Influence of gene dosage on carbohydrate synthesis and enzymatic activities in endosperm of starch-deficient mutants of maize
In cereals, starch is synthesized in endosperm cells, which have a ploidy level of three. By studying the allelic dosage of mutants affecting starch formation in maize (Zea mays L.) kernels, we determined the effect of down-regulated enzyme activity on starch accumulation and the activity of associa...
Gespeichert in:
| Veröffentlicht in: | Plant physiology (Bethesda) 1997-01, Vol.113 (1), p.293-304 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 304 |
|---|---|
| container_issue | 1 |
| container_start_page | 293 |
| container_title | Plant physiology (Bethesda) |
| container_volume | 113 |
| creator | Singletary, G.W. (Pioneer Hi-Bred International, Johnston, IA.) Banisadr, R Keeling, P.L |
| description | In cereals, starch is synthesized in endosperm cells, which have a ploidy level of three. By studying the allelic dosage of mutants affecting starch formation in maize (Zea mays L.) kernels, we determined the effect of down-regulated enzyme activity on starch accumulation and the activity of associated enzymes of carbohydrate metabolism. We found a direct relationship between the amount of starch produced in the endosperm and the gene dosage of amylose extender-1, brittle-2, shrunken1, and sugary-1 mutant alleles. Changes in starch content were found to be caused by changes in the duration as well as the rate of starch synthesis, depending on the mutant. Branching enzyme, ADP-glucose pyrophosphorylase, and sucrose synthase activities were linearly reduced in endosperm containing increasing dosages of amylose extender-1, brittle-2, and shrunken-1 alleles, respectively. Debranching enzyme activity declined only in the presence of two or three copies of sugary-1. No enzyme-dosage relationship occurred with the dull1 mutant allele. All mutants except sugary-1 displayed large increases (approximately 2- to 5-fold) in activity among various enzymes unrelated to the structural gene. This occurred in homozygous recessive genotypes, as did elevated concentrations of soluble sugars, and differed in magnitude and distribution among enzymes according to the particular mutation |
| doi_str_mv | 10.1104/pp.113.1.293 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_158141</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>4277532</jstor_id><sourcerecordid>4277532</sourcerecordid><originalsourceid>FETCH-LOGICAL-c583t-d5aedb9146cded4fc4c7ac82fdd186cd7b9d7268217f15a63a869066cb5a83cc3</originalsourceid><addsrcrecordid>eNp9ks2LFDEQxYMo7rh68yQiOYh4sMd8dCfpgwdZVl1Y8KB7DtVJeiZLd7pN0guz4P--GWYY9eLpJfV-9ahQQeglJWtKSf1xnovyNV2zlj9CK9pwVrGmVo_RipByJkq1Z-hZSreEEMpp_RSdUcYYF0Su0O-r0A-LC8bhqccbFxy2U4JNuQZsIHbTdmcjZIfTLuStSz5hCBa7cL8bIXuDwWR_57N3CftQ6qV9dnHcx6UM0Wwr63pvvAsZj0uGkNPeG8Hfu-foSQ9Dci-Oeo5uvlz-vPhWXX__enXx-boyjeK5sg0427W0FsY6W_emNhKMYr21VJWa7FormVCMyp42IDgo0RIhTNeA4sbwc_TpkDsv3eisKbNEGPQc_Qhxpyfw-l8n-K3eTHeaNorWtPS_P_bH6dfiUtajT8YNAwQ3LUlT1bRcCib36Lv_o4KpMlpTwA8H0MQppej60ziU6P1m9TwX5ZrqstmCv_n7CX_g4yoL8PYIQDIw9BGC8enEsUZQVZOCvT5gtylP8WTXTMryc4r96mD3MGnYxJJw86OVVHDJ-AMD88E-</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16280665</pqid></control><display><type>article</type><title>Influence of gene dosage on carbohydrate synthesis and enzymatic activities in endosperm of starch-deficient mutants of maize</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>JSTOR Archive Collection A-Z Listing</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Singletary, G.W. (Pioneer Hi-Bred International, Johnston, IA.) ; Banisadr, R ; Keeling, P.L</creator><creatorcontrib>Singletary, G.W. (Pioneer Hi-Bred International, Johnston, IA.) ; Banisadr, R ; Keeling, P.L</creatorcontrib><description>In cereals, starch is synthesized in endosperm cells, which have a ploidy level of three. By studying the allelic dosage of mutants affecting starch formation in maize (Zea mays L.) kernels, we determined the effect of down-regulated enzyme activity on starch accumulation and the activity of associated enzymes of carbohydrate metabolism. We found a direct relationship between the amount of starch produced in the endosperm and the gene dosage of amylose extender-1, brittle-2, shrunken1, and sugary-1 