Correlation between spontaneous electrical, calcium and mechanical activity in detrusor smooth muscle of the guinea‐pig bladder
To investigate the cellular mechanisms underlying spontaneous excitation of smooth muscle of the guinea‐pig urinary bladder, isometric tension was measured in muscle bundles while recording the membrane potential from a cell in the bundle with a microeletrode. Changes in the intracellular calcium co...
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description | To investigate the cellular mechanisms underlying spontaneous excitation of smooth muscle of the guinea‐pig urinary bladder, isometric tension was measured in muscle bundles while recording the membrane potential from a cell in the bundle with a microeletrode. Changes in the intracellular calcium concentration ([Ca2+]i; calcium transients) were recorded in strips loaded with the fluorescent dye, fura‐PE3.
In 40% of preparations, individual action potentials and contractions, which were abolished by nifedipine (1 μM), were generated. In the remaining preparations, bursting action potentials and contractions were generated. Contractions were again abolished by nifedipine (1 μM), while higher concentrations of nifedipine (10–30 μM) were required to prevent the electrical activity.
Carbachol (0.1 μM) increased the frequency of action potentials and corresponding contractions. Apamin (0.1 μM) potentiated bursting activity and enhanced phasic contraction. Charybdotoxin (CTX, 50 nM) induced prolonged action potentials that generated enlarged contractions. In contrast, levcromakalim (0.1 μM) reduced the frequency of action potentials, action potential bursts and the size of the contractions.
Forskolin (0.1 μM), 8‐bromoguanosin 3′, 5′ cyclic monophosphate (8Br‐cGMP, 0.1 mM) and Y‐26763 (10 μM) suppressed contractions without reducing the amplitude of either action potentials or Ca transients.
This paper confirms that action potentials and associated calcium transients are fundamental mechanisms in generating spontaneous contractions in smooth muscles of the guinea‐pig bladder. However, in parallel with the excitation–contraction coupling, the sensitivity of the contractile proteins for Ca2+ may play an important role in regulating spontaneous excitation and can be modulated by cyclic nucleotides and Rho kinase.
British Journal of Pharmacology (2004) 141, 183–193. doi:10.1038/sj.bjp.0705602 |
doi_str_mv | 10.1038/sj.bjp.0705602 |
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In 40% of preparations, individual action potentials and contractions, which were abolished by nifedipine (1 μM), were generated. In the remaining preparations, bursting action potentials and contractions were generated. Contractions were again abolished by nifedipine (1 μM), while higher concentrations of nifedipine (10–30 μM) were required to prevent the electrical activity.
Carbachol (0.1 μM) increased the frequency of action potentials and corresponding contractions. Apamin (0.1 μM) potentiated bursting activity and enhanced phasic contraction. Charybdotoxin (CTX, 50 nM) induced prolonged action potentials that generated enlarged contractions. In contrast, levcromakalim (0.1 μM) reduced the frequency of action potentials, action potential bursts and the size of the contractions.
Forskolin (0.1 μM), 8‐bromoguanosin 3′, 5′ cyclic monophosphate (8Br‐cGMP, 0.1 mM) and Y‐26763 (10 μM) suppressed contractions without reducing the amplitude of either action potentials or Ca transients.
This paper confirms that action potentials and associated calcium transients are fundamental mechanisms in generating spontaneous contractions in smooth muscles of the guinea‐pig bladder. However, in parallel with the excitation–contraction coupling, the sensitivity of the contractile proteins for Ca2+ may play an important role in regulating spontaneous excitation and can be modulated by cyclic nucleotides and Rho kinase.
