Recombinant - isolation & purification ; Female ; Fundamental and applied biological sciences. Psychology ; Genetic Linkage ; Genetic Markers ; Humans ; Male ; Molecular and cellular biology ; Molecular genetics ; Nucleic Acid Hybridization ; Pedigree ; Polymorphism, Genetic ; Polymorphism, Restriction Fragment Length ; Repetitive Sequences, Nucleic Acid</subject><ispartof>American journal of human genetics, 1986-12, Vol.39 (6), p.744-750</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1684124/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1684124/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8367353$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2879441$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>WOOD, S</creatorcontrib><creatorcontrib>STARR, T. V</creatorcontrib><creatorcontrib>SHUKIN, R. J</creatorcontrib><title>Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP)</title><title>American journal of human genetics</title><addtitle>Am J Hum Genet</addtitle><description>We have used a recombinant DNA library constructed from flow-sorted human chromosome 8 as a source of single-copy human probes. These probes have been screened for restriction fragment length polymorphism (RFLP) by hybridization to Southern transfers of genomic DNA from five unrelated individuals. We have detected six RFLPs distributed among four probes after screening 741 base pairs for restriction site variation. These RFLPs all behave as codominant Mendelian alleles. Two of the probes detect rare variants, while the other two detect RFLPs with PIC values of .36 and .16. Informative probes will be useful for the construction of a linkage map for chromosome 8 and for the localization of mutant alleles to this chromosome.</description><subject>Biological and medical sciences</subject><subject>Chromatin. Chromosome</subject><subject>Chromosomes, Human, Pair 8</subject><subject>DNA, Recombinant - isolation & purification</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Linkage</subject><subject>Genetic Markers</subject><subject>Humans</subject><subject>Male</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Nucleic Acid Hybridization</subject><subject>Pedigree</subject><subject>Polymorphism, Genetic</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Repetitive Sequences, Nucleic Acid</subject><issn>0002-9297</issn><issn>1537-6605</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUV1rFTEQXUSpt9WfIOShiH1YyNcm2RehVFsLlyqlPi9zs8ndSLLZJrmV-kP6ew32UuxTX2ZgzpnDOTOvmhXpmGyFwN3rZoUxpm1Pe_m2Ocz5F8aEKMwOmgOqZM85WTUPlzl6KC7OCOYR6QkS6GKS-_M4jBZ9uTpFS4obk5FNMSBA1sffbY6pmBFNuwBz3atIzDEYpJB3mwTpHpUJChpNMbqgZHJJTv_TtAm2wcwFeTNvy4SW6O9DTMvkckCfrs_XP07eNW8s-Gze7_tR8_P8683Zt3b9_eLy7HTdLoyS0lowGLQkAhPb2ZHKkdKeCSEU1ng0tlOKjYpgKwHX2jNtlLGcc7HpGdOEHTWfH3WX3SaYUVdXCfywJBdqgiGCG54js5uGbbwbiFCcUF4FPu4FUrzd1ZBDcFkb72E2cZcHKSlTnHYvEgnvMe4JrcQP_1t68rJ_WcWP9zhkDb4ec9YuP9EUE5J1jP0FsKOjzA</recordid><startdate>19861201</startdate><enddate>19861201</enddate><creator>WOOD, S</creator><creator>STARR, T. V</creator><creator>SHUKIN, R. J</creator><general>University of Chicago Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19861201</creationdate><title>Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP)</title><author>WOOD, S ; STARR, T. V ; SHUKIN, R. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p321t-fae0ac71601f5fd27d229366680c0def5883d810f7a010f93ce8ef4446b933c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Biological and medical sciences</topic><topic>Chromatin. Chromosome</topic><topic>Chromosomes, Human, Pair 8</topic><topic>DNA, Recombinant - isolation & purification</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Linkage</topic><topic>Genetic Markers</topic><topic>Humans</topic><topic>Male</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nucleic Acid Hybridization</topic><topic>Pedigree</topic><topic>Polymorphism, Genetic</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Repetitive Sequences, Nucleic Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WOOD, S</creatorcontrib><creatorcontrib>STARR, T. V</creatorcontrib><creatorcontrib>SHUKIN, R. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>American journal of human genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WOOD, S</au><au>STARR, T. V</au><au>SHUKIN, R. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP)</atitle><jtitle>American journal of human genetics</jtitle><addtitle>Am J Hum Genet</addtitle><date>1986-12-01</date><risdate>1986</risdate><volume>39</volume><issue>6</issue><spage>744</spage><epage>750</epage><pages>744-750</pages><issn>0002-9297</issn><eissn>1537-6605</eissn><coden>AJHGAG</coden><abstract>We have used a recombinant DNA library constructed from flow-sorted human chromosome 8 as a source of single-copy human probes. These probes have been screened for restriction fragment length polymorphism (RFLP) by hybridization to Southern transfers of genomic DNA from five unrelated individuals. We have detected six RFLPs distributed among four probes after screening 741 base pairs for restriction site variation. These RFLPs all behave as codominant Mendelian alleles. Two of the probes detect rare variants, while the other two detect RFLPs with PIC values of .36 and .16. Informative probes will be useful for the construction of a linkage map for chromosome 8 and for the localization of mutant alleles to this chromosome.</abstract><cop>Chicago, IL</cop><pub>University of Chicago Press</pub><pmid>2879441</pmid><tpages>7</tpages></addata></record> |
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| ispartof | American journal of human genetics, 1986-12, Vol.39 (6), p.744-750 |
| issn | 0002-9297 1537-6605 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1684124 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Biological and medical sciences Chromatin. Chromosome Chromosomes, Human, Pair 8 DNA, Recombinant - isolation & purification Female Fundamental and applied biological sciences. Psychology Genetic Linkage Genetic Markers Humans Male Molecular and cellular biology Molecular genetics Nucleic Acid Hybridization Pedigree Polymorphism, Genetic Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid |
| title | Isolation and characterization of DNA probes from a flow-sorted human chromosome 8 library that detect restriction fragment length polymorphism (RFLP) |
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