The properties of ryanodine‐sensitive Ca2+ release in mouse gastric smooth muscle cells
Under voltage‐clamped conditions, gastric smooth muscle cells of BALB/c mice developed spontaneous (STOCs) and caffeine‐ (ICAF) and carbachol‐induced (ICCh) transient outward currents. In fura‐2 microscopic measurements of intracellular Ca2+ concentration ([Ca2+]i), caffeine and carbachol (CCh) prov...
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| Veröffentlicht in: | British journal of pharmacology 2001-05, Vol.133 (1), p.125-137 |
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| description | Under voltage‐clamped conditions, gastric smooth muscle cells of BALB/c mice developed spontaneous (STOCs) and caffeine‐ (ICAF) and carbachol‐induced (ICCh) transient outward currents.
In fura‐2 microscopic measurements of intracellular Ca2+ concentration ([Ca2+]i), caffeine and carbachol (CCh) provoked similar transient [Ca2+]i elevations.
Both ICCh and CCh‐induced [Ca2+]i elevation of single smooth muscle cells occurred in an ‘all‐or‐nothing’ fashion in contrast to the reproducible caffeine responses.
On the basis of the suppression of STOCs and ICAF by nicardipine, tetraethylammonium and iberiotoxin, but not by charybdotoxin nor apamin, it was suggested that both currents were generated by large conductance type Ca2+‐activated K+ channels.
In measurements of isometric tension, caffeine produced relaxation of gastric smooth muscle strips in a concentration‐dependent manner (0.1 – 3 mM). The concentration‐dependent relaxation with caffeine was mimicked by dibutyryl cyclic AMP which produced potentiation of contraction triggered by 50 mM KCl.
At caffeine concentrations >3 mM, a transient contraction followed by relaxation was provoked as the quasi maximal response to caffeine. In the quasi maximal response, caffeine acted as a potent relaxant in smooth muscle strips precontracted with 50 mM KCl or 3 μM CCh.
The relaxation with caffeine was significantly accelerated in those strips precontracted with KCl or CCh. All these results suggest that ryanodine‐sensitive Ca2+ release, which is triggered by caffeine, is an important modifier of Ca2+ homeostasis in the cytoplasm and the contractility of gastric smooth muscle cells of mice.
British Journal of Pharmacology (2001) 133, 125–137; doi:10.1038/sj.bjp.0704048 |
| doi_str_mv | 10.1038/sj.bjp.0704048 |
| format | Article |
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In fura‐2 microscopic measurements of intracellular Ca2+ concentration ([Ca2+]i), caffeine and carbachol (CCh) provoked similar transient [Ca2+]i elevations.
Both ICCh and CCh‐induced [Ca2+]i elevation of single smooth muscle cells occurred in an ‘all‐or‐nothing’ fashion in contrast to the reproducible caffeine responses.
On the basis of the suppression of STOCs and ICAF by nicardipine, tetraethylammonium and iberiotoxin, but not by charybdotoxin nor apamin, it was suggested that both currents were generated by large conductance type Ca2+‐activated K+ channels.
In measurements of isometric tension, caffeine produced relaxation of gastric smooth muscle strips in a concentration‐dependent manner (0.1 – 3 mM). The concentration‐dependent relaxation with caffeine was mimicked by dibutyryl cyclic AMP which produced potentiation of contraction triggered by 50 mM KCl.
At caffeine concentrations >3 mM, a transient contraction followed by relaxation was provoked as the quasi maximal response to caffeine. In the quasi maximal response, caffeine acted as a potent relaxant in smooth muscle strips precontracted with 50 mM KCl or 3 μM CCh.
The relaxation with caffeine was significantly accelerated in those strips precontracted with KCl or CCh. All these results suggest that ryanodine‐sensitive Ca2+ release, which is triggered by caffeine, is an important modifier of Ca2+ homeostasis in the cytoplasm and the contractility of gastric smooth muscle cells of mice.
