Characterization of Flavonol Synthase and Leucoanthocyanidin Dioxygenase Genes in Arabidopsis: Further Evidence for Differential Regulation of "Early" and "Late" Genes
As part of an ongoing investigation into the organization and regulation of the flavonoid biosynthetic pathway, two Arabidopsis thaliana expressed sequence tag (EST) clones (153O10T7 and YAY780) with high homology to leucoanthocyanidin dioxygenase (LDOX) or flavonol synthase (FLS) were identified. E...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1997-04, Vol.113 (4), p.1437-1445 |
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| creator | Matthew K. Pelletier Murrell, Julie R. Shirley, Brenda W. |
| description | As part of an ongoing investigation into the organization and regulation of the flavonoid biosynthetic pathway, two Arabidopsis thaliana expressed sequence tag (EST) clones (153O10T7 and YAY780) with high homology to leucoanthocyanidin dioxygenase (LDOX) or flavonol synthase (FLS) were identified. EST YAY780 was sequenced and found to encode a protein 49 to 78% identical to all LDOX sequences in the database. EST 153O10T7 was used to isolate a genomic clone encoding a protein with 59 to 61% sequence identity to petunia (Petunia hybrida) and potato (Solanum tuberosum) FLS. DNA blot analysis was used to screen the Arabidopsis genome for sequences related to FLS and LDOX and to determine the positions of the two clones on the RI map. The expression patterns of FLS and LDOX in etiolated seedlings moved to white light and in two putative regulatory mutants (ttg and tt8) were determined by RNA blot analysis. These studies indicate that FLS is an "early" flavonoid gene in Arabidopsis seedlings, whereas LDOX is a "late" gene. Furthermore, FLS is the first flavonoid enzyme identified in Arabidopsis that may be encoded by a gene family. |
| doi_str_mv | 10.1104/pp.113.4.1437 |
| format | Article |
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Pelletier ; Murrell, Julie R. ; Shirley, Brenda W.</creator><creatorcontrib>Matthew K. Pelletier ; Murrell, Julie R. ; Shirley, Brenda W.</creatorcontrib><description>As part of an ongoing investigation into the organization and regulation of the flavonoid biosynthetic pathway, two Arabidopsis thaliana expressed sequence tag (EST) clones (153O10T7 and YAY780) with high homology to leucoanthocyanidin dioxygenase (LDOX) or flavonol synthase (FLS) were identified. EST YAY780 was sequenced and found to encode a protein 49 to 78% identical to all LDOX sequences in the database. EST 153O10T7 was used to isolate a genomic clone encoding a protein with 59 to 61% sequence identity to petunia (Petunia hybrida) and potato (Solanum tuberosum) FLS. DNA blot analysis was used to screen the Arabidopsis genome for sequences related to FLS and LDOX and to determine the positions of the two clones on the RI map. The expression patterns of FLS and LDOX in etiolated seedlings moved to white light and in two putative regulatory mutants (ttg and tt8) were determined by RNA blot analysis. These studies indicate that FLS is an "early" flavonoid gene in Arabidopsis seedlings, whereas LDOX is a "late" gene. Furthermore, FLS is the first flavonoid enzyme identified in Arabidopsis that may be encoded by a gene family.</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.113.4.1437</identifier><identifier>PMID: 9112784</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Amino Acid Sequence ; Amino acids ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Biological and medical sciences ; Cloning, Molecular ; Darkness ; DNA ; Enzymes ; Flavonoids ; Flavonols ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Gene Regulation and Molecular Genetics ; Genes, Plant ; Genes. Genome ; Genomics ; Information Systems ; Light ; Messenger RNA ; Metabolism ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Multigene Family ; Oxidoreductases - biosynthesis ; Oxidoreductases - chemistry ; Oxidoreductases - genetics ; Oxygenases - biosynthesis ; Oxygenases - chemistry ; Oxygenases - genetics ; Plant physiology and development ; Plant Proteins ; Plants ; Plants - enzymology ; RNA ; Seedlings ; Sequence Homology, Amino Acid ; Sequence Tagged Sites ; Solanum tuberosum - enzymology ; Storage and secretion, pigments, phytochrome</subject><ispartof>Plant physiology (Bethesda), 1997-04, Vol.113 (4), p.1437-1445</ispartof><rights>Copyright 1997 American Society of Plant Physiologists</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4277667$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4277667$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2693686$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9112784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matthew K. Pelletier</creatorcontrib><creatorcontrib>Murrell, Julie R.