Nucleotide sequence of a Brassica napus endochitinase gene
Endochitinase (EC 3.2.1.14, chitinase), which catalyzes the hydrolysis of the beta -1,4-linkages of the N-acetyl-D-glucosamine of the fungal cell wall polymer chitin, is involved in inducible defenses of plants against potential pathogens. Here we report the nucleotide and deduced amino acid sequenc...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1993-04, Vol.101 (4), p.1403-1403 |
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| creator | Hamel, F Bellemare, G |
| description | Endochitinase (EC 3.2.1.14, chitinase), which catalyzes the hydrolysis of the beta -1,4-linkages of the N-acetyl-D-glucosamine of the fungal cell wall polymer chitin, is involved in inducible defenses of plants against potential pathogens. Here we report the nucleotide and deduced amino acid sequences of a genomic clone (BnCh25) encoding a class I endochitinase from Brassica napus cv Westar. The nucleotide sequence of the coding region of this B. napus chitinase is 86.5% homologous with an endochitinase from Arabidopsis thaliana, 70.0% with one from Nicotiana tabacum, and 69.1% with one from Phaseolus vulgaris. Furthermore, the B. napus gene presents the same expression pattern as an organ-specific basic chitinase gene from A. thaliana that was shown to be expressed at high constitutive level in roots, with lower levels in leaves and flowering shoots from healthy plants. |
| doi_str_mv | 10.1104/pp.101.4.1403 |
| format | Article |
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Here we report the nucleotide and deduced amino acid sequences of a genomic clone (BnCh25) encoding a class I endochitinase from Brassica napus cv Westar. The nucleotide sequence of the coding region of this B. napus chitinase is 86.5% homologous with an endochitinase from Arabidopsis thaliana, 70.0% with one from Nicotiana tabacum, and 69.1% with one from Phaseolus vulgaris. Furthermore, the B. napus gene presents the same expression pattern as an organ-specific basic chitinase gene from A. thaliana that was shown to be expressed at high constitutive level in roots, with lower levels in leaves and flowering shoots from healthy plants.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.101.4.1403</identifier><identifier>PMID: 8310072</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>AMINO ACID SEQUENCES ; Base Sequence ; Biological and medical sciences ; Brassica - enzymology ; Brassica - genetics ; BRASSICA NAPUS ; Cell walls ; Chitin ; CHITINASE ; Chitinases - genetics ; DNA ; EXONS ; Fundamental and applied biological sciences. Psychology ; genbank/m95835 ; GENE ; GENES ; Genes. Genome ; INTRONS ; Leaves ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; NUCLEOTIDE SEQUENCE ; Nucleotide sequences ; Plant Gene Register ; Plants ; Proteins ; QUITINASA ; SECUENCIA NUCLEICA ; SEQUENCE NUCLEIQUE ; STRUCTURAL GENES</subject><ispartof>Plant physiology (Bethesda), 1993-04, Vol.101 (4), p.1403-1403</ispartof><rights>Copyright 1993 American Society of Plant Physiologists</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-5e70989312417479dd53d3125eefa0ef181b920310a8f481d26373e6eb40010f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4275129$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4275129$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4828921$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8310072$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hamel, F</creatorcontrib><creatorcontrib>Bellemare, G</creatorcontrib><title>Nucleotide sequence of a Brassica napus endochitinase gene</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Endochitinase (EC 3.2.1.14, chitinase), which catalyzes the hydrolysis of the beta -1,4-linkages of the N-acetyl-D-glucosamine of the fungal cell wall polymer chitin, is involved in inducible defenses of plants against potential pathogens. Here we report the nucleotide and deduced amino acid sequences of a genomic clone (BnCh25) encoding a class I endochitinase from Brassica napus cv Westar. The nucleotide sequence of the coding region of this B. napus chitinase is 86.5% homologous with an endochitinase from Arabidopsis thaliana, 70.0% with one from Nicotiana tabacum, and 69.1% with one from Phaseolus vulgaris. Furthermore, the B. napus gene presents the same expression pattern as an organ-specific basic chitinase gene from A. thaliana that was shown to be expressed at high constitutive level in roots, with lower levels in leaves and flowering shoots from healthy plants.