The rice phytochrome gene: Structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region
We have isolated and characterized both cDNA and genomic clones encoding the apoprotein of rice phytochrome. The mRNA produced from this gene is expressed at a low level in etiolated leaves. Following a flash of red light, the steady-state mRNA level decreases within 15 minutes, and is barely detect...
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| Veröffentlicht in: | The Plant cell 1989-03, Vol.1 (3), p.351-360 |
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| container_title | The Plant cell |
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| creator | Kay, S.A. (The Rockefellar University, New York, NY) Keith, B Shinozaki, K Chye, M.L Chua, N.H |
| description | We have isolated and characterized both cDNA and genomic clones encoding the apoprotein of rice phytochrome. The mRNA produced from this gene is expressed at a low level in etiolated leaves. Following a flash of red light, the steady-state mRNA level decreases within 15 minutes, and is barely detectable after 2 hours. This effect is partially reversed by far red light demonstrating autoregulation of phytochrome mRNA levels. Nuclear run-on experiments show that this effect is exerted on transcription of the phytochrome gene. In etiolated plants, phytochrome mRNA is twofold higher in leaves than in roots, whereas the reverse is true in fully green plants where phytochrome mRNA accumulates despite illumination of the leaves. DNA gel blots and screening of libraries indicate the presence of only a single gene, allowing convenient study of the autoregulatory phenomenon for a specific phytochrome gene. Gel retardation analysis using a fragment from the 5′ upstream region reveals that GT-1 is present in nuclear extracts of etiolated rice leaves and binds to sites conserved between rice and oat phytochrome genes. |
| doi_str_mv | 10.1105/tpc.1.3.351 |
| format | Article |
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(The Rockefellar University, New York, NY) ; Keith, B ; Shinozaki, K ; Chye, M.L ; Chua, N.H</creator><creatorcontrib>Kay, S.A. (The Rockefellar University, New York, NY) ; Keith, B ; Shinozaki, K ; Chye, M.L ; Chua, N.H</creatorcontrib><description>We have isolated and characterized both cDNA and genomic clones encoding the apoprotein of rice phytochrome. The mRNA produced from this gene is expressed at a low level in etiolated leaves. Following a flash of red light, the steady-state mRNA level decreases within 15 minutes, and is barely detectable after 2 hours. This effect is partially reversed by far red light demonstrating autoregulation of phytochrome mRNA levels. Nuclear run-on experiments show that this effect is exerted on transcription of the phytochrome gene. In etiolated plants, phytochrome mRNA is twofold higher in leaves than in roots, whereas the reverse is true in fully green plants where phytochrome mRNA accumulates despite illumination of the leaves. DNA gel blots and screening of libraries indicate the presence of only a single gene, allowing convenient study of the autoregulatory phenomenon for a specific phytochrome gene. Gel retardation analysis using a fragment from the 5′ upstream region reveals that GT-1 is present in nuclear extracts of etiolated rice leaves and binds to sites conserved between rice and oat phytochrome genes.