mutant alleles. Changes in starch content were found to be caused by changes in the duration as well as the rate of starch synthesis, depending on the mutant. Branching enzyme, ADP-glucose pyrophosphorylase, and sucrose synthase activities were linearly reduced in endosperm containing increasing dosages of amylose extender-1, brittle-2, and shrunken-1 alleles, respectively. Debranching enzyme activity declined only in the presence of two or three copies of sugary-1. No enzyme-dosage relationship occurred with the dull1 mutant allele. All mutants except sugary-1 displayed large increases (approximately 2- to 5-fold) in activity among various enzymes unrelated to the structural gene. This occurred in homozygous recessive genotypes, as did elevated concentrations of soluble sugars, and differed in magnitude and distribution among enzymes according to the particular mutation</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.113.1.293</identifier><identifier>PMID: 12223607</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; ADENOSINDIFOSFATO ; ADENOSINE DIPHOSPHATE ; Alleles ; ALMIDON ; AMIDON ; ANALISIS CUANTITATIVO ; ANALYSE QUANTITATIVE ; Biochemistry and Enzymology ; Biological and medical sciences ; BIOSINTESIS ; BIOSYNTHESE ; CARBOHIDRATOS ; CARENCE EN SUBSTANCE NUTRITIVE ; Corn ; DEFICIENCIAS NUTRITIVAS ; Endosperm ; ENDOSPERMA ; ENDOSPERME ; Enzyme activity ; Enzymes ; FOSFORILACION ; Fundamental and applied biological sciences. Psychology ; GENE ; Gene dosage ; GENES ; GENETICA ; GENETIQUE ; GENOTIPOS ; GENOTYPE ; Genotypes ; GLICOSILTRANSFERASAS ; GLUCIDE ; GLUCOSA ; GLUCOSE ; GLYCOSYLTRANSFERASE ; Metabolism ; METABOLISME DES GLUCIDES ; METABOLISMO DE CARBOHIDRATOS ; MUTANT ; MUTANTES ; PHOSPHORYLATION ; Photosynthesis, respiration. Anabolism, catabolism ; Plant physiology and development ; Plants ; Starches ; Sugars ; VARIACION GENETICA ; VARIATION GENETIQUE ; ZEA MAYS</subject><ispartof>Plant physiology (Bethesda), 1997-01, Vol.113 (1), p.293-304</ispartof><rights>Copyright 1997 American Society of Plant Physiologists</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c583t-d5aedb9146cded4fc4c7ac82fdd186cd7b9d7268217f15a63a869066cb5a83cc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4277532$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4277532$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,781,785,804,886,4025,27928,27929,27930,58022,58255</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2561840$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12223607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Singletary, G.W. (Pioneer Hi-Bred International, Johnston, IA.)</creatorcontrib><creatorcontrib>Banisadr, R</creatorcontrib><creatorcontrib>Keeling, P.L</creatorcontrib><title>Influence of gene dosage on carbohydrate synthesis and enzymatic activities in endosperm of starch-deficient mutants of maize</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>In cereals, starch is synthesized in endosperm cells, which have a ploidy level of three. By studying the allelic dosage of mutants affecting starch formation in maize (Zea mays L.) kernels, we determined the effect of down-regulated enzyme activity on starch accumulation and the activity of associated enzymes of carbohydrate metabolism. We found a direct relationship between the amount of starch produced in the endosperm and the gene dosage of amylose extender-1, brittle-2, shrunken1, and sugary-1 mutant alleles. Changes in starch content were found to be caused by changes in the duration as well as the rate of starch synthesis, depending on the mutant. Branching enzyme, ADP-glucose pyrophosphorylase, and sucrose synthase activities were linearly reduced in endosperm containing increasing dosages of amylose extender-1, brittle-2, and shrunken-1 alleles, respectively. Debranching enzyme activity declined only in the presence of two or three copies of sugary-1. No enzyme-dosage relationship occurred with the dull1 mutant allele. All mutants except sugary-1 displayed large increases (approximately 2- to 5-fold) in activity among various enzymes unrelated to the structural gene. This occurred in homozygous recessive genotypes, as did elevated concentrations of soluble sugars, and differed in magnitude and distribution