An Arabidopsis mutant defective in the general phenylpropanoid pathway
Mutants of Arabidopsis deficient in a major leaf phenylpropanoid ester, 2-O-sinapoyl-L-malate, were identified by thin-layer chromatographic screening of methanolic leaf extracts from several thousand mutagenized plants. Mutations at a locus designated SIN1 also eliminate accumulation of the sinapic...
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| Veröffentlicht in: | The Plant cell 1992-11, Vol.4 (11), p.1413-1424 |
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| container_title | The Plant cell |
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| creator | Chapple, C.C.S. (Michigan State University, East Lansing, MI) Vogt, T Ellis, B.E Somerville, C.R |
| description | Mutants of Arabidopsis deficient in a major leaf phenylpropanoid ester, 2-O-sinapoyl-L-malate, were identified by thin-layer chromatographic screening of methanolic leaf extracts from several thousand mutagenized plants. Mutations at a locus designated SIN1 also eliminate accumulation of the sinapic acid esters characteristic of seed tissues. Because of increased transparency to UV light, the sin1 mutants exhibit a characteristic red fluorescence under UV light, whereas wild-type plants have a blue-green appearance due to the fluorescence of sinapoyl malate in the upper epidermis. As determined by in vivo radiotracer feeding experiments, precursor supplementation studies, and enzymatic assays, the defect in the sin1 mutants appears to block the conversion of ferulate to 5-hydroxyferulate in the general phenylpropanoid pathway. As a result, the lignin of the mutant lacks the sinapic acid-derived components typical of wild-type lignin |
| doi_str_mv | 10.1105/tpc.4.11.1413 |
| format | Article |
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(Michigan State University, East Lansing, MI) ; Vogt, T ; Ellis, B.E ; Somerville, C.R</creator><creatorcontrib>Chapple, C.C.S. (Michigan State University, East Lansing, MI) ; Vogt, T ; Ellis, B.E ; Somerville, C.R</creatorcontrib><description>Mutants of Arabidopsis deficient in a major leaf phenylpropanoid ester, 2-O-sinapoyl-L-malate, were identified by thin-layer chromatographic screening of methanolic leaf extracts from several thousand mutagenized plants. Mutations at a locus designated SIN1 also eliminate accumulation of the sinapic acid esters characteristic of seed tissues. Because of increased transparency to UV light, the sin1 mutants exhibit a characteristic red fluorescence under UV light, whereas wild-type plants have a blue-green appearance due to the fluorescence of sinapoyl malate in the upper epidermis. As determined by in vivo radiotracer feeding experiments, precursor supplementation studies, and enzymatic assays, the defect in the sin1 mutants appears to block the conversion of ferulate to 5-hydroxyferulate in the general phenylpropanoid pathway. As a result, the lignin of the mutant lacks the sinapic acid-derived components typical of wild-type lignin</description><identifier>ISSN: 1040-4651</identifier><identifier>EISSN: 1532-298X</identifier><identifier>DOI: 10.1105/tpc.4.11.1413</identifier><identifier>PMID: 1477555</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ACIDE ORGANIQUE ; ACIDE PHENOLIQUE ; ACIDOS FENOLICOS ; ACIDOS ORGANICOS ; Arabidopsis - chemistry ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Biological and medical sciences ; Biosynthesis ; Choline - analogs & derivatives ; Choline - metabolism ; Cinnamates - metabolism ; Classical genetics, quantitative genetics, hybrids ; COMPOSE AROMATIQUE ; COMPUESTOS AROMATICOS ; Coumaric acids ; Coumaric Acids - isolation & purification ; Coumaric Acids - metabolism ; CRUCIFERAE ; Enzymes ; ESTER ; ESTERES ; Esters ; Esters - isolation & purification ; Esters - metabolism ; FEUILLE ; FITOALEXINA ; Fluorescence ; Fundamental and applied biological sciences. Psychology ; Genes, Plant - genetics ; Genetic loci ; Genetic mutation ; Genetics of eukaryotes. Biological and molecular evolution ; Histocytochemistry ; HOJAS ; Leaves ; Lignin ; Lignin - analysis ; LIGNINAS ; LIGNINE ; LOCI ; LOCUS ; Malates - isolation & purification ; Malates - metabolism ; METABOLISME ; METABOLISMO ; MUTACION INDUCIDA ; Mutagenesis ; MUTANT ; MUTANTES ; MUTATION PROVOQUEE ; Phenylpropionates - isolation & purification ; Phenylpropionates - metabolism ; PHYTOALEXINE ; Plants ; PROPIEDADES OPTICAS ; PROPRIETE OPTIQUE ; Pteridophyta, spermatophyta ; RADIACION ULTRAVIOLETA ; RAYONNEMENT ULTRAVIOLET ; Seedlings ; Seeds - chemistry ; SINAPINA ; SINAPINE ; Vegetals</subject><ispartof>The Plant cell, 1992-11, Vol.4 (11), p.1413-1424</ispartof><rights>Copyright 1992 American Society of Plant Physiologists</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-4f1a542748eb91d1e47638a9d4db738dcd69ff764bbe1ef7e8eb4e94f9318f203</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3869512$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3869512$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,778,782,801,883,27913,27914,58006,58239</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4462929$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1477555$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chapple, C.C.S. (Michigan State University, East Lansing, MI)</creatorcontrib><creatorcontrib>Vogt, T</creatorcontrib><creatorcontrib>Ellis, B.E</creatorcontrib><creatorcontrib>Somerville, C.R</creatorcontrib><title>An Arabidopsis mutant defective in the general phenylpropanoid pathway</title><title>The Plant cell</title><addtitle>Plant Cell</addtitle><description>Mutants of Arabidopsis deficient in a major leaf phenylpropanoid ester, 2-O-sinapoyl-L-malate, were identified by thin-layer chromatographic screening of methanolic leaf extracts from several thousand mutagenized plants. Mutations at a locus designated SIN1 also eliminate accumulation of the sinapic acid esters characteristic of seed tissues. Because of increased transparency to UV light, the sin1 mutants exhibit a characteristic red fluorescence under UV light, whereas wild-type plants have a blue-green appearance due to the fluorescence of sinapoyl malate in the upper epidermis. As determined by in vivo radiotracer feeding experiments, precursor supplementation studies, and enzymatic assays, the defect in the sin1 mutants appears to block the conversion of ferulate to 5-hydroxyferulate in the general phenylpropanoid pathway. As a result, the lignin of the mutant lacks the sinapic acid-derived components typical of wild-type lignin</description><subject>ACIDE ORGANIQUE</subject><subject>ACIDE PHENOLIQUE</subject><subject>ACIDOS FENOLICOS</subject><subject>ACIDOS ORGANICOS</subject><subject>Arabidopsis - chemistry</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Choline - analogs & derivatives</subject><subject>Choline - metabolism</subject><subject>Cinnamates - metabolism</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>COMPOSE AROMATIQUE</subject><subject>COMPUESTOS AROMATICOS</subject><subject>Coumaric acids</subject><subject>Coumaric Acids - isolation & purification</subject><subject>Coumaric Acids - metabolism</subject><subject>CRUCIFERAE</subject><subject>Enzymes</subject><subject>ESTER</subject><subject>ESTERES</subject><subject>Esters</subject><subject>Esters - isolation & purification</subject><subject>Esters - metabolism</subject><subject>FEUILLE</subject><subject>FITOALEXINA</subject><subject>Fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Plant - genetics</subject><subject>Genetic loci</subject><subject>Genetic mutation</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Histocytochemistry</subject><subject>HOJAS</subject><subject>Leaves</subject><subject>Lignin</subject><subject>Lignin - analysis</subject><subject>LIGNINAS</subject><subject>LIGNINE</subject><subject>LOCI</subject><subject>LOCUS</subject><subject>Malates - isolation & purification</subject><subject>Malates - metabolism</subject><subject>METABOLISME</subject><subject>METABOLISMO</subject><subject>MUTACION INDUCIDA</subject><subject>Mutagenesis</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>MUTATION PROVOQUEE</subject><subject>Phenylpropionates - isolation & purification</subject><subject>Phenylpropionates - metabolism</subject><subject>PHYTOALEXINE</subject><subject>Plants</subject><subject>PROPIEDADES OPTICAS</subject><subject>PROPRIETE OPTIQUE</subject><subject>Pteridophyta, spermatophyta</subject><subject>RADIACION ULTRAVIOLETA</subject><subject>RAYONNEMENT ULTRAVIOLET</subject><subject>Seedlings</subject><subject>Seeds - chemistry</subject><subject>SINAPINA</subject><subject>SINAPINE</subject><subject>Vegetals</subject><issn>1040-4651</issn><issn>1532-298X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkL1rHDEQxUWwsR0nZZqAYYu0e9boY7UqXBwmTgwGF4khndCuRncyd9pFkh3uv7fMGX9U8-D93szwCPkGdAFA5XmZx4WocgEC-CdyApKzlun-30HVVNBWdBKOyeec7ymloEAfkSMQSkkpT8jVMjbLZIfgpjmH3Gwfio2lcehxLOERmxCbssZmhRGT3TTzGuNuM6dptnEKrpltWf-3uy_k0NtNxq8v85TcXf38e_m7vbn9dX25vGlHKVVphQcrBVOix0GDAxSq473VTrhB8d6NrtPeq04MAwJ6hZUTqIXXHHrPKD8lF_u988OwRTdiLPUrM6ewtWlnJhvMRyeGtVlNjwY6ylhf8-0-P6Yp54T-NQrUPNdpap1GVGme66z82ft7b_S-v-r_ePFtHu3GJxvHkF8xITqmmX7D7nOZ0vubjFNleN9pCaxi3_eYt5Oxq1Q33f3RnDGqKH8Cn3mTgw</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>Chapple, C.C.S. (Michigan State University, East Lansing, MI)</creator><creator>Vogt, T</creator><creator>Ellis, B.E</creator><creator>Somerville, C.R</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>5PM</scope></search><sort><creationdate>19921101</creationdate><title>An Arabidopsis mutant defective in the general phenylpropanoid pathway</title><author>Chapple, C.C.S. (Michigan State University, East Lansing, MI) ; Vogt, T ; Ellis, B.E ; Somerville, C.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-4f1a542748eb91d1e47638a9d4db738dcd69ff764bbe1ef7e8eb4e94f9318f203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ACIDE ORGANIQUE</topic><topic>ACIDE PHENOLIQUE</topic><topic>ACIDOS FENOLICOS</topic><topic>ACIDOS ORGANICOS</topic><topic>Arabidopsis - chemistry</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Choline - analogs & derivatives</topic><topic>Choline - metabolism</topic><topic>Cinnamates - metabolism</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>COMPOSE AROMATIQUE</topic><topic>COMPUESTOS AROMATICOS</topic><topic>Coumaric acids</topic><topic>Coumaric Acids - isolation & purification</topic><topic>Coumaric Acids - metabolism</topic><topic>CRUCIFERAE</topic><topic>Enzymes</topic><topic>ESTER</topic><topic>ESTERES</topic><topic>Esters</topic><topic>Esters - isolation & purification</topic><topic>Esters - metabolism</topic><topic>FEUILLE</topic><topic>FITOALEXINA</topic><topic>Fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Plant - genetics</topic><topic>Genetic loci</topic><topic>Genetic mutation</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Histocytochemistry</topic><topic>HOJAS</topic><topic>Leaves</topic><topic>Lignin</topic><topic>Lignin - analysis</topic><topic>LIGNINAS</topic><topic>LIGNINE</topic><topic>LOCI</topic><topic>LOCUS</topic><topic>Malates - isolation & purification</topic><topic>Malates - metabolism</topic><topic>METABOLISME</topic><topic>METABOLISMO</topic><topic>MUTACION INDUCIDA</topic><topic>Mutagenesis</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>MUTATION PROVOQUEE</topic><topic>Phenylpropionates - isolation & purification</topic><topic>Phenylpropionates - metabolism</topic><topic>PHYTOALEXINE</topic><topic>Plants</topic><topic>PROPIEDADES OPTICAS</topic><topic>PROPRIETE OPTIQUE</topic><topic>Pteridophyta, spermatophyta</topic><topic>RADIACION ULTRAVIOLETA</topic><topic>RAYONNEMENT ULTRAVIOLET</topic><topic>Seedlings</topic><topic>Seeds - chemistry</topic><topic>SINAPINA</topic><topic>SINAPINE</topic><topic>Vegetals</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chapple, C.C.S. (Michigan State University, East Lansing, MI)</creatorcontrib><creatorcontrib>Vogt, T</creatorcontrib><creatorcontrib>Ellis, B.E</creatorcontrib><creatorcontrib>Somerville, C.