</description><identifier>ISSN: 1040-4651</identifier><identifier>EISSN: 1532-298X</identifier><identifier>DOI: 10.1105/tpc.1.3.351</identifier><identifier>PMID: 2535506</identifier><language>eng</language><publisher>United States: American Society of Plant Physiologists</publisher><subject>ADN ; ANALYTICAL METHODS ; APOPROTEINS ; ARN MENSAJERO ; ARN MESSAGER ; AUTOREGULATION ; Base Sequence ; Binding Sites ; CLONACION ; CLONAGE ; CLONING ; Cloning, Molecular ; Complementary DNA ; DDJB/X14172 ; DNA ; DNA - metabolism ; DNA BINDING PROTEINS ; DNA HYBRIDIZATION ; DNA LIBRARIES ; DNA probes ; DNA-Binding Proteins - metabolism ; EMB1/X14172 ; EXPRESION GENICA ; EXPRESSION DES GENES ; FAR RED LIGHT ; GENBANK/X14172 ; GENE ; GENE EXPRESSION ; Gene Expression Regulation ; GENES ; GENETIC REGULATION ; GENETICA ; GENETICS ; GENETIQUE ; LIGHT ; LIPOPROTEINAS ; LIPOPROTEINE ; LIPOPROTEINS ; LUMIERE ; LUZ ; MESSENGER RNA ; MOLECULAR SEQUENCE DATA ; NORTHERN BLOTTING ; NUCLEAR FACTOR GT-1 ; NUCLEOTIDE ; NUCLEOTIDES ; NUCLEOTIDOS ; Oats ; Oryza - genetics ; ORYZA SATIVA ; Peas ; PHY18 GENE ; Phytochrome - genetics ; PIGMENT ; PIGMENTOS ; PIGMENTS ; Plants ; PROTEINAS ; PROTEINE ; PROTEINS ; RESTRICTION MAPPING ; Rice ; RNA ; SOUTHERN BLOTTING ; TECHNIQUE ANALYTIQUE ; TECNICAS ANALITICAS ; TRANSCRIPTION ; Transcription, Genetic</subject><ispartof>The Plant cell, 1989-03, Vol.1 (3), p.351-360</ispartof><rights>Copyright 1989 American Society of Plant Physiologists</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-6fd669f7fcab69f8b1470053c4652848cc68cc76a523702867cbdf5d7dfc141b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3869014$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3869014$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2535506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kay, S.A. (The Rockefellar University, New York, NY)</creatorcontrib><creatorcontrib>Keith, B</creatorcontrib><creatorcontrib>Shinozaki, K</creatorcontrib><creatorcontrib>Chye, M.L</creatorcontrib><creatorcontrib>Chua, N.H</creatorcontrib><title>The rice phytochrome gene: Structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region</title><title>The Plant cell</title><addtitle>Plant Cell</addtitle><description>We have isolated and characterized both cDNA and genomic clones encoding the apoprotein of rice phytochrome. The mRNA produced from this gene is expressed at a low level in etiolated leaves. Following a flash of red light, the steady-state mRNA level decreases within 15 minutes, and is barely detectable after 2 hours. This effect is partially reversed by far red light demonstrating autoregulation of phytochrome mRNA levels. Nuclear run-on experiments show that this effect is exerted on transcription of the phytochrome gene. In etiolated plants, phytochrome mRNA is twofold higher in leaves than in roots, whereas the reverse is true in fully green plants where phytochrome mRNA accumulates despite illumination of the leaves. DNA gel blots and screening of libraries indicate the presence of only a single gene, allowing convenient study of the autoregulatory phenomenon for a specific phytochrome gene. Gel retardation analysis using a fragment from the 5′ upstream region reveals that GT-1 is present in nuclear extracts of etiolated rice leaves and binds to sites conserved between rice and oat phytochrome genes.</description><subject>ADN</subject><subject>ANALYTICAL METHODS</subject><subject>APOPROTEINS</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>AUTOREGULATION</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>CLONING</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>DDJB/X14172</subject><subject>DNA</subject><subject>DNA - metabolism</subject><subject>DNA BINDING PROTEINS</subject><subject>DNA HYBRIDIZATION</subject><subject>DNA LIBRARIES</subject><subject>DNA probes</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>EMB1/X14172</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>FAR RED LIGHT</subject><subject>GENBANK/X14172</subject><subject>GENE</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Regulation</subject><subject>GENES</subject><subject>GENETIC