Characterization of an endo-beta-1,4-glucanase gene induced by auxin in elongating pea epicotyls
A gene (EGL1) encoding an endo-beta-1,4-D-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has been cloned and characterized. EGL1 encodes a 486-amino acid polypeptide, including a 24-mer putative signal peptide. The mature protein has a calculated molecular mass of 51.3 kD and an isoelectric po...
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description | A gene (EGL1) encoding an endo-beta-1,4-D-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has been cloned and characterized. EGL1 encodes a 486-amino acid polypeptide, including a 24-mer putative signal peptide. The mature protein has a calculated molecular mass of 51.3 kD and an isoelectric point of 9.1. This pea EGase shares significant similarity with EGases from other plant species, but it appears to be distinct from the EGases associated with abscission and fruit ripening. Although EGL1 transcripts are detected in all parts of pea plants, they are relatively abundant in flowers and young pods undergoing rapid growth and most abundant in elongating epicotyls of etiolated seedlings. When epicotyl segments (6 mm long, 4 mm from the apical hook) are incubated in a 5 micromolar solution of the synthetic auxin analog 2,4-dichlorophenoxyacetic acid, the concentration of EGL1 mRNA increases about 10-fold when the segments elongate most rapidly |
doi_str_mv | 10.1104/pp.110.1.163 |
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(University of Georgia, Athens, GA.) ; Blumer, J.M ; Darvill, A.G ; Albersheim, P</creator><creatorcontrib>Wu, S.C. (University of Georgia, Athens, GA.) ; Blumer, J.M ; Darvill, A.G ; Albersheim, P</creatorcontrib><description>A gene (EGL1) encoding an endo-beta-1,4-D-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has been cloned and characterized. EGL1 encodes a 486-amino acid polypeptide, including a 24-mer putative signal peptide. The mature protein has a calculated molecular mass of 51.3 kD and an isoelectric point of 9.1. This pea EGase shares significant similarity with EGases from other plant species, but it appears to be distinct from the EGases associated with abscission and fruit ripening. Although EGL1 transcripts are detected in all parts of pea plants, they are relatively abundant in flowers and young pods undergoing rapid growth and most abundant in elongating epicotyls of etiolated seedlings. When epicotyl segments (6 mm long, 4 mm from the apical hook) are incubated in a 5 micromolar solution of the synthetic auxin analog 2,4-dichlorophenoxyacetic acid, the concentration of EGL1 mRNA increases about 10-fold when the segments elongate most rapidly</description><identifier>ISSN: 0032-0889</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.110.1.163</identifier><identifier>PMID: 8587980</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Physiologists</publisher><subject>2,4-D ; 2,4-Dichlorophenoxyacetic Acid ; 2,4-Dichlorophenoxyacetic Acid - pharmacology ; Abscission ; ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Amino Acid Sequence ; amino acid sequences ; Base Sequence ; beta-glucanase ; Biological and medical sciences ; cell growth ; Cell walls ; Cellulase ; Cellulase - genetics ; CELLULE ; CELULAS ; Cloning, Molecular ; CODE GENETIQUE ; CODIGO GENETICO ; Complementary DNA ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; CRECIMIENTO ; CROISSANCE ; DNA, Complementary ; DNA, Complementary - genetics ; drug effects ; enzyme activity ; Enzymes ; enzymology ; EPICOTILOS ; EPICOTYLE ; Epicotyls ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE ; gene expression ; Gene Expression Regulation, Plant ; Gene Regulation and Molecular Genetics ; GENES ; Genes, Plant ; Genes. Genome ; genetic code ; genetics ; GLICOSIDASAS ; GLYCOSIDASE ; Indoleacetic Acids ; Indoleacetic Acids - pharmacology ; Messenger RNA ; Metabolism ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; nucleotide sequences ; Peas ; pharmacology ; PISUM SATIVUM ; Pisum sativum - enzymology ; Pisum sativum - genetics ; Plant physiology and development ; Plant Proteins ; Plant Proteins - genetics ; Plant Shoots ; Plant Shoots - drug effects ; Plants ; Polymerase chain reaction ; Ripening ; RNA ; RNA, Messenger ; RNA, Messenger - genetics ; RNA, Plant ; RNA, Plant - genetics ; SECUENCIA NUCLEOTIDICA ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; SEQUENCE NUCLEOTIDIQUE ; Tissue Distribution</subject><ispartof>Plant physiology (Bethesda), 1996-01, Vol.110 (1), p.163-170</ispartof><rights>Copyright 1996 American Society of Plant Physiologists</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/4276976$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/4276976$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,776,780,799,881,4010,27900,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2984103$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8587980$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, S.C. (University of Georgia, Athens, GA.)