novel endonuclease IV post-PCR genotyping system

Here we describe a novel endonuclease IV (Endo IV) based assay utilizing a substrate that mimics the abasic lesions that normally occur in double-stranded DNA. The three component substrate is characterized by single-stranded DNA target, an oligonucleotide probe, separated from a helper oligonucleot...

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Veröffentlicht in:	Nucleic acids research 2006-11, Vol.34 (19), p.e128-e128
Hauptverfasser:	Kutyavin, Igor V, Milesi, Dave, Belousov, Yevgeniy, Podyminogin, Mikhail, Vorobiev, Alexei, Gorn, Vladimir, Lukhtanov, Eugeny A, Vermeulen, Nicolaas M.J, Mahoney, Walt
Format:	Artikel
Sprache:	eng
Schlagworte:	Agouti Signaling Protein Alleles Base Pair Mismatch Deoxyribonuclease IV (Phage T4-Induced) - metabolism DNA Breaks, Double-Stranded Escherichia coli Proteins - metabolism Fluorescent Dyes - chemistry Genes, APC Genotype Intercellular Signaling Peptides and Proteins - genetics Methods Online Nucleic Acid Hybridization - methods Oligonucleotide Probes - chemical synthesis Polymerase Chain Reaction Polymorphism, Single Nucleotide Thermodynamics
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