REGULATION</subject><subject>GENETICA</subject><subject>GENETICS</subject><subject>GENETIQUE</subject><subject>LIGHT</subject><subject>LIPOPROTEINAS</subject><subject>LIPOPROTEINE</subject><subject>LIPOPROTEINS</subject><subject>LUMIERE</subject><subject>LUZ</subject><subject>MESSENGER RNA</subject><subject>MOLECULAR SEQUENCE DATA</subject><subject>NORTHERN BLOTTING</subject><subject>NUCLEAR FACTOR GT-1</subject><subject>NUCLEOTIDE</subject><subject>NUCLEOTIDES</subject><subject>NUCLEOTIDOS</subject><subject>Oats</subject><subject>Oryza - genetics</subject><subject>ORYZA SATIVA</subject><subject>Peas</subject><subject>PHY18 GENE</subject><subject>Phytochrome - genetics</subject><subject>PIGMENT</subject><subject>PIGMENTOS</subject><subject>PIGMENTS</subject><subject>Plants</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>PROTEINS</subject><subject>RESTRICTION MAPPING</subject><subject>Rice</subject><subject>RNA</subject><subject>SOUTHERN BLOTTING</subject><subject>TECHNIQUE ANALYTIQUE</subject><subject>TECNICAS ANALITICAS</subject><subject>TRANSCRIPTION</subject><subject>Transcription, Genetic</subject><issn>1040-4651</issn><issn>1532-298X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkcFrFTEQxhdRals9eROEHEQPus9ks0k2godStAoFD30FbyGbnexL2U22SbbYo_-5kfco9TDMwPebbxK-qnpF8IYQzD7lxWzIhm4oI0-qY8JoUzey-_W0zLjFdcsZeV6dpHSDMSaCyKPqqGGUMcyPqz_bHaDoDKBld5-D2cUwAxrBw2d0leNq8hrhI9JrDhHGddIZBgS_lwgpueCL4gfUOz84P6Jg0cW2JigHpJEJPkG8K3hyGZDzKJdT7D1al5Qj6BkVw2Lxonpm9ZTg5aGfVtffvm7Pv9eXPy9-nJ9d1oY1ONfcDpxLK6zRfeldT1qBMaOmfK_p2s4YXkpwzRoqcNNxYfrBskEM1pCW9PS0-rL3XdZ-hsGAz1FPaolu1vFeBe3U_4p3OzWGO0WYFFyU_XeH_RhuV0hZzS4ZmCbtIaxJCckKJmkBP-xBE0NKEezDDYLVv8BUCUwRRVUJrNBvHj_rgT0kVPS3e_0mlQgeWzUUC0U7LjFpC_Z6j1kdlB6jS-r6ShJMOZX0LzPAp0w</recordid><startdate>19890301</startdate><enddate>19890301</enddate><creator>Kay, S.A. (The Rockefellar University, New York, NY)</creator><creator>Keith, B</creator><creator>Shinozaki, K</creator><creator>Chye, M.L</creator><creator>Chua, N.H</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19890301</creationdate><title>The rice phytochrome gene: Structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region</title><author>Kay, S.A. (The Rockefellar University, New York, NY) ; Keith, B ; Shinozaki, K ; Chye, M.L ; Chua, N.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-6fd669f7fcab69f8b1470053c4652848cc68cc76a523702867cbdf5d7dfc141b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>ADN</topic><topic>ANALYTICAL METHODS</topic><topic>APOPROTEINS</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>AUTOREGULATION</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>CLONING</topic><topic>Cloning, Molecular</topic><topic>Complementary DNA</topic><topic>DDJB/X14172</topic><topic>DNA</topic><topic>DNA - metabolism</topic><topic>DNA BINDING PROTEINS</topic><topic>DNA HYBRIDIZATION</topic><topic>DNA LIBRARIES</topic><topic>DNA probes</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>EMB1/X14172</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>FAR RED LIGHT</topic><topic>GENBANK/X14172</topic><topic>GENE</topic><topic>GENE EXPRESSION</topic><topic>Gene Expression Regulation</topic><topic>GENES</topic><topic>GENETIC REGULATION</topic><topic>GENETICA</topic><topic>GENETICS</topic><topic>GENETIQUE</topic><topic>LIGHT</topic><topic>LIPOPROTEINAS</topic><topic>LIPOPROTEINE</topic><topic>LIPOPROTEINS</topic><topic>LUMIERE</topic><topic>LUZ</topic><topic>MESSENGER RNA</topic><topic>MOLECULAR SEQUENCE DATA</topic><topic>NORTHERN BLOTTING</topic><topic>NUCLEAR FACTOR GT-1</topic><topic>NUCLEOTIDE</topic><topic>NUCLEOTIDES</topic><topic>NUCLEOTIDOS</topic><topic>Oats</topic><topic>Oryza - genetics</topic><topic>ORYZA SATIVA</topic><topic>Peas</topic><topic>PHY18 GENE</topic><topic>Phytochrome - genetics</topic><topic>PIGMENT</topic><topic>PIGMENTOS</topic><topic>PIGMENTS</topic><topic>Plants</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>PROTEINS</topic><topic>RESTRICTION