British Journal of Pharmacology (2001) 133, 125–137; doi:10.1038/sj.bjp.0704048</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1038/sj.bjp.0704048</identifier><identifier>PMID: 11325802</identifier><identifier>CODEN: BJPCBM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Biological and medical sciences ; Ca2+‐activated K+ current ; caffeine ; Cell membranes. Ionic channels. Membrane pores ; Cell structures and functions ; Fundamental and applied biological sciences. Psychology ; Gastric smooth muscle cell of mouse ; intracellular Ca2+ store ; isometric tension ; Molecular and cellular biology ; patch‐clamp technique ; ryanodine ; Stomach ; Vertebrates: digestive system</subject><ispartof>British journal of pharmacology, 2001-05, Vol.133 (1), p.125-137</ispartof><rights>2001 British Pharmacological Society</rights><rights>2001 INIST-CNRS</rights><rights>Copyright Nature Publishing Group May 2001</rights><rights>Copyright 2001, Nature Publishing Group 2001 Nature Publishing Group</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1572764/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1572764/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,1427,27901,27902,45550,45551,46384,46808,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1006263$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Tokutomi, Yoshiko</creatorcontrib><creatorcontrib>Tokutomi, Naofumi</creatorcontrib><creatorcontrib>Nishi, Katsuhide</creatorcontrib><title>The properties of ryanodine‐sensitive Ca2+ release in mouse gastric smooth muscle cells</title><title>British journal of pharmacology</title><description>Under voltage‐clamped conditions, gastric smooth muscle cells of BALB/c mice developed spontaneous (STOCs) and caffeine‐ (ICAF) and carbachol‐induced (ICCh) transient outward currents.
In fura‐2 microscopic measurements of intracellular Ca2+ concentration ([Ca2+]i), caffeine and carbachol (CCh) provoked similar transient [Ca2+]i elevations.
Both ICCh and CCh‐induced [Ca2+]i elevation of single smooth muscle cells occurred in an ‘all‐or‐nothing’ fashion in contrast to the reproducible caffeine responses.
On the basis of the suppression of STOCs and ICAF by nicardipine, tetraethylammonium and iberiotoxin, but not by charybdotoxin nor apamin, it was suggested that both currents were generated by large conductance type Ca2+‐activated K+ channels.
In measurements of isometric tension, caffeine produced relaxation of gastric smooth muscle strips in a concentration‐dependent manner (0.1 – 3 mM). The concentration‐dependent relaxation with caffeine was mimicked by dibutyryl cyclic AMP which produced potentiation of contraction triggered by 50 mM KCl.
At caffeine concentrations >3 mM, a transient contraction followed by relaxation was provoked as the quasi maximal response to caffeine. In the quasi maximal response, caffeine acted as a potent relaxant in smooth muscle strips precontracted with 50 mM KCl or 3 μM CCh.
The relaxation with caffeine was significantly accelerated in those strips precontracted with KCl or CCh. All these results suggest that ryanodine‐sensitive Ca2+ release, which is triggered by caffeine, is an important modifier of Ca2+ homeostasis in the cytoplasm and the contractility of gastric smooth muscle cells of mice.
British Journal of Pharmacology (2001) 133, 125–137; doi:10.1038/sj.bjp.0704048</description><subject>Biological and medical sciences</subject><subject>Ca2+‐activated K+ current</subject><subject>caffeine</subject><subject>Cell membranes. Ionic channels. Membrane pores</subject><subject>Cell structures and functions</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastric smooth muscle cell of mouse</subject><subject>intracellular Ca2+ store</subject><subject>isometric tension</subject><subject>Molecular and cellular biology</subject><subject>patch‐clamp technique</subject><subject>ryanodine</subject><subject>Stomach</subject><subject>Vertebrates: digestive system</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpVkU1r3DAQhkVJaTZprzmLkFvwViNZH3spNEvTFALtIT30JGR5nJWxLUeyE_bWn9Df2F9SlyyhPc3A-_DOwEPIGbA1MGHe53ZdteOaaVay0rwiKyi1KqQwcERWjDFdABhzTE5ybhlbQi3fkGMAwaVhfEV-3O2QjimOmKaAmcaGpr0bYh0G_P3zV8Yhhyk8It06fkkTdugy0jDQPs7Lcu_ylIKnuY9x2tF-zr5D6rHr8lvyunFdxneHeUq-X3-6294Ut18_f9l-vC1GbpgpqroRIESpQXKovN74BmS1aWRjSlRVLSrRCGWk9qi1E054s1EMONM1ViikOCUfnnvHueqx9jhMyXV2TKF3aW-jC_b_ZAg7ex8fLUjNtSqXgvNDQYoPM-bJtnFOw_Kz5aDBQKnUAl0cIJe965rkBh_yyxVgTHElFkw8Y0-hw_0_sf1ry-bWLrbswZa9-nbD2caIPy13iyo</recordid><startdate>200105</startdate><enddate>200105</enddate><creator>Tokutomi, Yoshiko</creator><creator>Tokutomi, Naofumi</creator><creator>Nishi, Katsuhide</creator><general>Blackwell