Differential effect of purified spruce chitinases and beta-1,3-glucanases on the activity of elicitors from ectomycorrhizal fungi
Two chitinases (EC 3.2.1.14) and two beta-1,3-glucanases (EC 3.2.1.39) were purified from the culture medium of spruce (Picea abies [L.] Karst.) cells to study their role in modifying elicitors, cell walls, growth, and hyphal morphology of ectomycorrhizal fungi. The 36-kD class I chitinase (isoelect...
Gespeichert in:
| Veröffentlicht in: | Plant physiology (Bethesda) 1997-07, Vol.114 (3), p.957-968 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 968 |
|---|---|
| container_issue | 3 |
| container_start_page | 957 |
| container_title | Plant physiology (Bethesda) |
| container_volume | 114 |
| creator | Salzer, P Hubner, B Sirrenberg, A Hager, A |
| description | Two chitinases (EC 3.2.1.14) and two beta-1,3-glucanases (EC 3.2.1.39) were purified from the culture medium of spruce (Picea abies [L.] Karst.) cells to study their role in modifying elicitors, cell walls, growth, and hyphal morphology of ectomycorrhizal fungi. The 36-kD class I chitinase (isoelectric point [pI] 8.0) and the 28-kD chitinase (pI 8.7) decreased the activity of elicitor preparations from Hebeloma crustuliniforme (Bull. ex Fries.) Quell, Amanita muscaria (L.) Pers., and Suillus variegatus (Sw.: Fr.) O.K., as demonstrated by using the elicitor-induced extracellular alkalinization in spruce cells as a test system. In addition, chitinases released monomeric products from the walls of these ectomycorrhizal fungi. The beta-1,3-glucanases (35 kD, pI 3.7 and 3.9), in contrast, had little influence on the activity of the fungal elicitors and released only from walls of A. muscaria some polymeric products. Furthermore, chitinases alone and in combination with beta-1,3-glucanases had no effect on the growth and morphology of the hyphae. Thus, it is suggested that apoplastic chitinases in the root cortex destroy elicitors from the ectomycorrhizal fungi without damaging the fungus. By this mechanism the host plant could attenuate the elicitor signal and adjust its own defense reactions to a level allowing symbiotic interaction |
| doi_str_mv | 10.1104/pp.114.3.957 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_158384</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4277789</jstor_id><sourcerecordid>4277789</sourcerecordid><originalsourceid>FETCH-LOGICAL-c512t-7f35bbafae8d139f5e6fe14a6d5fe510348fe5f04f3e4091e446f9941ffdf0753</originalsourceid><addsrcrecordid>eNqFUj1vFDEQXSFQuAQ6SpBcoFTZw17ba7tIgcKnFIkCUls-78ydo731Yu9GOjr-OT7d6YAq1Rv5vXkz45mqesXokjEq3o1jQbHkSyPVk2rBJG_qRgr9tFpQWmKqtXlened8TyllnImz6sw0vNFKLarfHwIiJBim4HoCJfYTiUjGOQUM0JE8ptkD8ZswhcFlyMQNHVnB5Gp2xet1P3t3eI8DmTZAnJ_CQ5h2exfogw9TTJlgiltSvON252NKm_CrlMN5WIcX1TN0fYaXR7yo7j59_HHzpb799vnrzfvb2kvWTLVCLlcrhw50x7hBCS0CE67tJIJklAtdEKlADoIaBkK0aIxgiB1SJflFdX3wHefVFjpfRk6ut2MKW5d2Nrpg_2eGsLHr-GCZ1FyLkn95zE_x5wx5stuQPfS9GyDO2SrDZFs-9VGh0EK1QrNHhazlZb-iKcKrg9CnmHMCPHXNqN3fgB3HgsJyW26gyN_8O-lJfFx64d8eeZe96zG5wYd8kjVKGKX27b0-yO5zWeGJFo1SSpu_VdBF69apONx9Z8Yo2pYja_kfwYjPTQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16310442</pqid></control><display><type>article</type><title>Differential effect of purified spruce chitinases and beta-1,3-glucanases on the activity of elicitors from ectomycorrhizal fungi</title><source>Jstor Complete Legacy</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Salzer, P ; Hubner, B ; Sirrenberg, A ; Hager, A</creator><creatorcontrib>Salzer, P ; Hubner, B ; Sirrenberg, A ; Hager, A</creatorcontrib><description>Two chitinases (EC 3.2.1.14) and two beta-1,3-glucanases (EC 3.2.1.39) were purified from the culture medium of spruce (Picea abies [L.] Karst.) cells to study their role in