British Journal of Pharmacology (2004) 141, 183–193. doi:10.1038/sj.bjp.0705602</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1038/sj.bjp.0705602</identifier><identifier>PMID: 14662721</identifier><identifier>CODEN: BJPCBM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>action potential ; Action Potentials - drug effects ; Action Potentials - physiology ; Amides - pharmacology ; Animals ; Apamin - pharmacology ; Biological and medical sciences ; Boron Compounds - pharmacology ; Ca sensitivity ; Calcium - chemistry ; Calcium - physiology ; Calcium Signaling - physiology ; Carbachol - pharmacology ; Charybdotoxin - pharmacology ; Colforsin - pharmacology ; Cromakalim - pharmacology ; Cyclic GMP - analogs & derivatives ; Cyclic GMP - pharmacology ; cyclic nucleotide ; Dose-Response Relationship, Drug ; Drug Synergism ; Female ; Fura-2 ; Guinea Pigs ; Isotonic Contraction - drug effects ; Isotonic Contraction - physiology ; Male ; Mechanotransduction, Cellular - physiology ; Medical sciences ; Microelectrodes ; Muscle Contraction - drug effects ; Muscle, Smooth - drug effects ; Muscle, Smooth - physiology ; Nifedipine - pharmacology ; Pharmacology. Drug treatments ; Pyridines - pharmacology ; Transducers ; Urinary bladder ; Urinary Bladder - cytology ; Urinary Bladder - drug effects ; Urinary Bladder - physiology</subject><ispartof>British journal of pharmacology, 2004-01, Vol.141 (1), p.183-193</ispartof><rights>2004 British Pharmacological Society</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Jan 2004</rights><rights>Copyright 2004, Nature Publishing Group 2004 Nature Publishing Group</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5534-ff43b9ea64fa214c8a78ec44f13512de54efc697195a14d2cb79172b2af4b1ad3</citedby><cites>FETCH-LOGICAL-c5534-ff43b9ea64fa214c8a78ec44f13512de54efc697195a14d2cb79172b2af4b1ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1574183/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1574183/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15656913$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14662721$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hashitani, Hikaru</creatorcontrib><creatorcontrib>Brading, Alison F</creatorcontrib><creatorcontrib>Suzuki, Hikaru</creatorcontrib><title>Correlation between spontaneous electrical, calcium and mechanical activity in detrusor smooth muscle of the guinea‐pig bladder</title><title>British journal of pharmacology</title><addtitle>Br J Pharmacol</addtitle><description>To investigate the cellular mechanisms underlying spontaneous excitation of smooth muscle of the guinea‐pig urinary bladder, isometric tension was measured in muscle bundles while recording the membrane potential from a cell in the bundle with a microeletrode. Changes in the intracellular calcium concentration ([Ca2+]i; calcium transients) were recorded in strips loaded with the fluorescent dye, fura‐PE3.
In 40% of preparations, individual action potentials and contractions, which were abolished by nifedipine (1 μM), were generated. In the remaining preparations, bursting action potentials and contractions were generated. Contractions were again abolished by nifedipine (1 μM), while higher concentrations of nifedipine (10–30 μM) were required to prevent the electrical activity.
Carbachol (0.1 μM) increased the frequency of action potentials and corresponding contractions. Apamin (0.1 μM) potentiated bursting activity and enhanced phasic contraction. Charybdotoxin (CTX, 50 nM) induced prolonged action potentials that generated enlarged contractions. In contrast, levcromakalim (0.1 μM) reduced the frequency of action potentials, action potential bursts and the size of the contractions.
Forskolin (0.1 μM), 8‐bromoguanosin 3′, 5′ cyclic monophosphate (8Br‐cGMP, 0.1 mM) and Y‐26763 (10 μM) suppressed contractions without reducing the amplitude of either action potentials or Ca transients.
This paper confirms that action potentials and associated calcium transients are fundamental mechanisms in generating spontaneous contractions in smooth muscles of the guinea‐pig bladder. However, in parallel with the excitation–contraction coupling, the sensitivity of the contractile proteins for Ca2+ may play an important role in regulating spontaneous excitation and can be modulated by cyclic nucleotides and Rho kinase.