Publishing Ltd</general><general>Nature Publishing</general><scope>IQODW</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>5PM</scope></search><sort><creationdate>200105</creationdate><title>The properties of ryanodine‐sensitive Ca2+ release in mouse gastric smooth muscle cells</title><author>Tokutomi, Yoshiko ; Tokutomi, Naofumi ; Nishi, Katsuhide</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2808-bdf3133471521bc79cf15b9f5f84e6bd3b3f36857ce77a3a3c89601207debe353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Biological and medical sciences</topic><topic>Ca2+‐activated K+ current</topic><topic>caffeine</topic><topic>Cell membranes. Ionic channels. Membrane pores</topic><topic>Cell structures and functions</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastric smooth muscle cell of mouse</topic><topic>intracellular Ca2+ store</topic><topic>isometric tension</topic><topic>Molecular and cellular biology</topic><topic>patch‐clamp technique</topic><topic>ryanodine</topic><topic>Stomach</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tokutomi, Yoshiko</creatorcontrib><creatorcontrib>Tokutomi, Naofumi</creatorcontrib><creatorcontrib>Nishi, Katsuhide</creatorcontrib><collection>Pascal-Francis</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tokutomi, Yoshiko</au><au>Tokutomi, Naofumi</au><au>Nishi, Katsuhide</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The properties of ryanodine‐sensitive Ca2+ release in mouse gastric smooth muscle cells</atitle><jtitle>British journal of pharmacology</jtitle><date>2001-05</date><risdate>2001</risdate><volume>133</volume><issue>1</issue><spage>125</spage><epage>137</epage><pages>125-137</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><coden>BJPCBM</coden><abstract>Under voltage‐clamped conditions, gastric smooth muscle cells of BALB/c mice developed spontaneous (STOCs) and caffeine‐ (ICAF) and carbachol‐induced (ICCh) transient outward currents.
In fura‐2 microscopic measurements of intracellular Ca2+ concentration ([Ca2+]i), caffeine and carbachol (CCh) provoked similar transient [Ca2+]i elevations.
Both ICCh and CCh‐induced [Ca2+]i elevation of single smooth muscle cells occurred in an ‘all‐or‐nothing’ fashion in contrast to the reproducible caffeine responses.
On the basis of the suppression of STOCs and ICAF by nicardipine, tetraethylammonium and iberiotoxin, but not by charybdotoxin nor apamin, it was suggested that both currents were generated by large conductance type Ca2+‐activated K+ channels.
In measurements of isometric tension, caffeine produced relaxation of gastric smooth muscle strips in a concentration‐dependent manner (0.1 – 3 mM). The concentration‐dependent relaxation with caffeine was mimicked by dibutyryl cyclic AMP which produced potentiation of contraction triggered by 50 mM KCl.
At caffeine concentrations >3 mM, a transient contraction followed by relaxation was provoked as the quasi maximal response to caffeine. In the quasi maximal response, caffeine acted as a potent relaxant in smooth muscle strips precontracted with 50 mM KCl or 3 μM CCh.
The relaxation with caffeine was significantly accelerated in those strips precontracted with KCl or CCh. All these results suggest that ryanodine‐sensitive Ca2+ release, which is triggered by caffeine, is an important modifier of Ca2+ homeostasis in the cytoplasm and the contractility of gastric smooth muscle cells of mice.
British Journal of Pharmacology (2001) 133, 125–137; doi:10.1038/sj.bjp.0704048</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>11325802</pmid><doi>10.1038/sj.bjp.0704048</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Ca2+‐activated K+ current caffeine Cell membranes. Ionic channels. Membrane pores Cell structures and functions Fundamental and applied biological sciences. Psychology Gastric smooth muscle cell of mouse intracellular Ca2+ store isometric tension Molecular and cellular biology patch‐clamp technique ryanodine Stomach Vertebrates: digestive system |
| title | The properties of ryanodine‐sensitive Ca2+ release in mouse gastric smooth muscle cells |
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