Natural transmission of Salmonella choleraesuis in swine
This experiment was designed to study the natural transmission of Salmonella choleraesuis in swine. Forty pigs were divided into three groups. Group 1 (n = 12) was challenged with 10(8) CFU of S. choleraesuis per ml by intranasal inoculation. One day postinoculation (p.i.), group 2 (n = 24) was comm...
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| Veröffentlicht in: | Applied and Environmental Microbiology 1996, Vol.62 (1), p.141-146 |
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| description | This experiment was designed to study the natural transmission of Salmonella choleraesuis in swine. Forty pigs were divided into three groups. Group 1 (n = 12) was challenged with 10(8) CFU of S. choleraesuis per ml by intranasal inoculation. One day postinoculation (p.i.), group 2 (n = 24) was commingled with group 1. Group 3 (n = 4) served as uninoculated controls. Serum samples were collected weekly. Blastogenesis assays and necropsies were performed at 1, 2, 4, 6, 9, and 12 weeks p.i., and 16 tissue samples per pig were collected and cultured. Environmental (pooled feces from the pen floor) levels of S. choleraesuis were 2.61 log10 CFU/g of feces at 24 h p.i. (immediately prior to commingling). Severe clinical signs were observed in groups 1 and 2. The results indicated that at least 16% of group 2 pigs were shedding S. choleraesuis within 24 h of commingling. At 1 week p.i., 32 of 32 group 1 and 39 of 62 group 2 tissue samples were positive for S. choleraesuis. Only 3 of 12 group 2 pigs were positive at 6, 9, and 12 weeks (1 pig for each week), indicating that only a small proportion of infected swine become long-term carriers. At 12 weeks p.i., only the colon and colonic lymph node samples of one pig from group 2 were positive. Humoral, mucosal, and cellular immune responses were similar between groups 1 and 2. These data demonstrate that a few pigs shedding low levels of Salmonella organisms before slaughter can result in rapid transmission and subsequent shedding by many swine. |
| doi_str_mv | 10.1128/AEM.62.1.141-146.1996 |
| format | Article |
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T ; FEDORKA-CRAY, P. J ; STABEL, T. J ; KRAMER, T. T</creator><creatorcontrib>GRAY, J. T ; FEDORKA-CRAY, P. J ; STABEL, T. J ; KRAMER, T. T ; University of Osteopathic Medicine and Health Sciences, Des Moines, IA ; Society of Estonian Veterinarians, Tartu (Estonia)</creatorcontrib><description>This experiment was designed to study the natural transmission of Salmonella choleraesuis in swine. Forty pigs were divided into three groups. Group 1 (n = 12) was challenged with 10(8) CFU of S. choleraesuis per ml by intranasal inoculation. One day postinoculation (p.i.), group 2 (n = 24) was commingled with group 1. Group 3 (n = 4) served as uninoculated controls. Serum samples were collected weekly. Blastogenesis assays and necropsies were performed at 1, 2, 4, 6, 9, and 12 weeks p.i., and 16 tissue samples per pig were collected and cultured. Environmental (pooled feces from the pen floor) levels of S. choleraesuis were 2.61 log10 CFU/g of feces at 24 h p.i. (immediately prior to commingling). Severe clinical signs were observed in groups 1 and 2. The results indicated that at least 16% of group 2 pigs were shedding S. choleraesuis within 24 h of commingling. At 1 week p.i., 32 of 32 group 1 and 39 of 62 group 2 tissue samples were positive for S. choleraesuis. Only 3 of 12 group 2 pigs were positive at 6, 9, and 12 weeks (1 pig for each week), indicating that only a small proportion of infected swine become long-term carriers. At 12 weeks p.i., only the colon and colonic lymph node samples of one pig from group 2 were positive. Humoral, mucosal, and cellular immune responses were similar between groups 1 and 2. These data demonstrate that a few pigs shedding low levels of Salmonella organisms before slaughter can result in rapid transmission and subsequent shedding by many swine.