British Journal of Pharmacology (2004) 141, 183–193. doi:10.1038/sj.bjp.0705602</description><subject>action potential</subject><subject>Action Potentials - drug effects</subject><subject>Action Potentials - physiology</subject><subject>Amides - pharmacology</subject><subject>Animals</subject><subject>Apamin - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Boron Compounds - pharmacology</subject><subject>Ca sensitivity</subject><subject>Calcium - chemistry</subject><subject>Calcium - physiology</subject><subject>Calcium Signaling - physiology</subject><subject>Carbachol - pharmacology</subject><subject>Charybdotoxin - pharmacology</subject><subject>Colforsin - pharmacology</subject><subject>Cromakalim - pharmacology</subject><subject>Cyclic GMP - analogs & derivatives</subject><subject>Cyclic GMP - pharmacology</subject><subject>cyclic nucleotide</subject><subject>Dose-Response Relationship, Drug</subject><subject>Drug Synergism</subject><subject>Female</subject><subject>Fura-2</subject><subject>Guinea Pigs</subject><subject>Isotonic Contraction - drug effects</subject><subject>Isotonic Contraction - physiology</subject><subject>Male</subject><subject>Mechanotransduction, Cellular - physiology</subject><subject>Medical sciences</subject><subject>Microelectrodes</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - physiology</subject><subject>Nifedipine - pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Pyridines - pharmacology</subject><subject>Transducers</subject><subject>Urinary bladder</subject><subject>Urinary Bladder - cytology</subject><subject>Urinary Bladder - drug effects</subject><subject>Urinary Bladder - physiology</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUGv1CAUhRuj8Y1Pty4NMXFnR6BQ2o2JTtRn8hJd6JpQepmhaaECfS-z03_gb_SXyGQan67cQML9OOfknqJ4SvCW4Kp5FYdtN8xbLDCvMb1XbAgTdcmrhtwvNhhjURLSNBfFoxgHjPNQ8IfFBWF1TQUlm-LHzocAo0rWO9RBugVwKM7eJeXALxHBCDoFq9X4EuVD22VCyvVoAn1Q7vSOlE72xqYjsg71kMISfUBx8j4d0LREPQLyBqUDoP1iHahf33_Odo-6UfU9hMfFA6PGCE_W-7L4-v7dl91Vef3pw8fdm-tSc16x0hhWdS2omhlFCdONEg1oxgypOKE9cAZG160gLVeE9VR3oiWCdlQZ1hHVV5fF67PuvHQT9BpcCmqUc7CTCkfplZX_Tpw9yL2_kYQLRpoqCzxfBYL_tkBMcvBLcDmzpCT70txHhrZnSAcfYwDzx4BgeWpMxkHmxuTaWP7w7O9Yd_haUQZerICKedsmKKdtvON4zeuWnOJVZ-7WjnD8j618-_mKcc6q31PAtLw</recordid><startdate>200401</startdate><enddate>200401</enddate><creator>Hashitani, Hikaru</creator><creator>Brading, Alison F</creator><creator>Suzuki, Hikaru</creator><general>Blackwell Publishing Ltd</general><general>Nature Publishing</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>200401</creationdate><title>Correlation between spontaneous electrical, calcium and mechanical activity in detrusor smooth muscle of the guinea‐pig bladder</title><author>Hashitani, Hikaru ; Brading, Alison F ; Suzuki, Hikaru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5534-ff43b9ea64fa214c8a78ec44f13512de54efc697195a14d2cb79172b2af4b1ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>action potential</topic><topic>Action Potentials - drug effects</topic><topic>Action Potentials - physiology</topic><topic>Amides - pharmacology</topic><topic>Animals</topic><topic>Apamin - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Boron Compounds - pharmacology</topic><topic>Ca sensitivity</topic><topic>Calcium - chemistry</topic><topic>Calcium - physiology</topic><topic>Calcium Signaling - physiology</topic><topic>Carbachol - pharmacology</topic><topic>Charybdotoxin - pharmacology</topic><topic>Colforsin - pharmacology</topic><topic>Cromakalim - pharmacology</topic><topic>Cyclic GMP - analogs & derivatives</topic><topic>Cyclic GMP - pharmacology</topic><topic>cyclic nucleotide</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Synergism</topic><topic>Female</topic><topic>Fura-2</topic><topic>Guinea Pigs</topic><topic>Isotonic Contraction - drug effects</topic><topic>Isotonic Contraction - physiology</topic><topic>Male</topic><topic>Mechanotransduction, Cellular - physiology</topic><topic>Medical sciences</topic><topic>Microelectrodes</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - physiology</topic><topic>Nifedipine - pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Pyridines - pharmacology</topic><topic>Transducers</topic><topic>Urinary