Isolation of the Arabidopsis ABI3 gene by positional cloning
Arabidopsis abi3 mutants are altered in various aspects of seed development and germination that reflect a decreased responsiveness to the hormone abscisic acid. The ABI3 gene has been isolated by positional cloning. A detailed restriction fragment length polymorphism (RFLP) map of the abi3 region w...
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| Veröffentlicht in: | The Plant cell 1992-10, Vol.4 (10), p.1251-1261 |
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| container_title | The Plant cell |
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| creator | Giraudat, J. (Harvard Medical School, Boston, MA) Hauge, B.M Valon, C Smalle, J Parcy, F Goodman, H.M |
| description | Arabidopsis abi3 mutants are altered in various aspects of seed development and germination that reflect a decreased responsiveness to the hormone abscisic acid. The ABI3 gene has been isolated by positional cloning. A detailed restriction fragment length polymorphism (RFLP) map of the abi3 region was constructed. An RFLP marker closely linked to the abi3 locus was identified, and by analyzing an overlapping set of cosmid clones containing this marker, the abi3 locus was localized within a 35-kb region. An 11-kb subfragment was then shown to complement the mutant phenotype in transgenic plants, thereby further delimiting the position of the locus. A candidate ABI3 gene was identified within this fragment as being expressed in developing fruits. The primary structure of the encoded protein was deduced from sequence analysis of a corresponding cDNA clone. In the most severe abi3-4 allele, the size of this predicted protein was reduced by 40% due to the presence of a point mutation that introduced a premature stop codon. The predicted ABI3 protein displays discrete regions of high similarity to the maize viviparous-1 protein |
| doi_str_mv | 10.1105/tpc.4.10.1251 |
| format | Article |
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(Harvard Medical School, Boston, MA) ; Hauge, B.M ; Valon, C ; Smalle, J ; Parcy, F ; Goodman, H.M</creator><creatorcontrib>Giraudat, J. (Harvard Medical School, Boston, MA) ; Hauge, B.M ; Valon, C ; Smalle, J ; Parcy, F ; Goodman, H.M</creatorcontrib><description>Arabidopsis abi3 mutants are altered in various aspects of seed development and germination that reflect a decreased responsiveness to the hormone abscisic acid. The ABI3 gene has been isolated by positional cloning. A detailed restriction fragment length polymorphism (RFLP) map of the abi3 region was constructed. An RFLP marker closely linked to the abi3 locus was identified, and by analyzing an overlapping set of cosmid clones containing this marker, the abi3 locus was localized within a 35-kb region. An 11-kb subfragment was then shown to complement the mutant phenotype in transgenic plants, thereby further delimiting the position of the locus. A candidate ABI3 gene was identified within this fragment as being expressed in developing fruits. The primary structure of the encoded protein was deduced from sequence analysis of a corresponding cDNA clone. In the most severe abi3-4 allele, the size of this predicted protein was reduced by 40% due to the presence of a point mutation that introduced a premature stop codon. The predicted ABI3 protein displays discrete regions of high similarity to the maize viviparous-1 protein</description><identifier>ISSN: 1040-4651</identifier><identifier>EISSN: 1532-298X</identifier><identifier>DOI: 10.1105/tpc.4.10.1251</identifier><identifier>PMID: 1359917</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ADN ; Alleles ; Amino Acid Sequence ; Arabidopsis - genetics ; Arabidopsis Proteins ; Biological and medical sciences ; Chromosome Mapping ; CLONACION ; CLONAGE ; Cloning, Molecular - methods ; Complementary DNA ; Corn ; Cosmids ; CRUCIFERAE ; DNA ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genes, Overlapping ; Genes, Plant ; Genes. Genome ; Genetic Complementation Test ; Genetic Linkage ; Genetic loci ; Genetic Markers ; Genetic mutation ; GENETICA ; GENETIQUE ; Genomics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; MUTACION ; MUTATION ; Phenotypes ; Plant Proteins - genetics ; POLIMORFISMO ; Polymorphism, Restriction Fragment Length ; POLYMORPHISME ; PROTEINAS VEGETALES ; PROTEINE VEGETALE ; SECUENCIA NUCLEICA ; SEGREGACION ; SEGREGATION ; SEQUENCE NUCLEIQUE ; Transcription