Influence of gender and the oestrous cycle on in vitro contractile responses of the rat urinary bladder to cholinergic stimulation
Experiments were done to determine the influence of gender and the oestrous cycle on rat urinary bladder contractility in response to cholinergic stimulation. Bladder strips from female rats responded to high frequency stimulation with smaller contractile responses than did strips from males, and to...
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| description | Experiments were done to determine the influence of gender and the oestrous cycle on rat urinary bladder contractility in response to cholinergic stimulation.
Bladder strips from female rats responded to high frequency stimulation with smaller contractile responses than did strips from males, and to low concentrations of carbachol with greater responses. The decreased responsiveness of bladder strips from female rats to electrical field stimulation can be primarily attributed to the rats in the oestrous stage of the oestrous cycle.
Bladder strips from female rats in all stages of the oestrous cycle were more sensitive to carbachol than those from males, but there were no differences in sensitivity to electrical field stimulation.
The contractile responses of strips from both male and female rats to carbachol were antagonized by muscarinic antagonists with the following rank order of affinity (pA2) estimates: 4‐DAMP>>pirenzepine>methoctramine, suggesting that the receptor mediating contraction was the M3 subtype. There were no differences in pA2 values between bladder strips from male and female rats.
The data indicate that responsiveness of bladder strips to electrical field stimulation and carbachol is altered in female rats in the oestrous stage of the oestrous cycle. Furthermore, gender influences the sensitivity of rat bladder to muscarinic stimulation.
British Journal of Pharmacology (2000) 131, 177–184; doi:10.1038/sj.bjp.0703551 |
| doi_str_mv | 10.1038/sj.bjp.0703551 |
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Bladder strips from female rats responded to high frequency stimulation with smaller contractile responses than did strips from males, and to low concentrations of carbachol with greater responses. The decreased responsiveness of bladder strips from female rats to electrical field stimulation can be primarily attributed to the rats in the oestrous stage of the oestrous cycle.
Bladder strips from female rats in all stages of the oestrous cycle were more sensitive to carbachol than those from males, but there were no differences in sensitivity to electrical field stimulation.
The contractile responses of strips from both male and female rats to carbachol were antagonized by muscarinic antagonists with the following rank order of affinity (pA2) estimates: 4‐DAMP>>pirenzepine>methoctramine, suggesting that the receptor mediating contraction was the M3 subtype. There were no differences in pA2 values between bladder strips from male and female rats.
The data indicate that responsiveness of bladder strips to electrical field stimulation and carbachol is altered in female rats in the oestrous stage of the oestrous cycle. Furthermore, gender influences the sensitivity of rat bladder to muscarinic stimulation.
British Journal of Pharmacology (2000) 131, 177–184; doi:10.1038/sj.bjp.0703551</description><identifier>ISSN: 0007-1188</identifier><identifier>EISSN: 1476-5381</identifier><identifier>DOI: 10.1038/sj.bjp.0703551</identifier><identifier>PMID: 10991909</identifier><identifier>CODEN: BJPCBM</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Biological and medical sciences ; Carbachol - pharmacology ; Cholinergic Agonists - pharmacology ; Cholinergic system ; Diamines - pharmacology ; Electric Stimulation ; electrical field stimulation ; Estrus - drug effects ; Estrus - physiology ; Female ; Gender ; Male ; Medical sciences ; Muscarinic Antagonists - pharmacology ; muscarinic receptors ; Nephrology. Urinary tract diseases ; Neuropharmacology ; Neurotransmitters. Neurotransmission. Receptors ; oestrous cycle ; Parasympatholytics - pharmacology ; Pharmacology. Drug treatments ; Piperidines - pharmacology ; Pirenzepine - pharmacology ; Rats ; Rats, Sprague-Dawley ; Receptors, Cholinergic - metabolism ; Sex Characteristics ; smooth muscle contraction ; Time Factors ; urinary bladder ; Urinary Bladder - drug effects ; Urinary Bladder - physiology ; Urinary system involvement in other diseases. Miscellaneous ; Urinary tract. Prostate gland ; Uterine Contraction - drug effects ; Uterine Contraction - physiology</subject><ispartof>British journal of pharmacology, 2000-09, Vol.131 (2), p.177-184</ispartof><rights>2000 British Pharmacological Society</rights><rights>2000 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Sep 2000</rights><rights>Copyright 2000, Nature Publishing Group 2000 Nature Publishing Group</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5523-2e92622acf2b972e84453e54219044adf1e17935e957433cd2bf19ff82d8dd313</citedby><cites>FETCH-LOGICAL-c5523-2e92622acf2b972e84453e54219044adf1e17935e957433cd2bf19ff82d8dd313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1572312/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1572312/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,1417,1433,27924,27925,45574,45575,46409,46833,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1503561$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10991909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Longhurst, Penelope A</creatorcontrib><creatorcontrib>Levendusky, Mark</creatorcontrib><title>Influence of gender and the oestrous cycle on in vitro contractile responses of the rat urinary bladder to cholinergic stimulation</title><title>British journal of pharmacology</title><addtitle>Br J Pharmacol</addtitle><description>Experiments were done to determine the influence of gender and the oestrous cycle on rat urinary bladder contractility in response to cholinergic stimulation.
