Identification and characterization of linoleic acid as an endogenous modulator of in vitro N-1-naphthylphthalamic acid binding
An endogenous inhibitor of the in vitro binding of the phytotropin N-1-naphthylphthalamic acid to microsomal membranes was detected in extracts prepared from etiolated pea (Pisum sativum L.) epicotyls. Following extensive purification, the inhibitor was identified as linoleic acid. Authentic linolei...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1997-02, Vol.113 (2), p.519-525 |
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| creator | Suttle, J.C. (USDA, Northern Crop Science Laboratory, Fargo, ND.) |
| description | An endogenous inhibitor of the in vitro binding of the phytotropin N-1-naphthylphthalamic acid to microsomal membranes was detected in extracts prepared from etiolated pea (Pisum sativum L.) epicotyls. Following extensive purification, the inhibitor was identified as linoleic acid. Authentic linoleic acid inhibited N-1-naphthylphthalamic acid binding noncompetitively in a dose-dependent manner, exhibiting a 50% inhibitory concentration of approximately 24 micromolar. Using a variety of fatty acids and their derivatives, this inhibition was found to exhibit strict structural requirements, with both linoleic and linolenic acids being the most inhibitory. A variety of membrane-solubilizing detergents elicited no such inhibitory activity when tested at equivalent concentrations. The possible physiological significance of this interaction is discussed and it is proposed that linoleic acid serves as an intracellular modulator of phytotropin binding and therefore polar auxin transport |
| doi_str_mv | 10.1104/pp.113.2.519 |
| format | Article |
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The possible physiological significance of this interaction is discussed and it is proposed that linoleic acid serves as an intracellular modulator of phytotropin binding and therefore polar auxin transport</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.113.2.519</identifier><identifier>PMID: 12223622</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ACIDE GRAS ; ACIDE LINOLEIQUE ; ACIDO LINOLEICO ; ACIDOS GRASOS ; AUXINAS ; AUXINE ; Auxins ; Biological and medical sciences ; Cell biochemistry ; Cell Biology and Signal Transduction ; Cell physiology ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; Conjugated linoleic acids ; CULTIVO IN VITRO ; CULTURE IN VITRO ; DESINFECTANT ; DESINFECTANTES ; EPICOTILOS ; EPICOTYLE ; Epicotyls ; ESTER ; ESTERES ; Fatty acids ; Fundamental and applied biological sciences. Psychology ; Ligands ; Microsomes ; Nonesterified fatty acids ; ORGANITE CELLULAIRE ; ORGANULOS CITOPLASMICOS ; Peas ; PISUM SATIVUM ; Plant physiology and development ; PURIFICACION ; PURIFICATION ; Receptors ; TRANSPORT DES SUBSTANCES NUTRITIVES ; TRANSPORTE DE NUTRIENTES ; Unsaturated fatty acids</subject><ispartof>Plant physiology (Bethesda), 1997-02, Vol.113 (2), p.519-525</ispartof><rights>Copyright 1997 American Society of Plant Physiologists</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4277561$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4277561$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2587610$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12223622$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suttle, J.C. (USDA, Northern Crop Science Laboratory, Fargo, ND.)</creatorcontrib><title>Identification and characterization of linoleic acid as an endogenous modulator of in vitro N-1-naphthylphthalamic acid binding</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>An endogenous inhibitor of the in vitro binding of the phytotropin N-1-naphthylphthalamic acid to microsomal membranes was detected in extracts prepared from etiolated pea (Pisum sativum L.) epicotyls. Following extensive purification, the inhibitor was identified as linoleic acid. Authentic linoleic acid inhibited N-1-naphthylphthalamic acid binding noncompetitively in a dose-dependent manner, exhibiting a 50% inhibitory concentration of approximately 24 micromolar. Using a variety of fatty acids and their derivatives, this inhibition was found to exhibit strict structural requirements, with both linoleic and linolenic acids being the most inhibitory. A variety of membrane-solubilizing detergents elicited no such inhibitory activity when tested at equivalent concentrations. The possible physiological significance of this interaction is discussed and it is proposed that linoleic acid serves as an intracellular modulator of phytotropin binding and therefore polar auxin transport</description><subject>ACIDE GRAS</subject><subject>ACIDE LINOLEIQUE</subject><subject>ACIDO LINOLEICO</subject><subject>ACIDOS GRASOS</subject><subject>AUXINAS</subject><subject>AUXINE</subject><subject>Auxins</subject><subject>Biological and medical sciences</subject><subject>Cell biochemistry</subject><subject>Cell Biology and Signal Transduction</subject><subject>Cell physiology</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>Conjugated linoleic acids</subject><subject>CULTIVO IN VITRO</subject><subject>CULTURE IN VITRO</subject><subject>DESINFECTANT</subject><subject>DESINFECTANTES</subject><subject>EPICOTILOS</subject><subject>EPICOTYLE</subject><subject>Epicotyls</subject><subject>ESTER</subject><subject>ESTERES</subject><subject>Fatty