Factors</subject><ispartof>The Plant cell, 1992-10, Vol.4 (10), p.1251-1261</ispartof><rights>Copyright 1992 American Society of Plant Physiologists</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-a273ad19e7c298ef85159b24160a72b27d5690c76cc081f328a1cfd21e616ed73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3869411$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3869411$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4334381$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1359917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Giraudat, J. (Harvard Medical School, Boston, MA)</creatorcontrib><creatorcontrib>Hauge, B.M</creatorcontrib><creatorcontrib>Valon, C</creatorcontrib><creatorcontrib>Smalle, J</creatorcontrib><creatorcontrib>Parcy, F</creatorcontrib><creatorcontrib>Goodman, H.M</creatorcontrib><title>Isolation of the Arabidopsis ABI3 gene by positional cloning</title><title>The Plant cell</title><addtitle>Plant Cell</addtitle><description>Arabidopsis abi3 mutants are altered in various aspects of seed development and germination that reflect a decreased responsiveness to the hormone abscisic acid. The ABI3 gene has been isolated by positional cloning. A detailed restriction fragment length polymorphism (RFLP) map of the abi3 region was constructed. An RFLP marker closely linked to the abi3 locus was identified, and by analyzing an overlapping set of cosmid clones containing this marker, the abi3 locus was localized within a 35-kb region. An 11-kb subfragment was then shown to complement the mutant phenotype in transgenic plants, thereby further delimiting the position of the locus. A candidate ABI3 gene was identified within this fragment as being expressed in developing fruits. The primary structure of the encoded protein was deduced from sequence analysis of a corresponding cDNA clone. In the most severe abi3-4 allele, the size of this predicted protein was reduced by 40% due to the presence of a point mutation that introduced a premature stop codon. The predicted ABI3 protein displays discrete regions of high similarity to the maize viviparous-1 protein</description><subject>ADN</subject><subject>Alleles</subject><subject>Amino Acid Sequence</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>Cloning, Molecular - methods</subject><subject>Complementary DNA</subject><subject>Corn</subject><subject>Cosmids</subject><subject>CRUCIFERAE</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Overlapping</subject><subject>Genes, Plant</subject><subject>Genes. Genome</subject><subject>Genetic Complementation Test</subject><subject>Genetic Linkage</subject><subject>Genetic loci</subject><subject>Genetic Markers</subject><subject>Genetic mutation</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>Genomics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>MUTACION</subject><subject>MUTATION</subject><subject>Phenotypes</subject><subject>Plant Proteins - genetics</subject><subject>POLIMORFISMO</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>POLYMORPHISME</subject><subject>PROTEINAS VEGETALES</subject><subject>PROTEINE VEGETALE</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEGREGACION</subject><subject>SEGREGATION</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>Transcription Factors</subject><issn>1040-4651</issn><issn>1532-298X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM1r3DAQxUVoSJO0x1wKBR9Cbt5qJEuyoDlsQj4WAj20gd7EWJY3Cl7LlbyB_PfRdpd86DIa3m_mDY-QE6AzACp-TKOdVbNNxwTskUMQnJVM138_5T-taFlJAZ_JUUqPlFJQoA_IAXChNahD8nORQo-TD0MRumJ6cMU8YuPbMCafivnFghdLN7iieS7GkPwGxL6wfRj8sPxC9jvsk_u6q8fk_vrqz-VteffrZnE5vyutEGoqkSmOLWinbL7LdbUAoRtWgaSoWMNUK6SmVklraQ0dZzWC7VoGToJ0reLH5Hy7d1w3K9daN0wRezNGv8L4bAJ681EZ_INZhieTHRiwPH-2m4_h39qlyax8sq7vcXBhnYzi-TGpM1huQRtDStF1rx5AzSZtk9M21f8up5357-8Pe6O38Wb9dKdjsth3EQfr0ytWcV7xGt6wxzSF-N6TcaoMr6WuYIN922IdBoPLmDfd_9YctKw5fwHEZZsM</recordid><startdate>19921001</startdate><enddate>19921001</enddate><creator>Giraudat, J. (Harvard Medical School, Boston, MA)</creator><creator>Hauge, B.M</creator><creator>Valon, C</creator><creator>Smalle, J</creator><creator>Parcy, F</creator><creator>Goodman, H.M</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19921001</creationdate><title>Isolation of the Arabidopsis ABI3 gene by positional cloning</title><author>Giraudat, J. (Harvard Medical School, Boston, MA) ; Hauge, B.M ; Valon, C ; Smalle, J ; Parcy, F ; Goodman, H.