</creatorcontrib><creatorcontrib>Shirley, Brenda W.</creatorcontrib><title>Characterization of Flavonol Synthase and Leucoanthocyanidin Dioxygenase Genes in Arabidopsis: Further Evidence for Differential Regulation of "Early" and "Late" Genes</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>As part of an ongoing investigation into the organization and regulation of the flavonoid biosynthetic pathway, two Arabidopsis thaliana expressed sequence tag (EST) clones (153O10T7 and YAY780) with high homology to leucoanthocyanidin dioxygenase (LDOX) or flavonol synthase (FLS) were identified. EST YAY780 was sequenced and found to encode a protein 49 to 78% identical to all LDOX sequences in the database. EST 153O10T7 was used to isolate a genomic clone encoding a protein with 59 to 61% sequence identity to petunia (Petunia hybrida) and potato (Solanum tuberosum) FLS. DNA blot analysis was used to screen the Arabidopsis genome for sequences related to FLS and LDOX and to determine the positions of the two clones on the RI map. The expression patterns of FLS and LDOX in etiolated seedlings moved to white light and in two putative regulatory mutants (ttg and tt8) were determined by RNA blot analysis. These studies indicate that FLS is an "early" flavonoid gene in Arabidopsis seedlings, whereas LDOX is a "late" gene. Furthermore, FLS is the first flavonoid enzyme identified in Arabidopsis that may be encoded by a gene family.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Darkness</subject><subject>DNA</subject><subject>Enzymes</subject><subject>Flavonoids</subject><subject>Flavonols</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Regulation and Molecular Genetics</subject><subject>Genes, Plant</subject><subject>Genes. Genome</subject><subject>Genomics</subject><subject>Information Systems</subject><subject>Light</subject><subject>Messenger RNA</subject><subject>Metabolism</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Oxidoreductases - biosynthesis</subject><subject>Oxidoreductases - chemistry</subject><subject>Oxidoreductases - genetics</subject><subject>Oxygenases - biosynthesis</subject><subject>Oxygenases - chemistry</subject><subject>Oxygenases - genetics</subject><subject>Plant physiology and development</subject><subject>Plant Proteins</subject><subject>Plants</subject><subject>Plants - enzymology</subject><subject>RNA</subject><subject>Seedlings</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Tagged Sites</subject><subject>Solanum tuberosum - enzymology</subject><subject>Storage and secretion, pigments, phytochrome</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LE0EQhgdR1uzq0ZtCE8RbYvd0T38IHpaYrEJA8OM81HSqkw6T7mz3TDD7h_ybjiYEPXmqot7nrXqhiuIFo1PGqHi73w-VT8WUCa4eFSNW8XJSVkI_LkaUDj3V2jwtrnPeUkoZZ-KquDKMlUqLUfFztoEEtsPkH6DzMZDoyKKFQwyxJV-PodtARgJhRZbY2wjDINojBL_ygXzw8cdxjeE3cocBMxmGtwkav4r77PM7suhTt8FE5ge_wmCRuJgGm3OYMHQeWvIF1317OT2eQ2qP4z8Hx0vocHxa_Kx44qDN-Pxcb4rvi_m32cfJ8vPdp9ntcrLlgnUTh7REik0joTGN084oZRoracOVdroCdNSySorKotKKgWw4VYK5yihBHfCb4v1p775vdriyQ8gEbb1PfgfpWEfw9b9K8Jt6HQ81q3Qp9eB_c_aneN9j7uqdzxbbFgLGPtdKG2kMr_4LMlmWmlVmAF_9negS5fzCQX991iFbaF2CYH2-YKU0XGo5YC9P2DZ3MV1kUSolpeK_APwMt8c</recordid><startdate>19970401</startdate><enddate>19970401</enddate><creator>Matthew K. Pelletier</creator><creator>Murrell, Julie R.</creator><creator>Shirley, Brenda W.