S-RNase gene of Nicotiana alata is expressed in developing pollen
In the solanaceous plant Nicotiana alata, self-incompatibility is controlled by a single, multiallelic locus (S locus) expressed in both pollen and pistil. Previously, we have shown cosegregation between alleles of the S locus and alleles of a gene that encodes a glycoprotein with ribonuclease activ...
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| Veröffentlicht in: | The Plant cell 1993-12, Vol.5 (12), p.1771-1782 |
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| container_title | The Plant cell |
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| creator | Dodds, P.N Bonig, I Du, H Rodin, J Anderson, M.A Newbigin, E Clarke, A.E |
| description | In the solanaceous plant Nicotiana alata, self-incompatibility is controlled by a single, multiallelic locus (S locus) expressed in both pollen and pistil. Previously, we have shown cosegregation between alleles of the S locus and alleles of a gene that encodes a glycoprotein with ribonuclease activity (S-RNase). Furthermore, expression of the S-RNase gene is apparently confined to the pistil and is correlated with the onset of self-incompatibility. In this paper, we report that the S-RNase gene is also expressed at low levels in developing pollen. A transcript in developing pollen hybridized to a cDNA encoding the S2-RNase allele of the parent plant and did not hybridize to cDNAs encoding other S-RNase alleles. Two cDNAs for the S2-RNase were cloned from a library derived from anthers of a plant homozygous for the S2 allele and both corresponded to the coding sequence of the S2-RNase. The product of the S-RNase gene was detected by immunocytochemistry in the intine of mature, hydrated pollen grains. These results are interpreted in the light of current knowledge of the structure of the S locus |
| doi_str_mv | 10.1105/tpc.5.12.1771 |
| format | Article |
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Previously, we have shown cosegregation between alleles of the S locus and alleles of a gene that encodes a glycoprotein with ribonuclease activity (S-RNase). Furthermore, expression of the S-RNase gene is apparently confined to the pistil and is correlated with the onset of self-incompatibility. In this paper, we report that the S-RNase gene is also expressed at low levels in developing pollen. A transcript in developing pollen hybridized to a cDNA encoding the S2-RNase allele of the parent plant and did not hybridize to cDNAs encoding other S-RNase alleles. Two cDNAs for the S2-RNase were cloned from a library derived from anthers of a plant homozygous for the S2 allele and both corresponded to the coding sequence of the S2-RNase. The product of the S-RNase gene was detected by immunocytochemistry in the intine of mature, hydrated pollen grains. These results are interpreted in the light of current knowledge of the structure of the S locus</description><identifier>ISSN: 1040-4651</identifier><identifier>EISSN: 1532-298X</identifier><identifier>DOI: 10.1105/tpc.5.12.1771</identifier><identifier>PMID: 8305871</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Alleles ; ANTERA ; ANTHERE ; Anthers ; ARN MENSAJERO ; ARN MESSAGER ; AUTOESTERILIDAD ; AUTOSTERILITE ; Base Sequence ; Biological and medical sciences ; Complementary DNA ; DNA, Complementary - genetics ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GAMETOGENESE ; GAMETOGENESIS ; GENE ; Gene Expression ; GENES ; Genes, Plant ; Genetic loci ; Genotypes ; Immunohistochemistry ; IMMUNOLOGIE ; In Situ Hybridization ; INMUNOLOGIA ; LOCI ; LOCUS ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; NICOTIANA ; Nicotiana - enzymology ; Nicotiana - genetics ; Nicotiana - growth & development ; Plant cells ; Plants, Toxic ; POLEN ; POLLEN ; Pollen - enzymology ; Pollen tubes ; Polymerase Chain Reaction ; RIBONUCLEASAS ; RIBONUCLEASE ; Ribonucleases - genetics ; RNA ; RNA, Messenger - genetics ; SECUENCIA NUCLEICA ; SEQUENCE NUCLEIQUE ; Transcription, Genetic</subject><ispartof>The Plant cell, 1993-12, Vol.5 (12), p.1771-1782</ispartof><rights>Copyright 1993 American Society of Plant