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.62.1.141-146.1996</identifier><identifier>PMID: 8572691</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>animal tissues ; Animals ; Antibodies, Bacterial - analysis ; Antibodies, Bacterial - blood ; Antigens, Bacterial - immunology ; Bacteria ; Bacteriology ; Biological and medical sciences ; Biology ; body parts ; Carrier State - veterinary ; cell mediated immunity ; cerdo ; disease transmission ; Feces - microbiology ; Fundamental and applied biological sciences. Psychology ; Hogs ; immune response ; immunite cellulaire ; inmunidad celular ; Intestinal Mucosa - immunology ; Lymphocyte Activation ; Microbiology ; partes del cuerpo ; partie du corps ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; porcin ; reponse immunitaire ; respuesta inmunologica ; Salmonella ; Salmonella - immunology ; Salmonella Infections, Animal - immunology ; Salmonella Infections, Animal - microbiology ; Salmonella Infections, Animal - transmission ; salmonellose ; salmonellosis ; salmonelosis ; sintomas ; Swine ; Swine Diseases - immunology ; Swine Diseases - microbiology ; Swine Diseases - transmission ; symptome ; symptoms ; tejidos animales ; tissu animal ; transmision de enfermedades ; transmission des maladies</subject><ispartof>Applied and Environmental Microbiology, 1996, Vol.62 (1), p.141-146</ispartof><rights>1996 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Jan 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c592t-266cb6433c1703e5201f460042f1d18f3c26acc31c49b7809c964409de167aaa3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC167782/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC167782/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,4024,27923,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3103799$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8572691$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>GRAY, J. T</creatorcontrib><creatorcontrib>FEDORKA-CRAY, P. J</creatorcontrib><creatorcontrib>STABEL, T. J</creatorcontrib><creatorcontrib>KRAMER, T. T</creatorcontrib><creatorcontrib>University of Osteopathic Medicine and Health Sciences, Des Moines, IA</creatorcontrib><creatorcontrib>Society of Estonian Veterinarians, Tartu (Estonia)</creatorcontrib><title>Natural transmission of Salmonella choleraesuis in swine</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>This experiment was designed to study the natural transmission of Salmonella choleraesuis in swine. Forty pigs were divided into three groups. Group 1 (n = 12) was challenged with 10(8) CFU of S. choleraesuis per ml by intranasal inoculation. One day postinoculation (p.i.), group 2 (n = 24) was commingled with group 1. Group 3 (n = 4) served as uninoculated controls. Serum samples were collected weekly. Blastogenesis assays and necropsies were performed at 1, 2, 4, 6, 9, and 12 weeks p.i., and 16 tissue samples per pig were collected and cultured. Environmental (pooled feces from the pen floor) levels of S. choleraesuis were 2.61 log10 CFU/g of feces at 24 h p.i. (immediately prior to commingling). Severe clinical signs were observed in groups 1 and 2. The results indicated that at least 16% of group 2 pigs were shedding S. choleraesuis within 24 h of commingling. At 1 week p.i., 32 of 32 group 1 and 39 of 62 group 2 tissue samples were positive for S. choleraesuis. Only 3 of 12 group 2 pigs were positive at 6, 9, and 12 weeks (1 pig for each week), indicating that only a small proportion of infected swine become long-term carriers. At 12 weeks p.i., only the colon and colonic lymph node samples of one pig from group 2 were positive. Humoral, mucosal, and cellular immune responses were similar between groups 1 and 2. These data demonstrate that a few pigs shedding low levels of Salmonella organisms before slaughter can result in rapid transmission and subsequent shedding by many swine.