among enzymes according to the particular mutation</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>ADENOSINDIFOSFATO</subject><subject>ADENOSINE DIPHOSPHATE</subject><subject>Alleles</subject><subject>ALMIDON</subject><subject>AMIDON</subject><subject>ANALISIS CUANTITATIVO</subject><subject>ANALYSE QUANTITATIVE</subject><subject>Biochemistry and Enzymology</subject><subject>Biological and medical sciences</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>CARBOHIDRATOS</subject><subject>CARENCE EN SUBSTANCE NUTRITIVE</subject><subject>Corn</subject><subject>DEFICIENCIAS NUTRITIVAS</subject><subject>Endosperm</subject><subject>ENDOSPERMA</subject><subject>ENDOSPERME</subject><subject>Enzyme activity</subject><subject>Enzymes</subject><subject>FOSFORILACION</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>Gene dosage</subject><subject>GENES</subject><subject>GENETICA</subject><subject>GENETIQUE</subject><subject>GENOTIPOS</subject><subject>GENOTYPE</subject><subject>Genotypes</subject><subject>GLICOSILTRANSFERASAS</subject><subject>GLUCIDE</subject><subject>GLUCOSA</subject><subject>GLUCOSE</subject><subject>GLYCOSYLTRANSFERASE</subject><subject>Metabolism</subject><subject>METABOLISME DES GLUCIDES</subject><subject>METABOLISMO DE CARBOHIDRATOS</subject><subject>MUTANT</subject><subject>MUTANTES</subject><subject>PHOSPHORYLATION</subject><subject>Photosynthesis, respiration. Anabolism, catabolism</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>Starches</subject><subject>Sugars</subject><subject>VARIACION GENETICA</subject><subject>VARIATION GENETIQUE</subject><subject>ZEA MAYS</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><recordid>eNp9ks2LFDEQxYMo7rh68yQiOYh4sMd8dCfpgwdZVl1Y8KB7DtVJeiZLd7pN0guz4P--GWYY9eLpJfV-9ahQQeglJWtKSf1xnovyNV2zlj9CK9pwVrGmVo_RipByJkq1Z-hZSreEEMpp_RSdUcYYF0Su0O-r0A-LC8bhqccbFxy2U4JNuQZsIHbTdmcjZIfTLuStSz5hCBa7cL8bIXuDwWR_57N3CftQ6qV9dnHcx6UM0Wwr63pvvAsZj0uGkNPeG8Hfu-foSQ9Dci-Oeo5uvlz-vPhWXX__enXx-boyjeK5sg0427W0FsY6W_emNhKMYr21VJWa7FormVCMyp42IDgo0RIhTNeA4sbwc_TpkDsv3eisKbNEGPQc_Qhxpyfw-l8n-K3eTHeaNorWtPS_P_bH6dfiUtajT8YNAwQ3LUlT1bRcCib36Lv_o4KpMlpTwA8H0MQppej60ziU6P1m9TwX5ZrqstmCv_n7CX_g4yoL8PYIQDIw9BGC8enEsUZQVZOCvT5gtylP8WTXTMryc4r96mD3MGnYxJJw86OVVHDJ-AMD88E-</recordid><startdate>199701</startdate><enddate>199701</enddate><creator>Singletary, G.W. (Pioneer Hi-Bred International, Johnston, IA.)</creator><creator>Banisadr, R</creator><creator>Keeling, P.L</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>199701</creationdate><title>Influence of gene dosage on carbohydrate synthesis and enzymatic activities in endosperm of starch-deficient mutants of maize</title><author>Singletary, G.W. (Pioneer Hi-Bred International, Johnston, IA.) ; Banisadr, R ; Keeling, P.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c583t-d5aedb9146cded4fc4c7ac82fdd186cd7b9d7268217f15a63a869066cb5a83cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>ADENOSINDIFOSFATO</topic><topic>ADENOSINE DIPHOSPHATE</topic><topic>Alleles</topic><topic>ALMIDON</topic><topic>AMIDON</topic><topic>ANALISIS CUANTITATIVO</topic><topic>ANALYSE QUANTITATIVE</topic><topic>Biochemistry and Enzymology</topic><topic>Biological and medical sciences</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>CARBOHIDRATOS</topic><topic>CARENCE EN SUBSTANCE NUTRITIVE</topic><topic>Corn</topic><topic>DEFICIENCIAS NUTRITIVAS</topic><topic>Endosperm</topic><topic>ENDOSPERMA</topic><topic>ENDOSPERME</topic><topic>Enzyme activity</topic><topic>Enzymes</topic><topic>FOSFORILACION</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>Gene dosage</topic><topic>GENES</topic><topic>GENETICA</topic><topic>GENETIQUE</topic><topic>GENOTIPOS</topic><topic>GENOTYPE</topic><topic>Genotypes</topic><topic>GLICOSILTRANSFERASAS</topic><topic>GLUCIDE</topic><topic>GLUCOSA</topic><topic>GLUCOSE</topic><topic>GLYCOSYLTRANSFERASE</topic><topic>Metabolism</topic><topic>METABOLISME DES GLUCIDES</topic><topic>METABOLISMO DE CARBOHIDRATOS</topic><topic>MUTANT</topic><topic>MUTANTES</topic><topic>PHOSPHORYLATION</topic><topic>Photosynthesis, respiration. Anabolism, catabolism</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>Starches</topic><topic>Sugars</topic><topic>VARIACION GENETICA</topic><topic>VARIATION GENETIQUE</topic><topic>ZEA MAYS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Singletary, G.W. (Pioneer Hi-Bred International, Johnston, IA.)