modifying elicitors, cell walls, growth, and hyphal morphology of ectomycorrhizal fungi. The 36-kD class I chitinase (isoelectric point [pI] 8.0) and the 28-kD chitinase (pI 8.7) decreased the activity of elicitor preparations from Hebeloma crustuliniforme (Bull. ex Fries.) Quell, Amanita muscaria (L.) Pers., and Suillus variegatus (Sw.: Fr.) O.K., as demonstrated by using the elicitor-induced extracellular alkalinization in spruce cells as a test system. In addition, chitinases released monomeric products from the walls of these ectomycorrhizal fungi. The beta-1,3-glucanases (35 kD, pI 3.7 and 3.9), in contrast, had little influence on the activity of the fungal elicitors and released only from walls of A. muscaria some polymeric products. Furthermore, chitinases alone and in combination with beta-1,3-glucanases had no effect on the growth and morphology of the hyphae. Thus, it is suggested that apoplastic chitinases in the root cortex destroy elicitors from the ectomycorrhizal fungi without damaging the fungus. By this mechanism the host plant could attenuate the elicitor signal and adjust its own defense reactions to a level allowing symbiotic interaction</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.114.3.957</identifier><identifier>PMID: 9232877</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Agronomy. Soil science and plant productions ; AMANITA ; Amanita - physiology ; AMANITA MUSCARIA ; Amino Acid Sequence ; Basidiomycota ; Basidiomycota - physiology ; BETA GLUCANASA ; BETA GLUCANASE ; beta-Glucosidase ; beta-Glucosidase - isolation & purification ; beta-Glucosidase - metabolism ; Biological and medical sciences ; biosynthesis ; CELL CULTURE ; cell growth ; cell wall components ; CELL WALLS ; CELLS ; Cells, Cultured ; CELLULE ; CELULAS ; chemistry ; Chitin ; Chitin - isolation & purification ; Chitin - metabolism ; CHITINASE ; Chitinases ; Chitinases - chemistry ; Chitinases - isolation & purification ; Chitinases - metabolism ; Chromatography ; Chromatography, Affinity ; Chromatography, Ion Exchange ; CRECIMIENTO ; CROISSANCE ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; Cultured cells ; DEFENCE MECHANISMS ; defense mechanisms ; Economic plant physiology ; ectomycorrhizae ; enzyme activity ; Enzymes ; ENZYMIC ACTIVITY ; enzymology ; Fundamental and applied biological sciences. Psychology ; FUNGAL MORPHOLOGY ; Fungal Proteins ; Fungal Proteins - metabolism ; Fungi ; Glucan 1,3-beta-Glucosidase ; GROWTH ; HEBELOMA ; HEBELOMA CRUSTULINIFORME ; HYPHAE ; INFECTIVITY ; INHIBICION ; INHIBITION ; Isoenzymes ; Isoenzymes - biosynthesis ; Isoenzymes - chemistry ; Isoenzymes - metabolism ; isolation & purification ; MECANISME DE DEFENSE ; MECANISMOS DE DEFENSA ; metabolism ; MICELIO ; microbiology ; Molecular Sequence Data ; Molecular Weight ; MORFOLOGIA FUNGICA ; MORPHOLOGIE DE CHAMPIGNON ; MYCELIUM ; MYCORHIZE ; MYCORRHIZAE ; Parasitism and symbiosis ; PARED CELULAR ; PAROI CELLULAIRE ; PATHOGENICITY ; PATOGENICIDAD ; physiology ; PICEA ABIES ; Plant physiology and development ; Plant roots ; Plant-Microbe and Plant-Insect Interactions ; Plants ; POUVOIR PATHOGENE ; Protein isoforms ; Proteins ; PURIFICACION ; PURIFICATION ; QUITINASA ; Sequence Alignment ; Sequence Homology, Amino Acid ; SIMBIOSIS ; SUILLUS ; SYMBIOSE ; SYMBIOSIS ; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) ; Trees ; Trees - enzymology ; Trees - microbiology</subject><ispartof>Plant physiology (Bethesda), 1997-07, Vol.114 (3), p.957-968</ispartof><rights>Copyright 1997 American Society of Plant