Species-specific SCAR markers for authentication of Sinocalycanthus chinensis
Sinocalycanthus chinensis, an endangered species endemic to China, is cultivated as an ornamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In t...
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| Veröffentlicht in: | Journal of Zhejiang University. B. Science 2006-11, Vol.7 (11), p.868-872 |
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| creator | Ye, Qian Qiu, Ying-xiong Quo, Yan-qi Chen, Jian-xin Yang, Shu-zhen Zhao, Ming-shui Fu, Cheng-xin |
| description | Sinocalycanthus chinensis, an endangered species endemic to China, is cultivated as an ornamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs of S. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chinensis in seedling market. |
| doi_str_mv | 10.1631/jzus.2006.b0868 |
| format | Article |
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However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs of S. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chinensis in seedling market.</description><identifier>ISSN: 1673-1581</identifier><identifier>EISSN: 1862-1783</identifier><identifier>DOI: 10.1631/jzus.2006.b0868</identifier><identifier>PMID: 17048299</identifier><language>eng</language><publisher>China: Springer Nature B.V</publisher><subject>Biotechnology ; Calycanthaceae - genetics ; Calycanthus floridus ; DNA, Plant - genetics ; Genetic Markers - genetics ; Genetics ; Plant Leaves - genetics ; Random Amplified Polymorphic DNA Technique ; Species Specificity</subject><ispartof>Journal of Zhejiang University. B. Science, 2006-11, Vol.7 (11), p.868-872</ispartof><rights>Zhejiang University 2006</rights><rights>Copyright © Wanfang Data Co. Ltd. All Rights Reserved.</rights><rights>Copyright © 2006, Journal of Zhejiang University Science 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4653-174a25682f3e168908298ab51a66e3d65495aae91fc5e126f0ebdec1a07574603</citedby><cites>FETCH-LOGICAL-c4653-174a25682f3e168908298ab51a66e3d65495aae91fc5e126f0ebdec1a07574603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.wanfangdata.com.cn/images/PeriodicalImages/zjdxxbb-e/zjdxxbb-e.jpg</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635811/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635811/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17048299$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ye, Qian</creatorcontrib><creatorcontrib>Qiu, Ying-xiong</creatorcontrib><creatorcontrib>Quo, Yan-qi</creatorcontrib><creatorcontrib>Chen, Jian-xin</creatorcontrib><creatorcontrib>Yang, Shu-zhen</creatorcontrib><creatorcontrib>Zhao, Ming-shui</creatorcontrib><creatorcontrib>Fu, Cheng-xin</creatorcontrib><title>Species-specific SCAR markers for authentication of Sinocalycanthus chinensis</title><title>Journal of Zhejiang University. B. Science</title><addtitle>J Zhejiang Univ Sci B</addtitle><description>Sinocalycanthus chinensis, an endangered species endemic to China, is cultivated as an ornamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs of S. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chinensis in seedling market.</description><subject>Biotechnology</subject><subject>Calycanthaceae - genetics</subject><subject>Calycanthus floridus</subject><subject>DNA, Plant - genetics</subject><subject>Genetic Markers - genetics</subject><subject>Genetics</subject><subject>Plant Leaves - genetics</subject><subject>Random Amplified Polymorphic DNA Technique</subject><subject>Species