Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate
Strain Co23, an anaerobic spore-forming microorganism, was enriched and isolated from a compost soil on the basis of its ability to grow with 2,3-dichlorophenol (DCP) as its electron acceptor. ortho chlorines were removed from polysubstituted phenols but not from monohalophenols. Growth by chlorores...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1996-10, Vol.62 (10), p.3800-3808 |
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| creator | Sanford, R.A. (University of Washington, Seattle, WA.) Cole, J.R Loffler, F.E Tiedje, J.M |
| description | Strain Co23, an anaerobic spore-forming microorganism, was enriched and isolated from a compost soil on the basis of its ability to grow with 2,3-dichlorophenol (DCP) as its electron acceptor. ortho chlorines were removed from polysubstituted phenols but not from monohalophenols. Growth by chlororespiration was indicated by a growth yield of 3.24 g of cells per mol of reducing equivalents (as 2[H]) from lactate oxidation to acetate in the presence of 3-chloro-4-hydroxybenzoate but no growth in the absence of the halogenated electron acceptor. Other indicators of chlororespiration were the fraction of electrons from the electron donor used for dechlorination (0.67) and the H2 threshold concentration of 1.0 ppm. Additional electron donors utilized for reductive dehalogenation were pyruvate, formate, butyrate, crotonate, and H2. Pyruvate supported homoacetogenic growth in the absence of an electron acceptor. Strain Co23 also used sulfite, thiosulfate, and sulfur as electron acceptors for growth, but it did not use sulfate, nitrate or fumarate. The temperature optimum for growth was 37 degrees C; however, the rates of dechlorination were optimum at 45 degrees C and activity persisted to temperatures as high as 55 degrees C. The 16S rRNA sequence was determined, and strain Co23 was found to be related to Desulfitobacterium dehalogenans JW/IU DC1 and Desulfitobacterium strain PCE1, with sequence similarities of 97.2 and 96.8%, respectively. The phylogenetic and physiological properties exhibited by strain Co23 place it into a new species designated Desulfitobacterium chlororespirans |
| doi_str_mv | 10.1128/aem.62.10.3800-3808.1996 |
| format | Article |
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(University of Washington, Seattle, WA.) ; Cole, J.R ; Loffler, F.E ; Tiedje, J.M</creator><creatorcontrib>Sanford, R.A. (University of Washington, Seattle, WA.) ; Cole, J.R ; Loffler, F.E ; Tiedje, J.M</creatorcontrib><description>Strain Co23, an anaerobic spore-forming microorganism, was enriched and isolated from a compost soil on the basis of its ability to grow with 2,3-dichlorophenol (DCP) as its electron acceptor. ortho chlorines were removed from polysubstituted phenols but not from monohalophenols. Growth by chlororespiration was indicated by a growth yield of 3.24 g of cells per mol of reducing equivalents (as 2[H]) from lactate oxidation to acetate in the presence of 3-chloro-4-hydroxybenzoate but no growth in the absence of the halogenated electron acceptor. Other indicators of chlororespiration were the fraction of electrons from the electron donor used for dechlorination (0.67) and the H2 threshold concentration of 1.0 ppm. Additional electron donors utilized for reductive dehalogenation were pyruvate, formate, butyrate, crotonate, and H2. Pyruvate supported homoacetogenic growth in the absence of an electron acceptor. Strain Co23 also used sulfite, thiosulfate, and sulfur as electron acceptors for growth, but it did not use sulfate, nitrate or fumarate. The temperature optimum for growth was 37 degrees C; however, the rates of dechlorination were optimum at 45 degrees C and activity persisted to temperatures as high as 55 degrees C. The 16S rRNA sequence was determined, and strain Co23 was found to be related to Desulfitobacterium dehalogenans JW/IU DC1 and Desulfitobacterium strain PCE1, with sequence similarities of 97.2 and 96.8%, respectively. The phylogenetic and physiological properties exhibited by strain Co23 place it into a new species designated Desulfitobacterium chlororespirans</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/aem.62.10.3800-3808.1996</identifier><identifier>PMID: 8837437</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>ACIDE BENZOIQUE ; ACIDE LACTIQUE ; ACIDE ORGANIQUE ; ACIDO BENZOICO ; ACIDO LACTICO ; ACIDOS ORGANICOS ; ANAEROBIOSE ; ANAEROBIOSIS ; BACTERIA ; BACTERIA GRAM POSITIVA ; Bacteria, Anaerobic - genetics ; Bacteria, Anaerobic - growth & development ; Bacteria, Anaerobic - isolation & purification ; Bacteria, Anaerobic - metabolism ; BACTERIE GRAM POSITIF ; BIODEGRADACION ; BIODEGRADATION ; Biodegradation, Environmental ; Biological and medical sciences ; Biology of microorganisms of confirmed or potential industrial interest ; Biotechnology ; CHIMIOTAXONOMIE ; CHLORE ; Chlorine - metabolism ; Chlorobenzoates ; CLORO ; COMPOSE ORGANOCHLORE ; COMPUESTO ORGANICO DEL CLORO ; Desulfitobacterium chlororespirans ; ESPECE NOUVELLE ; ESPECIES NUEVAS ; FLORA DEL SUELO ; FLORE DU SOL ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Hydroxybenzoates - metabolism ; Isolation and description ; Lactic Acid - metabolism ; Microbiology ; MICROORGANISME ; MICROORGANISMOS ; Microorganisms ; Mission oriented research ; Molecular Sequence Data ; OXIDACION ; Oxidation ; Oxidation-Reduction ; OXYDATION ; Phylogeny ; Pyruvic Acid - metabolism ; QUIMIOTAXONOMIA ; REDUCCION ; REDUCTION ; RNA, Ribosomal, 16S - genetics ; SECUENCIA NUCLEOTIDICA ; Sequence Homology, Nucleic Acid ; SEQUENCE NUCLEOTIDIQUE ; Soil Microbiology ; Soils ; Spores, Bacterial - isolation & purification ; Substrate Specificity ; Temperature</subject><ispartof>Applied and Environmental Microbiology, 1996-10, Vol.62 (10), p.3800-3808</ispartof><rights>1997 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Oct 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c668t-e4c2d9fb1139a937e64f502f2a931fbcfe7991ce36be449fb2e1b276b69997593</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC168189/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC168189/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,728,781,785,886,3189,3190,27929,27930,53796,53798</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2474556$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8837437$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sanford, R.A. (University of Washington, Seattle, WA.)</creatorcontrib><creatorcontrib>Cole, J.R</creatorcontrib><creatorcontrib>Loffler, F.E</creatorcontrib><creatorcontrib>Tiedje, J.M</creatorcontrib><title>Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Strain Co23, an anaerobic spore-forming microorganism, was enriched and isolated from a compost soil on the basis of its ability to grow with 2,3-dichlorophenol (DCP) as its electron acceptor. ortho chlorines were removed from polysubstituted phenols but not from monohalophenols. Growth by chlororespiration was indicated by a growth yield of 3.24 g of cells per mol of reducing equivalents (as 2[H]) from lactate oxidation to acetate in the presence of 3-chloro-4-hydroxybenzoate but no growth in the absence of the halogenated electron acceptor. Other indicators of chlororespiration were the fraction of electrons from the electron donor used for dechlorination (0.67) and the H2 threshold concentration of 1.0 ppm. Additional electron donors utilized for reductive dehalogenation were pyruvate, formate, butyrate, crotonate, and H2. Pyruvate supported homoacetogenic growth in the absence of an electron acceptor. Strain Co23 also used sulfite, thiosulfate, and sulfur as electron acceptors for growth, but it did not use sulfate, nitrate or fumarate. The temperature optimum for growth was 37 degrees C; however, the rates of dechlorination were optimum at 45 degrees C and activity persisted to temperatures as high as 55 degrees C. The 16S rRNA sequence was determined, and strain Co23 was found to be related to Desulfitobacterium dehalogenans JW/IU DC1 and Desulfitobacterium strain PCE1, with sequence similarities of 97.2 and 96.8%, respectively. The phylogenetic and physiological properties exhibited by strain Co23 place it into a new species designated Desulfitobacterium chlororespirans</description><subject>ACIDE BENZOIQUE</subject><subject>ACIDE LACTIQUE</subject><subject>ACIDE ORGANIQUE</subject><subject>ACIDO BENZOICO</subject><subject>ACIDO LACTICO</subject><subject>ACIDOS ORGANICOS</subject><subject>ANAEROBIOSE</subject><subject>ANAEROBIOSIS</subject><subject>BACTERIA</subject><subject>BACTERIA GRAM POSITIVA</subject><subject>Bacteria, Anaerobic - genetics</subject><subject>Bacteria, Anaerobic - growth & development</subject><subject>Bacteria, Anaerobic - isolation & purification</subject><subject>Bacteria, Anaerobic - metabolism</subject><subject>BACTERIE