</creator><general>American Society of Plant Physiologists</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970401</creationdate><title>Characterization of Flavonol Synthase and Leucoanthocyanidin Dioxygenase Genes in Arabidopsis: Further Evidence for Differential Regulation of "Early" and "Late" Genes</title><author>Matthew K. Pelletier ; Murrell, Julie R. ; Shirley, Brenda W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j341t-fe02e0ebb6ab9bf8f9779bc60b378f85aef0c15645ce7871a6b30741f59740fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - genetics</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Darkness</topic><topic>DNA</topic><topic>Enzymes</topic><topic>Flavonoids</topic><topic>Flavonols</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Regulation and Molecular Genetics</topic><topic>Genes, Plant</topic><topic>Genes. Genome</topic><topic>Genomics</topic><topic>Information Systems</topic><topic>Light</topic><topic>Messenger RNA</topic><topic>Metabolism</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Oxidoreductases - biosynthesis</topic><topic>Oxidoreductases - chemistry</topic><topic>Oxidoreductases - genetics</topic><topic>Oxygenases - biosynthesis</topic><topic>Oxygenases - chemistry</topic><topic>Oxygenases - genetics</topic><topic>Plant physiology and development</topic><topic>Plant Proteins</topic><topic>Plants</topic><topic>Plants - enzymology</topic><topic>RNA</topic><topic>Seedlings</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Tagged Sites</topic><topic>Solanum tuberosum - enzymology</topic><topic>Storage and secretion, pigments, phytochrome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matthew K. Pelletier</creatorcontrib><creatorcontrib>Murrell, Julie R.</creatorcontrib><creatorcontrib>Shirley, Brenda W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matthew K. Pelletier</au><au>Murrell, Julie R.</au><au>Shirley, Brenda W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Flavonol Synthase and Leucoanthocyanidin Dioxygenase Genes in Arabidopsis: Further Evidence for Differential Regulation of "Early" and "Late" Genes</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1997-04-01</date><risdate>1997</risdate><volume>113</volume><issue>4</issue><spage>1437</spage><epage>1445</epage><pages>1437-1445</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>As part of an ongoing investigation into the organization and regulation of the flavonoid biosynthetic pathway, two Arabidopsis thaliana expressed sequence tag (EST) clones (153O10T7 and YAY780) with high homology to leucoanthocyanidin dioxygenase (LDOX) or flavonol synthase (FLS) were identified. EST YAY780 was sequenced and found to encode a protein 49 to 78% identical to all LDOX sequences in the database. EST 153O10T7 was used to isolate a genomic clone encoding a protein with 59 to 61% sequence identity to petunia (Petunia hybrida) and potato (Solanum tuberosum) FLS. DNA blot analysis was used to screen the Arabidopsis genome for sequences related to FLS and LDOX and to determine the positions of the two clones on the RI map. The expression patterns of FLS and LDOX in etiolated seedlings moved to white light and in two putative regulatory mutants (ttg and tt8) were determined by RNA blot analysis. These studies indicate that FLS is an "early" flavonoid gene in Arabidopsis seedlings, whereas LDOX is a "late" gene. Furthermore, FLS is the first flavonoid enzyme identified in Arabidopsis that may be encoded by a gene family.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>9112784</pmid><doi>10.1104/pp.113.4.1437</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1997-04, Vol.113 (4), p.1437-1445 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_158268 |
| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Amino acids Arabidopsis - enzymology Arabidopsis - genetics Biological and medical sciences Cloning, Molecular Darkness DNA Enzymes Flavonoids Flavonols Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Gene Regulation and Molecular Genetics Genes, Plant Genes. Genome Genomics Information Systems Light Messenger RNA Metabolism Molecular and cellular biology Molecular genetics Molecular Sequence Data Multigene Family Oxidoreductases - biosynthesis Oxidoreductases - chemistry Oxidoreductases - genetics Oxygenases - biosynthesis Oxygenases - chemistry Oxygenases - genetics Plant physiology and development Plant Proteins Plants Plants - enzymology RNA Seedlings Sequence Homology, Amino Acid Sequence Tagged Sites Solanum tuberosum - enzymology Storage and secretion, pigments, phytochrome |
| title | Characterization of Flavonol Synthase and Leucoanthocyanidin Dioxygenase Genes in Arabidopsis: Further Evidence for Differential Regulation of "Early" and "Late" Genes |
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