Alanine scanning mutagenesis of a plant virus movement protein identifies three functional domains

Alanine scanning mutagenesis was performed on the red clover necrotic mosaic virus (RCNMV) movement protein (MP), and 12 mutants were assayed in vitro for RNA binding characteristics and in vivo for their ability to potentiate RCNMV cell-to-cell movement. The mutant phenotypes that were identified i...

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Veröffentlicht in:	The Plant cell 1993-08, Vol.5 (8), p.973-982
Hauptverfasser:	Giesman-Cookmeyer, D, Lommel, S.A
Format:	Artikel
Sprache:	eng
Schlagworte:	ALANINA ALANINE Amino Acid Sequence Amino acids ARN Biological and medical sciences Cell Movement - genetics DIANTHOVIRUS Fundamental and applied biological sciences. Psychology Gels Genes, Viral Genetic mutation Leaves Microbiology Molecular Sequence Data Mosaic Viruses - chemistry Mosaic Viruses - genetics Mosaic Viruses - physiology MUTACION MUTACION INDUCIDA Mutagenesis, Site-Directed MUTATION MUTATION PROVOQUEE NICOTIANA PATHOGENESE PATOGENESIS Plant cells Plant Viral Movement Proteins Plasmodesmata Protein Structure, Tertiary PROTEINAS PROTEINAS AGLUTINANTES PROTEINE PROTEINE DE LIAISON Proteins Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains RNA RNA probes RNA, Viral - genetics RNA, Viral - metabolism RNA-Binding Proteins - chemistry RNA-Binding Proteins - genetics RNA-Binding Proteins - metabolism Viral Proteins - chemistry Viral Proteins - genetics Viral Proteins - metabolism Virology VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX Viruses
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