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Plant cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chapple, C.C.S. (Michigan State University, East Lansing, MI)</au><au>Vogt, T</au><au>Ellis, B.E</au><au>Somerville, C.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Arabidopsis mutant defective in the general phenylpropanoid pathway</atitle><jtitle>The Plant cell</jtitle><addtitle>Plant Cell</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>4</volume><issue>11</issue><spage>1413</spage><epage>1424</epage><pages>1413-1424</pages><issn>1040-4651</issn><eissn>1532-298X</eissn><abstract>Mutants of Arabidopsis deficient in a major leaf phenylpropanoid ester, 2-O-sinapoyl-L-malate, were identified by thin-layer chromatographic screening of methanolic leaf extracts from several thousand mutagenized plants. Mutations at a locus designated SIN1 also eliminate accumulation of the sinapic acid esters characteristic of seed tissues. Because of increased transparency to UV light, the sin1 mutants exhibit a characteristic red fluorescence under UV light, whereas wild-type plants have a blue-green appearance due to the fluorescence of sinapoyl malate in the upper epidermis. As determined by in vivo radiotracer feeding experiments, precursor supplementation studies, and enzymatic assays, the defect in the sin1 mutants appears to block the conversion of ferulate to 5-hydroxyferulate in the general phenylpropanoid pathway. As a result, the lignin of the mutant lacks the sinapic acid-derived components typical of wild-type lignin</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>1477555</pmid><doi>10.1105/tpc.4.11.1413</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Plant cell, 1992-11, Vol.4 (11), p.1413-1424 |
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| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Jstor Complete Legacy; Alma/SFX Local Collection |
| subjects | ACIDE ORGANIQUE ACIDE PHENOLIQUE ACIDOS FENOLICOS ACIDOS ORGANICOS Arabidopsis - chemistry Arabidopsis - genetics Arabidopsis - metabolism Biological and medical sciences Biosynthesis Choline - analogs & derivatives Choline - metabolism Cinnamates - metabolism Classical genetics, quantitative genetics, hybrids COMPOSE AROMATIQUE COMPUESTOS AROMATICOS Coumaric acids Coumaric Acids - isolation & purification Coumaric Acids - metabolism CRUCIFERAE Enzymes ESTER ESTERES Esters Esters - isolation & purification Esters - metabolism FEUILLE FITOALEXINA Fluorescence Fundamental and applied biological sciences. Psychology Genes, Plant - genetics Genetic loci Genetic mutation Genetics of eukaryotes. Biological and molecular evolution Histocytochemistry HOJAS Leaves Lignin Lignin - analysis LIGNINAS LIGNINE LOCI LOCUS Malates - isolation & purification Malates - metabolism METABOLISME METABOLISMO MUTACION INDUCIDA Mutagenesis MUTANT MUTANTES MUTATION PROVOQUEE Phenylpropionates - isolation & purification Phenylpropionates - metabolism PHYTOALEXINE Plants PROPIEDADES OPTICAS PROPRIETE OPTIQUE Pteridophyta, spermatophyta RADIACION ULTRAVIOLETA RAYONNEMENT ULTRAVIOLET Seedlings Seeds - chemistry SINAPINA SINAPINE Vegetals |
| title | An Arabidopsis mutant defective in the general phenylpropanoid pathway |
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