bladder</topic><topic>Urinary Bladder - cytology</topic><topic>Urinary Bladder - drug effects</topic><topic>Urinary Bladder - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hashitani, Hikaru</creatorcontrib><creatorcontrib>Brading, Alison F</creatorcontrib><creatorcontrib>Suzuki, Hikaru</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hashitani, Hikaru</au><au>Brading, Alison F</au><au>Suzuki, Hikaru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Correlation between spontaneous electrical, calcium and mechanical activity in detrusor smooth muscle of the guinea‐pig bladder</atitle><jtitle>British journal of pharmacology</jtitle><addtitle>Br J Pharmacol</addtitle><date>2004-01</date><risdate>2004</risdate><volume>141</volume><issue>1</issue><spage>183</spage><epage>193</epage><pages>183-193</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><coden>BJPCBM</coden><abstract>To investigate the cellular mechanisms underlying spontaneous excitation of smooth muscle of the guinea‐pig urinary bladder, isometric tension was measured in muscle bundles while recording the membrane potential from a cell in the bundle with a microeletrode. Changes in the intracellular calcium concentration ([Ca2+]i; calcium transients) were recorded in strips loaded with the fluorescent dye, fura‐PE3.
In 40% of preparations, individual action potentials and contractions, which were abolished by nifedipine (1 μM), were generated. In the remaining preparations, bursting action potentials and contractions were generated. Contractions were again abolished by nifedipine (1 μM), while higher concentrations of nifedipine (10–30 μM) were required to prevent the electrical activity.
Carbachol (0.1 μM) increased the frequency of action potentials and corresponding contractions. Apamin (0.1 μM) potentiated bursting activity and enhanced phasic contraction. Charybdotoxin (CTX, 50 nM) induced prolonged action potentials that generated enlarged contractions. In contrast, levcromakalim (0.1 μM) reduced the frequency of action potentials, action potential bursts and the size of the contractions.
Forskolin (0.1 μM), 8‐bromoguanosin 3′, 5′ cyclic monophosphate (8Br‐cGMP, 0.1 mM) and Y‐26763 (10 μM) suppressed contractions without reducing the amplitude of either action potentials or Ca transients.
This paper confirms that action potentials and associated calcium transients are fundamental mechanisms in generating spontaneous contractions in smooth muscles of the guinea‐pig bladder. However, in parallel with the excitation–contraction coupling, the sensitivity of the contractile proteins for Ca2+ may play an important role in regulating spontaneous excitation and can be modulated by cyclic nucleotides and Rho kinase.
British Journal of Pharmacology (2004) 141, 183–193. doi:10.1038/sj.bjp.0705602</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>14662721</pmid><doi>10.1038/sj.bjp.0705602</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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subjects | action potential Action Potentials - drug effects Action Potentials - physiology Amides - pharmacology Animals Apamin - pharmacology Biological and medical sciences Boron Compounds - pharmacology Ca sensitivity Calcium - chemistry Calcium - physiology Calcium Signaling - physiology Carbachol - pharmacology Charybdotoxin - pharmacology Colforsin - pharmacology Cromakalim - pharmacology Cyclic GMP - analogs & derivatives Cyclic GMP - pharmacology cyclic nucleotide Dose-Response Relationship, Drug Drug Synergism Female Fura-2 Guinea Pigs Isotonic Contraction - drug effects Isotonic Contraction - physiology Male Mechanotransduction, Cellular - physiology Medical sciences Microelectrodes Muscle Contraction - drug effects Muscle, Smooth - drug effects Muscle, Smooth - physiology Nifedipine - pharmacology Pharmacology. Drug treatments Pyridines - pharmacology Transducers Urinary bladder Urinary Bladder - cytology Urinary Bladder - drug effects Urinary Bladder - physiology |
title | Correlation between spontaneous electrical, calcium and mechanical activity in detrusor smooth muscle of the guinea‐pig bladder |
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