A geminivirus replication protein is a sequence-specific DNA binding protein
The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two circular DNA molecules designated as components A and B. The A component encodes the only viral protein, AL1, that is required for viral replication. We showed that AL1 interacts specifically with TGMV A and B DNA by usi...
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| Veröffentlicht in: | The Plant cell 1992-05, Vol.4 (5), p.597-608 |
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| creator | Fontes, E.P.B. (North Carolina State University, Raleigh, NC) Lucknow, V.A Hanley-Bowdoin, L |
| description | The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two circular DNA molecules designated as components A and B. The A component encodes the only viral protein, AL1, that is required for viral replication. We showed that AL1 interacts specifically with TGMV A and B DNA by using an immunoprecipitation assay for AL1:DNA complex formation. In this assay, a monoclonal antibody against All precipitated AL1:TGMV DNA complexes, whereas an unrelated antibody failed to precipitate the complexes. Competition assays with homologous and heterologous DNAs established the specificity of AL1:DNA binding. All produced by transgenic tobacco plants and by baculovirus-infected insect cells exhibited similar DNA binding activity. The All binding site maps to 52 bp on the left side of the common region, a 235-bp region that is highly conserved between the two TGMV genome components. The AL1:DNA binding site does not include the putative hairpin structure that is conserved in the common regions or the equivalent 5' intergenic regions of all geminiviruses. These studies demonstrate that a geminivirus replication protein is a sequence-specific DNA binding protein, and the studies have important implications for the role of this protein in virus replication |
| doi_str_mv | 10.1105/tpc.4.5.597 |
| format | Article |
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(North Carolina State University, Raleigh, NC) ; Lucknow, V.A ; Hanley-Bowdoin, L</creator><creatorcontrib>Fontes, E.P.B. (North Carolina State University, Raleigh, NC) ; Lucknow, V.A ; Hanley-Bowdoin, L</creatorcontrib><description>The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two circular DNA molecules designated as components A and B. The A component encodes the only viral protein, AL1, that is required for viral replication. We showed that AL1 interacts specifically with TGMV A and B DNA by using an immunoprecipitation assay for AL1:DNA complex formation. In this assay, a monoclonal antibody against All precipitated AL1:TGMV DNA complexes, whereas an unrelated antibody failed to precipitate the complexes. Competition assays with homologous and heterologous DNAs established the specificity of AL1:DNA binding. All produced by transgenic tobacco plants and by baculovirus-infected insect cells exhibited similar DNA binding activity. The All binding site maps to 52 bp on the left side of the common region, a 235-bp region that is highly conserved between the two TGMV genome components. The AL1:DNA binding site does not include the putative hairpin structure that is conserved in the common regions or the equivalent 5' intergenic regions of all geminiviruses. These studies demonstrate that a geminivirus replication protein is a sequence-specific DNA binding protein, and the studies have important implications for the role of this protein in virus replication</description><identifier>ISSN: 1040-4651</identifier><identifier>EISSN: 1532-298X</identifier><identifier>DOI: 10.1105/tpc.4.5.597</identifier><identifier>PMID: 1498611</identifier><language>eng</language><publisher>United States: American Society of Plant Physiologists</publisher><subject>ADN ; Base Sequence ; Binding Sites ; Binding, Competitive ; DNA ; DNA probes ; DNA, Viral - metabolism ; DNA-Binding Proteins - metabolism ; Electrophoresis, Polyacrylamide Gel ; Geminiviridae ; GEMINIVIRUS ; GENETICA ; GENETIQUE ; GENOMAS ; GENOME ; Genomes ; Insect genetics ; Insect viruses ; Molecular Sequence Data ; Mosaic viruses ; Mosaic Viruses - metabolism ; Mosaic Viruses - physiology ; Mosaic Viruses - ultrastructure ; NUCLEOTIDE ; NUCLEOTIDOS ; Plants, Genetically Modified ; PROTEINAS ; PROTEINE ; Transgenic insects ; Transgenic plants ; Viral Proteins - metabolism ; VIRUS DE LAS PLANTAS ; VIRUS DES VEGETAUX ; Virus Replication</subject><ispartof>The Plant cell, 1992-05, Vol.4 (5), p.597-608</ispartof><rights>Copyright 1992 American Society of Plant Physiologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-7133660e6865a0476dca64386ad3eaccb02165f3c6034c5dc914eff6fa601813</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3869558$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3869558$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1498611$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fontes, E.P.B. (North Carolina State University, Raleigh, NC)</creatorcontrib><creatorcontrib>Lucknow, V.A</creatorcontrib><creatorcontrib>Hanley-Bowdoin, L</creatorcontrib><title>A geminivirus replication protein is a sequence-specific DNA binding protein</title><title>The Plant cell</title><addtitle>Plant Cell</addtitle><description>The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two circular DNA molecules designated as components A and B. The A component encodes the only viral protein, AL1, that is required for viral replication. We showed that AL1 interacts specifically with TGMV A and B DNA by using an immunoprecipitation assay for AL1:DNA complex formation. In this assay, a monoclonal antibody against All precipitated AL1:TGMV DNA complexes, whereas an unrelated antibody failed to precipitate the complexes. Competition assays with homologous and heterologous DNAs established the specificity of AL1:DNA binding. All produced by transgenic tobacco plants and by baculovirus-infected insect cells exhibited similar DNA binding activity. The All binding site maps to 52 bp on the left side of the common region, a 235-bp region that is highly conserved between the two TGMV genome components. The AL1:DNA binding site does not include the putative hairpin structure that is conserved in the common regions or the equivalent 5' intergenic regions of all geminiviruses. These studies demonstrate that a geminivirus replication protein is a sequence-specific DNA binding protein, and the studies have important implications for the role of this protein in virus replication</description><subject>ADN</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Binding, Competitive</subject><subject>DNA</subject><subject>DNA probes</subject><subject>DNA, Viral - metabolism</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Geminiviridae</subject><subject>GEMINIVIRUS</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>GENOMAS</subject><subject>GENOME</subject><subject>Genomes</subject><subject>Insect genetics</subject><subject>Insect viruses</subject><subject>Molecular Sequence Data</subject><subject>Mosaic viruses</subject><subject>Mosaic Viruses - metabolism</subject><subject>Mosaic Viruses - physiology</subject><subject>Mosaic Viruses - ultrastructure</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDOS</subject><subject>Plants, Genetically Modified</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Transgenic insects</subject><subject>Transgenic plants</subject><subject>Viral Proteins - metabolism</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DES VEGETAUX</subject><subject>Virus Replication</subject><issn>1040-4651</issn><issn>1532-298X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEtLxDAUhYMoPkZX7gShC3EjHXObR5uFi8E3DLpQwV3IpOkY6SQ16Qj-eyMdX6t74XznPg5C-4DHAJid9p0e0zEbM1GuoW1gpMgLUT2vpx5TnFPOYAvtxPiKMYYSxCbaBCoqDrCNppNsbhbW2XcbljELpmutVr31LuuC7411mY2ZyqJ5WxqnTR47o21jdXZxN8lm1tXWzb_RXbTRqDaavVUdocery8fzm3x6f317PpnmmjLa5yUQwjk2vOJMYVryWitOScVVTYzSeoYL4KwhmmNCNau1AGqahjeKY6iAjNDZMLZbzham1sb1QbWyC3ahwof0ysr_irMvcu7fJSQ_48l_vPIHn76KvVzYqE3bKmf8MsqSAADlIoEnA6iDjzGY5mcHYPkVvUzRSyqZTNEn-vDvWb_skHXSjwb9NfY-_B1VEFzK9L9grErYwYA1yks1DzbKpwdBcCEEJp8rwZQ4</recordid><startdate>19920501</startdate><enddate>19920501</enddate><creator>Fontes, E.P.B. 