THE DISTRIBUTION AND METABOLISM OF ARACHIDONIC ACID IN RABBIT PLATELETS DURING AGGREGATION AND ITS MODIFICATION BY DRUGS
1Gas chromatographic and radio‐isotope labelling techniques have been used to establish the origin of the arachidonic acid used by the platelet cyclo‐oxygenase for the synthesis of pro‐aggregatory prostaglandin endoperoxide derivatives. 2Measurements of total platelet arachidonate content indicated...
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Veröffentlicht in: | British journal of pharmacology 1977-02, Vol.59 (2), p.353-366 |
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description | 1Gas chromatographic and radio‐isotope labelling techniques have been used to establish the origin of the arachidonic acid used by the platelet cyclo‐oxygenase for the synthesis of pro‐aggregatory prostaglandin endoperoxide derivatives.
2Measurements of total platelet arachidonate content indicated that more than 95% is esterified in the phosphatide fraction of the cells.
3During aggregation by collagen or thrombin as much as 80% of the total platelet arachidonate may be liberated and transformed by the platelet enzymes into hydroxyacids and other more polar compounds.
4The phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol fractions are major sources of the arachidonate thus used.
5Indomethacin, which prevents platelet aggregation by inhibiting the cyclo‐oxygenase, did not affect this release of arachidonate from the phosphatides but did prevent the transformation of arachidonate to endoperoxide derivatives.
6Mepacrine, a drug which possesses weak anti‐phospholipase activity in platelets, also prevents aggregation by collagen or thrombin, but seems to do so by preventing substrate release from the phosphatide fraction.
7It is suggested that phospholipase A2 plays a key role in the initial events during platelet aggregation induced by collagen. |
doi_str_mv | 10.1111/j.1476-5381.1977.tb07500.x |
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2Measurements of total platelet arachidonate content indicated that more than 95% is esterified in the phosphatide fraction of the cells.
3During aggregation by collagen or thrombin as much as 80% of the total platelet arachidonate may be liberated and transformed by the platelet enzymes into hydroxyacids and other more polar compounds.
4The phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol fractions are major sources of the arachidonate thus used.
5Indomethacin, which prevents platelet aggregation by inhibiting the cyclo‐oxygenase, did not affect this release of arachidonate from the phosphatides but did prevent the transformation of arachidonate to endoperoxide derivatives.
6Mepacrine, a drug which possesses weak anti‐phospholipase activity in platelets, also prevents aggregation by collagen or thrombin, but seems to do so by preventing substrate release from the phosphatide fraction.
7It is suggested that phospholipase A2 plays a key role in the initial events during platelet aggregation induced by collagen.</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1111/j.1476-5381.1977.tb07500.x</identifier><identifier>PMID: 837023</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Arachidonic Acids - blood ; Blood Platelets - analysis ; Blood Platelets - drug effects ; Blood Platelets - enzymology ; Blood Platelets - metabolism ; Carbon Radioisotopes ; Chemical Phenomena ; Chemistry ; Chromatography, Gas ; Collagen - pharmacology ; Fatty Acids - blood ; Hydrolysis ; In Vitro Techniques ; Indomethacin - pharmacology ; Lipids - blood ; Male ; Phosphatidylcholines - metabolism ; Phospholipases - blood ; Phospholipids - blood ; Phosphorus - blood ; Platelet Aggregation - drug effects ; Quinacrine - pharmacology ; Rabbits ; Thrombin - pharmacology</subject><ispartof>British journal of pharmacology, 1977-02, Vol.59 (2), p.353-366</ispartof><rights>1977 British Pharmacological Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4570-6c0c8171230369a02a43d65ba6cd03c43da1f199c52d680f1c912857220c3c863</citedby><cites>FETCH-LOGICAL-c4570-6c0c8171230369a02a43d65ba6cd03c43da1f199c52d680f1c912857220c3c863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1667739/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1667739/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/837023$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BLACKWELL, G.J.</creatorcontrib><creatorcontrib>DUNCOMBE, W.G.</creatorcontrib><creatorcontrib>FLOWER, R.J.</creatorcontrib><creatorcontrib>PARSONS, M.F.</creatorcontrib><creatorcontrib>VANE, J.R.</creatorcontrib><title>THE DISTRIBUTION AND METABOLISM OF ARACHIDONIC ACID IN RABBIT PLATELETS DURING AGGREGATION AND ITS MODIFICATION BY DRUGS</title><title>British journal of pharmacology</title><addtitle>Br J Pharmacol</addtitle><description>1Gas chromatographic and radio‐isotope labelling techniques have been used to establish the origin of the arachidonic acid used by the platelet cyclo‐oxygenase for the synthesis of pro‐aggregatory prostaglandin endoperoxide derivatives.