</description><subject>animal tissues</subject><subject>Animals</subject><subject>Antibodies, Bacterial - analysis</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biology</subject><subject>body parts</subject><subject>Carrier State - veterinary</subject><subject>cell mediated immunity</subject><subject>cerdo</subject><subject>disease transmission</subject><subject>Feces - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hogs</subject><subject>immune response</subject><subject>immunite cellulaire</subject><subject>inmunidad celular</subject><subject>Intestinal Mucosa - immunology</subject><subject>Lymphocyte Activation</subject><subject>Microbiology</subject><subject>partes del cuerpo</subject><subject>partie du corps</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>porcin</subject><subject>reponse immunitaire</subject><subject>respuesta inmunologica</subject><subject>Salmonella</subject><subject>Salmonella - immunology</subject><subject>Salmonella Infections, Animal - immunology</subject><subject>Salmonella Infections, Animal - microbiology</subject><subject>Salmonella Infections, Animal - transmission</subject><subject>salmonellose</subject><subject>salmonellosis</subject><subject>salmonelosis</subject><subject>sintomas</subject><subject>Swine</subject><subject>Swine Diseases - immunology</subject><subject>Swine Diseases - microbiology</subject><subject>Swine Diseases - transmission</subject><subject>symptome</subject><subject>symptoms</subject><subject>tejidos animales</subject><subject>tissu animal</subject><subject>transmision de enfermedades</subject><subject>transmission des maladies</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkkFv1DAQhS0EKtvCTyiKEOotYcZ2nPjAoapKW6nAofRszXqdXVdJXOwNFf--jna1olw4WD7M98Zv5pmxU4QKkbefzy-_VYpXWKHEEqWqUGv1ii0QdFvWQqjXbAGgdcm5hLfsOKUHAJCg2iN21NYNVxoXrP1O2ylSX2wjjWnwKfkwFqEr7qgfwuj6ngq7Cb2L5NLkU-HHIj350b1jbzrqk3u_v0_Y_dfLnxfX5e2Pq5uL89vS1ppvS66UXSophMUGhKs5YCdV9sE7XGHbCcsVWSvQSr1sWtBWKylBrxyqhojECfuy6_s4LQe3sm7MTnvzGP1A8Y8J5M3Lyug3Zh1-m6xvWp71Z3t9DL8ml7YmD2nnuUYXpmSaRivecvVfMPvnQjc6gx__AR_CFMe8BMOh1hJqnLvVO8jGkFJ03cExgpnzM-QGo7hBk_PLR5k5v6w7_Xvcg2ofWK5_2tcpWeq7nJr16YAJBNHo2WOxwzZ-vXny0RlKw4snM_Jhh3QUDK1j7nJ_N3sABMhfRjwDkxO2ng</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>GRAY, J. T</creator><creator>FEDORKA-CRAY, P. J</creator><creator>STABEL, T. J</creator><creator>KRAMER, T. T</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>1996</creationdate><title>Natural transmission of Salmonella choleraesuis in swine</title><author>GRAY, J. T ; FEDORKA-CRAY, P. J ; STABEL, T. J ; KRAMER, T. T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c592t-266cb6433c1703e5201f460042f1d18f3c26acc31c49b7809c964409de167aaa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>animal tissues</topic><topic>Animals</topic><topic>Antibodies, Bacterial - analysis</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biology</topic><topic>body parts</topic><topic>Carrier State - veterinary</topic><topic>cell mediated immunity</topic><topic>cerdo</topic><topic>disease transmission</topic><topic>Feces - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hogs</topic><topic>immune response</topic><topic>immunite cellulaire</topic><topic>inmunidad celular</topic><topic>Intestinal Mucosa - immunology</topic><topic>Lymphocyte Activation</topic><topic>Microbiology</topic><topic>partes del cuerpo</topic><topic>partie du corps</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>porcin</topic><topic>reponse immunitaire</topic><topic>respuesta inmunologica</topic><topic>Salmonella</topic><topic>Salmonella - immunology</topic><topic>Salmonella Infections, Animal - immunology</topic><topic>Salmonella Infections, Animal - microbiology</topic><topic>Salmonella Infections, Animal - transmission</topic><topic>salmonellose</topic><topic>salmonellosis</topic><topic>salmonelosis</topic><topic>sintomas</topic><topic>Swine</topic><topic>Swine Diseases - immunology</topic><topic>Swine Diseases - microbiology</topic><topic>Swine Diseases - transmission</topic><topic>symptome</topic><topic>symptoms</topic><topic>tejidos animales</topic><topic>tissu animal</topic><topic>transmision