Specificity</subject><issn>1673-1581</issn><issn>1862-1783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFUU1v1DAQtSoQLYVzb1XUAwekbD1xYjuXSmXFl9SqEgtny_GOu16y9mIn0PbX42hXLXDhNJb85s37IOQE6Aw4g_P1w5hmFaV81lHJ5QE5AsmrEoRkz_KbC1ZCI-GQvExpTWldU8FfkEMQtJZV2x6R68UWjcNUpmlaZ4rF_PJLsdHxO8ZU2BALPQ4r9IMzenDBF8EWC-eD0f290X5YjakwK-fRJ5dekedW9wlf7-cx-fbh_df5p_Lq5uPn-eVVaWreZEmi1lXDZWUZApctzVqk7hrQnCNb8qZuG62xBWsahIpbit0SDWgqGlFzyo7JxY53O3YbXJosL-pebaPLwu9V0E79_ePdSt2GnyqHluOATPB2R_BLe6v9rVqHMfosWT2sl3d3XadwShWAUpbBb_bXYvgxYhrUxiWDfa89hjGp7IBzTsV_gdAKAMFkBp79A3y8LyVjUtTVxHa-A5kYUopoH_0BnYyAmtpXk071bmo_b5z-GcsTfl83-w3A2qva</recordid><startdate>200611</startdate><enddate>200611</enddate><creator>Ye, Qian</creator><creator>Qiu, Ying-xiong</creator><creator>Quo, Yan-qi</creator><creator>Chen, Jian-xin</creator><creator>Yang, Shu-zhen</creator><creator>Zhao, Ming-shui</creator><creator>Fu, Cheng-xin</creator><general>Springer Nature B.V</general><general>Laboratory of Systematic and Evolutionary Botany and Biodiversity, School of Life Sciences, Zhejiang University, Hangzhou 310029, China%Management Bureau of Tianmushan National Nature Conservative Area, Lin'an 311311, China</general><general>Zhejiang University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7QP</scope><scope>7TK</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>M7S</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PCBAR</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>7ST</scope><scope>7U6</scope><scope>C1K</scope><scope>7X8</scope><scope>2B.</scope><scope>4A8</scope><scope>92I</scope><scope>93N</scope><scope>PSX</scope><scope>TCJ</scope><scope>5PM</scope></search><sort><creationdate>200611</creationdate><title>Species-specific SCAR markers for authentication of Sinocalycanthus chinensis</title><author>Ye, Qian ; Qiu, Ying-xiong ; Quo, Yan-qi ; Chen, Jian-xin ; Yang, Shu-zhen ; Zhao, Ming-shui ; Fu, Cheng-xin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4653-174a25682f3e168908298ab51a66e3d65495aae91fc5e126f0ebdec1a07574603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Biotechnology</topic><topic>Calycanthaceae - 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B. Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ye, Qian</au><au>Qiu, Ying-xiong</au><au>Quo, Yan-qi</au><au>Chen, Jian-xin</au><au>Yang, Shu-zhen</au><au>Zhao, Ming-shui</au><au>Fu, Cheng-xin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Species-specific SCAR markers for authentication of Sinocalycanthus chinensis</atitle><jtitle>Journal of Zhejiang University. B. Science</jtitle><addtitle>J Zhejiang Univ Sci B</addtitle><date>2006-11</date><risdate>2006</risdate><volume>7</volume><issue>11</issue><spage>868</spage><epage>872</epage><pages>868-872</pages><issn>1673-1581</issn><eissn>1862-1783</eissn><abstract>Sinocalycanthus chinensis, an endangered species endemic to China, is cultivated as an ornamental landscape tree in China. However, S. chinensis, Chimonanthus species and Calycanthus floridus are difficult to be distinguished in seedling market because of their similar morphological characters. In this study, ISSR (inter-simple sequence repeats) were applied to detect S. chinensis from its closely related species. A unique 748-bp band was found in all accessions of S. chinensis. SCAR (sequence characterized amplified regions) markers were created by cloning and sequencing the specific band, and designing a pair of primers to amplify the band of 748 bp. Diagnostic PCRs were performed using the primer pair with the total DNAs of S. chinensis, Chimonanthus species and C. floridus as templates, with only S. chinensis being able to be amplified. This amplification is not only rapid (results can be obtained in less than 3 h), but is also easy to perform. Hence it is a feasible method for identifying S. chinensis in seedling market.</abstract><cop>China</cop><pub>Springer Nature B.V</pub><pmid>17048299</pmid><doi>10.1631/jzus.2006.b0868</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Biotechnology Calycanthaceae - genetics Calycanthus floridus DNA, Plant - genetics Genetic Markers - genetics Genetics Plant Leaves - genetics Random Amplified Polymorphic DNA Technique Species Specificity |
| title | Species-specific SCAR markers for authentication of Sinocalycanthus chinensis |
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