The use of fura-2 fluorescence to monitor the movement of free calcium ions into the matrix of plant mitochondria (Pisum sativum and Helianthus tuberosus)

Purified mitochondria isolated from pea (Pisum sativum L. cv Alaska) stems and Jerusalem artichoke Helianthus tuberosus L. cv OB1) tubers were loaded with the acetoxymethyl ester of the fluorescent Ca2+ indicator fura-2. This made possible the continuous monitoring of free [Ca2+] in the matrix ([Ca2...

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Veröffentlicht in:	Plant physiology (Bethesda) 1993-06, Vol.102 (2), p.573-578
Hauptverfasser:	Zottini, M, Zannoni, D
Format:	Artikel
Sprache:	eng
Schlagworte:	ANION ANIONES Artichokes Bioenergetics Biological and medical sciences CALCIO CALCIUM CATION CATIONES Cell biochemistry Cell physiology Cell structures and functions Dehydrogenases Enzymes Fluorescence FOSFORO Fundamental and applied biological sciences. Psychology HELIANTHUS TUBEROSUS ION IONES MEMBRANA MEMBRANE Mitochondria Mitochondria and cell respiration MITOCHONDRIE MITOCONDRIA Molecular and cellular biology Peas PHOSPHORE PISUM SATIVUM Plant physiology and development Plants Stems TALLO TECHNIQUE DES TRACEURS TECNICAS DE TRAZADORES TIGE TUBERCULE TUBERCULO Tubers
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