MAPPING</topic><topic>Rice</topic><topic>RNA</topic><topic>SOUTHERN BLOTTING</topic><topic>TECHNIQUE ANALYTIQUE</topic><topic>TECNICAS ANALITICAS</topic><topic>TRANSCRIPTION</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kay, S.A. (The Rockefellar University, New York, NY)</creatorcontrib><creatorcontrib>Keith, B</creatorcontrib><creatorcontrib>Shinozaki, K</creatorcontrib><creatorcontrib>Chye, M.L</creatorcontrib><creatorcontrib>Chua, N.H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>The Plant cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kay, S.A. (The Rockefellar University, New York, NY)</au><au>Keith, B</au><au>Shinozaki, K</au><au>Chye, M.L</au><au>Chua, N.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The rice phytochrome gene: Structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region</atitle><jtitle>The Plant cell</jtitle><addtitle>Plant Cell</addtitle><date>1989-03-01</date><risdate>1989</risdate><volume>1</volume><issue>3</issue><spage>351</spage><epage>360</epage><pages>351-360</pages><issn>1040-4651</issn><eissn>1532-298X</eissn><abstract>We have isolated and characterized both cDNA and genomic clones encoding the apoprotein of rice phytochrome. The mRNA produced from this gene is expressed at a low level in etiolated leaves. Following a flash of red light, the steady-state mRNA level decreases within 15 minutes, and is barely detectable after 2 hours. This effect is partially reversed by far red light demonstrating autoregulation of phytochrome mRNA levels. Nuclear run-on experiments show that this effect is exerted on transcription of the phytochrome gene. In etiolated plants, phytochrome mRNA is twofold higher in leaves than in roots, whereas the reverse is true in fully green plants where phytochrome mRNA accumulates despite illumination of the leaves. DNA gel blots and screening of libraries indicate the presence of only a single gene, allowing convenient study of the autoregulatory phenomenon for a specific phytochrome gene. Gel retardation analysis using a fragment from the 5′ upstream region reveals that GT-1 is present in nuclear extracts of etiolated rice leaves and binds to sites conserved between rice and oat phytochrome genes.</abstract><cop>United States</cop><pub>American Society of Plant Physiologists</pub><pmid>2535506</pmid><doi>10.1105/tpc.1.3.351</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1040-4651 |
| ispartof | The Plant cell, 1989-03, Vol.1 (3), p.351-360 |
| issn | 1040-4651 1532-298X |
| language | eng |
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| source | Jstor Complete Legacy; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | ADN ANALYTICAL METHODS APOPROTEINS ARN MENSAJERO ARN MESSAGER AUTOREGULATION Base Sequence Binding Sites CLONACION CLONAGE CLONING Cloning, Molecular Complementary DNA DDJB/X14172 DNA DNA - metabolism DNA BINDING PROTEINS DNA HYBRIDIZATION DNA LIBRARIES DNA probes DNA-Binding Proteins - metabolism EMB1/X14172 EXPRESION GENICA EXPRESSION DES GENES FAR RED LIGHT GENBANK/X14172 GENE GENE EXPRESSION Gene Expression Regulation GENES GENETIC REGULATION GENETICA GENETICS GENETIQUE LIGHT LIPOPROTEINAS LIPOPROTEINE LIPOPROTEINS LUMIERE LUZ MESSENGER RNA MOLECULAR SEQUENCE DATA NORTHERN BLOTTING NUCLEAR FACTOR GT-1 NUCLEOTIDE NUCLEOTIDES NUCLEOTIDOS Oats Oryza - genetics ORYZA SATIVA Peas PHY18 GENE Phytochrome - genetics PIGMENT PIGMENTOS PIGMENTS Plants PROTEINAS PROTEINE PROTEINS RESTRICTION MAPPING Rice RNA SOUTHERN BLOTTING TECHNIQUE ANALYTIQUE TECNICAS ANALITICAS TRANSCRIPTION Transcription, Genetic |
| title | The rice phytochrome gene: Structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region |
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