Expression of the plastid-located glutamine synthetase of Medicago truncatula. Accumulation of the precursor in root nodules reveals an in vivo control at the level of protein import into plastids

In this paper, we report the cloning and characterization of the plastid-located glutamine synthetase (GS) of Medicago truncatula Gaertn (MtGS2). A cDNA was isolated encoding a GS2 precursor polypeptide of 428 amino acids composing an N-terminal transit peptide of 49 amino acids. Expression analysis...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology (Bethesda) 2003-05, Vol.132 (1), p.390-399
Hauptverfasser:	Melo, P.M, Lima, L.M, Santos, I.M, Carvalho, H.G, Cullimore, J.V
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Alfalfa Amino Acid Sequence amino acid sequences Amino acids bioaccumulation Biological and medical sciences Blotting, Western cell fractionation Cloning Complementary DNA DNA, Complementary - chemistry DNA, Complementary - genetics DNA, Complementary - isolation & purification Economic plant physiology Enzyme Precursors - genetics Enzyme Precursors - metabolism Enzymes Escherichia coli Escherichia coli - genetics forage legumes Fractionation Fundamental and applied biological sciences. Psychology gene expression Gene expression regulation Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant glutamate-ammonia ligase Glutamate-Ammonia Ligase - genetics Glutamate-Ammonia Ligase - metabolism Medicago - enzymology Medicago - genetics Medicago - microbiology Medicago truncatula Messenger RNA Metabolism Microscopy, Immunoelectron molecular cloning Molecular Sequence Data molecular sequences data Nitrogen fixation Nitrogen metabolism Nitrogen metabolism and other ones (excepting carbon metabolism) Nodules Nutrition. Photosynthesis. Respiration. Metabolism Parasitism and symbiosis Plant physiology and development plant proteins Plant Proteins - genetics Plant Proteins - metabolism Plant roots Plant Roots - enzymology Plant Roots - microbiology Plant Roots - ultrastructure Plants Plants Interacting with Other Organisms Plastids Plastids - enzymology polypeptides Protein precursors protein transport Root nodules Sequence Analysis, DNA Sequence Homology, Amino Acid subcellular fractions Symbiosis Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) Symbiosis - genetics tissue distribution
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!