</creatorcontrib><creatorcontrib>Blumer, J.M</creatorcontrib><creatorcontrib>Darvill, A.G</creatorcontrib><creatorcontrib>Albersheim, P</creatorcontrib><title>Characterization of an endo-beta-1,4-glucanase gene induced by auxin in elongating pea epicotyls</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>A gene (EGL1) encoding an endo-beta-1,4-D-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has been cloned and characterized. EGL1 encodes a 486-amino acid polypeptide, including a 24-mer putative signal peptide. The mature protein has a calculated molecular mass of 51.3 kD and an isoelectric point of 9.1. This pea EGase shares significant similarity with EGases from other plant species, but it appears to be distinct from the EGases associated with abscission and fruit ripening. Although EGL1 transcripts are detected in all parts of pea plants, they are relatively abundant in flowers and young pods undergoing rapid growth and most abundant in elongating epicotyls of etiolated seedlings. When epicotyl segments (6 mm long, 4 mm from the apical hook) are incubated in a 5 micromolar solution of the synthetic auxin analog 2,4-dichlorophenoxyacetic acid, the concentration of EGL1 mRNA increases about 10-fold when the segments elongate most rapidly</description><subject>2,4-D</subject><subject>2,4-Dichlorophenoxyacetic Acid</subject><subject>2,4-Dichlorophenoxyacetic Acid - pharmacology</subject><subject>Abscission</subject><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Base Sequence</subject><subject>beta-glucanase</subject><subject>Biological and medical sciences</subject><subject>cell growth</subject><subject>Cell walls</subject><subject>Cellulase</subject><subject>Cellulase - genetics</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>Cloning, Molecular</subject><subject>CODE GENETIQUE</subject><subject>CODIGO GENETICO</subject><subject>Complementary DNA</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>CRECIMIENTO</subject><subject>CROISSANCE</subject><subject>DNA, Complementary</subject><subject>DNA, Complementary - genetics</subject><subject>drug effects</subject><subject>enzyme activity</subject><subject>Enzymes</subject><subject>enzymology</subject><subject>EPICOTILOS</subject><subject>EPICOTYLE</subject><subject>Epicotyls</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Gene Regulation and Molecular Genetics</subject><subject>GENES</subject><subject>Genes, Plant</subject><subject>Genes. Genome</subject><subject>genetic code</subject><subject>genetics</subject><subject>GLICOSIDASAS</subject><subject>GLYCOSIDASE</subject><subject>Indoleacetic Acids</subject><subject>Indoleacetic Acids - pharmacology</subject><subject>Messenger RNA</subject><subject>Metabolism</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Peas</subject><subject>pharmacology</subject><subject>PISUM SATIVUM</subject><subject>Pisum sativum - enzymology</subject><subject>Pisum sativum - genetics</subject><subject>Plant physiology and development</subject><subject>Plant Proteins</subject><subject>Plant Proteins - genetics</subject><subject>Plant Shoots</subject><subject>Plant Shoots - drug effects</subject><subject>Plants</subject><subject>Polymerase chain reaction</subject><subject>Ripening</subject><subject>RNA</subject><subject>RNA, Messenger</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Plant</subject><subject>RNA, Plant - genetics</subject><subject>SECUENCIA NUCLEOTIDICA</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>SEQUENCE NUCLEOTIDIQUE</subject><subject>Tissue Distribution</subject><issn>0032-0889</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD2P1DAQhi0EOpaDjgqB5AJRkcWfsV1cgVZ8SSdRwNVh4kxyPmXtECeI5ddjtKsVVFQzmueZV6Mh5ClnW86ZejNNf-qWb3kt75EN11JUQit7n2wYKz2z1j0kj3K-Y4xxydUFubDaGmfZhnzb3cIMfsE5_IIlpEhTTyFSjF2qWlyg4q9VNYyrhwgZ6YARaYjd6rGj7YHC-jPEMqA4pjiUhDjQCYHiFHxaDmN-TB70MGZ8cqqX5Ob9u6-7j9X15w-fdm-vq146vlQepTUg-topLzrmudLCcM-N0Nx1qCUzRrQgC3ctWFY76Wpkuu9kr7wX8pJcHXOntd1j5zEuM4zNNIc9zIcmQWj-JTHcNkP60XBtDNNl_9Vpf07fV8xLsw_Z4zhCxLTmRlkrpTbuvyKvFauZNUV88fdF51NOvy_85YlD9jD2M0Qf8lkTzirOZNGeH7W7vKT5jJUwtTN1wc-OuIfUwDCXhJsvznBltZC_AZVjpEc</recordid><startdate>199601</startdate><enddate>199601</enddate><creator>Wu, S.C. (University of Georgia, Athens, GA.)</creator><creator>Blumer, J.M</creator><creator>Darvill, A.G</creator><creator>Albersheim, P</creator><general>American Society of Plant Physiologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope></search><sort><creationdate>199601</creationdate><title>Characterization of an endo-beta-1,4-glucanase gene induced by auxin in elongating pea epicotyls</title><author>Wu, S.C. (University of Georgia, Athens, GA.) ; Blumer, J.M ; Darvill, A.G ; Albersheim, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f391t-ce387a2f694c2d0c145271c172519de530772ba36949ba8069396e05fd3f4cc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>2,4-D</topic><topic>2,4-Dichlorophenoxyacetic Acid</topic><topic>2,4-Dichlorophenoxyacetic Acid - pharmacology</topic><topic>Abscission</topic><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Base Sequence</topic><topic>beta-glucanase</topic><topic>Biological and medical sciences</topic><topic>cell growth</topic><topic>Cell