Bladder strips from female rats responded to high frequency stimulation with smaller contractile responses than did strips from males, and to low concentrations of carbachol with greater responses. The decreased responsiveness of bladder strips from female rats to electrical field stimulation can be primarily attributed to the rats in the oestrous stage of the oestrous cycle.
Bladder strips from female rats in all stages of the oestrous cycle were more sensitive to carbachol than those from males, but there were no differences in sensitivity to electrical field stimulation.
The contractile responses of strips from both male and female rats to carbachol were antagonized by muscarinic antagonists with the following rank order of affinity (pA2) estimates: 4‐DAMP>>pirenzepine>methoctramine, suggesting that the receptor mediating contraction was the M3 subtype. There were no differences in pA2 values between bladder strips from male and female rats.
The data indicate that responsiveness of bladder strips to electrical field stimulation and carbachol is altered in female rats in the oestrous stage of the oestrous cycle. Furthermore, gender influences the sensitivity of rat bladder to muscarinic stimulation.
British Journal of Pharmacology (2000) 131, 177–184; doi:10.1038/sj.bjp.0703551</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carbachol - pharmacology</subject><subject>Cholinergic Agonists - pharmacology</subject><subject>Cholinergic system</subject><subject>Diamines - pharmacology</subject><subject>Electric Stimulation</subject><subject>electrical field stimulation</subject><subject>Estrus - drug effects</subject><subject>Estrus - physiology</subject><subject>Female</subject><subject>Gender</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Muscarinic Antagonists - pharmacology</subject><subject>muscarinic receptors</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Neuropharmacology</subject><subject>Neurotransmitters. Neurotransmission. Receptors</subject><subject>oestrous cycle</subject><subject>Parasympatholytics - pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Piperidines - pharmacology</subject><subject>Pirenzepine - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Cholinergic - metabolism</subject><subject>Sex Characteristics</subject><subject>smooth muscle contraction</subject><subject>Time Factors</subject><subject>urinary bladder</subject><subject>Urinary Bladder - drug effects</subject><subject>Urinary Bladder - physiology</subject><subject>Urinary system involvement in other diseases. Miscellaneous</subject><subject>Urinary tract. Prostate gland</subject><subject>Uterine Contraction - drug effects</subject><subject>Uterine Contraction - physiology</subject><issn>0007-1188</issn><issn>1476-5381</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUGP0zAQhS0EYsvClSOyEOKW4rHjOrkgwQrYlVaCA5wtx7FbR6ld7GRRr_xyJmoFCxdOlme-eX7jR8hzYGtgonlThnU3HNZMMSElPCArqNWmkqKBh2TFGFMVQNNckCelDIxhU8nH5AJY20LL2hX5eRP9OLtoHU2ebl3sXaYm9nTaYcWVKae5UHu0I14jDZHeBaxRm-KUjZ0C1rMrhxSLK4vEMpfNROccoslH2o2mXzQnnNmlMUSXt8HSMoX9PJoppPiUPPJmLO7Z-bwk3z5--Hp1Xd1-_nRz9e62slJyUXHX8g3nxnretYq7pq6lcLLmuEhdm96DA9UK6VqpaiFszzsPrfcN75u-FyAuyduT7mHu9q63btlg1Icc9mhUJxP0350Ydnqb7jRIxQVwFHh9Fsjp-4x_o_ehWDeOJjr8Ja04VyClQPDlP-CQ5hxxOc1BgZJqUyO0PkE2p1Ky87-dANNLtroMGrPV52xx4MV9__fwU5gIvDoDplgz-myiDeUPJ1Fns-iIE_YDwzv-51X9_ss1oFvxC1Cxv_8</recordid><startdate>200009</startdate><enddate>200009</enddate><creator>Longhurst, Penelope A</creator><creator>Levendusky, Mark</creator><general>Blackwell Publishing Ltd</general><general>Nature Publishing</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>200009</creationdate><title>Influence of gender and the oestrous cycle on in vitro contractile responses of the rat urinary bladder to cholinergic stimulation</title><author>Longhurst, Penelope A ; Levendusky, Mark</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5523-2e92622acf2b972e84453e54219044adf1e17935e957433cd2bf19ff82d8dd313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbachol - pharmacology</topic><topic>Cholinergic Agonists - pharmacology</topic><topic>Cholinergic system</topic><topic>Diamines - pharmacology</topic><topic>Electric Stimulation</topic><topic>electrical field stimulation</topic><topic>Estrus - drug effects</topic><topic>Estrus - physiology</topic><topic>Female</topic><topic>Gender</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Muscarinic