Quantitation of anti-RNP and anti-Sm antibodies in MCTD and SLE patients by immunoblotting
A quantitative immunoblotting assay (QIBA) for the determination of specific antibody titres in human autoimmune sera is described. In this assay, a total HeLa nuclear protein fraction, immobilized on nitrocellulose blot strips, was used as source of antigens and immunoreactive species of autoantibo...
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| Veröffentlicht in: | Clinical and experimental immunology 1985-02, Vol.59 (2), p.457-466 |
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| container_title | Clinical and experimental immunology |
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| creator | HABETS, W. J DE ROOIJ, D. J HOET, M. H VAN DE PUTTE, L. B VAN VENROOIJ, W. J |
| description | A quantitative immunoblotting assay (QIBA) for the determination of specific antibody titres in human autoimmune sera is described. In this assay, a total HeLa nuclear protein fraction, immobilized on nitrocellulose blot strips, was used as source of antigens and immunoreactive species of autoantibodies were quantitated by an enzyme linked second antibody procedure. Besides being more discriminative, QIBA appeared to be up to 500 times more sensitive than immunodiffusion or immunoelectrophoresis. In this study we used 21 sera from patients with SLE or MCTD for a quantitative analysis of their specific autoantibody content. Within this group, a very diverse spectrum of antibody populations was observed; anti-RNP sera appeared to contain, among others, high tired antibody versus 70K and 31K polypeptides while all (n = 6) anti-Sm sera recognized a 25kD protein doublet. In a follow-up study of two MCTD patients significant flares in specific antibody content could be observed. |
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J ; DE ROOIJ, D. J ; HOET, M. H ; VAN DE PUTTE, L. B ; VAN VENROOIJ, W. J</creator><creatorcontrib>HABETS, W. J ; DE ROOIJ, D. J ; HOET, M. H ; VAN DE PUTTE, L. B ; VAN VENROOIJ, W. J</creatorcontrib><description>A quantitative immunoblotting assay (QIBA) for the determination of specific antibody titres in human autoimmune sera is described. In this assay, a total HeLa nuclear protein fraction, immobilized on nitrocellulose blot strips, was used as source of antigens and immunoreactive species of autoantibodies were quantitated by an enzyme linked second antibody procedure. Besides being more discriminative, QIBA appeared to be up to 500 times more sensitive than immunodiffusion or immunoelectrophoresis. In this study we used 21 sera from patients with SLE or MCTD for a quantitative analysis of their specific autoantibody content. Within this group, a very diverse spectrum of antibody populations was observed; anti-RNP sera appeared to contain, among others, high tired antibody versus 70K and 31K polypeptides while all (n = 6) anti-Sm sera recognized a 25kD protein doublet. In a follow-up study of two MCTD patients significant flares in specific antibody content could be observed.</description><identifier>ISSN: 0009-9104</identifier><identifier>EISSN: 1365-2249</identifier><identifier>PMID: 3884199</identifier><identifier>CODEN: CEXIAL</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Adult ; Aged ; Antibodies, Antinuclear - analysis ; Antibody Specificity ; Antigens - immunology ; Autoantigens ; Biological and medical sciences ; Collodion ; Dose-Response Relationship, Immunologic ; Female ; Follow-Up Studies ; Humans ; Immunologic Techniques ; Lupus Erythematosus, Systemic - immunology ; Medical sciences ; Mixed Connective Tissue Disease - immunology ; Ribonucleoproteins - immunology ; Ribonucleoproteins, Small Nuclear ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. 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J</creatorcontrib><creatorcontrib>DE ROOIJ, D. J</creatorcontrib><creatorcontrib>HOET, M. H</creatorcontrib><creatorcontrib>VAN DE PUTTE, L. B</creatorcontrib><creatorcontrib>VAN VENROOIJ, W. J</creatorcontrib><title>Quantitation of anti-RNP and anti-Sm antibodies in MCTD and SLE patients by immunoblotting</title><title>Clinical and experimental immunology</title><addtitle>Clin Exp Immunol</addtitle><description>A quantitative immunoblotting assay (QIBA) for the determination of specific antibody titres in human autoimmune sera is described. In this assay, a total HeLa nuclear protein fraction, immobilized on nitrocellulose blot strips, was used as source of antigens and immunoreactive species of autoantibodies were quantitated by an enzyme linked second antibody procedure. Besides being more discriminative, QIBA appeared to be up to 500 times more sensitive than immunodiffusion or immunoelectrophoresis. In this study we used 21 sera from patients with SLE or MCTD for a quantitative analysis of their specific autoantibody content. Within this group, a very diverse spectrum of antibody populations was observed; anti-RNP sera appeared to contain, among others, high tired antibody versus 70K and 31K polypeptides while all (n = 6) anti-Sm sera recognized a 25kD protein doublet. In a follow-up study of two MCTD patients significant flares in specific antibody content could be observed.