Physiologists</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-7181aaa3c278cefcf6ab452a1ecbe3b8515937d406e38334374814a856d4ac823</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3869693$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3869693$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3885153$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8305871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dodds, P.N</creatorcontrib><creatorcontrib>Bonig, I</creatorcontrib><creatorcontrib>Du, H</creatorcontrib><creatorcontrib>Rodin, J</creatorcontrib><creatorcontrib>Anderson, M.A</creatorcontrib><creatorcontrib>Newbigin, E</creatorcontrib><creatorcontrib>Clarke, A.E</creatorcontrib><title>S-RNase gene of Nicotiana alata is expressed in developing pollen</title><title>The Plant cell</title><addtitle>Plant Cell</addtitle><description>In the solanaceous plant Nicotiana alata, self-incompatibility is controlled by a single, multiallelic locus (S locus) expressed in both pollen and pistil. Previously, we have shown cosegregation between alleles of the S locus and alleles of a gene that encodes a glycoprotein with ribonuclease activity (S-RNase). Furthermore, expression of the S-RNase gene is apparently confined to the pistil and is correlated with the onset of self-incompatibility. In this paper, we report that the S-RNase gene is also expressed at low levels in developing pollen. A transcript in developing pollen hybridized to a cDNA encoding the S2-RNase allele of the parent plant and did not hybridize to cDNAs encoding other S-RNase alleles. Two cDNAs for the S2-RNase were cloned from a library derived from anthers of a plant homozygous for the S2 allele and both corresponded to the coding sequence of the S2-RNase. The product of the S-RNase gene was detected by immunocytochemistry in the intine of mature, hydrated pollen grains. These results are interpreted in the light of current knowledge of the structure of the S locus</description><subject>Alleles</subject><subject>ANTERA</subject><subject>ANTHERE</subject><subject>Anthers</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>AUTOESTERILIDAD</subject><subject>AUTOSTERILITE</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Complementary DNA</subject><subject>DNA, Complementary - genetics</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GAMETOGENESE</subject><subject>GAMETOGENESIS</subject><subject>GENE</subject><subject>Gene Expression</subject><subject>GENES</subject><subject>Genes, Plant</subject><subject>Genetic loci</subject><subject>Genotypes</subject><subject>Immunohistochemistry</subject><subject>IMMUNOLOGIE</subject><subject>In Situ Hybridization</subject><subject>INMUNOLOGIA</subject><subject>LOCI</subject><subject>LOCUS</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>NICOTIANA</subject><subject>Nicotiana - enzymology</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - growth & development</subject><subject>Plant cells</subject><subject>Plants, Toxic</subject><subject>POLEN</subject><subject>POLLEN</subject><subject>Pollen - enzymology</subject><subject>Pollen tubes</subject><subject>Polymerase Chain Reaction</subject><subject>RIBONUCLEASAS</subject><subject>RIBONUCLEASE</subject><subject>Ribonucleases - genetics</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>Transcription, Genetic</subject><issn>1040-4651</issn><issn>1532-298X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM9LHDEUx0NRrNoeexEKOfQ6a15-TDIHDyJVC6KgFXoLbzNv1sg4GZJR2v--s-yytacEvp_ve7wPY19ALACEOZ3GsDALkAuwFj6wQzBKVrJxv_bmv9Ci0rWBj-yolGchBFhoDtiBU8I4C4fs_KG6v8VCfEUD8dTx2xjSFHFAjj1OyGPh9HvMVAq1PA68pTfq0xiHFR9T39Pwie132Bf6vH2P2ePl958X19XN3dWPi_ObKugGpsqCA0RUQVoXqAtdjUttJAKFJamlM2AaZVstalJOKa2sdqDRmbrVGJxUx-xsM3d8Xb5QG2iYMvZ-zPEF8x-fMPr_kyE--VV681DPFtTcrzb9kFMpmbpdFYRfm_SzSW88SL82OfNf3-_b0Vt1c_5tm2MJ2HcZhxDLDlNufZL6hz2XKeX3O6USdsbqpm7W2MkG6zB5XOV50uNDo42QqlZ_AekNj-A</recordid><startdate>19931201</startdate><enddate>19931201</enddate><creator>Dodds, P.N</creator><creator>Bonig, I</creator><creator>Du, H</creator><creator>Rodin, J</creator><creator>Anderson, M.A</creator><creator>Newbigin, E</creator><creator>Clarke, A.E</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19931201</creationdate><title>S-RNase gene of Nicotiana alata is expressed in developing pollen</title><author>Dodds, P.N ; Bonig, I ; Du, H ; Rodin, J ; Anderson, M.A ; Newbigin, E ; Clarke, A.