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-a273ad19e7c298ef85159b24160a72b27d5690c76cc081f328a1cfd21e616ed73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ADN</topic><topic>Alleles</topic><topic>Amino Acid Sequence</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>Cloning, Molecular - methods</topic><topic>Complementary DNA</topic><topic>Corn</topic><topic>Cosmids</topic><topic>CRUCIFERAE</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Overlapping</topic><topic>Genes, Plant</topic><topic>Genes. Genome</topic><topic>Genetic Complementation Test</topic><topic>Genetic Linkage</topic><topic>Genetic loci</topic><topic>Genetic Markers</topic><topic>Genetic mutation</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>Genomics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>MUTACION</topic><topic>MUTATION</topic><topic>Phenotypes</topic><topic>Plant Proteins - genetics</topic><topic>POLIMORFISMO</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>POLYMORPHISME</topic><topic>PROTEINAS VEGETALES</topic><topic>PROTEINE VEGETALE</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEGREGACION</topic><topic>SEGREGATION</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>Transcription Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giraudat, J. (Harvard Medical School, Boston, MA)</creatorcontrib><creatorcontrib>Hauge, B.M</creatorcontrib><creatorcontrib>Valon, C</creatorcontrib><creatorcontrib>Smalle, J</creatorcontrib><creatorcontrib>Parcy, F</creatorcontrib><creatorcontrib>Goodman, H.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Plant cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giraudat, J. (Harvard Medical School, Boston, MA)</au><au>Hauge, B.M</au><au>Valon, C</au><au>Smalle, J</au><au>Parcy, F</au><au>Goodman, H.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of the Arabidopsis ABI3 gene by positional cloning</atitle><jtitle>The Plant cell</jtitle><addtitle>Plant Cell</addtitle><date>1992-10-01</date><risdate>1992</risdate><volume>4</volume><issue>10</issue><spage>1251</spage><epage>1261</epage><pages>1251-1261</pages><issn>1040-4651</issn><eissn>1532-298X</eissn><abstract>Arabidopsis abi3 mutants are altered in various aspects of seed development and germination that reflect a decreased responsiveness to the hormone abscisic acid. The ABI3 gene has been isolated by positional cloning. A detailed restriction fragment length polymorphism (RFLP) map of the abi3 region was constructed. An RFLP marker closely linked to the abi3 locus was identified, and by analyzing an overlapping set of cosmid clones containing this marker, the abi3 locus was localized within a 35-kb region. An 11-kb subfragment was then shown to complement the mutant phenotype in transgenic plants, thereby further delimiting the position of the locus. A candidate ABI3 gene was identified within this fragment as being expressed in developing fruits. The primary structure of the encoded protein was deduced from sequence analysis of a corresponding cDNA clone. In the most severe abi3-4 allele, the size of this predicted protein was reduced by 40% due to the presence of a point mutation that introduced a premature stop codon. The predicted ABI3 protein displays discrete regions of high similarity to the maize viviparous-1 protein</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>1359917</pmid><doi>10.1105/tpc.4.10.1251</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1040-4651 |
| ispartof | The Plant cell, 1992-10, Vol.4 (10), p.1251-1261 |
| issn | 1040-4651 1532-298X |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | ADN Alleles Amino Acid Sequence Arabidopsis - genetics Arabidopsis Proteins Biological and medical sciences Chromosome Mapping CLONACION CLONAGE Cloning, Molecular - methods Complementary DNA Corn Cosmids CRUCIFERAE DNA Fundamental and applied biological sciences. Psychology GENE GENES Genes, Overlapping Genes, Plant Genes. Genome Genetic Complementation Test Genetic Linkage Genetic loci Genetic Markers Genetic mutation GENETICA GENETIQUE Genomics Molecular and cellular biology Molecular genetics Molecular Sequence Data MUTACION MUTATION Phenotypes Plant Proteins - genetics POLIMORFISMO Polymorphism, Restriction Fragment Length POLYMORPHISME PROTEINAS VEGETALES PROTEINE VEGETALE SECUENCIA NUCLEICA SEGREGACION SEGREGATION SEQUENCE NUCLEIQUE Transcription Factors |
| title | Isolation of the Arabidopsis ABI3 gene by positional cloning |
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