Cinnamyl Alcohol Dehydrogenase: Identification of New Sites of Promoter Activity in Transgenic Poplar
Stem sections from poplar that were stably transformed with a eucalypt cinnamyl alcohol dehydrogenase promoter-β-glucuronidase construct were prepared by using either a technique routinely used in herbaceous species or a technique designed to take into account the particular anatomy of woody plants....
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Veröffentlicht in: | Plant physiology (Bethesda) 1997-02, Vol.113 (2), p.321-325 |
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creator | Simon Hawkins Jozef Samaj Lauvergeat, Virginie Boudet, Alain Grima-Pettenati, Jacqueline |
description | Stem sections from poplar that were stably transformed with a eucalypt cinnamyl alcohol dehydrogenase promoter-β-glucuronidase construct were prepared by using either a technique routinely used in herbaceous species or a technique designed to take into account the particular anatomy of woody plants. Although both preparation techniques confirmed the pattern of expression previously observed (C. Feuillet, V. Lauvergeat, C. Deswarte, G. Pilate, A. Boudet and J. Grima-Pettenati [1995] Plant Mol Biol 27: 651-657), the latter technique also allowed the detection of other sites of promoter activity not revealed by the first technique. In situ hybridization confirmed the expression pattern obtained with the second sample preparation technique. |
doi_str_mv | 10.1104/pp.113.2.321 |
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Psychology</subject><subject>In situ hybridization</subject><subject>Lignification</subject><subject>Lignin</subject><subject>Metabolism</subject><subject>Parenchyma</subject><subject>Periderm</subject><subject>Phloem</subject><subject>Plant physiology and development</subject><subject>Rapid Communication</subject><subject>Xylem</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNp9kcFrFDEUxoModrt68yiSg0gP7pqXZGYygodlrbZQtGA9h0z2TTdlZjJNsi3735t2l9VehMCX8H7v48t7hLwBNgdg8tM4ZhVzPhccnpEJFILPeCHVczJhLN-ZUvUROY7xhjEGAuRLcgScc1ECmxBcumEw_baji876te_oV1xvV8Ff42AifqbnKxySa501yfmB-pb-wHv6yyWMD4_L4HufMNCFTe7OpS11A70KZojZwFl66cfOhFfkRWu6iK_3OiW_v51eLc9mFz-_ny8XFzMra5VmpTIVV7VRlZWiwbquGlQgUJbGYtXaxhYtWuCrRoFRTSllIWw-LQhRKmHFlHzZ-Y6bpseVzdGD6fQYXG_CVnvj9NPK4Nb62t9pKBRksyk52fcHf7vBmHTvosWuMwP6TdSgilpUZVmojH74P1oKVgGHDH7cgTb4GAO2hzjA9MMG9ThmFZpr8Yi_-_cLf-H9yjLwfg-YaE3X5llbFw8cL3LGR5-3O-wmJh8OZcmrqhBK_AHCm66t</recordid><startdate>19970201</startdate><enddate>19970201</enddate><creator>Simon Hawkins</creator><creator>Jozef Samaj</creator><creator>Lauvergeat, Virginie</creator><creator>Boudet, Alain</creator><creator>Grima-Pettenati, Jacqueline</creator><general>American Society of Plant Physiologists</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19970201</creationdate><title>Cinnamyl Alcohol Dehydrogenase: Identification of New Sites of Promoter Activity in Transgenic Poplar</title><author>Simon Hawkins ; Jozef Samaj ; Lauvergeat, Virginie ; Boudet, Alain ; Grima-Pettenati, Jacqueline</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-68a7289a87c43be997be813e46ace7fcbc5fec12db81a8b64453c53cf133683c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Alcohols</topic><topic>Biological and medical sciences</topic><topic>Dehydrogenases</topic><topic>Enzymes</topic><topic>Fiber cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In situ hybridization</topic><topic>Lignification</topic><topic>Lignin</topic><topic>Metabolism</topic><topic>Parenchyma</topic><topic>Periderm</topic><topic>Phloem</topic><topic>Plant physiology and development</topic><topic>Rapid Communication</topic><topic>Xylem</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simon Hawkins</creatorcontrib><creatorcontrib>Jozef Samaj</creatorcontrib><creatorcontrib>Lauvergeat, Virginie</creatorcontrib><creatorcontrib>Boudet, Alain</creatorcontrib><creatorcontrib>Grima-Pettenati, Jacqueline</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simon Hawkins</au><au>Jozef Samaj</au><au>Lauvergeat, Virginie</au><au>Boudet, Alain</au><au>Grima-Pettenati, Jacqueline</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cinnamyl Alcohol Dehydrogenase: Identification of New Sites of Promoter Activity in Transgenic Poplar</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1997-02-01</date><risdate>1997</risdate><volume>113</volume><issue>2</issue><spage>321</spage><epage>325</epage><pages>321-325</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Stem sections from poplar that were stably transformed with a eucalypt cinnamyl alcohol dehydrogenase promoter-β-glucuronidase construct were prepared by using either a technique routinely used in herbaceous species or a technique designed to take into account the particular anatomy of woody plants. 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source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current) |
subjects | Alcohols Biological and medical sciences Dehydrogenases Enzymes Fiber cells Fundamental and applied biological sciences. Psychology In situ hybridization Lignification Lignin Metabolism Parenchyma Periderm Phloem Plant physiology and development Rapid Communication Xylem |
title | Cinnamyl Alcohol Dehydrogenase: Identification of New Sites of Promoter Activity in Transgenic Poplar |
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