DNA Damage Levels Determine Cyclobutyl Pyrimidine Dimer Repair Mechanisms in Alfalfa Seedlings
Ultraviolet radiation in sunlight damages DNA in plants, but little is understood about the types, lesion capacity, and coordination of repair pathways. We challenged intact alfalfa seedlings with UV doses that induced different initial levels of cyclobutyl pyrimidine dimers and measured repair by e...
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Veröffentlicht in: | The Plant cell 1994-11, Vol.6 (11), p.1635-1641 |
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creator | Quaite, F. Elsie Takayanagi, Shinnosuke Ruffini, Janice Sutherland, C. Sutherland, Betsy M. |
description | Ultraviolet radiation in sunlight damages DNA in plants, but little is understood about the types, lesion capacity, and coordination of repair pathways. We challenged intact alfalfa seedlings with UV doses that induced different initial levels of cyclobutyl pyrimidine dimers and measured repair by excision and photoreactivation. By using alkaline gel electrophoresis of nonradioactive DNAs treated with a cyclobutyl pyrimidine dimer-specific UV endonuclease, we quantitated ethidium-stained DNA by electronic imaging and calculated lesion frequencies from the number average molecular lengths. At low initial dimer frequencies (less than ∼30 dimers per million bases), the seedlings used only photoreactivation to repair dimers; excision repair was not significant. At higher damage levels, both excision and photorepair contributed significantly. This strategy would allow plants with low damage levels to use error-free repair requiring only an external light energy source, whereas seedlings subjected to higher damage frequencies could call on additional repair processes requiring cellular energy. Characterization of repair in plants thus requires an investigation of a range of conditions, including the level of initial damage. |
doi_str_mv | 10.1105/tpc.6.11.1635 |
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Elsie ; Takayanagi, Shinnosuke ; Ruffini, Janice ; Sutherland, C. ; Sutherland, Betsy M.</creator><creatorcontrib>Quaite, F. Elsie ; Takayanagi, Shinnosuke ; Ruffini, Janice ; Sutherland, C. ; Sutherland, Betsy M.</creatorcontrib><description>Ultraviolet radiation in sunlight damages DNA in plants, but little is understood about the types, lesion capacity, and coordination of repair pathways. We challenged intact alfalfa seedlings with UV doses that induced different initial levels of cyclobutyl pyrimidine dimers and measured repair by excision and photoreactivation. By using alkaline gel electrophoresis of nonradioactive DNAs treated with a cyclobutyl pyrimidine dimer-specific UV endonuclease, we quantitated ethidium-stained DNA by electronic imaging and calculated lesion frequencies from the number average molecular lengths. At low initial dimer frequencies (less than ∼30 dimers per million bases), the seedlings used only photoreactivation to repair dimers; excision repair was not significant. At higher damage levels, both excision and photorepair contributed significantly. This strategy would allow plants with low damage levels to use error-free repair requiring only an external light energy source, whereas seedlings subjected to higher damage frequencies could call on additional repair processes requiring cellular energy. 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Elsie</creatorcontrib><creatorcontrib>Takayanagi, Shinnosuke</creatorcontrib><creatorcontrib>Ruffini, Janice</creatorcontrib><creatorcontrib>Sutherland, C.</creatorcontrib><creatorcontrib>Sutherland, Betsy M.</creatorcontrib><title>DNA Damage Levels Determine Cyclobutyl Pyrimidine Dimer Repair Mechanisms in Alfalfa Seedlings</title><title>The Plant cell</title><addtitle>Plant Cell</addtitle><description>Ultraviolet radiation in sunlight damages DNA in plants, but little is understood about the types, lesion capacity, and coordination of repair pathways. We challenged intact alfalfa seedlings with UV doses that induced different initial levels of cyclobutyl pyrimidine dimers and measured repair by excision and photoreactivation. By using alkaline gel electrophoresis of nonradioactive DNAs treated with a cyclobutyl pyrimidine dimer-specific UV endonuclease, we quantitated ethidium-stained DNA by electronic imaging and calculated lesion frequencies from the number average molecular lengths. At low initial dimer frequencies (less than ∼30 dimers per million bases), the seedlings used only photoreactivation to repair dimers; excision repair was not significant. At higher damage levels, both excision and photorepair contributed significantly. This strategy would allow plants with low damage levels to use error-free repair requiring only an external light energy source, whereas seedlings subjected to higher damage frequencies could call on additional repair processes requiring cellular energy. Characterization of repair in plants thus requires an investigation of a range of conditions, including the level of initial damage.