Carotenoid Cleavage Dioxygenase (CmCCD4a) Contributes to White Color Formation in Chrysanthemum Petals
The white petals of chrysanthemum (Chrysanthemum morifolium Ramat.) are believed to contain a factor that inhibits the accumulation of carotenoids. To find this factor, we performed polymerase chain reaction-Select subtraction screening and obtained a clone expressed differentially in white and yell...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2006-11, Vol.142 (3), p.1193-1201 |
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| creator | Ohmiya, Akemi Kishimoto, Sanae Aida, Ryutaro Yoshioka, Satoshi Sumitomo, Katsuhiko |
| description | The white petals of chrysanthemum (Chrysanthemum morifolium Ramat.) are believed to contain a factor that inhibits the accumulation of carotenoids. To find this factor, we performed polymerase chain reaction-Select subtraction screening and obtained a clone expressed differentially in white and yellow petals. The deduced amino acid sequence of the protein (designated CmCCD4a) encoded by the clone was highly homologous to the sequence of carotenoid cleavage dioxygenase. All the white-flowered chrysanthemum cultivars tested showed high levels of CmCCD4a transcript in their petals, whereas most of the yellow-flowered cultivars showed extremely low levels. Expression of CmCCD4a was strictly limited to flower petals and was not detected in other organs, such as the root, stem, or leaf. White petals turned yellow after the RNAi construct of CmCCD4a was introduced. These results indicate that in white petals of chrysanthemums, carotenoids are synthesized but are subsequently degraded into colorless compounds, which results in the white color. |
| doi_str_mv | 10.1104/pp.106.087130 |
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To find this factor, we performed polymerase chain reaction-Select subtraction screening and obtained a clone expressed differentially in white and yellow petals. The deduced amino acid sequence of the protein (designated CmCCD4a) encoded by the clone was highly homologous to the sequence of carotenoid cleavage dioxygenase. All the white-flowered chrysanthemum cultivars tested showed high levels of CmCCD4a transcript in their petals, whereas most of the yellow-flowered cultivars showed extremely low levels. Expression of CmCCD4a was strictly limited to flower petals and was not detected in other organs, such as the root, stem, or leaf. White petals turned yellow after the RNAi construct of CmCCD4a was introduced. These results indicate that in white petals of chrysanthemums, carotenoids are synthesized but are subsequently degraded into colorless compounds, which results in the white color.</description><identifier>ISSN: 0032-0889</identifier><identifier>ISSN: 1532-2548</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.106.087130</identifier><identifier>PMID: 16980560</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>Amino Acid Sequence ; Amino acids ; Biological and medical sciences ; Carotenoids ; Chrysanthemum - enzymology ; Chrysanthemum - genetics ; Color ; Complementary DNA ; Dioxygenases - genetics ; Dioxygenases - metabolism ; Flowers ; Flowers - genetics ; Flowers - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant - physiology ; Genomics ; Metabolism ; Molecular Sequence Data ; Petals ; Phylogeny ; Plant morphology ; Plant physiology and development ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants ; Polymerase chain reaction ; Reverse transcriptase polymerase chain reaction ; RNA Interference ; Storage and secretion, pigments, phytochrome ; Systems Biology, Molecular Biology, and Gene Regulation</subject><ispartof>Plant physiology (Bethesda), 2006-11, Vol.142 (3), p.1193-1201</ispartof><rights>Copyright 2006 American Society of Plant Biologists</rights><rights>2007 INIST-CNRS</rights><rights>Copyright © 2006, American Society of Plant Biologists 