de enfermedades</topic><topic>transmission des maladies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GRAY, J. T</creatorcontrib><creatorcontrib>FEDORKA-CRAY, P. J</creatorcontrib><creatorcontrib>STABEL, T. J</creatorcontrib><creatorcontrib>KRAMER, T. T</creatorcontrib><creatorcontrib>University of Osteopathic Medicine and Health Sciences, Des Moines, IA</creatorcontrib><creatorcontrib>Society of Estonian Veterinarians, Tartu (Estonia)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GRAY, J. T</au><au>FEDORKA-CRAY, P. J</au><au>STABEL, T. J</au><au>KRAMER, T. T</au><aucorp>University of Osteopathic Medicine and Health Sciences, Des Moines, IA</aucorp><aucorp>Society of Estonian Veterinarians, Tartu (Estonia)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Natural transmission of Salmonella choleraesuis in swine</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>1996</date><risdate>1996</risdate><volume>62</volume><issue>1</issue><spage>141</spage><epage>146</epage><pages>141-146</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>This experiment was designed to study the natural transmission of Salmonella choleraesuis in swine. Forty pigs were divided into three groups. Group 1 (n = 12) was challenged with 10(8) CFU of S. choleraesuis per ml by intranasal inoculation. One day postinoculation (p.i.), group 2 (n = 24) was commingled with group 1. Group 3 (n = 4) served as uninoculated controls. Serum samples were collected weekly. Blastogenesis assays and necropsies were performed at 1, 2, 4, 6, 9, and 12 weeks p.i., and 16 tissue samples per pig were collected and cultured. Environmental (pooled feces from the pen floor) levels of S. choleraesuis were 2.61 log10 CFU/g of feces at 24 h p.i. (immediately prior to commingling). Severe clinical signs were observed in groups 1 and 2. The results indicated that at least 16% of group 2 pigs were shedding S. choleraesuis within 24 h of commingling. At 1 week p.i., 32 of 32 group 1 and 39 of 62 group 2 tissue samples were positive for S. choleraesuis. Only 3 of 12 group 2 pigs were positive at 6, 9, and 12 weeks (1 pig for each week), indicating that only a small proportion of infected swine become long-term carriers. At 12 weeks p.i., only the colon and colonic lymph node samples of one pig from group 2 were positive. Humoral, mucosal, and cellular immune responses were similar between groups 1 and 2. These data demonstrate that a few pigs shedding low levels of Salmonella organisms before slaughter can result in rapid transmission and subsequent shedding by many swine.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8572691</pmid><doi>10.1128/AEM.62.1.141-146.1996</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Applied and Environmental Microbiology, 1996, Vol.62 (1), p.141-146 |
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| subjects | animal tissues Animals Antibodies, Bacterial - analysis Antibodies, Bacterial - blood Antigens, Bacterial - immunology Bacteria Bacteriology Biological and medical sciences Biology body parts Carrier State - veterinary cell mediated immunity cerdo disease transmission Feces - microbiology Fundamental and applied biological sciences. Psychology Hogs immune response immunite cellulaire inmunidad celular Intestinal Mucosa - immunology Lymphocyte Activation Microbiology partes del cuerpo partie du corps Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains porcin reponse immunitaire respuesta inmunologica Salmonella Salmonella - immunology Salmonella Infections, Animal - immunology Salmonella Infections, Animal - microbiology Salmonella Infections, Animal - transmission salmonellose salmonellosis salmonelosis sintomas Swine Swine Diseases - immunology Swine Diseases - microbiology Swine Diseases - transmission symptome symptoms tejidos animales tissu animal transmision de enfermedades transmission des maladies |
| title | Natural transmission of Salmonella choleraesuis in swine |
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