Physiologists</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-7f35bbafae8d139f5e6fe14a6d5fe510348fe5f04f3e4091e446f9941ffdf0753</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4277789$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4277789$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2749771$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9232877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Salzer, P</creatorcontrib><creatorcontrib>Hubner, B</creatorcontrib><creatorcontrib>Sirrenberg, A</creatorcontrib><creatorcontrib>Hager, A</creatorcontrib><title>Differential effect of purified spruce chitinases and beta-1,3-glucanases on the activity of elicitors from ectomycorrhizal fungi</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Two chitinases (EC 3.2.1.14) and two beta-1,3-glucanases (EC 3.2.1.39) were purified from the culture medium of spruce (Picea abies [L.] Karst.) cells to study their role in modifying elicitors, cell walls, growth, and hyphal morphology of ectomycorrhizal fungi. The 36-kD class I chitinase (isoelectric point [pI] 8.0) and the 28-kD chitinase (pI 8.7) decreased the activity of elicitor preparations from Hebeloma crustuliniforme (Bull. ex Fries.) Quell, Amanita muscaria (L.) Pers., and Suillus variegatus (Sw.: Fr.) O.K., as demonstrated by using the elicitor-induced extracellular alkalinization in spruce cells as a test system. In addition, chitinases released monomeric products from the walls of these ectomycorrhizal fungi. The beta-1,3-glucanases (35 kD, pI 3.7 and 3.9), in contrast, had little influence on the activity of the fungal elicitors and released only from walls of A. muscaria some polymeric products. Furthermore, chitinases alone and in combination with beta-1,3-glucanases had no effect on the growth and morphology of the hyphae. Thus, it is suggested that apoplastic chitinases in the root cortex destroy elicitors from the ectomycorrhizal fungi without damaging the fungus. By this mechanism the host plant could attenuate the elicitor signal and adjust its own defense reactions to a level allowing symbiotic interaction</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>Agronomy. Soil science and plant productions</subject><subject>AMANITA</subject><subject>Amanita - physiology</subject><subject>AMANITA MUSCARIA</subject><subject>Amino Acid Sequence</subject><subject>Basidiomycota</subject><subject>Basidiomycota - physiology</subject><subject>BETA GLUCANASA</subject><subject>BETA GLUCANASE</subject><subject>beta-Glucosidase</subject><subject>beta-Glucosidase - isolation & purification</subject><subject>beta-Glucosidase - metabolism</subject><subject>Biological and medical sciences</subject><subject>biosynthesis</subject><subject>CELL CULTURE</subject><subject>cell growth</subject><subject>cell wall components</subject><subject>CELL WALLS</subject><subject>CELLS</subject><subject>Cells, Cultured</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>chemistry</subject><subject>Chitin</subject><subject>Chitin - isolation & purification</subject><subject>Chitin - metabolism</subject><subject>CHITINASE</subject><subject>Chitinases</subject><subject>Chitinases - chemistry</subject><subject>Chitinases - isolation & purification</subject><subject>Chitinases - metabolism</subject><subject>Chromatography</subject><subject>Chromatography, Affinity</subject><subject>Chromatography, Ion Exchange</subject><subject>CRECIMIENTO</subject><subject>CROISSANCE</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>Cultured cells</subject><subject>DEFENCE MECHANISMS</subject><subject>defense mechanisms</subject><subject>Economic plant physiology</subject><subject>ectomycorrhizae</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>ENZYMIC ACTIVITY</subject><subject>enzymology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>FUNGAL MORPHOLOGY</subject><subject>Fungal