walls</topic><topic>Cellulase</topic><topic>Cellulase - genetics</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>Cloning, Molecular</topic><topic>CODE GENETIQUE</topic><topic>CODIGO GENETICO</topic><topic>Complementary DNA</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>CRECIMIENTO</topic><topic>CROISSANCE</topic><topic>DNA, Complementary</topic><topic>DNA, Complementary - genetics</topic><topic>drug effects</topic><topic>enzyme activity</topic><topic>Enzymes</topic><topic>enzymology</topic><topic>EPICOTILOS</topic><topic>EPICOTYLE</topic><topic>Epicotyls</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Gene Regulation and Molecular Genetics</topic><topic>GENES</topic><topic>Genes, Plant</topic><topic>Genes. Genome</topic><topic>genetic code</topic><topic>genetics</topic><topic>GLICOSIDASAS</topic><topic>GLYCOSIDASE</topic><topic>Indoleacetic Acids</topic><topic>Indoleacetic Acids - pharmacology</topic><topic>Messenger RNA</topic><topic>Metabolism</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Peas</topic><topic>pharmacology</topic><topic>PISUM SATIVUM</topic><topic>Pisum sativum - enzymology</topic><topic>Pisum sativum - genetics</topic><topic>Plant physiology and development</topic><topic>Plant Proteins</topic><topic>Plant Proteins - genetics</topic><topic>Plant Shoots</topic><topic>Plant Shoots - drug effects</topic><topic>Plants</topic><topic>Polymerase chain reaction</topic><topic>Ripening</topic><topic>RNA</topic><topic>RNA, Messenger</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Plant</topic><topic>RNA, Plant - genetics</topic><topic>SECUENCIA NUCLEOTIDICA</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>SEQUENCE NUCLEOTIDIQUE</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, S.C. (University of Georgia, Athens, GA.)</creatorcontrib><creatorcontrib>Blumer, J.M</creatorcontrib><creatorcontrib>Darvill, A.G</creatorcontrib><creatorcontrib>Albersheim, P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, S.C. (University of Georgia, Athens, GA.)</au><au>Blumer, J.M</au><au>Darvill, A.G</au><au>Albersheim, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of an endo-beta-1,4-glucanase gene induced by auxin in elongating pea epicotyls</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>1996-01</date><risdate>1996</risdate><volume>110</volume><issue>1</issue><spage>163</spage><epage>170</epage><pages>163-170</pages><issn>0032-0889</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>A gene (EGL1) encoding an endo-beta-1,4-D-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has been cloned and characterized. EGL1 encodes a 486-amino acid polypeptide, including a 24-mer putative signal peptide. The mature protein has a calculated molecular mass of 51.3 kD and an isoelectric point of 9.1. This pea EGase shares significant similarity with EGases from other plant species, but it appears to be distinct from the EGases associated with abscission and fruit ripening. Although EGL1 transcripts are detected in all parts of pea plants, they are relatively abundant in flowers and young pods undergoing rapid growth and most abundant in elongating epicotyls of etiolated seedlings. When epicotyl segments (6 mm long, 4 mm from the apical hook) are incubated in a 5 micromolar solution of the synthetic auxin analog 2,4-dichlorophenoxyacetic acid, the concentration of EGL1 mRNA increases about 10-fold when the segments elongate most rapidly</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Physiologists</pub><pmid>8587980</pmid><doi>10.1104/pp.110.1.163</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
subjects | 2,4-D 2,4-Dichlorophenoxyacetic Acid 2,4-Dichlorophenoxyacetic Acid - pharmacology Abscission ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Amino Acid Sequence amino acid sequences Base Sequence beta-glucanase Biological and medical sciences cell growth Cell walls Cellulase Cellulase - genetics CELLULE CELULAS Cloning, Molecular CODE GENETIQUE CODIGO GENETICO Complementary DNA COMPOSICION QUIMICA COMPOSITION CHIMIQUE CRECIMIENTO CROISSANCE DNA, Complementary DNA, Complementary - genetics drug effects enzyme activity Enzymes enzymology EPICOTILOS EPICOTYLE Epicotyls EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE gene expression Gene Expression Regulation, Plant Gene Regulation and Molecular Genetics GENES Genes, Plant Genes. Genome genetic code genetics GLICOSIDASAS GLYCOSIDASE Indoleacetic Acids Indoleacetic Acids - pharmacology Messenger RNA Metabolism Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequences Peas pharmacology PISUM SATIVUM Pisum sativum - enzymology Pisum sativum - genetics Plant physiology and development Plant Proteins Plant Proteins - genetics Plant Shoots Plant Shoots - drug effects Plants Polymerase chain reaction Ripening RNA RNA, Messenger RNA, Messenger - genetics RNA, Plant RNA, Plant - genetics SECUENCIA NUCLEOTIDICA Sequence Analysis, DNA Sequence Homology, Amino Acid SEQUENCE NUCLEOTIDIQUE Tissue Distribution |
title | Characterization of an endo-beta-1,4-glucanase gene induced by auxin in elongating pea epicotyls |
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