GRAM POSITIF</subject><subject>BIODEGRADACION</subject><subject>BIODEGRADATION</subject><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>Biology of microorganisms of confirmed or potential industrial interest</subject><subject>Biotechnology</subject><subject>CHIMIOTAXONOMIE</subject><subject>CHLORE</subject><subject>Chlorine - metabolism</subject><subject>Chlorobenzoates</subject><subject>CLORO</subject><subject>COMPOSE ORGANOCHLORE</subject><subject>COMPUESTO ORGANICO DEL CLORO</subject><subject>Desulfitobacterium chlororespirans</subject><subject>ESPECE NOUVELLE</subject><subject>ESPECIES NUEVAS</subject><subject>FLORA DEL SUELO</subject><subject>FLORE DU SOL</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydroxybenzoates - metabolism</subject><subject>Isolation and description</subject><subject>Lactic Acid - metabolism</subject><subject>Microbiology</subject><subject>MICROORGANISME</subject><subject>MICROORGANISMOS</subject><subject>Microorganisms</subject><subject>Mission oriented research</subject><subject>Molecular Sequence Data</subject><subject>OXIDACION</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>OXYDATION</subject><subject>Phylogeny</subject><subject>Pyruvic Acid - metabolism</subject><subject>QUIMIOTAXONOMIA</subject><subject>REDUCCION</subject><subject>REDUCTION</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>Soil Microbiology</subject><subject>Soils</subject><subject>Spores, Bacterial - isolation & purification</subject><subject>Substrate Specificity</subject><subject>Temperature</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdks1u1DAUhSMEKqXwAkhIFkKsSPBP4tiLLtDwK1ViAV1bjnM9cZXEg53MdPpSvCJOZzRAN46uznfOtZWTZYjgghAq3msYCk6LNDKBcZ4OURAp-aPsnGAp8oox_jg7x1jKnNISP82exXiDMS4xF2fZmRCsLll9nv1edTpoM0Fwd3pyfkTeoo8Q5966yTcHZR6Q6XoffIC4cUGPEcVNgUa_Ld6hXedMh9bB7yJq9sj4edO7cY2mDpC_de0ptU9hegI0-XstQDubyW0BtXCf7sYTyvLDvrzMu30b_O2-gfHOJ_fz7InVfYQXx-9Fdv3508_V1_zq-5dvqw9XueFcTDmUhrbSNoQwqSWrgZe2wtTSNBDbGAu1lMQA4w2UZQIpkIbWvOFSyrqS7CK7PORu5maA1sA4Bd2rTXCDDnvltVP_K6Pr1NpvFeGCiMX_9ugP_tcMcVKDiwb6Xo_g56hIVdfLngS-fgDe-DmM6W2K4gTQUvAEiQNkgo8xgD1dhGC1FEKlQihOl3EpxHIItRQiWV_9-5CT8diApL856joa3dv0d42LJ4yWdVlV_O81O7fudi6A0nF4sDVBLw-Q1V7pdUg51z9kjSlnlP0BGHvYbQ</recordid><startdate>19961001</startdate><enddate>19961001</enddate><creator>Sanford, R.A. (University of Washington, Seattle, WA.)</creator><creator>Cole, J.R</creator><creator>Loffler, F.E</creator><creator>Tiedje, J.M</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>5PM</scope></search><sort><creationdate>19961001</creationdate><title>Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate</title><author>Sanford, R.A. (University of Washington, Seattle, WA.) ; Cole, J.R ; Loffler, F.E ; Tiedje, J.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c668t-e4c2d9fb1139a937e64f502f2a931fbcfe7991ce36be449fb2e1b276b69997593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ACIDE BENZOIQUE</topic><topic>ACIDE LACTIQUE</topic><topic>ACIDE ORGANIQUE</topic><topic>ACIDO BENZOICO</topic><topic>ACIDO LACTICO</topic><topic>ACIDOS ORGANICOS</topic><topic>ANAEROBIOSE</topic><topic>ANAEROBIOSIS</topic><topic>BACTERIA</topic><topic>BACTERIA GRAM POSITIVA</topic><topic>Bacteria, Anaerobic - genetics</topic><topic>Bacteria, Anaerobic - growth & development</topic><topic>Bacteria, Anaerobic - isolation & purification</topic><topic>Bacteria, Anaerobic - metabolism</topic><topic>BACTERIE GRAM POSITIF</topic><topic>BIODEGRADACION</topic><topic>BIODEGRADATION</topic><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>Biology of microorganisms of confirmed or potential industrial interest</topic><topic>Biotechnology</topic><topic>CHIMIOTAXONOMIE</topic><topic>CHLORE</topic><topic>Chlorine - metabolism</topic><topic>Chlorobenzoates</topic><topic>CLORO</topic><topic>COMPOSE ORGANOCHLORE</topic><topic>COMPUESTO ORGANICO DEL CLORO</topic><topic>Desulfitobacterium chlororespirans</topic><topic>ESPECE NOUVELLE</topic><topic>ESPECIES NUEVAS</topic><topic>FLORA DEL SUELO</topic><topic>FLORE DU SOL</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydroxybenzoates - metabolism</topic><topic>Isolation and description</topic><topic>Lactic Acid - metabolism</topic><topic>Microbiology</topic><topic>MICROORGANISME</topic><topic>MICROORGANISMOS</topic><topic>Microorganisms</topic><topic>Mission oriented research</topic><topic>Molecular Sequence Data</topic><topic>OXIDACION</topic><topic>Oxidation</topic><topic>Oxidation-Reduction</topic><topic>OXYDATION</topic><topic>Phylogeny</topic><topic>Pyruvic Acid - metabolism</topic><topic>QUIMIOTAXONOMIA</topic><topic>REDUCCION</topic><topic>REDUCTION</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>Soil Microbiology</topic><topic>Soils</topic><topic>Spores, Bacterial - isolation & purification</topic><topic>Substrate Specificity</topic><topic>Temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sanford, R.A. (University of Washington, Seattle, WA.)