Proteins</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungi</subject><subject>Glucan 1,3-beta-Glucosidase</subject><subject>GROWTH</subject><subject>HEBELOMA</subject><subject>HEBELOMA CRUSTULINIFORME</subject><subject>HYPHAE</subject><subject>INFECTIVITY</subject><subject>INHIBICION</subject><subject>INHIBITION</subject><subject>Isoenzymes</subject><subject>Isoenzymes - biosynthesis</subject><subject>Isoenzymes - chemistry</subject><subject>Isoenzymes - metabolism</subject><subject>isolation & purification</subject><subject>MECANISME DE DEFENSE</subject><subject>MECANISMOS DE DEFENSA</subject><subject>metabolism</subject><subject>MICELIO</subject><subject>microbiology</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>MORFOLOGIA FUNGICA</subject><subject>MORPHOLOGIE DE CHAMPIGNON</subject><subject>MYCELIUM</subject><subject>MYCORHIZE</subject><subject>MYCORRHIZAE</subject><subject>Parasitism and symbiosis</subject><subject>PARED CELULAR</subject><subject>PAROI CELLULAIRE</subject><subject>PATHOGENICITY</subject><subject>PATOGENICIDAD</subject><subject>physiology</subject><subject>PICEA ABIES</subject><subject>Plant physiology and development</subject><subject>Plant roots</subject><subject>Plant-Microbe and Plant-Insect Interactions</subject><subject>Plants</subject><subject>POUVOIR PATHOGENE</subject><subject>Protein isoforms</subject><subject>Proteins</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>QUITINASA</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>SIMBIOSIS</subject><subject>SUILLUS</subject><subject>SYMBIOSE</subject><subject>SYMBIOSIS</subject><subject>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</subject><subject>Trees</subject><subject>Trees - enzymology</subject><subject>Trees - microbiology</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUj1vFDEQXSFQuAQ6SpBcoFTZw17ba7tIgcKnFIkCUls-78ydo731Yu9GOjr-OT7d6YAq1Rv5vXkz45mqesXokjEq3o1jQbHkSyPVk2rBJG_qRgr9tFpQWmKqtXlened8TyllnImz6sw0vNFKLarfHwIiJBim4HoCJfYTiUjGOQUM0JE8ptkD8ZswhcFlyMQNHVnB5Gp2xet1P3t3eI8DmTZAnJ_CQ5h2exfogw9TTJlgiltSvON252NKm_CrlMN5WIcX1TN0fYaXR7yo7j59_HHzpb799vnrzfvb2kvWTLVCLlcrhw50x7hBCS0CE67tJIJklAtdEKlADoIaBkK0aIxgiB1SJflFdX3wHefVFjpfRk6ut2MKW5d2Nrpg_2eGsLHr-GCZ1FyLkn95zE_x5wx5stuQPfS9GyDO2SrDZFs-9VGh0EK1QrNHhazlZb-iKcKrg9CnmHMCPHXNqN3fgB3HgsJyW26gyN_8O-lJfFx64d8eeZe96zG5wYd8kjVKGKX27b0-yO5zWeGJFo1SSpu_VdBF69apONx9Z8Yo2pYja_kfwYjPTQ</recordid><startdate>19970701</startdate><enddate>19970701</enddate><creator>Salzer, P</creator><creator>Hubner, B</creator><creator>Sirrenberg, A</creator><creator>Hager, A</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970701</creationdate><title>Differential effect of purified spruce chitinases and beta-1,3-glucanases on the activity of elicitors from ectomycorrhizal fungi</title><author>Salzer, P ; Hubner, B ; Sirrenberg, A ; Hager, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-7f35bbafae8d139f5e6fe14a6d5fe510348fe5f04f3e4091e446f9941ffdf0753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>Agronomy. Soil science and plant productions</topic><topic>AMANITA</topic><topic>Amanita - physiology</topic><topic>AMANITA MUSCARIA</topic><topic>Amino Acid Sequence</topic><topic>Basidiomycota</topic><topic>Basidiomycota - physiology</topic><topic>BETA GLUCANASA</topic><topic>BETA GLUCANASE</topic><topic>beta-Glucosidase</topic><topic>beta-Glucosidase - isolation & purification</topic><topic>beta-Glucosidase - metabolism</topic><topic>Biological and medical sciences</topic><topic>biosynthesis</topic><topic>CELL CULTURE</topic><topic>cell growth</topic><topic>cell wall components</topic><topic>CELL WALLS</topic><topic>CELLS</topic><topic>Cells, Cultured</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>chemistry</topic><topic>Chitin</topic><topic>Chitin - isolation & purification</topic><topic>Chitin - metabolism</topic><topic>CHITINASE</topic><topic>Chitinases</topic><topic>Chitinases - chemistry</topic><topic>Chitinases - isolation & purification</topic><topic>Chitinases - metabolism</topic><topic>Chromatography</topic><topic>Chromatography, Affinity</topic><topic>Chromatography, Ion Exchange</topic><topic>CRECIMIENTO</topic><topic>CROISSANCE</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>Cultured cells</topic><topic>DEFENCE MECHANISMS</topic><topic>defense mechanisms</topic><topic>Economic plant physiology</topic><topic>ectomycorrhizae</topic><topic>enzyme activity</topic><topic>Enzymes</topic><topic>ENZYMIC ACTIVITY</topic><topic>enzymology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>FUNGAL MORPHOLOGY</topic><topic>Fungal Proteins</topic><topic>Fungal Proteins - metabolism</topic><topic>Fungi</topic><topic>Glucan 1,3-beta-Glucosidase</topic><topic>GROWTH</topic><topic>HEBELOMA</topic><topic>HEBELOMA CRUSTULINIFORME</topic><topic>HYPHAE</topic><topic>INFECTIVITY</topic><topic>INHIBICION</topic><topic>INHIBITION</topic><topic>Isoenzymes</topic><topic>Isoenzymes - biosynthesis</topic><topic>Isoenzymes - chemistry</topic><topic>Isoenzymes - metabolism</topic><topic>isolation & purification</topic><topic>MECANISME DE DEFENSE</topic><topic>MECANISMOS DE DEFENSA</topic><topic>metabolism</topic><topic>MICELIO</topic><topic>microbiology</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>MORFOLOGIA FUNGICA</topic><topic>MORPHOLOGIE DE CHAMPIGNON</topic><topic>MYCELIUM</topic><topic>MYCORHIZE</topic><topic>MYCORRHIZAE</topic><topic>Parasitism and symbiosis</topic><topic>PARED CELULAR</topic><topic>PAROI CELLULAIRE</topic><topic>PATHOGENICITY</topic><topic>PATOGENICIDAD</topic><topic>physiology</topic><topic>PICEA ABIES</topic><topic>Plant physiology and development</topic><topic>Plant roots</topic><topic>Plant-Microbe and Plant-Insect Interactions</topic><topic>Plants</topic><topic>POUVOIR PATHOGENE</topic><topic>Protein isoforms</topic><topic>Proteins</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>QUITINASA</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>SIMBIOSIS</topic><topic>SUILLUS</topic><topic>SYMBIOSE</topic><topic>SYMBIOSIS</topic><topic>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</topic><topic>Trees</topic><topic>Trees - enzymology</topic><topic>Trees - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Salzer, P</creatorcontrib><creatorcontrib>Hubner, B</creatorcontrib><creatorcontrib>Sirrenberg, A</creatorcontrib><creatorcontrib>Hager, A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Salzer, P</au><au>Hubner, B</au><au>Sirrenberg, A</au><au>Hager, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential effect of purified spruce chitinases and beta-1,3-glucanases on the activity of elicitors from ectomycorrhizal fungi</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1997-07-01</date><risdate>1997</risdate><volume>114</volume><issue>3</issue><spage>957</spage><epage>968</epage><pages>957-968</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Two chitinases (EC 3.2.1.14) and two beta-1,3-glucanases (EC 3.2.1.39) were purified from the culture medium of spruce (Picea abies [L.] Karst.) cells to study their role in modifying elicitors, cell walls, growth, and hyphal morphology of ectomycorrhizal fungi. The 36-kD class I chitinase (isoelectric point [pI] 8.0) and the 28-kD chitinase (pI 8.7) decreased the activity of elicitor preparations from Hebeloma crustuliniforme (Bull. ex Fries.) Quell, Amanita muscaria (L.) Pers., and Suillus variegatus (Sw.: Fr.) O.K., as demonstrated by using the elicitor-induced extracellular alkalinization in