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(North Carolina State University, Raleigh, NC) ; Lucknow, V.A ; Hanley-Bowdoin, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-7133660e6865a0476dca64386ad3eaccb02165f3c6034c5dc914eff6fa601813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ADN</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Binding, Competitive</topic><topic>DNA</topic><topic>DNA probes</topic><topic>DNA, Viral - metabolism</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Geminiviridae</topic><topic>GEMINIVIRUS</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>GENOMAS</topic><topic>GENOME</topic><topic>Genomes</topic><topic>Insect genetics</topic><topic>Insect viruses</topic><topic>Molecular Sequence Data</topic><topic>Mosaic viruses</topic><topic>Mosaic Viruses - metabolism</topic><topic>Mosaic Viruses - physiology</topic><topic>Mosaic Viruses - ultrastructure</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDOS</topic><topic>Plants, Genetically Modified</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Transgenic insects</topic><topic>Transgenic plants</topic><topic>Viral Proteins - metabolism</topic><topic>VIRUS DE LAS PLANTAS</topic><topic>VIRUS DES VEGETAUX</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fontes, E.P.B. (North Carolina State University, Raleigh, NC)</creatorcontrib><creatorcontrib>Lucknow, V.A</creatorcontrib><creatorcontrib>Hanley-Bowdoin, L</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Plant cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fontes, E.P.B. (North Carolina State University, Raleigh, NC)</au><au>Lucknow, V.A</au><au>Hanley-Bowdoin, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A geminivirus replication protein is a sequence-specific DNA binding protein</atitle><jtitle>The Plant cell</jtitle><addtitle>Plant Cell</addtitle><date>1992-05-01</date><risdate>1992</risdate><volume>4</volume><issue>5</issue><spage>597</spage><epage>608</epage><pages>597-608</pages><issn>1040-4651</issn><eissn>1532-298X</eissn><abstract>The genome of the geminivirus tomato golden mosaic virus (TGMV) consists of two circular DNA molecules designated as components A and B. The A component encodes the only viral protein, AL1, that is required for viral replication. We showed that AL1 interacts specifically with TGMV A and B DNA by using an immunoprecipitation assay for AL1:DNA complex formation. In this assay, a monoclonal antibody against All precipitated AL1:TGMV DNA complexes, whereas an unrelated antibody failed to precipitate the complexes. Competition assays with homologous and heterologous DNAs established the specificity of AL1:DNA binding. All produced by transgenic tobacco plants and by baculovirus-infected insect cells exhibited similar DNA binding activity. The All binding site maps to 52 bp on the left side of the common region, a 235-bp region that is highly conserved between the two TGMV genome components. The AL1:DNA binding site does not include the putative hairpin structure that is conserved in the common regions or the equivalent 5' intergenic regions of all geminiviruses. These studies demonstrate that a geminivirus replication protein is a sequence-specific DNA binding protein, and the studies have important implications for the role of this protein in virus replication</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>1498611</pmid><doi>10.1105/tpc.4.5.597</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Plant cell, 1992-05, Vol.4 (5), p.597-608 |
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| source | MEDLINE; Alma/SFX Local Collection; JSTOR; EZB Electronic Journals Library |
| subjects | ADN Base Sequence Binding Sites Binding, Competitive DNA DNA probes DNA, Viral - metabolism DNA-Binding Proteins - metabolism Electrophoresis, Polyacrylamide Gel Geminiviridae GEMINIVIRUS GENETICA GENETIQUE GENOMAS GENOME Genomes Insect genetics Insect viruses Molecular Sequence Data Mosaic viruses Mosaic Viruses - metabolism Mosaic Viruses - physiology Mosaic Viruses - ultrastructure NUCLEOTIDE NUCLEOTIDOS Plants, Genetically Modified PROTEINAS PROTEINE Transgenic insects Transgenic plants Viral Proteins - metabolism VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX Virus Replication |
| title | A geminivirus replication protein is a sequence-specific DNA binding protein |
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