Antagonists - pharmacology</topic><topic>muscarinic receptors</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Neuropharmacology</topic><topic>Neurotransmitters. Neurotransmission. Receptors</topic><topic>oestrous cycle</topic><topic>Parasympatholytics - pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Piperidines - pharmacology</topic><topic>Pirenzepine - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Cholinergic - metabolism</topic><topic>Sex Characteristics</topic><topic>smooth muscle contraction</topic><topic>Time Factors</topic><topic>urinary bladder</topic><topic>Urinary Bladder - drug effects</topic><topic>Urinary Bladder - physiology</topic><topic>Urinary system involvement in other diseases. Miscellaneous</topic><topic>Urinary tract. Prostate gland</topic><topic>Uterine Contraction - drug effects</topic><topic>Uterine Contraction - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Longhurst, Penelope A</creatorcontrib><creatorcontrib>Levendusky, Mark</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>British journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Longhurst, Penelope A</au><au>Levendusky, Mark</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of gender and the oestrous cycle on in vitro contractile responses of the rat urinary bladder to cholinergic stimulation</atitle><jtitle>British journal of pharmacology</jtitle><addtitle>Br J Pharmacol</addtitle><date>2000-09</date><risdate>2000</risdate><volume>131</volume><issue>2</issue><spage>177</spage><epage>184</epage><pages>177-184</pages><issn>0007-1188</issn><eissn>1476-5381</eissn><coden>BJPCBM</coden><abstract>Experiments were done to determine the influence of gender and the oestrous cycle on rat urinary bladder contractility in response to cholinergic stimulation.
Bladder strips from female rats responded to high frequency stimulation with smaller contractile responses than did strips from males, and to low concentrations of carbachol with greater responses. The decreased responsiveness of bladder strips from female rats to electrical field stimulation can be primarily attributed to the rats in the oestrous stage of the oestrous cycle.
Bladder strips from female rats in all stages of the oestrous cycle were more sensitive to carbachol than those from males, but there were no differences in sensitivity to electrical field stimulation.
The contractile responses of strips from both male and female rats to carbachol were antagonized by muscarinic antagonists with the following rank order of affinity (pA2) estimates: 4‐DAMP>>pirenzepine>methoctramine, suggesting that the receptor mediating contraction was the M3 subtype. There were no differences in pA2 values between bladder strips from male and female rats.
The data indicate that responsiveness of bladder strips to electrical field stimulation and carbachol is altered in female rats in the oestrous stage of the oestrous cycle. Furthermore, gender influences the sensitivity of rat bladder to muscarinic stimulation.
British Journal of Pharmacology (2000) 131, 177–184; doi:10.1038/sj.bjp.0703551</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10991909</pmid><doi>10.1038/sj.bjp.0703551</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Free Content; Access via Wiley Online Library; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Carbachol - pharmacology Cholinergic Agonists - pharmacology Cholinergic system Diamines - pharmacology Electric Stimulation electrical field stimulation Estrus - drug effects Estrus - physiology Female Gender Male Medical sciences Muscarinic Antagonists - pharmacology muscarinic receptors Nephrology. Urinary tract diseases Neuropharmacology Neurotransmitters. Neurotransmission. Receptors oestrous cycle Parasympatholytics - pharmacology Pharmacology. Drug treatments Piperidines - pharmacology Pirenzepine - pharmacology Rats Rats, Sprague-Dawley Receptors, Cholinergic - metabolism Sex Characteristics smooth muscle contraction Time Factors urinary bladder Urinary Bladder - drug effects Urinary Bladder - physiology Urinary system involvement in other diseases. Miscellaneous Urinary tract. Prostate gland Uterine Contraction - drug effects Uterine Contraction - physiology |
| title | Influence of gender and the oestrous cycle on in vitro contractile responses of the rat urinary bladder to cholinergic stimulation |
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