</description><subject>Alfalfa</subject><subject>Cephalopelvic disproportion</subject><subject>Dimers</subject><subject>DNA damage</subject><subject>Gels</subject><subject>Light</subject><subject>Plants</subject><subject>Pyrimidine dimers</subject><subject>Radiation damage</subject><subject>Seedlings</subject><issn>1040-4651</issn><issn>1532-298X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNpVkc1PGzEQxa0KVCj0yBX50AOXTf3t9aGHKKGAFKCirdRTLa93NhjtR7A3SPnv6ygRBcnSPI1_M_Okh9AZJRNKifw6rvxEZTmhissP6JhKzgpmyj8HWRNBCqEkPUKfUnoihFBNzUd0RBkTgrHyGP2d303x3HVuCXgBL9AmPIcRYhd6wLONb4dqPW5a_GMTQxfqbXceOoj4AVYuRHwL_tH1IXUJhx5P28blh38C1G3ol-kUHeZOgs_7eoJ-f7_8NbsuFvdXN7PpovBCqLHgTqtaVaxysm4kkdz4iijZEKK8AcNBSw5CO80aqqtSUSUoL50RnPhcPD9B33Z7V-uqg9pDP0bX2lU27eLGDi7Y9z99eLTL4cVSRaQwef5iPx-H5zWk0XYheWhb18OwTpaW0nAtS6IzWuxQH4eUIjSvVyix20hsjsSqLO02ksyfv7X2n95nkIEvO-ApjUN8u41xoi0vlTHZ4j9nopOx</recordid><startdate>19941101</startdate><enddate>19941101</enddate><creator>Quaite, F. Elsie</creator><creator>Takayanagi, Shinnosuke</creator><creator>Ruffini, Janice</creator><creator>Sutherland, C.</creator><creator>Sutherland, Betsy M.</creator><general>American Society of Plant Physiologists</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19941101</creationdate><title>DNA Damage Levels Determine Cyclobutyl Pyrimidine Dimer Repair Mechanisms in Alfalfa Seedlings</title><author>Quaite, F. Elsie ; Takayanagi, Shinnosuke ; Ruffini, Janice ; Sutherland, C. ; Sutherland, Betsy M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-3a76d6b2ba5df50539cb065f006c9e93e753e47a72f17b86164138a9430c8a9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Alfalfa</topic><topic>Cephalopelvic disproportion</topic><topic>Dimers</topic><topic>DNA damage</topic><topic>Gels</topic><topic>Light</topic><topic>Plants</topic><topic>Pyrimidine dimers</topic><topic>Radiation damage</topic><topic>Seedlings</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Quaite, F. Elsie</creatorcontrib><creatorcontrib>Takayanagi, Shinnosuke</creatorcontrib><creatorcontrib>Ruffini, Janice</creatorcontrib><creatorcontrib>Sutherland, C.</creatorcontrib><creatorcontrib>Sutherland, Betsy M.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Plant cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Quaite, F. Elsie</au><au>Takayanagi, Shinnosuke</au><au>Ruffini, Janice</au><au>Sutherland, C.</au><au>Sutherland, Betsy M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Damage Levels Determine Cyclobutyl Pyrimidine Dimer Repair Mechanisms in Alfalfa Seedlings</atitle><jtitle>The Plant cell</jtitle><addtitle>Plant Cell</addtitle><date>1994-11-01</date><risdate>1994</risdate><volume>6</volume><issue>11</issue><spage>1635</spage><epage>1641</epage><pages>1635-1641</pages><issn>1040-4651</issn><eissn>1532-298X</eissn><abstract>Ultraviolet radiation in sunlight damages DNA in plants, but little is understood about the types, lesion capacity, and coordination of repair pathways. We challenged intact alfalfa seedlings with UV doses that induced different initial levels of cyclobutyl pyrimidine dimers and measured repair by excision and photoreactivation. By using alkaline gel electrophoresis of nonradioactive DNAs treated with a cyclobutyl pyrimidine dimer-specific UV endonuclease, we quantitated ethidium-stained DNA by electronic imaging and calculated lesion frequencies from the number average molecular lengths. At low initial dimer frequencies (less than ∼30 dimers per million bases), the seedlings used only photoreactivation to repair dimers; excision repair was not significant. At higher damage levels, both excision and photorepair contributed significantly. This strategy would allow plants with low damage levels to use error-free repair requiring only an external light energy source, whereas seedlings subjected to higher damage frequencies could call on additional repair processes requiring cellular energy. Characterization of repair in plants thus requires an investigation of a range of conditions, including the level of initial damage.</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>12244228</pmid><doi>10.1105/tpc.6.11.1635</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Alma/SFX Local Collection |
subjects | Alfalfa Cephalopelvic disproportion Dimers DNA damage Gels Light Plants Pyrimidine dimers Radiation damage Seedlings |
title | DNA Damage Levels Determine Cyclobutyl Pyrimidine Dimer Repair Mechanisms in Alfalfa Seedlings |
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