2Measurements of total platelet arachidonate content indicated that more than 95% is esterified in the phosphatide fraction of the cells.
3During aggregation by collagen or thrombin as much as 80% of the total platelet arachidonate may be liberated and transformed by the platelet enzymes into hydroxyacids and other more polar compounds.
4The phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol fractions are major sources of the arachidonate thus used.
5Indomethacin, which prevents platelet aggregation by inhibiting the cyclo‐oxygenase, did not affect this release of arachidonate from the phosphatides but did prevent the transformation of arachidonate to endoperoxide derivatives.
6Mepacrine, a drug which possesses weak anti‐phospholipase activity in platelets, also prevents aggregation by collagen or thrombin, but seems to do so by preventing substrate release from the phosphatide fraction.
7It is suggested that phospholipase A2 plays a key role in the initial events during platelet aggregation induced by collagen.</description><subject>Animals</subject><subject>Arachidonic Acids - blood</subject><subject>Blood Platelets - analysis</subject><subject>Blood Platelets - drug effects</subject><subject>Blood Platelets - enzymology</subject><subject>Blood Platelets - metabolism</subject><subject>Carbon Radioisotopes</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Chromatography, Gas</subject><subject>Collagen - pharmacology</subject><subject>Fatty Acids - blood</subject><subject>Hydrolysis</subject><subject>In Vitro Techniques</subject><subject>Indomethacin - pharmacology</subject><subject>Lipids - blood</subject><subject>Male</subject><subject>Phosphatidylcholines - metabolism</subject><subject>Phospholipases - blood</subject><subject>Phospholipids - blood</subject><subject>Phosphorus - blood</subject><subject>Platelet Aggregation - drug effects</subject><subject>Quinacrine - pharmacology</subject><subject>Rabbits</subject><subject>Thrombin - pharmacology</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkUuPmzAUha2qr8y0_6ALq4vuYGwcbOiilXmEWCIwImTRleU4pCUiYQaSmcy_HyOiqF3WG_v6O_f4ygeArxjZ2Ky7nY2njFou8bCNfcbs4xoxFyH7_AZMrugtmCCEmIWx530EN32_Q8hA5n4A7z3CkEMm4FzOYxiJZVmIYFWKPIM8i-AiLnmQp2K5gPkM8oKHcxHlmQghD0UERQYLHgSihPcpL-M0LpcwWhUiSyBPkiJO-NVJGLTIIzET4XgZ_IJRsUqWn8C7rWr66vNlvwWrWVyGcyvNE6NNLT11GbKoRtrDDDsEEeor5Kgp2VB3rajeIKJNofAW-752nQ310BZrHzueyxwHaaI9Sm7Bj9H34bTeVxtdHY6dauRDV-9V9yJbVct_yaH-I3-3TxJTyhjxjcG3i0HXPp6q_ij3da-rplGHqj310vwkIWZWI_w-CnXX9n1Xba-PYCSH2ORODtnIIRs5xCYvscmzaf7y95jX1jEng3-O-Lluqpf_MJbB_Xw4kVfvxp3J</recordid><startdate>197702</startdate><enddate>197702</enddate><creator>BLACKWELL, G.J.</creator><creator>DUNCOMBE, W.G.</creator><creator>FLOWER, R.J.</creator><creator>PARSONS, M.F.</creator><creator>VANE, J.R.</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>197702</creationdate><title>THE DISTRIBUTION AND METABOLISM OF ARACHIDONIC ACID IN RABBIT PLATELETS DURING AGGREGATION AND ITS MODIFICATION BY DRUGS</title><author>BLACKWELL, G.J. ; DUNCOMBE, W.G. ; FLOWER, R.J. ; PARSONS, M.F. ; VANE, J.