Intracellular localization of phospholipase D in leaves and seedling tissues of castor bean
The intracellular distribution of phospholipase D (PLD; EC 31.4.4) in castor bean (Ricinus communis L.) tissues was investigated by subcellular fractionation and by immuno-electron microscopy. Centrifugal fractionation revealed that most PLD in young leaves was soluble, whereas in mature leaves a ma...
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| Veröffentlicht in: | Plant physiology (Bethesda) 1996-05, Vol.111 (1), p.101-107 |
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| creator | Xu, L. (Kansas State University, Manhattan, KS.) Paulsen, A.Q Ryu, S.B Wang, X |
| description | The intracellular distribution of phospholipase D (PLD; EC 31.4.4) in castor bean (Ricinus communis L.) tissues was investigated by subcellular fractionation and by immuno-electron microscopy. Centrifugal fractionation revealed that most PLD in young leaves was soluble, whereas in mature leaves a majority of PLD was associated with microsomal membranes. Further separation of microsomal membranes by a two-phase partitioning system indicated that PLD was associated with both plasma and intracellular membranes. Sucrose gradient separation of intracellular membranes showed PLD present in the endoplasmic reticulum, a submicrosomal band, and in soluble fractions but not in mitochondria and glyoxysomes of postgermination endosperm. Immunocytochemical studies found high gold labeling in vacuoles in young leaves, suggesting that the high level of soluble PLD in young leaves is due to release of PLD from vacuoles during tissue disruption. In addition to the labeling in vacuoles, gold particles were also found in the cytoplasmic matrices and plasma membrane in leaves and in 2-d postgermination seedlings. Collectively, these results show that PLD in castor bean leaf and seedling tissues is localized in the vacuole and is associated with the endoplasmic reticulum and plasma membrane and that the relative distribution between the soluble and membrane compartments changes during castor bean leaf development |
| doi_str_mv | 10.1104/pp.111.1.101 |
| format | Article |
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(Kansas State University, Manhattan, KS.) ; Paulsen, A.Q ; Ryu, S.B ; Wang, X</creator><creatorcontrib>Xu, L. (Kansas State University, Manhattan, KS.) ; Paulsen, A.Q ; Ryu, S.B ; Wang, X</creatorcontrib><description>The intracellular distribution of phospholipase D (PLD; EC 31.4.4) in castor bean (Ricinus communis L.) tissues was investigated by subcellular fractionation and by immuno-electron microscopy. Centrifugal fractionation revealed that most PLD in young leaves was soluble, whereas in mature leaves a majority of PLD was associated with microsomal membranes. Further separation of microsomal membranes by a two-phase partitioning system indicated that PLD was associated with both plasma and intracellular membranes. Sucrose gradient separation of intracellular membranes showed PLD present in the endoplasmic reticulum, a submicrosomal band, and in soluble fractions but not in mitochondria and glyoxysomes of postgermination endosperm. Immunocytochemical studies found high gold labeling in vacuoles in young leaves, suggesting that the high level of soluble PLD in young leaves is due to release of PLD from vacuoles during tissue disruption. In addition to the labeling in vacuoles, gold particles were also found in the cytoplasmic matrices and plasma membrane in leaves and in 2-d postgermination seedlings. Collectively, these results show that PLD in castor bean leaf and seedling tissues is localized in the vacuole and is associated with the endoplasmic reticulum and plasma membrane and that the relative distribution between the soluble and membrane compartments changes during castor bean leaf development</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.111.1.101</identifier><identifier>PMID: 12226277</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>Antibodies ; Biochemistry and Enzymology ; Biological and medical sciences ; Cell membranes ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; Enzymes ; ESTERASAS ; ESTERASE ; ESTRUCTURA CELULAR ; ETAPAS DE DESARROLLO ; FEUILLE ; Fractionation ; Fundamental and applied biological sciences. Psychology ; HOJAS ; Hypocotyls ; IMMUNOLOGIE ; INMUNOLOGIA ; Intracellular membranes ; Lipids ; Metabolism ; Mitochondria ; Plant physiology and development ; Plants ; PLANTULAS ; PLANTULE ; RETICULO ENDOPLASMATICO ; RETICULUM ENDOPLASMIQUE ; RICINUS COMMUNIS ; STADE DE DEVELOPPEMENT ; STRUCTURE CELLULAIRE ; ULTRAESTRUCTURA ; ULTRASTRUCTURE ; VACUOLA ; VACUOLE ; Vacuoles</subject><ispartof>Plant physiology (Bethesda), 1996-05, Vol.111 (1), p.101-107</ispartof><rights>Copyright 1996 American Society of Plant Physiologists</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-1a5b493c624d665a14fdfa10bd57685605347e536f22bcf46a567b031b309eeb3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4277141$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4277141$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,780,784,803,885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3089246$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12226277$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, L. (Kansas State University, Manhattan, KS.)</creatorcontrib><creatorcontrib>Paulsen, A.Q</creatorcontrib><creatorcontrib>Ryu, S.B</creatorcontrib><creatorcontrib>Wang, X</creatorcontrib><title>Intracellular localization of phospholipase D in leaves and seedling tissues of castor bean</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>The intracellular distribution of phospholipase D (PLD; EC 31.4.4) in castor bean (Ricinus communis L.) tissues was investigated by subcellular fractionation and by immuno-electron microscopy. Centrifugal fractionation revealed that most PLD in young leaves was soluble, whereas in mature leaves a majority of PLD was associated with microsomal membranes. Further separation of microsomal membranes by a two-phase partitioning system indicated that PLD was associated with both plasma and intracellular membranes. Sucrose gradient separation of intracellular membranes showed PLD present in the endoplasmic reticulum, a submicrosomal band, and in soluble fractions but not in mitochondria and glyoxysomes of postgermination endosperm. Immunocytochemical studies found high gold labeling in vacuoles in young leaves, suggesting that the high level of soluble PLD in young leaves is due to release of PLD from vacuoles during tissue disruption. In addition to the labeling in vacuoles, gold particles were also found in the cytoplasmic matrices and plasma membrane in leaves and in 2-d postgermination seedlings. Collectively, these results show that PLD in castor bean leaf and seedling tissues is localized in the vacuole and is associated with the endoplasmic reticulum and plasma membrane and that the relative distribution between the soluble and membrane compartments changes during castor bean leaf development</description><subject>Antibodies</subject><subject>Biochemistry and Enzymology</subject><subject>Biological and medical sciences</subject><subject>Cell membranes</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>Enzymes</subject><subject>ESTERASAS</subject><subject>ESTERASE</subject><subject>ESTRUCTURA CELULAR</subject><subject>ETAPAS DE DESARROLLO</subject><subject>FEUILLE</subject><subject>Fractionation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HOJAS</subject><subject>Hypocotyls</subject><subject>IMMUNOLOGIE</subject><subject>INMUNOLOGIA</subject><subject>Intracellular membranes</subject><subject>Lipids</subject><subject>Metabolism</subject><subject>Mitochondria</subject><subject>Plant physiology and development</subject><subject>Plants</subject><subject>PLANTULAS</subject><subject>PLANTULE</subject><subject>RETICULO ENDOPLASMATICO</subject><subject>RETICULUM ENDOPLASMIQUE</subject><subject>RICINUS COMMUNIS</subject><subject>STADE DE DEVELOPPEMENT</subject><subject>STRUCTURE CELLULAIRE</subject><subject>ULTRAESTRUCTURA</subject><subject>ULTRASTRUCTURE</subject><subject>VACUOLA</subject><subject>VACUOLE</subject><subject>Vacuoles</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNpVUU2LFDEQDaK44-jNk4jk4MGDs6by1d0HD7J-LSx40D15CNXdyWyWTNKb9CzorzfNDKNShAp579XHCyHPgZ0DMPlummqG8xoMHpAVKME3XMn2IVkxVu-sbbsz8qSUW8YYCJCPyRlwzjVvmhX5eRnnjIMNYR8w05AGDP43zj5FmhydblKpJ_gJi6UfqY80WLy3hWIcabF2DD5u6exL2dfHqhiwzCnT3mJ8Sh45DMU-O-Y1uf786cfF183Vty-XFx-uNoNs9bwBVL3sxKC5HLVWCNKNDoH1o2p0qzRTQjZWCe047wcnNSrd9ExAL1hnbS_W5P2h7rTvd3Yc7LJSMFP2O8y_TEJv_keivzHbdG9ANS3oqn9z1Od0V9eYzc6XxRKMNu2LgVZ1otFMqEp9e6AOOZWSrTt1AWaW7zDTVDOYGtXsNXn172R_yUf_K-H1kYClOu8yxsGXE0-wtuNymfDlgXa7mHuCZS0Bcmnz4gA7TAa3uVa4_t5VqGNS_AFyR6Vf</recordid><startdate>19960501</startdate><enddate>19960501</enddate><creator>Xu, L. (Kansas State University, Manhattan, KS.)