spruce cells as a test system. In addition, chitinases released monomeric products from the walls of these ectomycorrhizal fungi. The beta-1,3-glucanases (35 kD, pI 3.7 and 3.9), in contrast, had little influence on the activity of the fungal elicitors and released only from walls of A. muscaria some polymeric products. Furthermore, chitinases alone and in combination with beta-1,3-glucanases had no effect on the growth and morphology of the hyphae. Thus, it is suggested that apoplastic chitinases in the root cortex destroy elicitors from the ectomycorrhizal fungi without damaging the fungus. By this mechanism the host plant could attenuate the elicitor signal and adjust its own defense reactions to a level allowing symbiotic interaction</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>9232877</pmid><doi>10.1104/pp.114.3.957</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1997-07, Vol.114 (3), p.957-968 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_158384 |
| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Agronomy. Soil science and plant productions AMANITA Amanita - physiology AMANITA MUSCARIA Amino Acid Sequence Basidiomycota Basidiomycota - physiology BETA GLUCANASA BETA GLUCANASE beta-Glucosidase beta-Glucosidase - isolation & purification beta-Glucosidase - metabolism Biological and medical sciences biosynthesis CELL CULTURE cell growth cell wall components CELL WALLS CELLS Cells, Cultured CELLULE CELULAS chemistry Chitin Chitin - isolation & purification Chitin - metabolism CHITINASE Chitinases Chitinases - chemistry Chitinases - isolation & purification Chitinases - metabolism Chromatography Chromatography, Affinity Chromatography, Ion Exchange CRECIMIENTO CROISSANCE CULTIVO DE CELULAS CULTURE DE CELLULE Cultured cells DEFENCE MECHANISMS defense mechanisms Economic plant physiology ectomycorrhizae enzyme activity Enzymes ENZYMIC ACTIVITY enzymology Fundamental and applied biological sciences. Psychology FUNGAL MORPHOLOGY Fungal Proteins Fungal Proteins - metabolism Fungi Glucan 1,3-beta-Glucosidase GROWTH HEBELOMA HEBELOMA CRUSTULINIFORME HYPHAE INFECTIVITY INHIBICION INHIBITION Isoenzymes Isoenzymes - biosynthesis Isoenzymes - chemistry Isoenzymes - metabolism isolation & purification MECANISME DE DEFENSE MECANISMOS DE DEFENSA metabolism MICELIO microbiology Molecular Sequence Data Molecular Weight MORFOLOGIA FUNGICA MORPHOLOGIE DE CHAMPIGNON MYCELIUM MYCORHIZE MYCORRHIZAE Parasitism and symbiosis PARED CELULAR PAROI CELLULAIRE PATHOGENICITY PATOGENICIDAD physiology PICEA ABIES Plant physiology and development Plant roots Plant-Microbe and Plant-Insect Interactions Plants POUVOIR PATHOGENE Protein isoforms Proteins PURIFICACION PURIFICATION QUITINASA Sequence Alignment Sequence Homology, Amino Acid SIMBIOSIS SUILLUS SYMBIOSE SYMBIOSIS Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) Trees Trees - enzymology Trees - microbiology |
| title | Differential effect of purified spruce chitinases and beta-1,3-glucanases on the activity of elicitors from ectomycorrhizal fungi |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T00%3A39%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differential%20effect%20of%20purified%20spruce%20chitinases%20and%20beta-1,3-glucanases%20on%20the%20activity%20of%20elicitors%20from%20ectomycorrhizal%20fungi&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Salzer,%20P&rft.date=1997-07-01&rft.volume=114&rft.issue=3&rft.spage=957&rft.epage=968&rft.pages=957-968&rft.issn=0032-0889&rft.eissn=1532-2548&rft.coden=PPHYA5&rft_id=info:doi/10.1104/pp.114.3.957&rft_dat=%3Cjstor_pubme%3E4277789%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16310442&rft_id=info:pmid/9232877&rft_jstor_id=4277789&rfr_iscdi=true |