</description><subject>Adult</subject><subject>Aged</subject><subject>Antibodies, Antinuclear - analysis</subject><subject>Antibody Specificity</subject><subject>Antigens - immunology</subject><subject>Autoantigens</subject><subject>Biological and medical sciences</subject><subject>Collodion</subject><subject>Dose-Response Relationship, Immunologic</subject><subject>Female</subject><subject>Follow-Up Studies</subject><subject>Humans</subject><subject>Immunologic Techniques</subject><subject>Lupus Erythematosus, Systemic - immunology</subject><subject>Medical sciences</subject><subject>Mixed Connective Tissue Disease - immunology</subject><subject>Ribonucleoproteins - immunology</subject><subject>Ribonucleoproteins, Small Nuclear</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><subject>snRNP Core Proteins</subject><issn>0009-9104</issn><issn>1365-2249</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUMtOwzAQtBColMInIOWAuEXyM4kvSKi0gFSeLRcukeM4xSixQ-wg9e8xbVTBZXdHszuzuwdgjEjCYowpPwRjCCGPOYL0GJw49xlgkiR4BEYkyyjifAzeX3phvPbCa2siW0W_KH59fA5FuQPLZpsLW2rlIm2ih-nqZksvF7OoDZPKeBcVm0g3TW9sUVvvtVmfgqNK1E6dDXkC3uaz1fQuXjzd3k-vF3GLOfQxIUQpyGCFJRcorEgxTRmXkiLEMpVUrCRCVSTEEhPOypKkMqWJQBxJiCmZgKudbtsXjSpl2KYTdd52uhHdJrdC5_8Zoz_ytf3OEUtTxGAQuBwEOvvVK-fzRjup6loYZXuXpwkkGEMWGs__Ou0thm8G_mLghZOirjphpHb7toyG23BKfgBVXn6L</recordid><startdate>19850201</startdate><enddate>19850201</enddate><creator>HABETS, W. J</creator><creator>DE ROOIJ, D. J</creator><creator>HOET, M. H</creator><creator>VAN DE PUTTE, L. B</creator><creator>VAN VENROOIJ, W. 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J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p290t-333ee050f2c9a1000424759cc41158e6f5d3aef3d3ad2395dd37c746a191c0243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Antibodies, Antinuclear - analysis</topic><topic>Antibody Specificity</topic><topic>Antigens - immunology</topic><topic>Autoantigens</topic><topic>Biological and medical sciences</topic><topic>Collodion</topic><topic>Dose-Response Relationship, Immunologic</topic><topic>Female</topic><topic>Follow-Up Studies</topic><topic>Humans</topic><topic>Immunologic Techniques</topic><topic>Lupus Erythematosus, Systemic - immunology</topic><topic>Medical sciences</topic><topic>Mixed Connective Tissue Disease - immunology</topic><topic>Ribonucleoproteins - immunology</topic><topic>Ribonucleoproteins, Small Nuclear</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><topic>snRNP Core Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HABETS, W. J</creatorcontrib><creatorcontrib>DE ROOIJ, D. J</creatorcontrib><creatorcontrib>HOET, M. H</creatorcontrib><creatorcontrib>VAN DE PUTTE, L. B</creatorcontrib><creatorcontrib>VAN VENROOIJ, W. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Clinical and experimental immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HABETS, W. J</au><au>DE ROOIJ, D. J</au><au>HOET, M. H</au><au>VAN DE PUTTE, L. B</au><au>VAN VENROOIJ, W. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitation of anti-RNP and anti-Sm antibodies in MCTD and SLE patients by immunoblotting</atitle><jtitle>Clinical and experimental immunology</jtitle><addtitle>Clin Exp Immunol</addtitle><date>1985-02-01</date><risdate>1985</risdate><volume>59</volume><issue>2</issue><spage>457</spage><epage>466</epage><pages>457-466</pages><issn>0009-9104</issn><eissn>1365-2249</eissn><coden>CEXIAL</coden><abstract>A quantitative immunoblotting assay (QIBA) for the determination of specific antibody titres in human autoimmune sera is described. In this assay, a total HeLa nuclear protein fraction, immobilized on nitrocellulose blot strips, was used as source of antigens and immunoreactive species of autoantibodies were quantitated by an enzyme linked second antibody procedure. Besides being more discriminative, QIBA appeared to be up to 500 times more sensitive than immunodiffusion or immunoelectrophoresis. In this study we used 21 sera from patients with SLE or MCTD for a quantitative analysis of their specific autoantibody content. Within this group, a very diverse spectrum of antibody populations was observed; anti-RNP sera appeared to contain, among others, high tired antibody versus 70K and 31K polypeptides while all (n = 6) anti-Sm sera recognized a 25kD protein doublet. In a follow-up study of two MCTD patients significant flares in specific antibody content could be observed.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>3884199</pmid><tpages>10</tpages></addata></record> |
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| ispartof | Clinical and experimental immunology, 1985-02, Vol.59 (2), p.457-466 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Adult Aged Antibodies, Antinuclear - analysis Antibody Specificity Antigens - immunology Autoantigens Biological and medical sciences Collodion Dose-Response Relationship, Immunologic Female Follow-Up Studies Humans Immunologic Techniques Lupus Erythematosus, Systemic - immunology Medical sciences Mixed Connective Tissue Disease - immunology Ribonucleoproteins - immunology Ribonucleoproteins, Small Nuclear Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis snRNP Core Proteins |
| title | Quantitation of anti-RNP and anti-Sm antibodies in MCTD and SLE patients by immunoblotting |
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