</creatorcontrib><creatorcontrib>Banisadr, R</creatorcontrib><creatorcontrib>Keeling, P.L</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Singletary, G.W. (Pioneer Hi-Bred International, Johnston, IA.)</au><au>Banisadr, R</au><au>Keeling, P.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of gene dosage on carbohydrate synthesis and enzymatic activities in endosperm of starch-deficient mutants of maize</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1997-01</date><risdate>1997</risdate><volume>113</volume><issue>1</issue><spage>293</spage><epage>304</epage><pages>293-304</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>In cereals, starch is synthesized in endosperm cells, which have a ploidy level of three. By studying the allelic dosage of mutants affecting starch formation in maize (Zea mays L.) kernels, we determined the effect of down-regulated enzyme activity on starch accumulation and the activity of associated enzymes of carbohydrate metabolism. We found a direct relationship between the amount of starch produced in the endosperm and the gene dosage of amylose extender-1, brittle-2, shrunken1, and sugary-1 mutant alleles. Changes in starch content were found to be caused by changes in the duration as well as the rate of starch synthesis, depending on the mutant. Branching enzyme, ADP-glucose pyrophosphorylase, and sucrose synthase activities were linearly reduced in endosperm containing increasing dosages of amylose extender-1, brittle-2, and shrunken-1 alleles, respectively. Debranching enzyme activity declined only in the presence of two or three copies of sugary-1. No enzyme-dosage relationship occurred with the dull1 mutant allele. All mutants except sugary-1 displayed large increases (approximately 2- to 5-fold) in activity among various enzymes unrelated to the structural gene. This occurred in homozygous recessive genotypes, as did elevated concentrations of soluble sugars, and differed in magnitude and distribution among enzymes according to the particular mutation</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>12223607</pmid><doi>10.1104/pp.113.1.293</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0889 |
| ispartof | Plant physiology (Bethesda), 1997-01, Vol.113 (1), p.293-304 |
| issn | 0032-0889 1532-2548 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_158141 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current) |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE ADENOSINDIFOSFATO ADENOSINE DIPHOSPHATE Alleles ALMIDON AMIDON ANALISIS CUANTITATIVO ANALYSE QUANTITATIVE Biochemistry and Enzymology Biological and medical sciences BIOSINTESIS BIOSYNTHESE CARBOHIDRATOS CARENCE EN SUBSTANCE NUTRITIVE Corn DEFICIENCIAS NUTRITIVAS Endosperm ENDOSPERMA ENDOSPERME Enzyme activity Enzymes FOSFORILACION Fundamental and applied biological sciences. Psychology GENE Gene dosage GENES GENETICA GENETIQUE GENOTIPOS GENOTYPE Genotypes GLICOSILTRANSFERASAS GLUCIDE GLUCOSA GLUCOSE GLYCOSYLTRANSFERASE Metabolism METABOLISME DES GLUCIDES METABOLISMO DE CARBOHIDRATOS MUTANT MUTANTES PHOSPHORYLATION Photosynthesis, respiration. Anabolism, catabolism Plant physiology and development Plants Starches Sugars VARIACION GENETICA VARIATION GENETIQUE ZEA MAYS |
| title | Influence of gene dosage on carbohydrate synthesis and enzymatic activities in endosperm of starch-deficient mutants of maize |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-15T20%3A00%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Influence%20of%20gene%20dosage%20on%20carbohydrate%20synthesis%20and%20enzymatic%20activities%20in%20endosperm%20of%20starch-deficient%20mutants%20of%20maize&rft.jtitle=Plant%20physiology%20(Bethesda)&rft.au=Singletary,%20G.W.%20(Pioneer%20Hi-Bred%20International,%20Johnston,%20IA.)&rft.date=1997-01&rft.volume=113&rft.issue=1&rft.spage=293&rft.epage=304&rft.pages=293-304&rft.issn=0032-0889&rft.eissn=1532-2548&rft.coden=PPHYA5&rft_id=info:doi/10.1104/pp.113.1.293&rft_dat=%3Cjstor_pubme%3E4277532%3C/jstor_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16280665&rft_id=info:pmid/12223607&rft_jstor_id=4277532&rfr_iscdi=true |