</creator><creator>Paulsen, A.Q</creator><creator>Ryu, S.B</creator><creator>Wang, X</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19960501</creationdate><title>Intracellular localization of phospholipase D in leaves and seedling tissues of castor bean</title><author>Xu, L. (Kansas State University, Manhattan, KS.) ; Paulsen, A.Q ; Ryu, S.B ; Wang, X</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c486t-1a5b493c624d665a14fdfa10bd57685605347e536f22bcf46a567b031b309eeb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Antibodies</topic><topic>Biochemistry and Enzymology</topic><topic>Biological and medical sciences</topic><topic>Cell membranes</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>Enzymes</topic><topic>ESTERASAS</topic><topic>ESTERASE</topic><topic>ESTRUCTURA CELULAR</topic><topic>ETAPAS DE DESARROLLO</topic><topic>FEUILLE</topic><topic>Fractionation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HOJAS</topic><topic>Hypocotyls</topic><topic>IMMUNOLOGIE</topic><topic>INMUNOLOGIA</topic><topic>Intracellular membranes</topic><topic>Lipids</topic><topic>Metabolism</topic><topic>Mitochondria</topic><topic>Plant physiology and development</topic><topic>Plants</topic><topic>PLANTULAS</topic><topic>PLANTULE</topic><topic>RETICULO ENDOPLASMATICO</topic><topic>RETICULUM ENDOPLASMIQUE</topic><topic>RICINUS COMMUNIS</topic><topic>STADE DE DEVELOPPEMENT</topic><topic>STRUCTURE CELLULAIRE</topic><topic>ULTRAESTRUCTURA</topic><topic>ULTRASTRUCTURE</topic><topic>VACUOLA</topic><topic>VACUOLE</topic><topic>Vacuoles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, L. (Kansas State University, Manhattan, KS.)</creatorcontrib><creatorcontrib>Paulsen, A.Q</creatorcontrib><creatorcontrib>Ryu, S.B</creatorcontrib><creatorcontrib>Wang, X</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, L. (Kansas State University, Manhattan, KS.)</au><au>Paulsen, A.Q</au><au>Ryu, S.B</au><au>Wang, X</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intracellular localization of phospholipase D in leaves and seedling tissues of castor bean</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1996-05-01</date><risdate>1996</risdate><volume>111</volume><issue>1</issue><spage>101</spage><epage>107</epage><pages>101-107</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>The intracellular distribution of phospholipase D (PLD; EC 31.4.4) in castor bean (Ricinus communis L.) tissues was investigated by subcellular fractionation and by immuno-electron microscopy. Centrifugal fractionation revealed that most PLD in young leaves was soluble, whereas in mature leaves a majority of PLD was associated with microsomal membranes. Further separation of microsomal membranes by a two-phase partitioning system indicated that PLD was associated with both plasma and intracellular membranes. Sucrose gradient separation of intracellular membranes showed PLD present in the endoplasmic reticulum, a submicrosomal band, and in soluble fractions but not in mitochondria and glyoxysomes of postgermination endosperm. Immunocytochemical studies found high gold labeling in vacuoles in young leaves, suggesting that the high level of soluble PLD in young leaves is due to release of PLD from vacuoles during tissue disruption. In addition to the labeling in vacuoles, gold particles were also found in the cytoplasmic matrices and plasma membrane in leaves and in 2-d postgermination seedlings. Collectively, these results show that PLD in castor bean leaf and seedling tissues is localized in the vacuole and is associated with the endoplasmic reticulum and plasma membrane and that the relative distribution between the soluble and membrane compartments changes during castor bean leaf development</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>12226277</pmid><doi>10.1104/pp.111.1.101</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant physiology (Bethesda), 1996-05, Vol.111 (1), p.101-107 |
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| source | Oxford University Press Journals Current; Free E-Journal (出版社公開部分のみ）; JSTOR |
| subjects | Antibodies Biochemistry and Enzymology Biological and medical sciences Cell membranes COMPOSICION QUIMICA COMPOSITION CHIMIQUE Enzymes ESTERASAS ESTERASE ESTRUCTURA CELULAR ETAPAS DE DESARROLLO FEUILLE Fractionation Fundamental and applied biological sciences. Psychology HOJAS Hypocotyls IMMUNOLOGIE INMUNOLOGIA Intracellular membranes Lipids Metabolism Mitochondria Plant physiology and development Plants PLANTULAS PLANTULE RETICULO ENDOPLASMATICO RETICULUM ENDOPLASMIQUE RICINUS COMMUNIS STADE DE DEVELOPPEMENT STRUCTURE CELLULAIRE ULTRAESTRUCTURA ULTRASTRUCTURE VACUOLA VACUOLE Vacuoles |
| title | Intracellular localization of phospholipase D in leaves and seedling tissues of castor bean |
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