</description><subject>AMINO ACID SEQUENCES</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Brassica - enzymology</subject><subject>Brassica - genetics</subject><subject>BRASSICA NAPUS</subject><subject>Cell walls</subject><subject>Chitin</subject><subject>CHITINASE</subject><subject>Chitinases - genetics</subject><subject>DNA</subject><subject>EXONS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genbank/m95835</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes. Genome</subject><subject>INTRONS</subject><subject>Leaves</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>Nucleotide sequences</subject><subject>Plant Gene Register</subject><subject>Plants</subject><subject>Proteins</subject><subject>QUITINASA</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>STRUCTURAL GENES</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhS0EKkvhyAWBlAPilmXGdhIHiQNUUJAqOEDPltcZb11l7WAnlfj3ddnVFk6cPNb73tMbDWPPEdaIIN9O0xoB13KNEsQDtsJG8Jo3Uj1kK4Ayg1L9Y_Yk52sAQIHyhJ0ogQAdX7F33xY7Upz9QFWmXwsFS1V0lak-JpOzt6YKZlpyRWGI9srPPphM1ZYCPWWPnBkzPTu8p-zy86efZ1_qi-_nX88-XNRWKpzrhjroVS-QS-xk1w9DI4bya4icAXKocNNzKIWMcsUx8FZ0glrayFIXnDhl7_e507LZ0WApzMmMekp-Z9JvHY3X_yrBX-ltvNHYQttB8b85-FMsC-ZZ73y2NI4mUFyy7lpeOnTivyC2qizwJ7HegzbFnBO5YxkEfXcUPU1lRC313VEK_-rvDY704QpFf33QTbZmdMkE6_MRk4qrnmPBXu6x6zzHdC_zrkHeF_nFXnYmarNNJeHyRy-xb0UrbgGyKKTe</recordid><startdate>19930401</startdate><enddate>19930401</enddate><creator>Hamel, F</creator><creator>Bellemare, G</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930401</creationdate><title>Nucleotide sequence of a Brassica napus endochitinase gene</title><author>Hamel, F ; Bellemare, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-5e70989312417479dd53d3125eefa0ef181b920310a8f481d26373e6eb40010f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>AMINO ACID SEQUENCES</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Brassica - enzymology</topic><topic>Brassica - genetics</topic><topic>BRASSICA NAPUS</topic><topic>Cell walls</topic><topic>Chitin</topic><topic>CHITINASE</topic><topic>Chitinases - genetics</topic><topic>DNA</topic><topic>EXONS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genbank/m95835</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes. Genome</topic><topic>INTRONS</topic><topic>Leaves</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>Nucleotide sequences</topic><topic>Plant Gene Register</topic><topic>Plants</topic><topic>Proteins</topic><topic>QUITINASA</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>STRUCTURAL GENES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hamel, F</creatorcontrib><creatorcontrib>Bellemare, G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamel, F</au><au>Bellemare, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nucleotide sequence of a Brassica napus endochitinase gene</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1993-04-01</date><risdate>1993</risdate><volume>101</volume><issue>4</issue><spage>1403</spage><epage>1403</epage><pages>1403-1403</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Endochitinase (EC 3.2.1.14, chitinase), which catalyzes the hydrolysis of the beta -1,4-linkages of the N-acetyl-D-glucosamine of the fungal cell wall polymer chitin, is involved in inducible defenses of plants against potential pathogens. Here we report the nucleotide and deduced amino acid sequences of a genomic clone (BnCh25) encoding a class I endochitinase from Brassica napus cv Westar. The nucleotide sequence of the coding region of this B. napus chitinase is 86.5% homologous with an endochitinase from Arabidopsis thaliana, 70.0% with one from Nicotiana tabacum, and 69.1% with one from Phaseolus vulgaris. Furthermore, the B. napus gene presents the same expression pattern as an organ-specific basic chitinase gene from A. thaliana that was shown to be expressed at high constitutive level in roots, with lower levels in leaves and flowering shoots from healthy plants.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>8310072</pmid><doi>10.1104/pp.101.4.1403</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | AMINO ACID SEQUENCES Base Sequence Biological and medical sciences Brassica - enzymology Brassica - genetics BRASSICA NAPUS Cell walls Chitin CHITINASE Chitinases - genetics DNA EXONS Fundamental and applied biological sciences. Psychology genbank/m95835 GENE GENES Genes. Genome INTRONS Leaves Molecular and cellular biology Molecular genetics Molecular Sequence Data NUCLEOTIDE SEQUENCE Nucleotide sequences Plant Gene Register Plants Proteins QUITINASA SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE STRUCTURAL GENES |
| title | Nucleotide sequence of a Brassica napus endochitinase gene |
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