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4570-6c0c8171230369a02a43d65ba6cd03c43da1f199c52d680f1c912857220c3c863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Animals</topic><topic>Arachidonic Acids - blood</topic><topic>Blood Platelets - analysis</topic><topic>Blood Platelets - drug effects</topic><topic>Blood Platelets - enzymology</topic><topic>Blood Platelets - metabolism</topic><topic>Carbon Radioisotopes</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Chromatography, Gas</topic><topic>Collagen - pharmacology</topic><topic>Fatty Acids - blood</topic><topic>Hydrolysis</topic><topic>In Vitro Techniques</topic><topic>Indomethacin - pharmacology</topic><topic>Lipids - blood</topic><topic>Male</topic><topic>Phosphatidylcholines - metabolism</topic><topic>Phospholipases - blood</topic><topic>Phospholipids - blood</topic><topic>Phosphorus - blood</topic><topic>Platelet Aggregation - drug effects</topic><topic>Quinacrine - pharmacology</topic><topic>Rabbits</topic><topic>Thrombin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BLACKWELL, G.J.</creatorcontrib><creatorcontrib>DUNCOMBE, W.G.</creatorcontrib><creatorcontrib>FLOWER, R.J.</creatorcontrib><creatorcontrib>PARSONS, M.F.</creatorcontrib><creatorcontrib>VANE, J.R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BLACKWELL, G.J.</au><au>DUNCOMBE, W.G.</au><au>FLOWER, R.J.</au><au>PARSONS, M.F.</au><au>VANE, J.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>THE DISTRIBUTION AND METABOLISM OF ARACHIDONIC ACID IN RABBIT PLATELETS DURING AGGREGATION AND ITS MODIFICATION BY DRUGS</atitle><jtitle>British journal of pharmacology</jtitle><addtitle>Br J Pharmacol</addtitle><date>1977-02</date><risdate>1977</risdate><volume>59</volume><issue>2</issue><spage>353</spage><epage>366</epage><pages>353-366</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><abstract>1Gas chromatographic and radio‐isotope labelling techniques have been used to establish the origin of the arachidonic acid used by the platelet cyclo‐oxygenase for the synthesis of pro‐aggregatory prostaglandin endoperoxide derivatives.
2Measurements of total platelet arachidonate content indicated that more than 95% is esterified in the phosphatide fraction of the cells.
3During aggregation by collagen or thrombin as much as 80% of the total platelet arachidonate may be liberated and transformed by the platelet enzymes into hydroxyacids and other more polar compounds.
4The phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol fractions are major sources of the arachidonate thus used.
5Indomethacin, which prevents platelet aggregation by inhibiting the cyclo‐oxygenase, did not affect this release of arachidonate from the phosphatides but did prevent the transformation of arachidonate to endoperoxide derivatives.
6Mepacrine, a drug which possesses weak anti‐phospholipase activity in platelets, also prevents aggregation by collagen or thrombin, but seems to do so by preventing substrate release from the phosphatide fraction.
7It is suggested that phospholipase A2 plays a key role in the initial events during platelet aggregation induced by collagen.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>837023</pmid><doi>10.1111/j.1476-5381.1977.tb07500.x</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Arachidonic Acids - blood Blood Platelets - analysis Blood Platelets - drug effects Blood Platelets - enzymology Blood Platelets - metabolism Carbon Radioisotopes Chemical Phenomena Chemistry Chromatography, Gas Collagen - pharmacology Fatty Acids - blood Hydrolysis In Vitro Techniques Indomethacin - pharmacology Lipids - blood Male Phosphatidylcholines - metabolism Phospholipases - blood Phospholipids - blood Phosphorus - blood Platelet Aggregation - drug effects Quinacrine - pharmacology Rabbits Thrombin - pharmacology |
title | THE DISTRIBUTION AND METABOLISM OF ARACHIDONIC ACID IN RABBIT PLATELETS DURING AGGREGATION AND ITS MODIFICATION BY DRUGS |
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