</creatorcontrib><creatorcontrib>Cole, J.R</creatorcontrib><creatorcontrib>Loffler, F.E</creatorcontrib><creatorcontrib>Tiedje, J.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sanford, R.A. (University of Washington, Seattle, WA.)</au><au>Cole, J.R</au><au>Loffler, F.E</au><au>Tiedje, J.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1996-10-01</date><risdate>1996</risdate><volume>62</volume><issue>10</issue><spage>3800</spage><epage>3808</epage><pages>3800-3808</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Strain Co23, an anaerobic spore-forming microorganism, was enriched and isolated from a compost soil on the basis of its ability to grow with 2,3-dichlorophenol (DCP) as its electron acceptor. ortho chlorines were removed from polysubstituted phenols but not from monohalophenols. Growth by chlororespiration was indicated by a growth yield of 3.24 g of cells per mol of reducing equivalents (as 2[H]) from lactate oxidation to acetate in the presence of 3-chloro-4-hydroxybenzoate but no growth in the absence of the halogenated electron acceptor. Other indicators of chlororespiration were the fraction of electrons from the electron donor used for dechlorination (0.67) and the H2 threshold concentration of 1.0 ppm. Additional electron donors utilized for reductive dehalogenation were pyruvate, formate, butyrate, crotonate, and H2. Pyruvate supported homoacetogenic growth in the absence of an electron acceptor. Strain Co23 also used sulfite, thiosulfate, and sulfur as electron acceptors for growth, but it did not use sulfate, nitrate or fumarate. The temperature optimum for growth was 37 degrees C; however, the rates of dechlorination were optimum at 45 degrees C and activity persisted to temperatures as high as 55 degrees C. The 16S rRNA sequence was determined, and strain Co23 was found to be related to Desulfitobacterium dehalogenans JW/IU DC1 and Desulfitobacterium strain PCE1, with sequence similarities of 97.2 and 96.8%, respectively. The phylogenetic and physiological properties exhibited by strain Co23 place it into a new species designated Desulfitobacterium chlororespirans</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8837437</pmid><doi>10.1128/aem.62.10.3800-3808.1996</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 1996-10, Vol.62 (10), p.3800-3808 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_168189 |
| source | American Society for Microbiology; MEDLINE; PubMed Central; Alma/SFX Local Collection |
| subjects | ACIDE BENZOIQUE ACIDE LACTIQUE ACIDE ORGANIQUE ACIDO BENZOICO ACIDO LACTICO ACIDOS ORGANICOS ANAEROBIOSE ANAEROBIOSIS BACTERIA BACTERIA GRAM POSITIVA Bacteria, Anaerobic - genetics Bacteria, Anaerobic - growth & development Bacteria, Anaerobic - isolation & purification Bacteria, Anaerobic - metabolism BACTERIE GRAM POSITIF BIODEGRADACION BIODEGRADATION Biodegradation, Environmental Biological and medical sciences Biology of microorganisms of confirmed or potential industrial interest Biotechnology CHIMIOTAXONOMIE CHLORE Chlorine - metabolism Chlorobenzoates CLORO COMPOSE ORGANOCHLORE COMPUESTO ORGANICO DEL CLORO Desulfitobacterium chlororespirans ESPECE NOUVELLE ESPECIES NUEVAS FLORA DEL SUELO FLORE DU SOL Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Hydroxybenzoates - metabolism Isolation and description Lactic Acid - metabolism Microbiology MICROORGANISME MICROORGANISMOS Microorganisms Mission oriented research Molecular Sequence Data OXIDACION Oxidation Oxidation-Reduction OXYDATION Phylogeny Pyruvic Acid - metabolism QUIMIOTAXONOMIA REDUCCION REDUCTION RNA, Ribosomal, 16S - genetics SECUENCIA NUCLEOTIDICA Sequence Homology, Nucleic Acid SEQUENCE NUCLEOTIDIQUE Soil Microbiology Soils Spores, Bacterial - isolation & purification Substrate Specificity Temperature |
| title | Characterization of Desulfitobacterium chlororespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate |
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