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-7181aaa3c278cefcf6ab452a1ecbe3b8515937d406e38334374814a856d4ac823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Alleles</topic><topic>ANTERA</topic><topic>ANTHERE</topic><topic>Anthers</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>AUTOESTERILIDAD</topic><topic>AUTOSTERILITE</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Complementary DNA</topic><topic>DNA, Complementary - genetics</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GAMETOGENESE</topic><topic>GAMETOGENESIS</topic><topic>GENE</topic><topic>Gene Expression</topic><topic>GENES</topic><topic>Genes, Plant</topic><topic>Genetic loci</topic><topic>Genotypes</topic><topic>Immunohistochemistry</topic><topic>IMMUNOLOGIE</topic><topic>In Situ Hybridization</topic><topic>INMUNOLOGIA</topic><topic>LOCI</topic><topic>LOCUS</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>NICOTIANA</topic><topic>Nicotiana - enzymology</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - growth & development</topic><topic>Plant cells</topic><topic>Plants, Toxic</topic><topic>POLEN</topic><topic>POLLEN</topic><topic>Pollen - enzymology</topic><topic>Pollen tubes</topic><topic>Polymerase Chain Reaction</topic><topic>RIBONUCLEASAS</topic><topic>RIBONUCLEASE</topic><topic>Ribonucleases - genetics</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dodds, P.N</creatorcontrib><creatorcontrib>Bonig, I</creatorcontrib><creatorcontrib>Du, H</creatorcontrib><creatorcontrib>Rodin, J</creatorcontrib><creatorcontrib>Anderson, M.A</creatorcontrib><creatorcontrib>Newbigin, E</creatorcontrib><creatorcontrib>Clarke, A.E</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Plant cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dodds, P.N</au><au>Bonig, I</au><au>Du, H</au><au>Rodin, J</au><au>Anderson, M.A</au><au>Newbigin, E</au><au>Clarke, A.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>S-RNase gene of Nicotiana alata is expressed in developing pollen</atitle><jtitle>The Plant cell</jtitle><addtitle>Plant Cell</addtitle><date>1993-12-01</date><risdate>1993</risdate><volume>5</volume><issue>12</issue><spage>1771</spage><epage>1782</epage><pages>1771-1782</pages><issn>1040-4651</issn><eissn>1532-298X</eissn><abstract>In the solanaceous plant Nicotiana alata, self-incompatibility is controlled by a single, multiallelic locus (S locus) expressed in both pollen and pistil. Previously, we have shown cosegregation between alleles of the S locus and alleles of a gene that encodes a glycoprotein with ribonuclease activity (S-RNase). Furthermore, expression of the S-RNase gene is apparently confined to the pistil and is correlated with the onset of self-incompatibility. In this paper, we report that the S-RNase gene is also expressed at low levels in developing pollen. A transcript in developing pollen hybridized to a cDNA encoding the S2-RNase allele of the parent plant and did not hybridize to cDNAs encoding other S-RNase alleles. Two cDNAs for the S2-RNase were cloned from a library derived from anthers of a plant homozygous for the S2 allele and both corresponded to the coding sequence of the S2-RNase. The product of the S-RNase gene was detected by immunocytochemistry in the intine of mature, hydrated pollen grains. These results are interpreted in the light of current knowledge of the structure of the S locus</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>8305871</pmid><doi>10.1105/tpc.5.12.1771</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Plant cell, 1993-12, Vol.5 (12), p.1771-1782 |
| issn | 1040-4651 1532-298X |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Alleles ANTERA ANTHERE Anthers ARN MENSAJERO ARN MESSAGER AUTOESTERILIDAD AUTOSTERILITE Base Sequence Biological and medical sciences Complementary DNA DNA, Complementary - genetics EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GAMETOGENESE GAMETOGENESIS GENE Gene Expression GENES Genes, Plant Genetic loci Genotypes Immunohistochemistry IMMUNOLOGIE In Situ Hybridization INMUNOLOGIA LOCI LOCUS Molecular and cellular biology Molecular genetics Molecular Sequence Data NICOTIANA Nicotiana - enzymology Nicotiana - genetics Nicotiana - growth & development Plant cells Plants, Toxic POLEN POLLEN Pollen - enzymology Pollen tubes Polymerase Chain Reaction RIBONUCLEASAS RIBONUCLEASE Ribonucleases - genetics RNA RNA, Messenger - genetics SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE Transcription, Genetic |
| title | S-RNase gene of Nicotiana alata is expressed in developing pollen |
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