acids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Ligands</subject><subject>Microsomes</subject><subject>Nonesterified fatty acids</subject><subject>ORGANITE CELLULAIRE</subject><subject>ORGANULOS CITOPLASMICOS</subject><subject>Peas</subject><subject>PISUM SATIVUM</subject><subject>Plant physiology and development</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>Receptors</subject><subject>TRANSPORT DES SUBSTANCES NUTRITIVES</subject><subject>TRANSPORTE DE NUTRIENTES</subject><subject>Unsaturated fatty acids</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNpVkT2P1DAQhiME4paDjgohlIKCgiweO_5IcQU68XHSCQq42prYzq5PWTvY2ZOOhr-OV1kWaDyW32feGc9U1XMgawDSvpumEtmarjl0D6oVcEYbylv1sFoRUu5Eqe6sepLzLSEEGLSPqzOglDJB6ar6dWVdmP3gDc4-hhqDrc0WE5rZJf9zeYxDPfoQR-dNjcbbGnMBaxds3LgQ97neRbsfcY7pwPpQ3_k5xfpLA03AaTtv78fDiSPu_lj0PlgfNk-rRwOO2T07xvPq5uOH75efm-uvn64u3183pm3Z3BhKECkMnWNKgbUSeg6iExL7Xgnec0OlpS3wziAB2fcESZlHP0hlZc8ZO68uFt9p3--cNeXTCUc9Jb_DdK8jev2_EvxWb-KdBq5AqJL_5pif4o-9y7Pe-WzcOGJwZQAaFO-YFFR0BX27oCbFnJMbTlWA6MPK9DSVyDTVpcWCv_q3s7_wcUcFeH0EMBsch4TB-HziKFdSACnYywW7zWUPJ7mlUnIBRX6xyANGjZtUHG6-dZIJJgj7DZe5suw</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Suttle, J.C. (USDA, Northern Crop Science Laboratory, Fargo, ND.)</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970201</creationdate><title>Identification and characterization of linoleic acid as an endogenous modulator of in vitro N-1-naphthylphthalamic acid binding</title><author>Suttle, J.C. (USDA, Northern Crop Science Laboratory, Fargo, ND.)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-c20aa21f9e3881dd71b516967abb865b5c27d24159ca017bb0a0519bf78d7b533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>ACIDE GRAS</topic><topic>ACIDE LINOLEIQUE</topic><topic>ACIDO LINOLEICO</topic><topic>ACIDOS GRASOS</topic><topic>AUXINAS</topic><topic>AUXINE</topic><topic>Auxins</topic><topic>Biological and medical sciences</topic><topic>Cell biochemistry</topic><topic>Cell Biology and Signal Transduction</topic><topic>Cell physiology</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>Conjugated linoleic acids</topic><topic>CULTIVO IN VITRO</topic><topic>CULTURE IN VITRO</topic><topic>DESINFECTANT</topic><topic>DESINFECTANTES</topic><topic>EPICOTILOS</topic><topic>EPICOTYLE</topic><topic>Epicotyls</topic><topic>ESTER</topic><topic>ESTERES</topic><topic>Fatty acids</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Ligands</topic><topic>Microsomes</topic><topic>Nonesterified fatty acids</topic><topic>ORGANITE CELLULAIRE</topic><topic>ORGANULOS CITOPLASMICOS</topic><topic>Peas</topic><topic>PISUM SATIVUM</topic><topic>Plant physiology and development</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>Receptors</topic><topic>TRANSPORT DES SUBSTANCES NUTRITIVES</topic><topic>TRANSPORTE DE NUTRIENTES</topic><topic>Unsaturated fatty acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suttle, J.C. (USDA, Northern Crop Science Laboratory, Fargo, ND.)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suttle, J.C. (USDA, Northern Crop Science Laboratory, Fargo, ND.)</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of linoleic acid as an endogenous modulator of in vitro N-1-naphthylphthalamic acid binding</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>113</volume><issue>2</issue><spage>519</spage><epage>525</epage><pages>519-525</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>An endogenous inhibitor of the in vitro binding of the phytotropin N-1-naphthylphthalamic acid to microsomal membranes was detected in extracts prepared from etiolated pea (Pisum sativum L.) epicotyls. Following extensive purification, the inhibitor was identified as linoleic acid. Authentic linoleic acid inhibited N-1-naphthylphthalamic acid binding noncompetitively in a dose-dependent manner, exhibiting a 50% inhibitory concentration of approximately 24 micromolar. Using a variety of fatty acids and their derivatives, this inhibition was found to exhibit strict structural requirements, with both linoleic and linolenic acids being the most inhibitory. A variety of membrane-solubilizing detergents elicited no such inhibitory activity when tested at equivalent concentrations. The possible physiological significance of this interaction is discussed and it is proposed that linoleic acid serves as an intracellular modulator of phytotropin binding and therefore polar auxin transport</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>12223622</pmid><doi>10.1104/pp.113.2.519</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | ACIDE GRAS ACIDE LINOLEIQUE ACIDO LINOLEICO ACIDOS GRASOS AUXINAS AUXINE Auxins Biological and medical sciences Cell biochemistry Cell Biology and Signal Transduction Cell physiology COMPOSICION QUIMICA COMPOSITION CHIMIQUE Conjugated linoleic acids CULTIVO IN VITRO CULTURE IN VITRO DESINFECTANT DESINFECTANTES EPICOTILOS EPICOTYLE Epicotyls ESTER ESTERES Fatty acids Fundamental and applied biological sciences. Psychology Ligands Microsomes Nonesterified fatty acids ORGANITE CELLULAIRE ORGANULOS CITOPLASMICOS Peas PISUM SATIVUM Plant physiology and development PURIFICACION PURIFICATION Receptors TRANSPORT DES SUBSTANCES NUTRITIVES TRANSPORTE DE NUTRIENTES Unsaturated fatty acids |
| title | Identification and characterization of linoleic acid as an endogenous modulator of in vitro N-1-naphthylphthalamic acid binding |
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