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c608t-6b7c2b47d0d99ee17dbca0d1698d7cc2a78f2060f60c1509dc64ea3994c954963</citedby><cites>FETCH-LOGICAL-c608t-6b7c2b47d0d99ee17dbca0d1698d7cc2a78f2060f60c1509dc64ea3994c954963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/20206007$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/20206007$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18274830$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16980560$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohmiya, Akemi</creatorcontrib><creatorcontrib>Kishimoto, Sanae</creatorcontrib><creatorcontrib>Aida, Ryutaro</creatorcontrib><creatorcontrib>Yoshioka, Satoshi</creatorcontrib><creatorcontrib>Sumitomo, Katsuhiko</creatorcontrib><title>Carotenoid Cleavage Dioxygenase (CmCCD4a) Contributes to White Color Formation in Chrysanthemum Petals</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The white petals of chrysanthemum (Chrysanthemum morifolium Ramat.) are believed to contain a factor that inhibits the accumulation of carotenoids. To find this factor, we performed polymerase chain reaction-Select subtraction screening and obtained a clone expressed differentially in white and yellow petals. The deduced amino acid sequence of the protein (designated CmCCD4a) encoded by the clone was highly homologous to the sequence of carotenoid cleavage dioxygenase. All the white-flowered chrysanthemum cultivars tested showed high levels of CmCCD4a transcript in their petals, whereas most of the yellow-flowered cultivars showed extremely low levels. Expression of CmCCD4a was strictly limited to flower petals and was not detected in other organs, such as the root, stem, or leaf. White petals turned yellow after the RNAi construct of CmCCD4a was introduced. These results indicate that in white petals of chrysanthemums, carotenoids are synthesized but are subsequently degraded into colorless compounds, which results in the white color.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Biological and medical sciences</subject><subject>Carotenoids</subject><subject>Chrysanthemum - enzymology</subject><subject>Chrysanthemum - genetics</subject><subject>Color</subject><subject>Complementary DNA</subject><subject>Dioxygenases - genetics</subject><subject>Dioxygenases - metabolism</subject><subject>Flowers</subject><subject>Flowers - genetics</subject><subject>Flowers - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Genomics</subject><subject>Metabolism</subject><subject>Molecular Sequence Data</subject><subject>Petals</subject><subject>Phylogeny</subject><subject>Plant morphology</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants</subject><subject>Polymerase chain reaction</subject><subject>Reverse transcriptase polymerase chain reaction</subject><subject>RNA Interference</subject><subject>Storage and secretion, pigments, phytochrome</subject><subject>Systems Biology, Molecular Biology, and Gene Regulation</subject><issn>0032-0889</issn><issn>1532-2548</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1v1DAQxS0EokvhyBHwhQoOWcaJ448LEkopIFUCCSqOltdxdl0lcWo7Ffvf421WLZxm5PfT89M8hF4SWBMC9MM0rQmwNQhOKniEVqSuyqKsqXiMVgB5ByHkCXoW4zUAkIrQp-iEMCmgZrBCXaODT3b0rsVNb_Wt3lp87vyf_daOOlr8rhma5pzq97jxYwpuMycbcfL4984lmx97H_CFD4NOzo_YjbjZhX3UY9rZYR7wD5t0H5-jJ10e9sVxnqKri8-_mq_F5fcv35pPl4VhIFLBNtyUG8pbaKW0lvB2YzS0h7gtN6bUXHQlMOgYGFKDbA2jVldSUiNrKll1ij4uvtO8GWxrbI6sezUFN-iwV1479b8yup3a-ltFWAW8ltng7GgQ_M1sY1KDi8b2vR6tn6NigpSccZ7BYgFN8DEG291_QkAdmlHTlFemlmYy__rfZA_0sYoMvD0COhrdd0GPxsUHTpScijujVwt3HZMP93oJh8PAIdibRe-0V3obssfVzzI3D4QQSkpa_QV8kqo6</recordid><startdate>20061101</startdate><enddate>20061101</enddate><creator>Ohmiya, Akemi</creator><creator>Kishimoto, Sanae</creator><creator>Aida, Ryutaro</creator><creator>Yoshioka, Satoshi</creator><creator>Sumitomo, Katsuhiko</creator><general>American Society of Plant Biologists</general><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20061101</creationdate><title>Carotenoid Cleavage Dioxygenase (CmCCD4a) Contributes to White Color Formation in Chrysanthemum Petals</title><author>Ohmiya, Akemi ; Kishimoto, Sanae ; Aida, Ryutaro ; Yoshioka, Satoshi ; Sumitomo, Katsuhiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c608t-6b7c2b47d0d99ee17dbca0d1698d7cc2a78f2060f60c1509dc64ea3994c954963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Biological and medical sciences</topic><topic>Carotenoids</topic><topic>Chrysanthemum - enzymology</topic><topic>Chrysanthemum - genetics</topic><topic>Color</topic><topic>Complementary DNA</topic><topic>Dioxygenases - genetics</topic><topic>Dioxygenases - metabolism</topic><topic>Flowers</topic><topic>Flowers - genetics</topic><topic>Flowers - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>Genomics</topic><topic>Metabolism</topic><topic>Molecular Sequence Data</topic><topic>Petals</topic><topic>Phylogeny</topic><topic>Plant morphology</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plants</topic><topic>Polymerase chain reaction</topic><topic>Reverse transcriptase polymerase chain reaction</topic><topic>RNA Interference</topic><topic>Storage and secretion, pigments, phytochrome</topic><topic>Systems Biology, Molecular Biology, and Gene Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohmiya, Akemi</creatorcontrib><creatorcontrib>Kishimoto, Sanae</creatorcontrib><creatorcontrib>Aida, Ryutaro</creatorcontrib><creatorcontrib>Yoshioka, Satoshi</creatorcontrib><creatorcontrib>Sumitomo, Katsuhiko</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohmiya, Akemi</au><au>Kishimoto, Sanae</au><au>Aida, Ryutaro</au><au>Yoshioka, Satoshi</au><au>Sumitomo, Katsuhiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carotenoid Cleavage Dioxygenase (CmCCD4a) Contributes to White Color Formation in Chrysanthemum Petals</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2006-11-01</date><risdate>2006</risdate><volume>142</volume><issue>3</issue><spage>1193</spage><epage>1201</epage><pages>1193-1201</pages><issn>0032-0889</issn><issn>1532-2548</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The white petals of chrysanthemum (Chrysanthemum morifolium Ramat.) are believed to contain a factor that inhibits the accumulation of carotenoids. To find this factor, we performed polymerase chain reaction-Select subtraction screening and obtained a clone expressed differentially in white and yellow petals. The deduced amino acid sequence of the protein (designated CmCCD4a) encoded by the clone was highly homologous to the sequence of carotenoid cleavage dioxygenase. All the white-flowered chrysanthemum cultivars tested showed high levels of CmCCD4a transcript in their petals, whereas most of the yellow-flowered cultivars showed extremely low levels. Expression of CmCCD4a was strictly limited to flower petals and was not detected in other organs, such as the root, stem, or leaf. White petals turned yellow after the RNAi construct of CmCCD4a was introduced. These results indicate that in white petals of chrysanthemums, carotenoids are synthesized but are subsequently degraded into colorless compounds, which results in the white color.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Biologists</pub><pmid>16980560</pmid><doi>10.1104/pp.106.087130</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Amino acids Biological and medical sciences Carotenoids Chrysanthemum - enzymology Chrysanthemum - genetics Color Complementary DNA Dioxygenases - genetics Dioxygenases - metabolism Flowers Flowers - genetics Flowers - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant - physiology Genomics Metabolism Molecular Sequence Data Petals Phylogeny Plant morphology Plant physiology and development Plant Proteins - genetics Plant Proteins - metabolism Plants Polymerase chain reaction Reverse transcriptase polymerase chain reaction RNA Interference Storage and secretion, pigments, phytochrome Systems Biology, Molecular Biology, and Gene Regulation |
| title | Carotenoid Cleavage Dioxygenase (CmCCD4a) Contributes to White Color Formation in Chrysanthemum Petals |
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